Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 397,277 C→G 5.7% I213M (ATC→ATG sbmA → peptide antibiotic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913397,2770CG 5.7% 341.3 / 7.8 101I213M (ATC→ATGsbmApeptide antibiotic transporter
Reads supporting (aligned to +/- strand):  ref base C (43/52);  new base G (3/3);  total (46/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 102 total aligned reads displayed.

CTGGCGTACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGG  >  NC_000913/397133‑397423
                                                                                                                                                |                                                                                                                                                  
cTGGCGTACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGa                                                                                                                                                <  2:584782/149‑1 (MQ=255)
   gCGTACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGt                                                                                                                                             <  2:1441704/149‑1 (MQ=255)
      tACAGCGATGAACGAAAAATACATGGCTAACTGGCAACAACTGCTTCATATCGAAGGGGCCGATCAGCGTGTGCAGGAAGACACCATGCGTTTTGCTGCAACGCTGGAGAATATGGTCGTCAGTTTTATCAACGTCATGATGGCTTTGa                                                                                                                                          <  2:44042/149‑1 (MQ=255)
        cAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATc                                                                                                                                        >  2:1200173/1‑149 (MQ=255)
        cAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATc                                                                                                                                        <  2:447359/149‑1 (MQ=255)
           cGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGATGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCACCGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCCTGACGTTGATCGcc                                                                                                                                     >  1:87989/1‑149 (MQ=255)
           cGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGTCATGATGGCGTTGAtaaaa                                                                                                                                     <  2:143656/149‑5 (MQ=255)
            gATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGTCATGATGGCGTTGAtaaaac                                                                                                                                    <  2:1536049/149‑6 (MQ=255)
              tGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTc                                                                                                                                  <  1:283730/149‑1 (MQ=255)
              tGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTc                                                                                                                                  >  2:1496894/1‑149 (MQ=255)
              tGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTc                                                                                                                                  <  2:504081/149‑1 (MQ=255)
                 aCGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGCAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTg                                                                                                                               >  2:877514/1‑149 (MQ=255)
                  cGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATAGCCTTCCTGc                                                                                                                              >  1:1526982/1‑149 (MQ=255)
                   gAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGcc                                                                                                                             >  2:820787/1‑149 (MQ=255)
                    aaTATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCg                                                                                                                            >  2:909573/1‑149 (MQ=255)
                    aaTATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCg                                                                                                                            <  1:1155521/149‑1 (MQ=255)
                      tatTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCggg                                                                                                                          >  2:551961/1‑148 (MQ=255)
                      tatTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCggg                                                                                                                          >  2:557675/1‑148 (MQ=255)
                         tACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCt                                                                                                                       <  2:317599/149‑1 (MQ=255)
                          aCATGGCGTACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTg                                                                                                                      <  2:1512899/149‑1 (MQ=255)
                          aCATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTg                                                                                                                      <  1:820787/149‑1 (MQ=255)
                          aCATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTg                                                                                                                      <  2:680066/149‑1 (MQ=255)
                              ggCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTaa                                                                                                                  >  2:622397/1‑149 (MQ=255)
                              ggCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTaa                                                                                                                  >  2:1005710/1‑149 (MQ=255)
                                     ttgCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCtctc                                                                                                           >  2:396458/3‑149 (MQ=255)
                                     tGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCtctc                                                                                                           >  2:699106/1‑149 (MQ=255)
                                     tGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCtctc                                                                                                           >  2:116094/1‑149 (MQ=255)
                                       gCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCcg                                                                                                         >  2:1420251/1‑149 (MQ=255)
                                         aacaacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGCATATGGGCGTCAGTTTTATCACCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACCCTCTCcgcc                                                                                                       >  1:120976/1‑148 (MQ=255)
                                          acaacTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCgcgc                                                                                                      >  2:820481/1‑149 (MQ=255)
                                                 cGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGcc                                                                                               >  1:144085/1‑149 (MQ=255)
                                                     atatCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGTATATGGTCGTCAGTTTTATCAACTCCATCATGAGGTTGATATCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAg                                                                                           <  2:120691/149‑1 (MQ=255)
                                                     atatCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAg                                                                                           <  2:496232/149‑1 (MQ=255)
                                                     atatCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAg                                                                                           >  2:887005/1‑149 (MQ=255)
                                                       atCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCt                                                                                         >  1:139328/1‑149 (MQ=255)
                                                        tCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTg                                                                                        >  2:673268/1‑149 (MQ=255)
                                                           aaGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCg                                                                                     >  2:144702/1‑149 (MQ=255)
                                                            aGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGa                                                                                    <  1:909573/149‑1 (MQ=255)
                                                            aGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGa                                                                                    <  2:1107887/149‑1 (MQ=255)
                                                              gggCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGAtt                                                                                  <  1:1005710/149‑1 (MQ=255)
                                                              gggCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCGCTCCGCGCATGTGCCGGAGCTGCCGAtt                                                                                  <  1:166702/149‑1 (MQ=255)
                                                                gCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTAt                                                                                <  2:560625/149‑1 (MQ=255)
                                                                  cGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGGCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTTGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCg                                                                              <  1:288638/149‑1 (MQ=255)
                                                                    cacaGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCggg                                                                            <  1:1055831/149‑1 (MQ=255)
                                                                    cacaGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCggg                                                                            <  2:1352773/149‑1 (MQ=255)
                                                                    cacaGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCggg                                                                            <  2:1398447/149‑1 (MQ=255)
                                                                        gCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGcaca                                                                        >  2:50115/1‑149 (MQ=255)
                                                                           tgtgCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTc                                                                     <  1:98311/149‑1 (MQ=255)
                                                                               cAGGAAGACACCATGCGTTTTGCTTCAACGCGGGAGAATATGGGCGGCAGTTTTATCAACGCCATCATGACGGTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTa                                                                 <  1:779616/149‑1 (MQ=255)
                                                                                 ggAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACTCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATg                                                               <  2:321941/149‑1 (MQ=255)
                                                                                    aGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATTGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTc                                                            <  2:1526982/149‑1 (MQ=255)
                                                                                    aGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTc                                                            <  1:1200173/149‑1 (MQ=255)
                                                                                    aGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGGGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATgggc                                                            <  1:1200174/149‑3 (MQ=255)
                                                                                      acacCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTg                                                          >  2:331673/1‑149 (MQ=255)
                                                                                       cacCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTgg                                                         <  2:1151877/149‑1 (MQ=255)
                                                                                        acCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                        >  1:1139277/1‑149 (MQ=255)
                                                                                        acCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                        <  2:139328/149‑1 (MQ=255)
                                                                                        acCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGt                                                        <  2:791661/149‑1 (MQ=255)
                                                                                          cctGCGTCTGGCTTCCACGGTGGAGAATATCGGCGTCAGTATTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGGGCTTGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGa                                                      <  2:1036904/147‑1 (MQ=255)
                                                                                          cATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGa                                                      <  1:50115/149‑1 (MQ=255)
                                                                                          cATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGGGCTGGTAACGCGCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACAATCCGTATGGGCTGGTGa                                                      <  2:1032885/149‑1 (MQ=255)
                                                                                            tGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGAtt                                                    >  1:1041755/1‑149 (MQ=255)
                                                                                                ttttGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCg                                                <  1:877514/149‑1 (MQ=255)
                                                                                                        cAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTc                                        >  2:431636/1‑149 (MQ=255)
                                                                                                         aaCGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCt                                       <  1:116094/149‑1 (MQ=255)
                                                                                                          aCGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTg                                      >  1:1350425/1‑149 (MQ=255)
                                                                                                           cGCTGGAGAATATGGGCGTCAGTTTTATCTACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTgg                                     <  1:1496894/149‑1 (MQ=255)
                                                                                                             cTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTc                                   <  1:1420251/149‑1 (MQ=255)
                                                                                                              tGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCg                                  >  1:762270/1‑149 (MQ=255)
                                                                                                                gagaATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCt                                >  1:1417568/1‑149 (MQ=255)
                                                                                                                gagaATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCt                                >  2:468829/1‑149 (MQ=255)
                                                                                                                  gaATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGa                              >  2:690725/1‑149 (MQ=255)
                                                                                                                       tGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATgggg                         >  1:143285/1‑149 (MQ=255)
                                                                                                                        gggCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGa                        <  2:1404603/149‑1 (MQ=255)
                                                                                                                        gggCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTACTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATggggc                        >  1:856901/1‑148 (MQ=255)
                                                                                                                           cGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCg                     <  1:747054/149‑1 (MQ=255)
                                                                                                                           cGTCAGTTTTATCAACGCCATCATGACGCTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCg                     <  1:1420602/149‑1 (MQ=255)
                                                                                                                            gTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCgg                    >  1:163279/1‑149 (MQ=255)
                                                                                                                             tCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCCATCGTCTGGTCGCTGATGGGGACCGGa                   >  1:956884/1‑149 (MQ=255)
                                                                                                                             tCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGGCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGa                   <  1:820481/149‑1 (MQ=255)
                                                                                                                                 ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTAt                                                                >  1:1478919/1‑100 (MQ=255)
                                                                                                                                 ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTAt                                                                <  2:1478919/100‑1 (MQ=255)
                                                                                                                                 ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGc               >  1:638681/1‑149 (MQ=255)
                                                                                                                                 ttttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGc               <  1:144702/149‑1 (MQ=255)
                                                                                                                                  tttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt              <  1:887005/149‑1 (MQ=255)
                                                                                                                                  tttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt              <  2:1041755/149‑1 (MQ=255)
                                                                                                                                  tttATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCt              <  2:1579865/149‑1 (MQ=255)
                                                                                                                                    tATCACCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCCTGTGCCGGAGCTGCCGAGTCTCGGGCACATTCCGTCTGGGCTGGTGATTGCCGCAATCGCCTGGTCGCTTATTGGGACCGGATTGCTgg            <  1:1563990/149‑1 (MQ=255)
                                                                                                                                    tATCACCGCCAGCATGACGTGGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGCCTGGTCGCTGAGGGGGACCGGATTGCTgg            <  1:1558066/149‑1 (MQ=255)
                                                                                                                                    tATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTgg            <  2:144085/149‑1 (MQ=255)
                                                                                                                                     aTCAACGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGc           >  2:733414/1‑149 (MQ=255)
                                                                                                                                     aTCAACGTCATGATGGCGTTGATCGCCTTCCTGACGGTGCTGGTAAAGCTATCGGCGCATGTGCCGGAGATGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGGTGGc           >  2:312176/1‑149 (MQ=255)
                                                                                                                                        aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGt        <  2:1520060/149‑1 (MQ=255)
                                                                                                                                        aaCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGt        <  2:829797/149‑1 (MQ=255)
                                                                                                                                         aCGTCATGATGGCGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTg       >  1:653147/1‑149 (MQ=255)
                                                                                                                                         aCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGt                                         <  1:222787/115‑1 (MQ=255)
                                                                                                                                         aCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGt                                         >  2:222787/1‑115 (MQ=255)
                                                                                                                                         aCGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTg       >  2:654877/1‑149 (MQ=255)
                                                                                                                                         aCGCCATCATGACGTTGATCGCCTTCCTGACGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTg       >  2:1331085/1‑149 (MQ=255)
                                                                                                                                              atcatGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAgg  >  2:1593006/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CTGGCGTACAGCGATGAACGAATATTACATGGCGAACTGGCAACAACTGCGTCATATCGAAGGGGCCGCACAGCGTGTGCAGGAAGACACCATGCGTTTTGCTTCAACGCTGGAGAATATGGGCGTCAGTTTTATCAACGCCATCATGACGTTGATCGCCTTCCTGCCGGTGCTGGTAACGCTCTCCGCGCATGTGCCGGAGCTGCCGATTATCGGGCACATTCCGTATGGTCTGGTGATTGCCGCAATCGTCTGGTCGCTGATGGGGACCGGATTGCTGGCAGTGGTAGG  >  NC_000913/397133‑397423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: