Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,894,854 G→C 8.7% intergenic (+83/+65) ygcP → / ← ygcQ putative antiterminator regulatory protein/putative flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,894,8540GC 8.7% 261.9 / 14.3 80intergenic (+83/+65)ygcP/ygcQputative antiterminator regulatory protein/putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (29/44);  new base C (3/4);  total (32/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-01

GTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGC  >  NC_000913/2894710‑2894996
                                                                                                                                                |                                                                                                                                              
gtgtCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCCGCGCCTGATGCGACGCTTGAcgc                                                                                                                                            >  2:1209032/1‑149 (MQ=255)
   tCGTGTCGCTTTCCACCAAGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc                                                                                                                                         <  2:1168509/149‑1 (MQ=255)
   tCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc                                                                                                                                         <  2:655174/149‑1 (MQ=255)
    cGTGGCGCTTTCCACCACGTATTCCGGGGTCTGGACGTTAGAGATAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCt                                                                                                                                        <  1:54676/149‑1 (MQ=255)
       ggCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                                                                     <  1:705537/149‑1 (MQ=255)
       ggCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                                                                     <  2:488633/149‑1 (MQ=255)
        gcgcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATCGAATTTGGTTTGATTTGTAGGCAGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                                                                    >  2:605893/1‑149 (MQ=255)
          gcTTTCCACCACGAATACCGGGGTCTGGACGTTATCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                  <  2:1324923/149‑1 (MQ=255)
          gcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                  <  1:1263707/149‑1 (MQ=255)
           cTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATACTCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAgg                                                                                                                                 <  1:822586/149‑1 (MQ=255)
            tttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATACGAAATTCAGCGCCTGATGCGACCATTGCCGCGTCTTATCCGGc                                                                                                                                >  2:258277/1‑149 (MQ=255)
            tttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACGTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCAACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGc                                                                                                                                >  1:247068/1‑149 (MQ=255)
             ttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcg                                                                                                                               <  1:83477/149‑2 (MQ=255)
             ttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcg                                                                                                                               <  2:1341837/149‑2 (MQ=255)
                 accacGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACa                                                                                                                           <  2:397705/149‑1 (MQ=255)
                           ccGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg                                                                                                                 >  1:1389057/1‑149 (MQ=255)
                           ccGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg                                                                                                                 <  2:942678/149‑1 (MQ=255)
                            gggggTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAAGCACTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCTGGCCTACAAGTCCCCAGGc                                                                                                                <  2:1576593/148‑1 (MQ=255)
                            cGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc                                                                                                                <  2:47722/149‑1 (MQ=255)
                            cGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc                                                                                                                <  2:1499682/149‑1 (MQ=255)
                               ggTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAg                                                                                                             <  2:755425/149‑1 (MQ=255)
                                 tCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGaa                                                                                                           <  2:729478/149‑1 (MQ=255)
                                  cTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAc                                                                                                          <  1:457677/149‑1 (MQ=255)
                                     gACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGACTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGt                                                                                                       >  2:1170242/1‑149 (MQ=255)
                                      aCGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTa                                                                                                      <  1:327456/149‑1 (MQ=255)
                                       cGTTCGCGAAAAAATTACTTTGACGGGATAATCGTATCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAg                                                                                                     <  1:974041/149‑1 (MQ=255)
                                          tAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa                                                                                                  >  2:1540732/1‑149 (MQ=255)
                                          tAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa                                                                                                  >  1:1076307/1‑149 (MQ=255)
                                          tAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGACGCACGACAAATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTATTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa                                                                                                  >  1:287346/1‑149 (MQ=255)
                                           aGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAg                                                                                                 <  2:1349389/149‑1 (MQ=255)
                                                  aaaTTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcatc                                                                                          <  1:982560/149‑1 (MQ=255)
                                                  aaaTTACTTTGACGGGATAATCGTAACCAATTGAATTTGGATTGATTTGTAGGCCGCACGCCACATACGACATACAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcatc                                                                                          >  2:640816/1‑149 (MQ=255)
                                                      tACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGc                                                                                      <  1:230089/149‑1 (MQ=255)
                                                         tttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                   >  2:52047/1‑149 (MQ=255)
                                                         tttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                   >  2:1427781/1‑149 (MQ=255)
                                                         tttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGAAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                   >  2:6847/1‑149 (MQ=255)
                                                                 gATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTAtt                                                                           <  2:212204/149‑1 (MQ=255)
                                                                  aTAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTg                                                                          <  2:678428/149‑1 (MQ=255)
                                                                  aTAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACATTATTg                                                                          <  1:553564/149‑1 (MQ=255)
                                                                   tAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACAGTAGGCAGGATCATCCGGCAACACGTTATTgg                                                                         >  1:1098419/1‑149 (MQ=255)
                                                                    aaTCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGc                                                                        >  2:952641/1‑149 (MQ=255)
                                                                      tCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGAGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACAAGTTATTGGCaa                                                                      >  1:1138092/1‑149 (MQ=255)
                                                                       cGTAACCAATTGAATTTGGTTTGAGTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAAt                                                                     <  2:267320/149‑1 (MQ=255)
                                                                         tAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCATAGGCAGGATCATCCGGCAACACGTTATTGGCAATCt                                                                   >  1:739273/1‑149 (MQ=255)
                                                                            ccAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCg                                                                <  2:247068/149‑1 (MQ=255)
                                                                            ccAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCg                                                                >  1:1315451/1‑149 (MQ=255)
                                                                             cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGt                                                               <  1:1335449/149‑1 (MQ=255)
                                                                              aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACTCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                              >  2:1416982/1‑149 (MQ=255)
                                                                              aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                              >  1:632496/1‑149 (MQ=255)
                                                                              aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                              >  2:561762/1‑149 (MQ=255)
                                                                                 tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGAGATTAAGCGCCTGATGCGACGCTTGAAGCGTCTTATCAGGCCTACAAGTCACCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                           <  1:258277/149‑1 (MQ=255)
                                                                                 tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                           <  2:1117652/149‑1 (MQ=255)
                                                                                 tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                           <  2:1111966/149‑1 (MQ=255)
                                                                                 tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCATAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                           <  2:739273/149‑1 (MQ=255)
                                                                                  gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATg                                                          >  1:959179/1‑149 (MQ=255)
                                                                                     tttGGTTTGATTTGTAGGCCGCCCGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                       <  1:1224858/149‑1 (MQ=255)
                                                                                     tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                       <  1:429327/149‑1 (MQ=255)
                                                                                     tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                       <  2:737363/149‑1 (MQ=255)
                                                                                     tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                       <  1:561762/149‑1 (MQ=255)
                                                                                        ggTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc                                                    <  1:1209032/149‑1 (MQ=255)
                                                                                            tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCa                                                <  1:1393105/149‑1 (MQ=255)
                                                                                              aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg                                                                                                    >  1:1543062/1‑95 (MQ=255)
                                                                                              aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg                                                                                                    <  2:1543062/95‑1 (MQ=255)
                                                                                              aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCTATCTGCGTGAATGTTTGTCACCAGt                                              >  1:795494/1‑149 (MQ=255)
                                                                                                      gCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAg                                      <  1:493489/149‑1 (MQ=255)
                                                                                                        cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGca                                    >  1:921490/1‑149 (MQ=255)
                                                                                                        cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGca                                    >  1:159232/1‑149 (MQ=255)
                                                                                                         gCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGcac                                   <  1:48226/149‑1 (MQ=255)
                                                                                                                   tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAg                         >  1:1079845/1‑149 (MQ=255)
                                                                                                                     cGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtc                       <  1:952641/149‑1 (MQ=255)
                                                                                                                      gACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtca                      <  1:868495/149‑1 (MQ=255)
                                                                                                                        cATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtcatc                    <  1:52047/149‑1 (MQ=255)
                                                                                                                            cAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAAcc                <  2:1287148/149‑1 (MQ=255)
                                                                                                                                gcCTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGc            >  1:48091/1‑149 (MQ=255)
                                                                                                                                  cTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGACCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCa          >  2:1424110/1‑149 (MQ=255)
                                                                                                                                  cTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCaa                  >  2:1415992/1‑141 (MQ=255)
                                                                                                                                  cTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCaa                  <  1:1415992/141‑1 (MQ=255)
                                                                                                                                    gataagACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAAc        >  1:920832/6‑149 (MQ=255)
                                                                                                                                          ccGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCTATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGc  <  2:258113/148‑1 (MQ=255)
                                                                                                                                          aCGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGc  >  2:959185/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGC  >  NC_000913/2894710‑2894996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: