Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,058,652 G→C 6.4% intergenic (‑242/+14) serA ← / ← rpiA D‑3‑phosphoglycerate dehydrogenase/ribose 5‑phosphate isomerase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,058,6520GC 6.4% 365.2 / 13.3 109intergenic (‑242/+14)serA/rpiAD‑3‑phosphoglycerate dehydrogenase/ribose 5‑phosphate isomerase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (46/56);  new base C (2/5);  total (48/61)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

Only 100 of 109 total aligned reads displayed.

TCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAG  >  NC_000913/3058505‑3058797
                                                                                                                                                   |                                                                                                                                                 
tCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGt                                                                                                                                                  <  1:1187613/149‑1 (MQ=255)
    gATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTccc                                                                                                                                              <  2:1460142/149‑1 (MQ=255)
      tGTTGAGGAAACATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGTGGAGGTTccccc                                                                                                                                            <  1:11856/149‑1 (MQ=255)
        ttGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAACCTCCCCCGt                                                                                                                                          <  1:1349331/149‑1 (MQ=255)
         tGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAACCTCCCCCGtt                                                                                                                                         <  2:221192/149‑2 (MQ=255)
         tGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAACCTCCCCCGtt                                                                                                                                         <  2:1471840/149‑2 (MQ=255)
           aGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAg                                                                                                                                       >  1:13871/1‑149 (MQ=255)
           aGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAg                                                                                                                                       >  1:19482/1‑149 (MQ=255)
            ggtaaTATCAAGAAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAACCTCCCCCGttaaa                                                                                                                                      <  2:1161915/146‑5 (MQ=255)
            ggAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAATAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGa                                                                                                                                      <  1:576145/149‑1 (MQ=255)
             gAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTTACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGAt                                                                                                                                     >  1:879862/1‑149 (MQ=255)
             gAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCTCCGTCAGAt                                                                                                                                     >  1:716752/1‑149 (MQ=255)
             gAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGAAATAGACAATTGCCTGTTAAAAGCGTGAAACATGTCACCAAATTTAATGAAGATAATTTTTTTAACGGTGGAGGTTCCTCAGTCAGAt                                                                                                                                     >  1:710987/1‑149 (MQ=255)
             gAAATATCACGCAAAGGTAATTCTTGGATGAATATGCCGGTTTAGAGGATTTATTTAGAGCAAGCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGAt                                                                                                                                     <  1:765933/149‑1 (MQ=255)
               aaTATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAACCTCCCCCGttaaaaaa                                                                                                                                   <  2:1225783/149‑8 (MQ=255)
                 tatCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCTTCAGATCAtt                                                                                                                                 >  2:964555/1‑149 (MQ=255)
                 tatCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCAtt                                                                                                                                 <  1:715091/149‑1 (MQ=255)
                  atCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCAttt                                                                                                                                <  1:789594/149‑1 (MQ=255)
                  atCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCAttt                                                                                                                                <  2:832433/149‑1 (MQ=255)
                  atCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCAttt                                                                                                                                >  1:316375/1‑149 (MQ=255)
                  atCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAAATTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCAttt                                                                                                                                <  1:1402861/149‑1 (MQ=255)
                  atCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTAAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCAttt                                                                                                                                >  2:96851/1‑149 (MQ=255)
                   tcaagaaaaGGTAATTCTTTGATGAATATGCTGGTTTAGAGGATTTATTTATAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTc                                                                                                                               <  2:1179927/143‑1 (MQ=255)
                   tCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTc                                                                                                                               <  2:361125/149‑1 (MQ=255)
                    cACGCAAAGTTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTca                                                                                                                              <  1:1010906/149‑1 (MQ=255)
                    cACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTca                                                                                                                              >  2:749592/1‑149 (MQ=255)
                    cACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTca                                                                                                                              <  1:749593/149‑1 (MQ=255)
                    cACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTca                                                                                                                              <  1:343773/149‑1 (MQ=255)
                    cACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTca                                                                                                                              >  2:749593/1‑149 (MQ=255)
                    cACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTAATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTca                                                                                                                              <  1:196857/149‑1 (MQ=255)
                     aCGCAAAGGTAATTCTTGTATGAATATTCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTcac                                                                                                                             >  2:950854/1‑149 (MQ=255)
                     aCGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTcac                                                                                                                             <  2:415843/149‑1 (MQ=255)
                     aCGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTcac                                                                                                                             >  2:968553/1‑149 (MQ=255)
                        cAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAAt                                                                                                                          <  1:510824/149‑1 (MQ=255)
                        cAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAAt                                                                                                                          <  2:73400/149‑1 (MQ=255)
                          aaGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATgg                                                                                                                        >  2:832478/1‑149 (MQ=255)
                            ggTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGtt                                                                                                                      <  1:518767/149‑1 (MQ=255)
                              tAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGtttt                                                                                                                    <  1:541394/149‑1 (MQ=255)
                               aaTTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTg                                                                                                                   >  1:658006/1‑149 (MQ=255)
                                    ttGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACAcc                                                                                                              >  2:1147647/1‑149 (MQ=255)
                                    ttGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACAcc                                                                                                              <  2:293995/149‑1 (MQ=255)
                                     tGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCg                                                                                                             <  1:49114/149‑1 (MQ=255)
                                         tGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAg                                                                                                         <  2:458235/149‑1 (MQ=255)
                                            atatGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGgtg                                                                                                      >  1:972798/1‑149 (MQ=255)
                                             tatGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGgtgt                                                                                                     <  2:458858/149‑1 (MQ=255)
                                             tatGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGgtgt                                                                                                     <  2:1284169/149‑1 (MQ=255)
                                             tatGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGGTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGgtgt                                                                                                     <  1:64592/149‑1 (MQ=255)
                                               tGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGc                                                                                                   <  2:1221211/149‑1 (MQ=255)
                                                   ggTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAAt                                                                                               <  2:1281009/149‑1 (MQ=255)
                                                         gagGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAgcgc                                                                                         >  2:881804/1‑149 (MQ=255)
                                                           ggATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCaa                                                                                       <  1:317201/149‑1 (MQ=255)
                                                            gATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAAc                                                                                      >  1:1140692/1‑149 (MQ=255)
                                                            gATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAAc                                                                                      >  2:1400736/1‑149 (MQ=255)
                                                                       agCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCAc                                                                           <  1:755745/149‑1 (MQ=255)
                                                                       agCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCAc                                                                           >  2:768170/1‑149 (MQ=255)
                                                                         cAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACgg                                                                         <  2:5859/149‑1 (MQ=255)
                                                                            tCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTa                                                                      <  1:785253/149‑1 (MQ=255)
                                                                            tCGACAATAGCCTTGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTa                                                                      <  1:1487495/149‑1 (MQ=255)
                                                                                        tGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGAGCCACGGTTAGCAAACAAGACa                                                          >  2:485661/1‑149 (MQ=255)
                                                                                         ggTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCaa                                                         >  1:712196/1‑149 (MQ=255)
                                                                                         ggTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCaa                                                         >  1:718013/1‑149 (MQ=255)
                                                                                          gTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAAc                                                        >  2:232567/1‑149 (MQ=255)
                                                                                          gTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGACAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAAc                                                        >  1:218710/1‑149 (MQ=255)
                                                                                           tAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACa                                                       <  1:832478/149‑1 (MQ=255)
                                                                                             aaaGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGt                                                     >  2:425307/1‑149 (MQ=255)
                                                                                               aGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTca                                                   <  1:968553/149‑1 (MQ=255)
                                                                                                     acacATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCTCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGc                                             <  2:716752/149‑1 (MQ=255)
                                                                                                     acacATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGc                                             >  2:1147022/1‑149 (MQ=255)
                                                                                                     acacATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGACACCACGc                                             >  2:269695/1‑149 (MQ=255)
                                                                                                     acacATGTAACAAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCTCCGTCAGATCATTTCACAATGGTTTTGACACCGTCATGTGTGCCAATCAGCGCAACGTCCGCGCCACTGTTAGCAAACAAGCCAACAGTCACCACGc                                             <  2:710987/149‑1 (MQ=255)
                                                                                                       acaTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGAACATTTCACAATGGTTTTCACACCGTCAGGTGTGCCAAACAGCGCAACGTCCGCGCCACGGTTAGCAAACATGACAACAGTCAACACGCCa                                           >  2:637948/1‑149 (MQ=255)
                                                                                                        caTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAg                                          <  1:768170/149‑1 (MQ=255)
                                                                                                         aTGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGAAACCGGCAGTTGTGCCAAACAGCGCAACGTCCCCGCCCCGGTTAGCAAACAACCCAACAGACACCACGCCAgg                                         >  1:1576540/1‑149 (MQ=255)
                                                                                                           gTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCCGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGaa                                       >  2:647657/1‑149 (MQ=255)
                                                                                                             cACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATc                                     <  2:518981/149‑1 (MQ=255)
                                                                                                             cACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATc                                     <  2:886309/149‑1 (MQ=255)
                                                                                                               ccAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGc                                   >  1:977851/1‑149 (MQ=255)
                                                                                                               ccAAATTTAATGAAGAGAATTTTTTAAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGc                                   >  1:1400002/1‑149 (MQ=255)
                                                                                                                cAAATTTAATGATGATAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATGTCACAATGGTTTTGAGACCGTCAGGTGTGCCATTCAGCGCAACGTCCGCGCAACGGTTAGGATACAAGCCAACAGTCACAACGCCAGGAATCGCa                                  >  2:287961/1‑149 (MQ=255)
                                                                                                                cAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCa                                  >  1:885885/1‑149 (MQ=255)
                                                                                                                   aTTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCAttt                               >  2:186771/1‑149 (MQ=255)
                                                                                                                    tttAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTa                              >  1:872640/1‑149 (MQ=255)
                                                                                                                          gAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGt                        >  1:1569271/1‑149 (MQ=255)
                                                                                                                          gAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGt                        <  2:813903/149‑1 (MQ=255)
                                                                                                                             gagaATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGtttt                     >  2:432370/1‑149 (MQ=255)
                                                                                                                                aaTTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCa                  <  2:972798/149‑1 (MQ=255)
                                                                                                                                aaTTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAAGCGCATTTATGGCGTTTTCCa                  >  1:788557/1‑149 (MQ=255)
                                                                                                                                 aTTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCAt                 <  2:19482/149‑1 (MQ=255)
                                                                                                                                 aTTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAAACAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCAt                 <  2:13871/149‑1 (MQ=255)
                                                                                                                                  tttttttAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATc                >  1:1238070/1‑149 (MQ=255)
                                                                                                                                       ttAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTAt           <  2:879862/149‑1 (MQ=255)
                                                                                                                                        tAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATc          <  1:1147647/149‑1 (MQ=255)
                                                                                                                                        tAACGGGGGAGGTTCCCCAGTCAGATCATTTCACAATCGATTTGACACCGTCATGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTATCCATCGCTATc          <  1:277543/149‑1 (MQ=255)
                                                                                                                                          aCGGGGGAACCTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCgg        >  1:1309359/1‑149 (MQ=255)
                                                                                                                                             ggggAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTc     <  2:1140692/149‑1 (MQ=255)
                                                                                                                                               ggAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCaa   <  2:718013/149‑1 (MQ=255)
                                                                                                                                               ggAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCaa   <  2:712196/149‑1 (MQ=255)
                                                                                                                                                gAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCTATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAg  <  2:1527803/149‑1 (MQ=255)
                                                                                                                                                gAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAg  <  1:471566/149‑1 (MQ=255)
                                                                                                                                                gAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAg  <  1:96851/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCGCGATGTTGAGGAAATATCACGCAAAGGTAATTCTTGGATGAATATGCTGGTTTAGAGGATTTATTTAGAGCAATCGACAATTGCCTGGTAAAAGCGTGACACATGTCACCAAATTTAATGAAGAGAATTTTTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAG  >  NC_000913/3058505‑3058797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: