Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,894,863 T→C 8.8% intergenic (+92/+56) ygcP → / ← ygcQ putative antiterminator regulatory protein/putative flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,894,8630TC 8.8% 228.9 / 10.2 79intergenic (+92/+56)ygcP/ygcQputative antiterminator regulatory protein/putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (28/44);  new base C (3/4);  total (31/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGCCTGTGAAAATACC  >  NC_000913/2894717‑2895009
                                                                                                                                                  |                                                                                                                                                  
ggCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                                                                                  <  2:488633/149‑1 (MQ=255)
ggCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                                                                                  <  1:705537/149‑1 (MQ=255)
 gcgcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATCGAATTTGGTTTGATTTGTAGGCAGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                                                                                 >  2:605893/1‑149 (MQ=255)
   gcTTTCCACCACGAATACCGGGGTCTGGACGTTATCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                               <  2:1324923/149‑1 (MQ=255)
   gcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                               <  1:1263707/149‑1 (MQ=255)
    cTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATACTCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAgg                                                                                                                                              <  1:822586/149‑1 (MQ=255)
     tttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATACGAAATTCAGCGCCTGATGCGACCATTGCCGCGTCTTATCCGGc                                                                                                                                             >  2:258277/1‑149 (MQ=255)
     tttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACGTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCAACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGc                                                                                                                                             >  1:247068/1‑149 (MQ=255)
      ttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcg                                                                                                                                            <  1:83477/149‑2 (MQ=255)
      ttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcg                                                                                                                                            <  2:1341837/149‑2 (MQ=255)
          accacGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACa                                                                                                                                        <  2:397705/149‑1 (MQ=255)
                    ccGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg                                                                                                                              >  1:1389057/1‑149 (MQ=255)
                    ccGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg                                                                                                                              <  2:942678/149‑1 (MQ=255)
                     gggggTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAAGCACTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCTGGCCTACAAGTCCCCAGGc                                                                                                                             <  2:1576593/148‑1 (MQ=255)
                     cGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc                                                                                                                             <  2:1499682/149‑1 (MQ=255)
                     cGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc                                                                                                                             <  2:47722/149‑1 (MQ=255)
                        ggTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAg                                                                                                                          <  2:755425/149‑1 (MQ=255)
                          tCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGaa                                                                                                                        <  2:729478/149‑1 (MQ=255)
                           cTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAc                                                                                                                       <  1:457677/149‑1 (MQ=255)
                              gACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGACTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGt                                                                                                                    >  2:1170242/1‑149 (MQ=255)
                               aCGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTa                                                                                                                   <  1:327456/149‑1 (MQ=255)
                                cGTTCGCGAAAAAATTACTTTGACGGGATAATCGTATCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAg                                                                                                                  <  1:974041/149‑1 (MQ=255)
                                   tAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa                                                                                                               >  2:1540732/1‑149 (MQ=255)
                                   tAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa                                                                                                               >  1:1076307/1‑149 (MQ=255)
                                   tAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGACGCACGACAAATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTATTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa                                                                                                               >  1:287346/1‑149 (MQ=255)
                                    aGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAg                                                                                                              <  2:1349389/149‑1 (MQ=255)
                                           aaaTTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcatc                                                                                                       <  1:982560/149‑1 (MQ=255)
                                           aaaTTACTTTGACGGGATAATCGTAACCAATTGAATTTGGATTGATTTGTAGGCCGCACGCCACATACGACATACAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcatc                                                                                                       >  2:640816/1‑149 (MQ=255)
                                               tACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGc                                                                                                   <  1:230089/149‑1 (MQ=255)
                                                  tttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                                >  2:52047/1‑149 (MQ=255)
                                                  tttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                                >  2:1427781/1‑149 (MQ=255)
                                                  tttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGAAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                                >  2:6847/1‑149 (MQ=255)
                                                          gATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTAtt                                                                                        <  2:212204/149‑1 (MQ=255)
                                                           aTAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTg                                                                                       <  2:678428/149‑1 (MQ=255)
                                                           aTAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACATTATTg                                                                                       <  1:553564/149‑1 (MQ=255)
                                                            tAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACAGTAGGCAGGATCATCCGGCAACACGTTATTgg                                                                                      >  1:1098419/1‑149 (MQ=255)
                                                             aaTCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGc                                                                                     >  2:952641/1‑149 (MQ=255)
                                                               tCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGAGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACAAGTTATTGGCaa                                                                                   >  1:1138092/1‑149 (MQ=255)
                                                                cGTAACCAATTGAATTTGGTTTGAGTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAAt                                                                                  <  2:267320/149‑1 (MQ=255)
                                                                  tAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCATAGGCAGGATCATCCGGCAACACGTTATTGGCAATCt                                                                                >  1:739273/1‑149 (MQ=255)
                                                                     ccAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCg                                                                             <  2:247068/149‑1 (MQ=255)
                                                                     ccAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCg                                                                             >  1:1315451/1‑149 (MQ=255)
                                                                      cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGt                                                                            <  1:1335449/149‑1 (MQ=255)
                                                                       aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACTCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                                           >  2:1416982/1‑149 (MQ=255)
                                                                       aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                                           >  1:632496/1‑149 (MQ=255)
                                                                       aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                                           >  2:561762/1‑149 (MQ=255)
                                                                          tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGAGATTAAGCGCCTGATGCGACGCTTGAAGCGTCTTATCAGGCCTACAAGTCACCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                                        <  1:258277/149‑1 (MQ=255)
                                                                          tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                                        <  2:1117652/149‑1 (MQ=255)
                                                                          tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                                        <  2:1111966/149‑1 (MQ=255)
                                                                          tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCATAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                                        <  2:739273/149‑1 (MQ=255)
                                                                           gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATg                                                                       >  1:959179/1‑149 (MQ=255)
                                                                              tttGGTTTGATTTGTAGGCCGCCCGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                                    <  1:1224858/149‑1 (MQ=255)
                                                                              tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                                    <  2:737363/149‑1 (MQ=255)
                                                                              tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                                    <  1:561762/149‑1 (MQ=255)
                                                                              tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                                    <  1:429327/149‑1 (MQ=255)
                                                                                 ggTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc                                                                 <  1:1209032/149‑1 (MQ=255)
                                                                                     tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCa                                                             <  1:1393105/149‑1 (MQ=255)
                                                                                       aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg                                                                                                                 <  2:1543062/95‑1 (MQ=255)
                                                                                       aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg                                                                                                                 >  1:1543062/1‑95 (MQ=255)
                                                                                       aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCTATCTGCGTGAATGTTTGTCACCAGt                                                           >  1:795494/1‑149 (MQ=255)
                                                                                               gCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAg                                                   <  1:493489/149‑1 (MQ=255)
                                                                                                 cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGca                                                 >  1:921490/1‑149 (MQ=255)
                                                                                                 cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGca                                                 >  1:159232/1‑149 (MQ=255)
                                                                                                  gCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGcac                                                <  1:48226/149‑1 (MQ=255)
                                                                                                            tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAg                                      >  1:1079845/1‑149 (MQ=255)
                                                                                                              cGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtc                                    <  1:952641/149‑1 (MQ=255)
                                                                                                               gACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtca                                   <  1:868495/149‑1 (MQ=255)
                                                                                                                 cATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtcatc                                 <  1:52047/149‑1 (MQ=255)
                                                                                                                     cAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAAcc                             <  2:1287148/149‑1 (MQ=255)
                                                                                                                         gcCTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGc                         >  1:48091/1‑149 (MQ=255)
                                                                                                                           cTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGACCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCa                       >  2:1424110/1‑149 (MQ=255)
                                                                                                                           cTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCaa                               >  2:1415992/1‑141 (MQ=255)
                                                                                                                           cTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCaa                               <  1:1415992/141‑1 (MQ=255)
                                                                                                                             gataagACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAAc                     >  1:920832/6‑149 (MQ=255)
                                                                                                                                   ccGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCTATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGc               <  2:258113/148‑1 (MQ=255)
                                                                                                                                   aCGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGc               >  2:959185/1‑149 (MQ=255)
                                                                                                                                                cTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACTTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGCCTGTGAAAATAcc  <  2:287346/149‑1 (MQ=255)
                                                                                                                                                cTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGCCTGTGAAAATAcc  <  2:1076307/149‑1 (MQ=255)
                                                                                                                                                cTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGGTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGCCTGTGAAAATAcc  <  2:72983/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGCCTGTGAAAATACC  >  NC_000913/2894717‑2895009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: