Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,250,990 A→T 8.9% intergenic (+36/‑34) yqjF → / → yqjG putative quinol oxidase subunit/putative S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,250,9900AT 8.9% 256.4 / 11.8 89intergenic (+36/‑34)yqjF/yqjGputative quinol oxidase subunit/putative S‑transferase
Reads supporting (aligned to +/- strand):  ref base A (48/33);  new base T (3/5);  total (51/38)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TCAACTCGCTGATGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTGGCTCACTGCCGATGGC  >  NC_000913/3250842‑3251137
                                                                                                                                                    |                                                                                                                                                   
tCAACTCGCTGATGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAggagga                                                                                                                                                     <  1:1111075/149‑1 (MQ=255)
tCAACTCGCTGATGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAggagga                                                                                                                                                     >  1:354454/1‑149 (MQ=255)
tCAACTCGCTGATGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAggagga                                                                                                                                                     >  1:348688/1‑149 (MQ=255)
  aaCTCGCTGATGTTCATGAAACACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAg                                                                                                                                                   <  2:365022/149‑1 (MQ=255)
     tCGCTGATGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTAAAGCATCGACCGCCGGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGcta                                                                                                                                                >  2:1340737/1‑148 (MQ=255)
         tGATGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCg                                                                                                                                            >  2:606892/1‑149 (MQ=255)
          gATGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGCTTCCTCCTCGt                                                                                                                                           <  2:1261381/149‑1 (MQ=255)
          gATGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACAGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGCTTCCTCCTCGt                                                                                                                                           <  1:29091/149‑1 (MQ=255)
          ccttgtCATGAAAAACCAGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGt                                                                                                                                           <  1:109498/144‑1 (MQ=255)
           aTGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGCTTCCTCCTCGtt                                                                                                                                          <  1:1009214/149‑1 (MQ=255)
           aTGTTCATGAAAAACCTGACAATTTATGGCTGATTCCTGTTGCTGGCAATTACCGGTCCGGGCGCGTATTGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCTCGCTCTATACTTAACTTTAAATAACGAGGAGCTTCCTCCTCGtt                                                                                                                                          <  2:1073660/149‑1 (MQ=255)
             tttCATTAAAAACCTTACAATTTCTGGCGGATTCATGCTTGTGGCAATTAGCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGCTTCCTCCTCGtttt                                                                                                                                        <  2:668308/148‑1 (MQ=255)
              ttCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGttttt                                                                                                                                       >  2:1348847/1‑149 (MQ=255)
              ttCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGttttt                                                                                                                                       >  1:29693/1‑149 (MQ=255)
                cATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTACGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGATCCTCGTTTTTGc                                                                                                                                     >  1:28612/1‑149 (MQ=255)
                aaTGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGc                                                                                                                                     <  1:160835/148‑1 (MQ=255)
                   gAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCTGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGAACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTGTGCTAt                                                                                                                                  >  1:121082/1‑149 (MQ=255)
                   gAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTAt                                                                                                                                  <  2:354454/149‑1 (MQ=255)
                   gAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTAt                                                                                                                                  <  2:348688/149‑1 (MQ=255)
                   gAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTAt                                                                                                                                  <  2:953107/149‑1 (MQ=255)
                    caaaaCCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGCCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGTTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTAtt                                                                                                                                 <  1:1252494/148‑1 (MQ=255)
                    aaaaaCCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTAtt                                                                                                                                 <  1:952913/149‑1 (MQ=255)
                     aaaaCCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTg                                                                                                                                <  1:1340986/149‑1 (MQ=255)
                           tGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGgaggag                                                                                                                          >  1:1523397/1‑149 (MQ=255)
                            gACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCCTCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGgaga                                                                                                                         >  1:95822/1‑149 (MQ=255)
                            gACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGgaga                                                                                                                         >  2:1383546/1‑149 (MQ=255)
                                    cTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATg                                                                                                                 <  2:143829/149‑1 (MQ=255)
                                      ggCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCATGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATggg                                                                                                               <  1:1389204/149‑1 (MQ=255)
                                       gCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGt                                                                                                              <  2:708263/149‑1 (MQ=255)
                                           aTTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAAc                                                                                                          <  1:618343/149‑1 (MQ=255)
                                           aTTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAAc                                                                                                          <  2:1536708/149‑1 (MQ=255)
                                           aTTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAAc                                                                                                          <  1:658769/149‑1 (MQ=255)
                                           aTTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAAc                                                                                                          <  2:665190/149‑1 (MQ=255)
                                                 gctgctgGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCGTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTg                                                                                                    >  1:1432503/1‑149 (MQ=255)
                                                 gctgctgGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAAAGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTg                                                                                                    >  2:1125136/1‑149 (MQ=255)
                                                   tgctgGCAATTACAGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACCCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGAc                                                                                                  >  2:1101245/1‑149 (MQ=255)
                                                      tgGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAATCGAGGAGGAAGATCCGCGGTTTTGCTATGGGTGGAGAGTAAAATGGGTCAACTGATTGACGGc                                                                                               >  1:879266/1‑149 (MQ=255)
                                                           aTTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTg                                                                                          >  1:381957/1‑149 (MQ=255)
                                                           aTTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGGGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTg                                                                                          <  1:723073/149‑1 (MQ=255)
                                                            ttACCGGTCCGGGCGCATATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTAGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAAATGATTGACGGCGTCTgg                                                                                         >  2:579193/1‑149 (MQ=255)
                                                             tACCGGTCCGGGCGCTTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGc                                                                                        <  2:1430673/149‑1 (MQ=255)
                                                             tACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGc                                                                                        <  1:606892/149‑1 (MQ=255)
                                                                cGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATg                                                                                     >  1:174023/1‑149 (MQ=255)
                                                                cGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATg                                                                                     >  2:1230766/1‑149 (MQ=255)
                                                                        gcgcGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTgg                                                                             <  1:342586/149‑1 (MQ=255)
                                                                             tataGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGa                                                                        >  2:1102730/1‑149 (MQ=255)
                                                                                aGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATAc                                                                     <  2:1171282/149‑1 (MQ=255)
                                                                                       aCCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCt                                                              >  2:1167814/1‑149 (MQ=255)
                                                                                       aCCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCt                                                              >  1:599537/1‑149 (MQ=255)
                                                                                         cGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTAc                                                            >  2:1403478/1‑149 (MQ=255)
                                                                                         cGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTAc                                                            <  2:1338816/149‑1 (MQ=255)
                                                                                           ccTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACcg                                                          >  1:829923/1‑149 (MQ=255)
                                                                                           ccTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACcg                                                          >  1:691341/1‑149 (MQ=255)
                                                                                             tgctgAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAATCGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGAt                                                                       <  2:559603/134‑1 (MQ=255)
                                                                                             tgctgAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAATCGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGAt                                                                       >  1:559603/1‑134 (MQ=255)
                                                                                               ctgAATATAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTATTGCTATTGGAGGAGAGAAAAATGGGTCAAATGATTGACGGCGTCTGGCATGACACCTGGTACCATACAAAATCTACcggcgg                                                      >  2:803591/1‑149 (MQ=255)
                                                                                               ctgAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTTTTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACaggcgt                                                      >  2:306941/1‑148 (MQ=255)
                                                                                               ctgAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGGCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACcggcgg                                                      <  1:1348847/149‑1 (MQ=255)
                                                                                                tgAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGt                                                     >  1:1401630/1‑149 (MQ=255)
                                                                                                tgAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGt                                                     >  1:203687/1‑149 (MQ=255)
                                                                                                 gAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAAt                                                                >  1:1534693/1‑137 (MQ=255)
                                                                                                 gAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAAt                                                                <  2:1534693/137‑1 (MQ=255)
                                                                                                 gAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTa                                                    >  2:818173/1‑149 (MQ=255)
                                                                                                  aaTAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTaa                                                   >  2:1413120/1‑149 (MQ=255)
                                                                                                    tAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAAt                                                 >  2:345224/1‑149 (MQ=255)
                                                                                                       aaaGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTc                                              >  2:777207/1‑149 (MQ=255)
                                                                                                           tGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACg                                          >  2:810942/1‑149 (MQ=255)
                                                                                                             gTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGtt                                        >  2:1427471/1‑149 (MQ=255)
                                                                                                              tAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTc                                       <  2:197857/149‑1 (MQ=255)
                                                                                                               aaGCACGCGCTATACTTAACGATAAAAAACGAGGATGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGAAACCAAATCTACAGACGGTAAATTTCACCGTTCa                                      >  2:1166418/1‑149 (MQ=255)
                                                                                                                 gCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGa                                                                                    <  2:132608/101‑1 (MQ=255)
                                                                                                                 gCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGa                                                                                    >  1:132608/1‑101 (MQ=255)
                                                                                                                  cACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGCCACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCt                                   >  1:313118/1‑149 (MQ=255)
                                                                                                                  cACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCt                                   >  2:694943/1‑149 (MQ=255)
                                                                                                                    cgcgCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTc                                 >  2:234342/1‑149 (MQ=255)
                                                                                                                     gcgcTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTcc                                <  2:95822/149‑1 (MQ=255)
                                                                                                                       gcTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGc                              >  2:762480/1‑149 (MQ=255)
                                                                                                                        cTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCa                             >  1:130523/1‑149 (MQ=255)
                                                                                                                           taCTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCAttt                          <  1:1230766/149‑1 (MQ=255)
                                                                                                                                 aCGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGTTACGATACCAAATCTACCTGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAAc                    >  2:551147/1‑149 (MQ=255)
                                                                                                                                 aCGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAAc                    <  2:29693/149‑1 (MQ=255)
                                                                                                                                  atagcaaaaaCGAGGAGCTTCCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACt                   >  1:612360/6‑149 (MQ=255)
                                                                                                                                   gATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTg                  >  2:1349068/1‑149 (MQ=255)
                                                                                                                                   gATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTAAACGTTCAGCTTCCGCATTTCGTTACTg                  >  1:1591368/1‑149 (MQ=255)
                                                                                                                                    agcaaaaaCGAGGAGCTTCCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTgg                 >  1:1161579/4‑149 (MQ=255)
                                                                                                                                    aTAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCACCGTTCAGCTTCCGCATTTCGTAACtcg                 >  2:624356/1‑147 (MQ=255)
                                                                                                                                    aTAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTgg                 <  1:1101245/149‑1 (MQ=255)
                                                                                                                                         aaaCGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTGGCTCAc            >  1:1209650/1‑149 (MQ=255)
                                                                                                                                          aaCGAGGAGCTTCCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTGGCTCACt           >  2:650652/1‑149 (MQ=255)
                                                                                                                                           aCGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTGGCTCACTg          >  2:1224783/1‑149 (MQ=255)
                                                                                                                                            cGAGGAGGAAGCTCGTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATTCCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTGGCTCACTGc         <  2:121082/149‑1 (MQ=255)
                                                                                                                                             gaggagGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATTGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTGGCTCACTGcc        <  2:28612/149‑1 (MQ=255)
                                                                                                                                                gaggaAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTTAATTTCAACGTTCAGCATCCGCATTTCGTAACTGGCTCACTGCCGAt     >  1:1533368/1‑149 (MQ=255)
                                                                                                                                                   gaAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTGGCTCACTGCCGATGgc  >  2:1469593/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCAACTCGCTGATGTTCATGAAAAACCTGACAATTTCTGGCGGATTCCTGCTGCTGGCAATTACCGGTCCGGGCGCGTATAGCATCGACCGCCTGCTGAATAAAAAGTGGTAAGCACGCGCTATACTTAACGATAAAAAACGAGGAGGAAGCTCCTCGTTTTTGCTATTGGAGGAGAGAAAAATGGGTCAACTGATTGACGGCGTCTGGCATGACACCTGGTACGATACCAAATCTACCGGCGGTAAATTTCAACGTTCAGCTTCCGCATTTCGTAACTGGCTCACTGCCGATGGC  >  NC_000913/3250842‑3251137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: