Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,995,558 T→G 6.2% pseudogene (203/447 nt) ygeN ← pseudogene, orgB family, part of T3SS PAI ETT2 remnant

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,995,5580TG 6.2% 571.5 / 22.6 189pseudogene (203/447 nt)ygeNpseudogene, orgB family, part of T3SS PAI ETT2 remnant
Reads supporting (aligned to +/- strand):  ref base T (84/93);  new base G (6/6);  total (90/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

Only 100 of 195 total aligned reads displayed.

GATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCT  >  NC_000913/2995410‑2995704
                                                                                                                                                    |                                                                                                                                                  
 aTTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACCAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACAtt                                                                                                                                                   <  1:161538/149‑1 (MQ=255)
 aTTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAACATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACAtt                                                                                                                                                   >  2:1160711/1‑149 (MQ=255)
       ttAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCatat                                                                                                                                             >  2:261837/1‑149 (MQ=255)
        tAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATAtt                                                                                                                                            <  2:476268/149‑1 (MQ=255)
          aCCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGAATGTCCATAttac                                                                                                                                          <  2:298480/149‑3 (MQ=255)
          aCCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGAATGTCCATAttac                                                                                                                                          <  2:379335/149‑3 (MQ=255)
           ccTTGTCAGGGGAGAAACAATGATTATTAATTAAATCTTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGAt                                                                                                                                         <  2:850474/149‑1 (MQ=255)
           ccTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGAt                                                                                                                                         >  1:1543442/1‑149 (MQ=255)
           ccTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCAATATTGAt                                                                                                                                         >  1:1091048/1‑149 (MQ=255)
           ccTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTAACAATTATCCACAAATTCCTGCGGACTAAATTAAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGAt                                                                                                                                         <  1:1031909/149‑1 (MQ=255)
             ttGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGAATGTCCATAttacatt                                                                                                                                       <  1:67490/149‑6 (MQ=255)
                      gagaAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACaaa                                                                                                                              >  1:314050/1‑149 (MQ=255)
                      gagaAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACaaa                                                                                                                              >  1:304427/1‑149 (MQ=255)
                      gagaAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACaaa                                                                                                                              >  1:528533/1‑149 (MQ=255)
                       agaAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACaaaa                                                                                                                             >  1:873414/1‑149 (MQ=255)
                       agaAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACaaaa                                                                                                                             <  1:112535/149‑1 (MQ=255)
                        gaAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAat                                                                                                                            <  2:819838/149‑1 (MQ=255)
                          aaCAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATAATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAatat                                                                                                                          >  1:954081/1‑149 (MQ=255)
                           aCAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATg                                                                                                                         <  2:110289/149‑1 (MQ=255)
                           aCAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATg                                                                                                                         >  2:1208746/1‑149 (MQ=255)
                            cAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGa                                                                                                                        <  2:960752/149‑1 (MQ=255)
                            cAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGa                                                                                                                        <  2:396404/149‑1 (MQ=255)
                            cAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGa                                                                                                                        <  2:804747/149‑1 (MQ=255)
                            cAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCAATATTGATTTGTAGAAAAAATATGa                                                                                                                        >  2:1069989/1‑149 (MQ=255)
                                  ttattaATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACAt                                                                                                                                                    >  1:155491/1‑115 (MQ=255)
                                  ttattaATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACAt                                                                                                                                                    <  2:155491/115‑1 (MQ=255)
                                  ttattaATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTAc                                                                                                                  >  1:822246/1‑149 (MQ=255)
                                  ttattaATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTAc                                                                                                                  >  1:1252528/1‑149 (MQ=255)
                                     ttaattaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTc                                                                                                               >  2:667037/1‑149 (MQ=255)
                                       aattaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCaa                                                                                                             >  2:581193/1‑149 (MQ=255)
                                       aattaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCaa                                                                                                             <  1:1171968/149‑1 (MQ=255)
                                       aattaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCaa                                                                                                             <  2:1316258/149‑1 (MQ=255)
                                        attaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAACACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAag                                                                                                            <  2:1459313/149‑1 (MQ=255)
                                         ttaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCGATATTGATTTGTCGACAAAATATGACTCTACTTCAaga                                                                                                           <  1:1470092/149‑1 (MQ=255)
                                         ttaaATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAaga                                                                                                           >  1:562322/1‑149 (MQ=255)
                                            aaTATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGAc                                                                                                        >  1:1414352/1‑149 (MQ=255)
                                              tatTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACtt                                                                                                      <  1:621278/149‑1 (MQ=255)
                                                   cTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCtt                                                                                                 <  2:659546/149‑1 (MQ=255)
                                                   cTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCtt                                                                                                 >  1:1453456/1‑149 (MQ=255)
                                                    tCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCttt                                                                                                <  2:463159/149‑1 (MQ=255)
                                                      acaATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGa                                                                                              <  1:1427473/149‑1 (MQ=255)
                                                       caATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGaa                                                                                             <  1:1239926/149‑1 (MQ=255)
                                                        aaTTATCCACAAATTCCTGCGGACTAAATTAAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGaaa                                                                                            >  2:885279/1‑149 (MQ=255)
                                                         aTTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAAc                                                                                           >  2:1535586/1‑149 (MQ=255)
                                                          ttATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACt                                                                                          >  1:852017/1‑149 (MQ=255)
                                                              ccACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggta                                                                                      <  1:425250/149‑1 (MQ=255)
                                                              ccACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggta                                                                                      <  2:925674/149‑1 (MQ=255)
                                                              ccACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggta                                                                                      <  2:895030/149‑1 (MQ=255)
                                                               cacaAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggtag                                                                                     <  1:523902/149‑1 (MQ=255)
                                                                acaAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggtagg                                                                                    >  2:1369584/1‑149 (MQ=255)
                                                                 caAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggtaggt                                                                                   >  1:1044381/1‑149 (MQ=255)
                                                                 aaaaTTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTggtaggt                                                                                   <  1:1113892/148‑1 (MQ=255)
                                                                      tCCTGCGGACTAAATTCAGCAATAAATTGATCTGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATg                                                                              <  1:43222/149‑1 (MQ=255)
                                                                        cTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGga                                                                            <  2:119139/149‑1 (MQ=255)
                                                                             gACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGAttt                                                                       >  2:621798/1‑149 (MQ=255)
                                                                                tAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTTGTAGGTATATGGAGATTTACa                                                                    >  1:1541206/1‑149 (MQ=255)
                                                                                tAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACa                                                                    >  2:1057050/1‑149 (MQ=255)
                                                                                tAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACa                                                                    >  1:148825/1‑149 (MQ=255)
                                                                                 aaaTTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAg                                                                   <  2:592138/149‑1 (MQ=255)
                                                                                  aaTTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGTTAGGTATATGGAGATTTACatt                                                                  >  1:1138422/1‑147 (MQ=255)
                                                                                        ccAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGa                                                            <  1:272390/148‑1 (MQ=255)
                                                                                          aaTAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGata                                                          <  1:1369584/149‑1 (MQ=255)
                                                                                          aaTAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGata                                                          >  2:569142/1‑149 (MQ=255)
                                                                                           aTAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGatat                                                         <  2:1312712/149‑1 (MQ=255)
                                                                                            tAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATAtt                                                        >  1:1058866/1‑149 (MQ=255)
                                                                                               aTTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGAt                                                     <  1:604826/149‑1 (MQ=255)
                                                                                                ttGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATg                                                    <  2:277454/149‑1 (MQ=255)
                                                                                                ttGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATCCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATg                                                    <  2:193322/149‑1 (MQ=255)
                                                                                                     cGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAAt                                               <  2:1414063/149‑1 (MQ=255)
                                                                                                      ggTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAAtt                                              <  1:1208746/149‑1 (MQ=255)
                                                                                                      ggTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAAtt                                              <  1:1160711/149‑1 (MQ=255)
                                                                                                      ggTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACCCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAAtt                                              >  2:962483/1‑149 (MQ=255)
                                                                                                       ttAAAAAACACAAAACTCTTATCATCATTAAATGTAATATTGACATTCCATATTGTTTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAAttt                                             <  1:476071/148‑1 (MQ=255)
                                                                                                        tAAAAAACACAAAACGCTTATCATCATGAAAAGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAAATCGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAAtttt                                            >  1:19291/1‑149 (MQ=255)
                                                                                                         aaaaaaCACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTg                                           <  2:1131323/149‑1 (MQ=255)
                                                                                                            aaaCACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCt                                                              >  1:961762/1‑127 (MQ=255)
                                                                                                            aaaCACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTa                                        <  2:338510/149‑1 (MQ=255)
                                                                                                                 caAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACACaa                                   >  2:746164/1‑149 (MQ=255)
                                                                                                                  aaaaCGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAAc                                  >  1:1196642/1‑149 (MQ=255)
                                                                                                                              atcatGAAATTTAATATGGACATTCCATATTGATTTGTCGACATAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTTGTAGGTATATGGAGATTTACAGGACCTTATATTGATGGTAATTTTGTTACCCAACTTTCAAAAAttt                      <  1:99046/149‑1 (MQ=255)
                                                                                                                              atcatGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAAttt                      <  1:1278933/149‑1 (MQ=255)
                                                                                                                              atcatGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAAttt                      >  1:732107/1‑149 (MQ=255)
                                                                                                                               tcatGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAAtttt                     <  2:1432087/149‑1 (MQ=255)
                                                                                                                                catGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAAttttt                    >  2:1599215/1‑148 (MQ=255)
                                                                                                                                 atGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGa                   >  1:1287643/1‑149 (MQ=255)
                                                                                                                                  tGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGAt                  <  1:894754/149‑1 (MQ=255)
                                                                                                                                   gAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTTATTTTGTTACCCAACTTTCAAAAATTTTGATa                 >  2:248286/1‑149 (MQ=255)
                                                                                                                                     aaTGTTATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATTTGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATaaa               <  2:1570411/149‑1 (MQ=255)
                                                                                                                                     aaTGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATaaa               >  1:174271/1‑149 (MQ=255)
                                                                                                                                      aTGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATaaaa              <  2:1453456/149‑1 (MQ=255)
                                                                                                                                      aTGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAAATGGTAGGTATATGGAGATTTACACGACCTGATATTGATGGTAATTTTGTTACACAACTATCAAAAATTTTTATaaaa              >  2:98821/1‑149 (MQ=255)
                                                                                                                                       tGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATaaaaa             >  1:629174/1‑149 (MQ=255)
                                                                                                                                       atcaaTATGGAATGTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATaaaaa             >  2:1308048/4‑149 (MQ=255)
                                                                                                                                        tcaaTATGGAATGTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAAc            >  1:695778/3‑149 (MQ=255)
                                                                                                                                          aaTATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACAt          <  2:1137521/149‑1 (MQ=255)
                                                                                                                                          aaTATGGAATGTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGGAACTGGTAGGTATATGGAGATTTACAGGACCTCAGATTGATGGTAATTTTGTTACGCAACTTTCAAAAATTTTGATAAAAACaa          >  2:1486312/1‑148 (MQ=255)
                                                                                                                                           atatGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATc         <  2:314050/149‑1 (MQ=255)
                                                                                                                                              tGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAAc      <  1:370061/149‑1 (MQ=255)
                                                                                                                                              tGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAAc      <  2:346357/149‑1 (MQ=255)
                                                                                                                                              tGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACACAACTTTCAAAAATTTTGATAAAAACATCAAc      >  2:1243156/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GATTTCGTTAACCTTGTCAGGGGAGAAACAATGATTATTAATTAAATATTGCTCACAATTATCCACAAATTCCTGCGGACTAAATTCAGCAATAAATTGATCGGTAAAAAACACAAAACGCTTATCATCATGAAATGTAATATGGACATTCCATATTGATTTGTCGACAAAATATGACTCTACTTCAAGAGACTTATCTTTGAAACTGGTAGGTATATGGAGATTTACAGGACCTGATATTGATGGTAATTTTGTTACCCAACTTTCAAAAATTTTGATAAAAACATCAACTTCT  >  NC_000913/2995410‑2995704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: