Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,322,705 C→T 5.1% intergenic (‑200/+28) secG ← / ← glmM preprotein translocase membrane subunit/phosphoglucosamine mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,322,7050CT 5.1% 314.8 / 3.8 97intergenic (‑200/+28)secG/glmMpreprotein translocase membrane subunit/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base C (39/53);  new base T (3/2);  total (42/55)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 105 total aligned reads displayed.

GCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGT  >  NC_000913/3322558‑3322853
                                                                                                                                                   |                                                                                                                                                    
gCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATcgcc                                                                                                                                                     >  1:587934/1‑149 (MQ=255)
 cGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATcgccg                                                                                                                                                    >  2:1557718/1‑149 (MQ=255)
   ggAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCgccgcc                                                                                                                                                  <  1:1524621/149‑1 (MQ=255)
   ggAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCgccgcc                                                                                                                                                  >  2:1394020/1‑149 (MQ=255)
      gTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttt                                                                                                                                               <  2:1239222/149‑1 (MQ=255)
       tttttAATCTTTCCCACTGAAGCGTGTGTGAATACTAACCAAATCGAATGACCTTCTCAAGGGCAATTTTATCGCATTGTTTCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCtttt                                                                                                                                              <  1:179308/149‑1 (MQ=255)
       tttttAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCtttt                                                                                                                                              <  2:518520/149‑1 (MQ=255)
        ttttAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGGCGATTGTCGCCttttt                                                                                                                                             <  1:718046/149‑1 (MQ=255)
         tttAATCTTTCCCATTGAAGCGGGTGTGATTACTAACCAAAGCGTATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTa                                                                                                                                            <  2:600017/149‑1 (MQ=255)
              tCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAg                                                                                                                                       <  2:456631/149‑1 (MQ=255)
                   cccACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACCGCAAAACGGAGGGTTTCGCTGAGAAAAAAGGCAACAAACGCCGCATTTTTAGCCAGTTAta                                                                                                                                  >  1:1516655/1‑148 (MQ=255)
                   cccACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAACACCGATGTGTTCGCTGAGAAAAAAGTCGACAATCGCCGCAATTTTAGCCAGATATc                                                                                                                                  >  1:81700/1‑149 (MQ=255)
                    ccACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCt                                                                                                                                 <  2:898923/149‑1 (MQ=255)
                    ccACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCt                                                                                                                                 >  1:1526646/1‑149 (MQ=255)
                    ccACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCGTTTTAGCCAGTTATCt                                                                                                                                 >  2:1588723/1‑149 (MQ=255)
                     cACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTa                                                                                                                                <  1:1429782/149‑1 (MQ=255)
                     cACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTa                                                                                                                                <  1:1580253/149‑1 (MQ=255)
                     cACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTa                                                                                                                                <  1:1211134/149‑1 (MQ=255)
                      aCTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTaa                                                                                                                               >  2:1434144/1‑149 (MQ=255)
                      aCTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTaa                                                                                                                               >  2:960699/1‑149 (MQ=255)
                           aGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCtt                                                                                                                          <  1:956966/149‑1 (MQ=255)
                                gtgtGAATACTAACCAAAGCGAATGCCCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAAc                                                                                                                     >  1:1586116/1‑149 (MQ=255)
                                gtgtGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAAc                                                                                                                     <  1:745066/149‑1 (MQ=255)
                                gtgtGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAAc                                                                                                                     <  1:1374453/149‑1 (MQ=255)
                                   tgAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGc                                                                                                                  <  1:405178/149‑1 (MQ=255)
                                   tgAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGc                                                                                                                  <  2:1012975/149‑1 (MQ=255)
                                   tgAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGc                                                                                                                  <  1:860743/149‑1 (MQ=255)
                                     aaTACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtt                                                                                                                >  2:299701/1‑149 (MQ=255)
                                     aaTACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtt                                                                                                                <  1:998316/149‑1 (MQ=255)
                                     aaTACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGACAGTTATCTAACGCTTTAAACGGCtt                                                                                                                >  1:697728/1‑149 (MQ=255)
                                      aTACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCttt                                                                                                               >  2:913051/1‑149 (MQ=255)
                                       tACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCtttt                                                                                                              >  1:1360845/1‑149 (MQ=255)
                                       tACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAGGCGACAATCGCCGCATTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTa                                                                                                             >  2:559018/1‑149 (MQ=255)
                                        aCTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTa                                                                                                             <  2:136594/149‑1 (MQ=255)
                                         cTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTAc                                                                                                            <  1:960699/149‑1 (MQ=255)
                                            aCCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTGTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGc                                                                                                         >  2:404532/1‑149 (MQ=255)
                                              cAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCAt                                                                                                       >  1:473993/1‑149 (MQ=255)
                                               aaaGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCTCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTTCATc                                                                                                      >  2:1091267/1‑149 (MQ=255)
                                                      aTGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAACCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCCTTTACTGCATCGGCGATg                                                                                               <  2:209902/149‑1 (MQ=255)
                                                      aTGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATg                                                                                               <  1:695583/149‑1 (MQ=255)
                                                       tGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGc                                                                                              <  2:897053/149‑1 (MQ=255)
                                                         aCCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCCGCGATGCgg                                                                                            >  1:1029582/1‑149 (MQ=255)
                                                          ccTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgt                                                                                           <  1:1557718/149‑1 (MQ=255)
                                                          ccTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgt                                                                                           >  2:734479/1‑149 (MQ=255)
                                                          ccTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgt                                                                                           <  2:698279/149‑1 (MQ=255)
                                                          ccTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgt                                                                                           >  1:1392714/1‑149 (MQ=255)
                                                            ttCGCAAGGGCAATTTTATCGCAGTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTAATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGgtgt                                                                                         <  1:1154620/149‑1 (MQ=255)
                                                                   gggCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGTCAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAAtt                                                                                  <  1:146428/149‑1 (MQ=255)
                                                                     gCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCttt                                                                                                                         >  2:1237734/1‑108 (MQ=255)
                                                                     gCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCttt                                                                                                                         <  1:1237734/108‑1 (MQ=255)
                                                                      cAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCat                                                                               >  2:575771/1‑148 (MQ=255)
                                                                      cAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAg                                                                               >  1:700569/1‑149 (MQ=255)
                                                                        aTTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTc                                                                             <  2:1496355/149‑1 (MQ=255)
                                                                          tttATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCAc                                                                           <  1:1434144/149‑1 (MQ=255)
                                                                          tttATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCAc                                                                           <  1:299701/149‑1 (MQ=255)
                                                                           ttATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCAcc                                                                          <  1:1467342/149‑1 (MQ=255)
                                                                                gCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTgcgc                                                                     <  2:250254/149‑1 (MQ=255)
                                                                                 cATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCt                                                                    >  1:1149844/1‑149 (MQ=255)
                                                                                   ttGTATCAACTGCGGAAAAAAACAGCAAAAACGATGTGTTGGCTGTGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTACCGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAATTTCAGTCACCTGCGCtgc                                                                  <  2:263132/149‑3 (MQ=255)
                                                                                        tCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCtt                                                             >  1:1190950/1‑149 (MQ=255)
                                                                                        tCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCtt                                                             >  1:365213/1‑149 (MQ=255)
                                                                                         cAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTc                                                            <  2:587934/149‑1 (MQ=255)
                                                                                         cAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTc                                                            <  1:1581962/149‑1 (MQ=255)
                                                                                             tGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCt                                                        <  2:1360845/149‑1 (MQ=255)
                                                                                             tGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCt                                                        >  2:355417/1‑149 (MQ=255)
                                                                                             tGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCt                                                        <  1:1394020/149‑1 (MQ=255)
                                                                                             tGCGGAAAAAAACAGCAAAACCGATGTGATCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAAAGCTTTAAACTGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACATGCGCTTCGTCTTctcat                                                        >  1:1597375/1‑145 (MQ=255)
                                                                                                ggAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTcc                                                     <  1:1349859/149‑1 (MQ=255)
                                                                                                 gAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTcca                                                    >  2:226055/1‑149 (MQ=255)
                                                                                                  aaaaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTccac                                                   >  1:1163301/1‑149 (MQ=255)
                                                                                                  aaaaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGATTCGTCTTCGCCTTccac                                                   >  1:301929/1‑149 (MQ=255)
                                                                                                   aaaaaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTGCGTCTTCGCCTTccacc                                                  <  1:202191/149‑1 (MQ=255)
                                                                                                      aaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATc                                               >  1:1533704/1‑149 (MQ=255)
                                                                                                      aaaCAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATc                                               <  1:1277625/149‑1 (MQ=255)
                                                                                                             aaaaCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGaa                                        <  1:956591/149‑1 (MQ=255)
                                                                                                              aaaCCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACGATCACGCGAAt                                       <  2:1586116/149‑1 (MQ=255)
                                                                                                                  cGATGTGTTCGCTGAGAAAAAAGGCGACAATCTCCGCCTTTTTAGCCAGTTATCTAACTCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCAACTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAAc                                   <  1:1091267/149‑1 (MQ=255)
                                                                                                                        gtTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCg                             >  2:1587931/1‑149 (MQ=255)
                                                                                                                         ttCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCg                                                                                             >  2:607734/1‑84 (MQ=255)
                                                                                                                         ttCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCg                                                                                             <  1:607734/84‑1 (MQ=255)
                                                                                                                         ttCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCgg                            >  2:487784/1‑149 (MQ=255)
                                                                                                                          tCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGt                           <  2:1392714/149‑1 (MQ=255)
                                                                                                                            gCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGc                         <  2:1526646/149‑1 (MQ=255)
                                                                                                                             cTGAGAAAAACGGCGACAATCGCCGCCTTTTTAGCCAGTTAGCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGcc                        <  1:308100/149‑1 (MQ=255)
                                                                                                                             cTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGcc                        <  1:385140/149‑1 (MQ=255)
                                                                                                                              tGAGAAAAACGGCGACAATCGCCGCCTGGTTAGCCAGTTAGCTAACGCGTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAACTTCAGTCACAGGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAGCGGGTCGGTGCCg                       >  1:795081/1‑149 (MQ=255)
                                                                                                                              tGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCg                       <  2:81700/149‑1 (MQ=255)
                                                                                                                                  taaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGAtt                   >  2:473458/2‑149 (MQ=255)
                                                                                                                                  taaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACCGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGAtt                   >  1:1016500/2‑149 (MQ=255)
                                                                                                                                  taaaaaGGCGGCGATTGTCGCCTTTTTAGCCAGTTATCGAACGCTTTAAACGGCTTTTACTGCAGCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGAtt                   >  1:582170/2‑149 (MQ=255)
                                                                                                                                  aaaaaaGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGATGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGAtt                   >  2:1138910/1‑149 (MQ=255)
                                                                                                                                   aaaaaGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGAttt                  <  2:1149844/149‑1 (MQ=255)
                                                                                                                                    aaaaGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTa                 >  2:188891/1‑149 (MQ=255)
                                                                                                                                      aaGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACg               <  2:473993/149‑1 (MQ=255)
                                                                                                                                            aCAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCaa         <  1:536147/149‑1 (MQ=255)
                                                                                                                                              aaTCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAaca       >  2:422667/1‑149 (MQ=255)
                                                                                                                                                 cgccgcCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACAcgc    <  1:487784/149‑1 (MQ=255)
                                                                                                                                                  gccgccTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACAcgcg   >  2:158692/1‑149 (MQ=255)
                                                                                                                                                  gccgccTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACAcgcg   >  2:1065188/1‑149 (MQ=255)
                                                                                                                                                   ccgccTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCCTCACGCGAATTAACGGTTAGGTGACGGATTTCCGCCGCACCCCGCGt  >  1:137373/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGGGAGTTTTTAATCTTTCCCACTGAAGCGGGTGTGAATACTAACCAAAGCGAATGACCTTCGCAAGGGCAATTTTATCGCATTGTATCAACTGCGGAAAAAAACAGCAAAACCGATGTGTTCGCTGAGAAAAAAGGCGACAATCGCCGCCTTTTTAGCCAGTTATCTAACGCTTTAAACGGCTTTTACTGCATCGGCGATGCGGTGTGCAAATTCAGTCACCTGCGCTTCGTCTTCGCCTTCCACCATCACGCGAATTAACGGTTCGGTGCCGGATTTACGCAGCAACACGCGT  >  NC_000913/3322558‑3322853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: