Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,313,765 A→T 6.9% A750A (GCT→GCA infB ← translation initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,313,7650AT 6.9% 285.5 / 7.4 96A750A (GCT→GCAinfBtranslation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base A (44/45);  new base T (4/3);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCAGACCGATAATCTGCTGTTTCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAGGAGTCGGAGATCGCTTCGA  >  NC_000913/3313621‑3313913
                                                                                                                                                |                                                                                                                                                    
cGCCAGACCGATAATCTGCTGTTTCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCGTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCac                                                                                                                                                  <  1:999204/149‑1 (MQ=255)
      aCCGATAATCTGCTGTTTCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCacgtacgt                                                                                                                                            <  1:948346/149‑1 (MQ=255)
          aTAATCTGCTGTTTCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCACTCATTTTATAGATGACGTAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTaaa                                                                                                                                        <  1:416116/149‑1 (MQ=255)
          aTAATCTGCTGTTTCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTaaa                                                                                                                                        >  2:867509/1‑149 (MQ=255)
          aTAATCTGCTGTTTCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTAACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCTGATGCCTCTGCacgtaaagtgat                                                                                                                                        <  2:256272/149‑8 (MQ=255)
                     tttCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGa                                                                                                                             >  2:881728/1‑149 (MQ=255)
                     tttCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGa                                                                                                                             >  2:498718/1‑149 (MQ=255)
                     tttCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGACAACCAGGa                                                                                                                             >  1:111480/1‑149 (MQ=255)
                     tttCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGNTAAAGCCAACCAGGa                                                                                                                             <  1:505058/149‑1 (MQ=255)
                      ttCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGAt                                                                                                                            <  2:709358/149‑1 (MQ=255)
                       tCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTGCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATg                                                                                                                           >  1:246720/1‑149 (MQ=255)
                       tCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAAGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATg                                                                                                                           <  1:1258248/149‑1 (MQ=255)
                         aGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAAACAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGc                                                                                                                         >  1:77965/1‑149 (MQ=255)
                           ttCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGt                                                                                                                       <  1:1116373/149‑1 (MQ=255)
                             ccGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTg                                                                                                                     <  2:993238/149‑1 (MQ=255)
                              cGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCTGATGCCTCTGCacgt                                                                                                                                                >  2:1548729/1‑121 (MQ=255)
                              cGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCTGATGCCTCTGCacgt                                                                                                                                                <  1:1548729/121‑1 (MQ=255)
                                gagaCAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGAc                                                                                                                  >  2:557251/1‑149 (MQ=255)
                                gagaCAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGAc                                                                                                                  >  1:444450/1‑149 (MQ=255)
                                  gaCAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGAcgc                                                                                                                <  1:869785/149‑1 (MQ=255)
                                          aCCGCTCATCGCCGCTTTAACTTAGTAAATCAGGTTTTAGATGAAGGATTAGTAACGCAGATCAAGTCTTTCCGCTTCAATCACTTTACGTGCAGATGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCa                                                                                                        <  2:978115/149‑1 (MQ=255)
                                           ccGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAg                                                                                                       >  2:1132878/1‑149 (MQ=255)
                                           ccGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAg                                                                                                       >  2:1582121/1‑149 (MQ=255)
                                            cGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAgg                                                                                                      >  1:1123068/1‑149 (MQ=255)
                                             gCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAggg                                                                                                     >  2:416583/1‑149 (MQ=255)
                                                 aTCGCCGCTTTCCCTTCTTCAATCAGGTTATAGATGACGGAGTCGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGc                                                                                                 <  1:739571/149‑1 (MQ=255)
                                                 aTCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGc                                                                                                 >  2:1123157/1‑149 (MQ=255)
                                                  tCGCCGCTTTCCCTTCGTCAATGAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTTAAGCCCACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCg                                                                                                >  2:256918/1‑149 (MQ=255)
                                                    gccgcTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTc                                                                                              >  1:56109/1‑149 (MQ=255)
                                                     ccgcTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCg                                                                                             >  2:708079/1‑149 (MQ=255)
                                                       gcTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGt                                                                                           <  1:514648/149‑1 (MQ=255)
                                                         tttCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGttt                                                                                         >  2:514204/1‑149 (MQ=255)
                                                            cACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCgg                                                                                      <  1:745630/149‑1 (MQ=255)
                                                            cACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCgg                                                                                      <  1:752105/149‑1 (MQ=255)
                                                             aCTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGAAGCCGCAGCCAGGGTGGCGTCGGTTTCGGt                                                                                     >  1:933167/1‑149 (MQ=255)
                                                              cTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTg                                                                                    >  2:412509/1‑149 (MQ=255)
                                                               ttCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCTTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGa                                                                                   >  2:163578/1‑149 (MQ=255)
                                                               ttCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGa                                                                                   <  1:557251/149‑1 (MQ=255)
                                                               ttCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGa                                                                                   >  1:786913/1‑149 (MQ=255)
                                                                 cGTCAATCAGGTTATAGATGACGGCGTAGTAACGCAGATCCCGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATa                                                                                 <  2:1010545/149‑1 (MQ=255)
                                                                  gTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCCGCCAGGGTGGCGTCGGTTTCGGTGATac                                                                                >  2:657287/1‑149 (MQ=255)
                                                                  gTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATac                                                                                >  2:78680/1‑149 (MQ=255)
                                                                     aaTCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccac                                                                             >  2:1456164/1‑149 (MQ=255)
                                                                     aaTCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccac                                                                             <  2:1192365/149‑1 (MQ=255)
                                                                     aaTCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATaccac                                                                             >  2:1074784/1‑149 (MQ=255)
                                                                        cAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCATCCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTa                                                                          <  2:643523/149‑1 (MQ=255)
                                                                         aGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTAc                                                                         <  1:446962/149‑1 (MQ=255)
                                                                           gTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGc                                                                       <  1:346277/149‑1 (MQ=255)
                                                                             tataGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCa                                                                     >  2:1131816/1‑149 (MQ=255)
                                                                              ataGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAg                                                                    >  2:905712/1‑149 (MQ=255)
                                                                                    ttcGGAGTAGTAAGGCAGATCGAGTCTTTCCTCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCga                                                              <  1:964928/147‑1 (MQ=255)
                                                                                      cGGAGTAGTAAGGCTGTTGCAGTCTTTCCGCTTCAATCACTTTCCGTGCAGAGGCATCTGCAGGTAGGTTATAGCCAACCAGGATGGAGTTGGACGCCTCAGCCAGGGTGGGGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatg                                                            <  2:256429/149‑1 (MQ=255)
                                                                                       ggAGTAGTAACGCAGATCCAGGCTTTCCGCGTCAATCACTTTACGTGCAGAGGCATGAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCgatga                                                           <  2:296201/149‑1 (MQ=255)
                                                                                         tgtagtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTCCGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGAGGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATc                                                         <  1:1272554/148‑1 (MQ=255)
                                                                                             gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCa                                                     >  1:431171/1‑149 (MQ=255)
                                                                                             gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCa                                                     <  1:729575/149‑1 (MQ=255)
                                                                                             gtaACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCa                                                     >  2:957516/1‑149 (MQ=255)
                                                                                              taACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCAc                                                    >  1:737852/1‑149 (MQ=255)
                                                                                                   cAGATCCAGGCTTTCCGCTTCAATCACTTTACGGGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTaa                                               <  1:1131220/149‑1 (MQ=255)
                                                                                                      aTCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGGGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACtt                                            <  1:297017/149‑1 (MQ=255)
                                                                                                       tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTc                                           <  1:1123157/149‑1 (MQ=255)
                                                                                                       tCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGGTAATGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTc                                           <  1:1150666/149‑1 (MQ=255)
                                                                                                         cAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGt                                         <  2:157378/149‑1 (MQ=255)
                                                                                                          aGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTc                                        >  2:865637/1‑149 (MQ=255)
                                                                                                          aGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATAAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTc                                        >  2:954318/1‑149 (MQ=255)
                                                                                                           ggCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCa                                       <  2:558215/149‑1 (MQ=255)
                                                                                                            gCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAg                                      <  2:246720/149‑1 (MQ=255)
                                                                                                            gCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAg                                      >  1:726116/1‑149 (MQ=255)
                                                                                                              tttCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGTCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCAGATACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTa                                    <  2:77965/149‑1 (MQ=255)
                                                                                                              tttCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCTGTGATACCACCTACGCCTGAACCGATGATCTTCACTTTAACTTCGTCAGTa                                    <  2:1220632/149‑1 (MQ=255)
                                                                                                              tttCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTa                                    <  2:933167/149‑1 (MQ=255)
                                                                                                                tCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGCCGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGa                                  >  1:1007570/1‑149 (MQ=255)
                                                                                                                tCCGCTTCAATCACTTTACGTGCAGAGGCAGCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGa                                  <  2:1027011/149‑1 (MQ=255)
                                                                                                                  cGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACa                                <  1:514204/149‑1 (MQ=255)
                                                                                                                  cGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACa                                <  1:498718/149‑1 (MQ=255)
                                                                                                                   gCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATTCCACCTACGCCAGAACCGAGGATCTTCAGGTTAACTTCGGCAGTAgcgag                               >  2:1058139/1‑145 (MQ=255)
                                                                                                                   gCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAg                               >  1:1530053/1‑149 (MQ=255)
                                                                                                                     ttCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGtt                             >  2:587086/1‑149 (MQ=255)
                                                                                                                        aaTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCATGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCa                          <  2:470275/149‑1 (MQ=255)
                                                                                                                        aaTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCa                          >  1:435951/1‑149 (MQ=255)
                                                                                                                        aaTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCa                          <  1:867509/149‑1 (MQ=255)
                                                                                                                         tttaacgtACGTGCTGATGCCTCTGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAg                         >  2:689338/8‑149 (MQ=255)
                                                                                                                         tttaacgtACGTGCTGATGCCTCTGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAg                         >  1:891162/8‑149 (MQ=255)
                                                                                                                         aTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGCACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAg                         >  2:43249/1‑149 (MQ=255)
                                                                                                                         aTCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAg                         >  2:417612/1‑149 (MQ=255)
                                                                                                                            aCTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCaa                      <  1:503054/149‑1 (MQ=255)
                                                                                                                            aCTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGAAGCAGCCAGGGTGGCGTCGGTTTCGGTGATTCCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCaa                      <  1:562542/149‑1 (MQ=255)
                                                                                                                             cTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGATCCGATGATGTTCACTTTAACTTCGTCAGTAGACAGTTTCCGCAAg                     >  1:838497/1‑149 (MQ=255)
                                                                                                                             cTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGGGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAg                     <  1:821442/149‑1 (MQ=255)
                                                                                                                              cgtACGTGCTGATGCCTCTGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAgg                    >  1:1538092/3‑149 (MQ=255)
                                                                                                                                tACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAGGAg                  <  1:78680/149‑1 (MQ=255)
                                                                                                                                           gCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAGGAGTCGGAGATCgg       >  1:355119/1‑148 (MQ=255)
                                                                                                                                           gCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAGGAGTCGGAGATCGc       >  2:370500/1‑149 (MQ=255)
                                                                                                                                              tCAGCACGTACGTTAAAGCCAACCAGGATGTCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTAGTTTATAACTGATACGCCTTAAACGTTGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAGGAGTCGGAGATCGCTTc    <  2:1123068/149‑1 (MQ=255)
                                                                                                                                              tCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAGGAGTCGGAGATCGCTTc    <  1:1456164/149‑1 (MQ=255)
                                                                                                                                               cAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAGGAGTCGGAGATCGCTTCg   >  1:1153294/1‑149 (MQ=255)
                                                                                                                                                aGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAGGAGTCGGAGATCGCTTCGa  <  1:416583/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CGCCAGACCGATAATCTGCTGTTTCAGTTCCGGAGACAGCATACCGCTCATCGCCGCTTTCACTTCGTCAATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGCAGAGGCATCAGCACGTACGTTAAAGCCAACCAGGATGGCGTTGGACGCCGCAGCCAGGGTGGCGTCGGTTTCGGTGATACCACCTACGCCAGAACCGATGATCTTCACTTTAACTTCGTCAGTAGACAGTTTCAGCAAGGAGTCGGAGATCGCTTCGA  >  NC_000913/3313621‑3313913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: