Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,283,024 C→T 5.2% A350V (GCA→GTA)  agaS → tagatose‑6‑phosphate ketose/aldose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,283,0240CT 5.2% 243.9 / 2.5 77A350V (GCA→GTA) agaStagatose‑6‑phosphate ketose/aldose isomerase
Reads supporting (aligned to +/- strand):  ref base C (35/37);  new base T (2/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTA  >  NC_000913/3282877‑3283172
                                                                                                                                                   |                                                                                                                                                    
gTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCa                                                                                                                                                     <  1:501869/149‑1 (MQ=255)
gTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCa                                                                                                                                                     <  1:1296677/149‑1 (MQ=255)
 tGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCAc                                                                                                                                                    >  2:484065/1‑149 (MQ=255)
    cAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGa                                                                                                                                                 <  1:601264/149‑1 (MQ=255)
     aaCCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGAt                                                                                                                                                <  2:966509/149‑1 (MQ=255)
         aGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAg                                                                                                                                            <  2:476823/149‑1 (MQ=255)
          ggCACTGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATGGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGACCAGACGTTTGCACTGATGCAGt                                                                                                                                           <  1:1061490/149‑1 (MQ=255)
             aaggCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCCCCGTCCCGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGc                                                                                                                                        <  1:233452/146‑1 (MQ=255)
              aTGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCt                                                                                                                                       <  1:212799/149‑1 (MQ=255)
              aTGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCt                                                                                                                                       <  1:207938/149‑1 (MQ=255)
              aTGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCt                                                                                                                                       >  1:1458613/1‑149 (MQ=255)
                 cGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGca                                                                                                                                    >  2:242009/1‑149 (MQ=255)
                  gtgtAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGCTGCAGTCGCTGcac                                                                                                                                   >  1:773176/1‑149 (MQ=255)
                  gtgtAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGcac                                                                                                                                   <  1:721797/149‑1 (MQ=255)
                     tAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATg                                                                                                                                >  2:739563/1‑149 (MQ=255)
                     tAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATg                                                                                                                                >  1:508138/1‑149 (MQ=255)
                           ccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAAt                                                                                                                          <  1:470734/149‑1 (MQ=255)
                           ccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAAt                                                                                                                          >  1:1221978/1‑149 (MQ=255)
                           ccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCAGTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAAt                                                                                                                          >  1:1399153/1‑149 (MQ=255)
                            catcgccGCGGAAAGCCGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGGCACTTTATCGACGGTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGAGGCAGTCGCTGCACATGGGCAATa                                                                                                                         <  1:1086575/149‑1 (MQ=255)
                            catcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGCCGTTTGTACTGATGCAGTCGCTGCACATGGGCAATa                                                                                                                         >  1:817922/1‑149 (MQ=255)
                                 ccgcGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCg                                                                                                                    >  1:607945/1‑149 (MQ=255)
                                           cagcGACGATGTCGCTGCCGGTCAACATATCATCCTGCCACCGTCACGTCACTTTAACGCCGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGACGGATACcacaa                                                                                                          >  1:209726/1‑148 (MQ=255)
                                             gcGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCa                                                                                                        <  2:1541530/149‑1 (MQ=255)
                                                  aTCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAg                                                                                                   <  2:935645/149‑1 (MQ=255)
                                                      tcgCTGCCGGTCCACATATCATCCTGCCACAGTCACGTCACTTTATCGACGTTGAGCACGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGAACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCAACAGCCAGTGGc                                                                                               >  2:1485582/1‑149 (MQ=255)
                                                       cgCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCa                                                                                              <  1:535057/149‑1 (MQ=255)
                                                        gCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCAc                                                                                             <  1:1366269/149‑1 (MQ=255)
                                                               gTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAAc                                                                                      >  1:1312571/1‑149 (MQ=255)
                                                               gTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAAc                                                                                      <  2:146522/149‑1 (MQ=255)
                                                                  cacaTATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAc                                                                                                               >  2:649705/1‑121 (MQ=255)
                                                                  cacaTATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATAc                                                                                                               <  1:649705/121‑1 (MQ=255)
                                                                       atcatcCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGTACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCgtggt                                                                              >  1:899251/1‑149 (MQ=255)
                                                                       atcatcCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCAGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCAGCACATGGGCAATACGCCGGATACCCCATCAGCCAGAGGCACCGTTAACCGCgtggt                                                                              >  2:1067749/1‑149 (MQ=255)
                                                                        tcatcCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCgtggtg                                                                             <  1:1198909/149‑1 (MQ=255)
                                                                        tcatcCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGAACCCCAAAACGTTTGCAGTCATGCAGACCATGACCATGGGCAATACCCCGGAAACCCAATCAGCCATTGGCACCGTAACCCGCgtggtc                                                                             >  2:350396/1‑148 (MQ=255)
                                                                          atcCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCa                                                                           <  1:1563178/149‑1 (MQ=255)
                                                                             cTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAgg                                                                        >  2:598983/1‑149 (MQ=255)
                                                                              tGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCCGCCAGTGGCACCGTTAACCGCGTGGTGCAACGc                                                                       >  1:1498478/1‑149 (MQ=255)
                                                                                ccACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGt                                                                     >  1:1314841/1‑149 (MQ=255)
                                                                                 cACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTa                                                                    >  2:333274/1‑149 (MQ=255)
                                                                                  aCCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTaa                                                                   <  2:1256760/149‑1 (MQ=255)
                                                                                      tcacgtcacTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAAtcat                                                               <  2:472064/149‑1 (MQ=255)
                                                                                      tcacgtcacTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAAtcat                                                               <  1:926722/149‑1 (MQ=255)
                                                                                       cacgtcacTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAAtcatt                                                              <  2:1458613/149‑1 (MQ=255)
                                                                                          gtcacTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAAtcattcat                                                           >  1:1205474/1‑149 (MQ=255)
                                                                                              cTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGTACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGt                                                       <  2:817922/149‑1 (MQ=255)
                                                                                                ttATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTgg                                                     <  1:1283381/149‑1 (MQ=255)
                                                                                                     gccGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGCCGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTggcaggc                                                >  2:1037634/3‑149 (MQ=255)
                                                                                                        gTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATa                                             >  1:363500/1‑149 (MQ=255)
                                                                                                               aGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGAt                                      >  1:76388/1‑149 (MQ=255)
                                                                                                               aGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGAt                                      >  2:593578/1‑149 (MQ=255)
                                                                                                               aGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGAt                                      <  1:338609/149‑1 (MQ=255)
                                                                                                                ggCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATc                                     <  1:548304/149‑1 (MQ=255)
                                                                                                                ggCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATc                                     <  1:1141588/149‑1 (MQ=255)
                                                                                                                 gCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCg                                    >  1:687994/1‑149 (MQ=255)
                                                                                                                   aTTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCa                                  >  2:283713/1‑149 (MQ=255)
                                                                                                                    ttttGCTTCCTGATGTACGCCCAGATGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCGATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCAt                                 >  1:245680/1‑149 (MQ=255)
                                                                                                                    ttttGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCATGCATAAGAGGATCGCAt                                 <  2:1205474/149‑1 (MQ=255)
                                                                                                                    ttttGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCAt                                 <  2:1039780/149‑1 (MQ=255)
                                                                                                                    ttttGCTTCCTGATGAACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCAt                                 <  1:57129/149‑1 (MQ=255)
                                                                                                                     tttGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCAtt                                >  2:837734/1‑149 (MQ=255)
                                                                                                                       tGCTTCCTGCTGTACGCCCCGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTACCCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTAt                              <  1:983688/149‑1 (MQ=255)
                                                                                                                         cTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGa                            <  1:431767/149‑1 (MQ=255)
                                                                                                                            ccTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCa                         >  1:512450/1‑149 (MQ=255)
                                                                                                                            ccTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCa                         >  2:1408623/1‑149 (MQ=255)
                                                                                                                              tGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCAtt                       >  1:671275/1‑149 (MQ=255)
                                                                                                                                   tACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTAtctc                  >  1:1071150/1‑149 (MQ=255)
                                                                                                                                    aCGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCTTTGCAGGCATAAGAGGATCGCATTATGAGCATAATCTcc                 >  1:615787/1‑149 (MQ=255)
                                                                                                                                    aCGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTcc                 >  1:1428932/1‑149 (MQ=255)
                                                                                                                                     cGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCa                <  2:375925/149‑1 (MQ=255)
                                                                                                                                      gCCCAGACGTTTGTACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCAc               <  2:899251/149‑1 (MQ=255)
                                                                                                                                       cccAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACt              >  2:450261/1‑149 (MQ=255)
                                                                                                                                         cAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTAtctc                  <  1:1400663/143‑1 (MQ=255)
                                                                                                                                         cAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTAtctc                  >  2:1400663/1‑143 (MQ=255)
                                                                                                                                           gACGTTTGCACTTATGCAGTCGCTGCACATGGGGAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGTCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAat          <  2:209726/149‑1 (MQ=255)
                                                                                                                                               tttGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCt      <  1:1103142/149‑1 (MQ=255)
                                                                                                                                                   cACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCAGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTa  >  2:273941/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAGTGGCACCGTTAACCGCGTGGTGCAAGGCGTAATCATTCATCCGTGGCAGGCATAAGAGGATCGCATTATGAGCATTATCTCCACTAAATATCTGTTA  >  NC_000913/3282877‑3283172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: