Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,288,032 C→A 7.7% intergenic (+22/‑58) yraH → / → yraI putative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,288,0320CA 7.7% 315.1 / 12.7 114intergenic (+22/‑58)yraH/yraIputative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base C (57/48);  new base A (2/7);  total (59/55)
Fisher's exact test for biased strand distribution p-value = 8.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 117 total aligned reads displayed.

CCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAA  >  NC_000913/3287885‑3288180
                                                                                                                                                   |                                                                                                                                                    
       aaTCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGAt                                                                                                                                              <  1:1245700/149‑1 (MQ=255)
         tCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATaa                                                                                                                                            >  1:1210811/1‑149 (MQ=255)
         tCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATaa                                                                                                                                            <  2:589680/149‑1 (MQ=255)
          ccAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGTAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAAc                                                                                                                                           <  2:1000816/149‑1 (MQ=255)
            aaTGGTATTGTCAATGTTGCCCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACACTAGCGTTAACGTAACGATGGATTACCGGTAATACGTTAGGGCGTTATATGACCTGTCTGATAAAGc                                                                                                                                         >  2:278831/1‑149 (MQ=255)
            aaTGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTATTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGc                                                                                                                                         <  2:1113841/149‑1 (MQ=255)
            aaTGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTTTCAGATAACGc                                                                                                                                         >  1:1203538/1‑149 (MQ=255)
            aaTGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGc                                                                                                                                         >  1:506955/1‑149 (MQ=255)
            aaTGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGc                                                                                                                                         <  2:34599/149‑1 (MQ=255)
            aaTGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGATGGATTACCGTTAAAACGTTAGTGCGTTATCTGCCCTATCAGATAACGc                                                                                                                                         >  1:988322/1‑149 (MQ=255)
                gTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCtt                                                                                                                                     >  1:850240/1‑149 (MQ=255)
                 tATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCttt                                                                                                                                    <  1:510943/149‑1 (MQ=255)
                     gTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcct                                                                                                                                >  2:329342/1‑149 (MQ=255)
                      tCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCGTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCATATAACGCCCTTttcctt                                                                                                                               >  2:619658/1‑149 (MQ=255)
                      tCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttcctt                                                                                                                               >  2:208178/1‑149 (MQ=255)
                      tCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccat                                                                                                                               >  2:141304/1‑147 (MQ=255)
                       cAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttc                                                                                                                              <  2:1105029/149‑1 (MQ=255)
                        aaTGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttcc                                                                                                                             >  1:560387/1‑149 (MQ=255)
                        aaTGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttcc                                                                                                                             >  1:1337164/1‑149 (MQ=255)
                         aTGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTtccttcct                                                                                                                            >  1:1531275/1‑149 (MQ=255)
                                ccTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTtctc                                                                                                                     <  2:249814/149‑1 (MQ=255)
                                 cTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCg                                                                                                                    >  1:577061/1‑149 (MQ=255)
                                   cATCGCTTCCTACGTCTCACCCGTAGTTCCATCAGAAATCACCCCCTGGGAAGCACACGCTATCGTTAACGAAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGtt                                                                                                                  <  2:1029976/149‑1 (MQ=255)
                                   cATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGtt                                                                                                                  <  2:917715/149‑1 (MQ=255)
                                     tCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGt                                                                                                                <  1:208178/149‑1 (MQ=255)
                                          tactacGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAg                                                                                                           <  1:1338671/149‑1 (MQ=255)
                                           actacGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAgg                                                                                                          >  1:1600522/1‑149 (MQ=255)
                                             tacGTATCAACCGTAGTTCCATCAGAAATCACCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACTTTAGTGAGTTATCTTACCTGTCAGAAAACGCCCTTTTACTTCCTCTTGCTCGGTGTATAAGGTTg                                                                                                       >  2:1376584/1‑149 (MQ=255)
                                              acGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTg                                                                                                       <  2:659450/149‑1 (MQ=255)
                                              acGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTg                                                                                                       <  2:1396648/149‑1 (MQ=255)
                                               cGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATACCGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGa                                                                                                      >  1:151370/1‑149 (MQ=255)
                                               cGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGa                                                                                                      >  2:878945/1‑149 (MQ=255)
                                                gTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaa                                                                                                     <  1:1243691/149‑1 (MQ=255)
                                                   tCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGaaaaa                                                                                                  >  2:601813/1‑149 (MQ=255)
                                                    cAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTACCGCTGGATTACCGTTAATACGTAAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAAt                                                                                                 <  2:1188348/149‑1 (MQ=255)
                                                     aaCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATa                                                                                                >  1:1157723/1‑149 (MQ=255)
                                                       ccGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAAc                                                                                              <  2:560387/149‑1 (MQ=255)
                                                       ccGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAAc                                                                                              <  2:1337164/149‑1 (MQ=255)
                                                       acGTAGATCCATCAGAAATCACCCCATGGTAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACTTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAAc                                                                                              <  1:241740/148‑1 (MQ=255)
                                                         gTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTa                                                                                            <  1:893811/149‑1 (MQ=255)
                                                          tAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTAt                                                                                           >  1:559803/1‑149 (MQ=255)
                                                            gTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGt                                                                                         <  1:332555/149‑1 (MQ=255)
                                                            gTTCCATCAGAAATCACCCCAGGGGAAGCAGAAGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGt                                                                                         <  2:1506329/149‑1 (MQ=255)
                                                             ttCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAGCGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTc                                                                                        >  2:1337471/1‑149 (MQ=255)
                                                             ttCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTc                                                                                        >  2:1237217/1‑149 (MQ=255)
                                                             ttCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTc                                                                                        <  1:109067/149‑1 (MQ=255)
                                                             ttCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTc                                                                                        <  1:1522942/149‑1 (MQ=255)
                                                               ccATCAGAAATCACCACAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaa                                                                                      <  1:96327/149‑1 (MQ=255)
                                                                 aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaaaa                                                                                    >  2:1542704/1‑149 (MQ=255)
                                                                 aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaaaa                                                                                    >  2:162032/1‑149 (MQ=255)
                                                                 aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaaaa                                                                                    <  2:529121/149‑1 (MQ=255)
                                                                     gAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTGTTTCTAGTTGTATAAGGTTGAAAAATAAATTTGTAAAAAAGaa                                                                                >  2:1426476/1‑149 (MQ=255)
                                                                     gAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGaa                                                                                >  1:422684/1‑149 (MQ=255)
                                                                        aTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTAGCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATTACGCCCTTTTCGTTGCCCTTTCTCGTTTTATCAGGTTTAAAAATAAGTATGTCAAAACGAAcct                                                                             >  2:252020/1‑147 (MQ=255)
                                                                        aTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACAt                                                                             >  2:683748/1‑149 (MQ=255)
                                                                        aTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTAAGATAACGCCCTTTTCCTTCCTCTTACTCGTTGTATCAGGGTGAAAAATAACTATGTCAAAACGAACAt                                                                             >  2:764091/1‑149 (MQ=255)
                                                                             cccAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTcgcg                                                                        >  2:358088/1‑149 (MQ=255)
                                                                             cccAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCAGTCAGATTACGCCCTTTTCCTTCCTCTGTCTGCTTGTATCAGGTAGAAAAATAACTATGGCAAAACGAACATTcgag                                                                        >  1:66101/1‑147 (MQ=255)
                                                                               cAGGGGAAGCAGACGCAATCGTTAACGTAACGCTGGAATACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACCTTCGCGGt                                                                      >  2:858762/1‑149 (MQ=255)
                                                                                aGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTg                                                                     >  2:761027/1‑149 (MQ=255)
                                                                                 gggtAAGCAGACGCTATCGTTAACGTAACGCTGGATTACGGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGa                                                                    <  2:1210811/149‑1 (MQ=255)
                                                                                  gggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGat                                                                   <  2:345121/149‑1 (MQ=255)
                                                                                  gggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGat                                                                   <  2:766227/149‑1 (MQ=255)
                                                                                   ggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGata                                                                  >  1:829721/1‑149 (MQ=255)
                                                                                         ttaggatATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGGGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAAcc                                                            <  2:1203538/143‑1 (MQ=255)
                                                                                         aGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTaaaa                                                            >  1:1284261/1‑147 (MQ=255)
                                                                                              cTATCGTTAACGTAACGGTGGATTACCGTAAATACGTTAGGGCGTAATCTGACCTGTCAGAAAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgtt                                                       <  2:1226922/149‑1 (MQ=255)
                                                                                              cTATCGTTAACGTAACGCTTGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCGTTTCCTTCCTCTTTCTCGTTGTATCAGGGTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgtt                                                       <  2:264896/149‑1 (MQ=255)
                                                                                              cTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGCCCAGTCAGATAACGCCCTTTTCCTTCCTCGTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAAAGCACCTTCGCGGTGATATTAACCttgtt                                                       >  1:1439359/1‑149 (MQ=255)
                                                                                              atATCGTTAACGTAACGCGGGATTACCGTAAATACGTTAGGGCGTTATCTGACCTGTCTGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgtt                                                       <  1:337184/148‑1 (MQ=255)
                                                                                                aTCGTTAACGTAACGCTGTATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTtgttgt                                                     <  1:752309/149‑1 (MQ=255)
                                                                                                   gTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                                  <  1:1025497/149‑1 (MQ=255)
                                                                                                       acgtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCtt                                              <  2:15363/149‑1 (MQ=255)
                                                                                                         gtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCt                                            <  2:559803/149‑1 (MQ=255)
                                                                                                         gtaaagCTGTATTAACGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCt                                            <  2:1048251/149‑1 (MQ=255)
                                                                                                            acgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACCAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTCTa                                         >  2:1089443/1‑149 (MQ=255)
                                                                                                                tGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAAGCGTGTTGTGTAGATTCTGTATTgg                                     >  2:204225/1‑149 (MQ=255)
                                                                                                                tGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTGGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTgg                                     <  1:329342/149‑1 (MQ=255)
                                                                                                                 ggATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGc                                    <  1:1426476/149‑1 (MQ=255)
                                                                                                                  gATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGcc                                   <  1:1031846/149‑1 (MQ=255)
                                                                                                                  gATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGcc                                   >  2:1377220/1‑149 (MQ=255)
                                                                                                                    ttACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAg                                 <  1:447684/149‑1 (MQ=255)
                                                                                                                       ccGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                              >  1:184093/1‑149 (MQ=255)
                                                                                                                         gTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCt                            >  1:238753/1‑149 (MQ=255)
                                                                                                                         gTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCt                            >  1:547737/1‑149 (MQ=255)
                                                                                                                             aTACGTTAGGGCGTTATCTGACCTGTCAGATATCGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCa                        <  1:1337471/149‑1 (MQ=255)
                                                                                                                             aTACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCa                        <  2:1600522/149‑1 (MQ=255)
                                                                                                                              tACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTGTGTATTGTCCAGGCGCTTGGag                       >  1:655343/1‑149 (MQ=255)
                                                                                                                              tACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCag                       >  1:208363/1‑149 (MQ=255)
                                                                                                                                gaaaaGGGCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCagga                     <  2:1199226/145‑1 (MQ=255)
                                                                                                                                     gggCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAAt                >  1:14139/1‑149 (MQ=255)
                                                                                                                                         gTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGtt            >  2:8903/1‑149 (MQ=255)
                                                                                                                                          ttATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAATAATAACTATGTCAAAACGACCATTCGCGGTGATATTAACGTTGTTGTGTAGCTTCTGTATTGGACAGGCGCTTGCAGGAGGAATCGttt           >  2:833578/1‑149 (MQ=255)
                                                                                                                                              gtGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATAATAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACa       <  2:76864/148‑1 (MQ=255)
                                                                                                                                              cTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACa       <  2:506955/149‑1 (MQ=255)
                                                                                                                                              cTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACa       <  2:418274/149‑1 (MQ=255)
                                                                                                                                                 aCCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCg    >  1:316385/1‑149 (MQ=255)
                                                                                                                                                   cTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGaa  <  1:663570/149‑1 (MQ=255)
                                                                                                                                                   cTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGaa  >  1:739783/1‑149 (MQ=255)
                                                                                                                                                   cTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGaa  <  1:775949/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAA  >  NC_000913/3287885‑3288180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: