Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,155,230 T→G 5.6% intergenic (‑12/‑125) yqhC ← / → yqhD transcriptional activator of yqhD/aldehyde reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,155,2300TG 5.6% 376.5 / 11.9 126intergenic (‑12/‑125)yqhC/yqhDtranscriptional activator of yqhD/aldehyde reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (55/64);  new base G (3/4);  total (58/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01

Only 100 of 130 total aligned reads displayed.

GTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACC  >  NC_000913/3155083‑3155378
                                                                                                                                                   |                                                                                                                                                    
gTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCActc                                                                                                                                                     <  1:1589135/149‑1 (MQ=255)
  ccTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCTCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTg                                                                                                                                                   <  1:70181/149‑1 (MQ=255)
    tGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGga                                                                                                                                                 <  2:203885/149‑1 (MQ=255)
    tGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGga                                                                                                                                                 >  2:391801/1‑149 (MQ=255)
     gACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCAGATATTTTATTGTGATATATTTTGTATATTTTCTCCAGAAATGTGgat                                                                                                                                                >  2:325909/1‑148 (MQ=255)
       cAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGaa                                                                                                                                              <  1:1330523/149‑1 (MQ=255)
         aaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAAt                                                                                                                                            <  1:329698/149‑1 (MQ=255)
         aaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAAt                                                                                                                                            >  2:292182/1‑149 (MQ=255)
         aaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAt                                                                                                                                            <  2:1433904/149‑1 (MQ=255)
         aaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGAGGCAATTTTATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAAt                                                                                                                                            >  1:1357514/1‑149 (MQ=255)
          aCTATTTTCTTTAATTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtg                                                                                                                                           <  2:147831/149‑2 (MQ=255)
           cTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtgc                                                                                                                                          <  1:534858/149‑3 (MQ=255)
            tATTTTCTTTAGTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAgg                                                                                                                                         >  1:1507788/1‑149 (MQ=255)
                    ttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAt                                                                                                                                 >  2:657924/1‑149 (MQ=255)
                    ttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAt                                                                                                                                 >  1:1509627/1‑149 (MQ=255)
                    ttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTATTAGGAATAATGCGGCATTTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTATGTAGAAATAGGCAAGACAt                                                                                                                                 >  2:499643/1‑149 (MQ=255)
                     tATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAtt                                                                                                                                >  2:945581/1‑149 (MQ=255)
                     tATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAtt                                                                                                                                >  1:1459509/1‑149 (MQ=255)
                        ttttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGc                                                                                                                             >  1:1438068/1‑149 (MQ=255)
                         tttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCa                                                                                                                            >  2:314868/1‑149 (MQ=255)
                         tttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTATTCACGTTTCATATCGCGTAATATCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCAATCACTCTGGAGAAATAGGCAAGACATTGGCa                                                                                                                            >  1:796977/1‑149 (MQ=255)
                              gTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAAt                                                                                                                       <  1:1131639/149‑1 (MQ=255)
                                ttattaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                                                                                                     <  2:1535938/149‑1 (MQ=255)
                                ttattaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                                                                                                     <  1:1588871/149‑1 (MQ=255)
                                ttattaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                                                                                                     <  1:391801/149‑1 (MQ=255)
                                 tattaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAg                                                                                                                    >  1:234840/1‑149 (MQ=255)
                                  attaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGc                                                                                                                   >  2:975016/1‑149 (MQ=255)
                                  attaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGAAAGACATTGGCAGAAATGAGc                                                                                                                   >  1:1521680/1‑149 (MQ=255)
                                    taACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAt                                                                                                                 >  1:1457392/1‑149 (MQ=255)
                                     aaCTTTATCCGCCAGCATGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAtt                                                                                                                <  1:745451/149‑1 (MQ=255)
                                     aaCTTTATCCGCCAGCAATCGGCAAATCTCTTCACGTTTCATATCGCTTAAATTCTTAGTAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAtt                                                                                                                <  1:371540/149‑1 (MQ=255)
                                      aCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTTGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGAAAGACATTGGCAGAATTGAGCATTg                                                                                                               >  1:957251/1‑149 (MQ=255)
                                       cTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAt                                                                                                                 >  1:522263/1‑146 (MQ=255)
                                       cTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAt                                                                                                                 <  2:522263/146‑1 (MQ=255)
                                       cTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTga                                                                                                              >  2:696492/1‑149 (MQ=255)
                                        tttATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTgag                                                                                                             >  2:767517/1‑149 (MQ=255)
                                              cGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAg                                                                                                       >  2:225761/1‑149 (MQ=255)
                                                     aaGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGCAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTg                                                                                                <  1:1518434/149‑1 (MQ=255)
                                                         ggCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGa                                                                                            >  2:91716/1‑149 (MQ=255)
                                                          gaaaaTCTCTTCACGTTTCATATCGCGTAAATTCTTAGGAATAATGCGTCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGAAAGACATTTGCAGAAATGAGAATTGAGAGCCATGGCGCTGGCGAt                                                                                           <  1:653549/147‑1 (MQ=255)
                                                          gCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGAt                                                                                           >  1:288833/1‑149 (MQ=255)
                                                          gCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGAATGGGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAGTGAGAGCCAGGGCGCTGGCGAt                                                                                           <  1:866442/149‑1 (MQ=255)
                                                          gCAAATCTATTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGAt                                                                                           >  1:284468/1‑149 (MQ=255)
                                                            aaaTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATca                                                                                         <  2:738106/149‑1 (MQ=255)
                                                              aTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATcaca                                                                                       >  2:563141/1‑149 (MQ=255)
                                                              aTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCAAGGGCGCTGGCGATcaca                                                                                       >  2:137875/1‑149 (MQ=255)
                                                                   ttCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACaa                                                                                      >  1:1503487/1‑145 (MQ=255)
                                                                   ttCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaa                                                                                  >  2:474040/1‑149 (MQ=255)
                                                                   ttCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaa                                                                                  >  1:1288216/1‑149 (MQ=255)
                                                                    tCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaa                                                                                 <  2:1585601/149‑1 (MQ=255)
                                                                     cACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaaa                                                                                >  2:400877/1‑149 (MQ=255)
                                                                      aCGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCTGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaaaa                                                                               <  2:509038/149‑1 (MQ=255)
                                                                      aCGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaaaa                                                                               <  1:933714/149‑1 (MQ=255)
                                                                      aCGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaaaa                                                                               <  2:1567461/149‑1 (MQ=255)
                                                                        gTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACa                                                                             >  2:1484341/1‑149 (MQ=255)
                                                                        gTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACa                                                                             <  1:597686/149‑1 (MQ=255)
                                                                         tttCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGTCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACAt                                                                            <  2:1538432/149‑1 (MQ=255)
                                                                         tttCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACAt                                                                            <  1:988812/149‑1 (MQ=255)
                                                                         tttCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACAt                                                                            >  2:193170/1‑149 (MQ=255)
                                                                           tCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCa                                                                          >  2:372050/1‑149 (MQ=255)
                                                                           tCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCa                                                                          <  2:1564543/149‑1 (MQ=255)
                                                                            aatatCTCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATATCTCCAGCACTCTGGAGAAATAGGCAAGACATTTGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAg                                                                         <  1:44089/148‑1 (MQ=255)
                                                                              tatCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGc                                                                       <  2:1438068/149‑1 (MQ=255)
                                                                                      aaTTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGtt                                                               <  1:521763/149‑1 (MQ=255)
                                                                                      aaTTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGtt                                                               <  2:125301/149‑1 (MQ=255)
                                                                                          tCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTctct                                                           >  2:771287/1‑149 (MQ=255)
                                                                                           cTTAGGAATAATGCGGCAATTTGATTGTGCTCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTg                                                          <  2:662320/149‑1 (MQ=255)
                                                                                             tAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGcc                                                        <  2:506512/149‑1 (MQ=255)
                                                                                             tAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGcc                                                        <  1:225761/149‑1 (MQ=255)
                                                                                              aGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTATCATTTATCAAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGATAGCCAGGGAGCAGGCGATCACAATGAAAAACATCATGCAGATAGTTCTCTGcac                                                       >  2:83452/1‑147 (MQ=255)
                                                                                              aGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGccc                                                       >  2:83454/1‑149 (MQ=255)
                                                                                                gAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTc                                                     >  2:1183606/1‑149 (MQ=255)
                                                                                                 aataatGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCa                                                    >  2:1264962/1‑149 (MQ=255)
                                                                                                     atGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATAtt                                                <  2:1097189/149‑1 (MQ=255)
                                                                                                       gCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTgg                                              <  1:1085821/149‑1 (MQ=255)
                                                                                                       gCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTgg                                              <  2:1506561/149‑1 (MQ=255)
                                                                                                         ggCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGcc                                            <  2:877637/149‑1 (MQ=255)
                                                                                                           cAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCa                                          >  1:1490008/1‑149 (MQ=255)
                                                                                                            aaTTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAg                                         >  1:1225661/1‑149 (MQ=255)
                                                                                                              tttGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCa                                       >  2:551133/1‑149 (MQ=255)
                                                                                                                tGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCaaa                                     <  2:1381725/149‑1 (MQ=255)
                                                                                                                  tttGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAgg                                   <  1:678998/148‑1 (MQ=255)
                                                                                                                      tgCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGc                               <  1:372050/149‑1 (MQ=255)
                                                                                                                      tgCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGc                               >  2:1463982/1‑149 (MQ=255)
                                                                                                                        cgcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCaa                             <  1:822313/149‑1 (MQ=255)
                                                                                                                        cgcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAATGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCaa                             <  1:314868/149‑1 (MQ=255)
                                                                                                                             ttttGTATCATTTCTCCAGCAATCTGGAGAAATAGGCAAGACATTTGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAAt                        <  2:1450043/149‑1 (MQ=255)
                                                                                                                              tttGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATg                       <  2:1458781/149‑1 (MQ=255)
                                                                                                                                tggaGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATAATGCAGATCGTTATCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaa                     <  1:83452/146‑1 (MQ=255)
                                                                                                                                tGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAATCATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCTAGTAATGaa                     <  2:642476/149‑1 (MQ=255)
                                                                                                                                tGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaa                     <  2:100698/149‑1 (MQ=255)
                                                                                                                                tGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaa                     <  1:83454/149‑1 (MQ=255)
                                                                                                                                 gTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCATAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAAAATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGAAAGTAATGaac                    <  2:1357514/149‑1 (MQ=255)
                                                                                                                                    ctaTTTCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaacaac                 >  2:1250351/3‑149 (MQ=255)
                                                                                                                                     taTTTCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGGAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACt                >  1:1451477/2‑149 (MQ=255)
                                                                                                                                       tttCTCCAGCACTCTTGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACttt              <  1:1183606/149‑1 (MQ=255)
                                                                                                                                          ctcCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAAt           <  1:636655/149‑1 (MQ=255)
                                                                                                                                           tcCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATc          >  1:1473234/1‑149 (MQ=255)
                                                                                                                                              aGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGc       <  2:234840/149‑1 (MQ=255)
                                                                                                                                                   tctGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACAcc  >  1:315288/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACC  >  NC_000913/3155083‑3155378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: