Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,451,410 T→A 6.9% N92I (AAT→ATT)  rplW ← 50S ribosomal subunit protein L23

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,451,4100TA 6.9% 275.5 / 6.1 95N92I (AAT→ATT) rplW50S ribosomal subunit protein L23
Reads supporting (aligned to +/- strand):  ref base T (41/47);  new base A (5/2);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCTTTGAT  >  NC_000913/3451264‑3451558
                                                                                                                                                  |                                                                                                                                                    
aGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTc                                                                                                                                                    >  1:233383/1‑149 (MQ=255)
aGCAACGGAGCAAAAGGTTTGCCCTTGGGCAGCTCAGGGTTAACCAATTTAACTACGTGGCGACGCCCCGGAGATGTCGGGTTACATTTAACAACTGCCATTGTATTTCTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTc                                                                                                                                                    >  2:828238/1‑149 (MQ=255)
 gCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGAAAACCACTTTAACTACCTGGCGACGACCCGGAGAAGTCGGTTTACATTCAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGACGCCAACGAAGTCCAGATTCt                                                                                                                                                   >  2:1135496/1‑149 (MQ=255)
   aaCGGAGCAAATGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTgg                                                                                                                                                 <  2:943449/149‑1 (MQ=255)
    aCGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGc                                                                                                                                                <  1:925299/149‑1 (MQ=255)
       gAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTACTCCGACTTACACAGCGCCGCCAACGAAGTCCAGATTCTGGCctt                                                                                                                                             >  2:929016/1‑149 (MQ=255)
          cAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGGCCAGAATCTGGActtcgt                                                                                                                                          <  2:1428237/149‑3 (MQ=255)
           aaaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCTCCGCCAACGAAGTCCAGAATCTGGCCTTCttt                                                                                                                                         >  1:1001858/1‑149 (MQ=255)
           aaaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCttt                                                                                                                                         >  1:499191/1‑149 (MQ=255)
            aaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGCTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGGCCAGAATCTGGACTTCgttg                                                                                                                                        <  1:1290511/149‑2 (MQ=255)
              aGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAg                                                                                                                                      <  1:1497484/149‑1 (MQ=255)
                 tttGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGt                                                                                                                                   >  1:439048/1‑149 (MQ=255)
                  ttGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTg                                                                                                                                  <  2:1368359/149‑1 (MQ=255)
                   tGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGa                                                                                                                                 <  2:865066/149‑1 (MQ=255)
                   tGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGa                                                                                                                                 <  1:669494/149‑1 (MQ=255)
                        ttGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTaa                                                                                                                            <  1:348621/149‑1 (MQ=255)
                         tgtgCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAg                                                                                                                           <  2:630696/149‑1 (MQ=255)
                         tgtgCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGGCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAg                                                                                                                           <  1:1131756/149‑1 (MQ=255)
                              aGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttt                                                                                                                      <  2:904515/149‑1 (MQ=255)
                              aGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttt                                                                                                                      >  2:499328/1‑149 (MQ=255)
                              aGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttt                                                                                                                      >  1:1247130/1‑149 (MQ=255)
                               gCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCttttt                                                                                                                     <  1:1032415/149‑1 (MQ=255)
                               gCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCttttt                                                                                                                     <  1:306559/149‑1 (MQ=255)
                                cTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttttt                                                                                                                    >  2:1040083/1‑149 (MQ=255)
                                cTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATGCTGGCCTTCGTTCAGGGGGACGTAAGCtttttt                                                                                                                    >  1:1071000/1‑149 (MQ=255)
                                   aGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCa                                                                                                                 <  2:1345146/149‑1 (MQ=255)
                                     ggTTTAGCAATTTTACTACGTGGCGTCGACCCGGTGTTGTCGGTTTACATTTATCAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGt                                                                                                               <  2:113533/149‑1 (MQ=255)
                                          aCCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTa                                                                                                          <  2:1335668/149‑1 (MQ=255)
                                          aCCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTa                                                                                                          <  2:1332847/149‑1 (MQ=255)
                                          aCCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTa                                                                                                          >  1:1517761/1‑149 (MQ=255)
                                           ccACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACACCTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCAGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTac                                                                                                         >  2:1083693/1‑149 (MQ=255)
                                           ccACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTac                                                                                                         >  2:365838/1‑149 (MQ=255)
                                             aCTTTAACTACGTGGCGACGACCCGGAGACGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTacga                                                                                                       >  2:606303/1‑149 (MQ=255)
                                                   aCTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCg                                                                                                 >  2:936398/1‑149 (MQ=255)
                                                    cTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGa                                                                                                >  2:731324/1‑149 (MQ=255)
                                                     tACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGccgaccgaa                                                                                               >  2:333702/1‑148 (MQ=255)
                                                         tGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGc                                                                                           >  2:577805/1‑149 (MQ=255)
                                                          ggCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTTGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCt                                                                                          <  2:800952/149‑1 (MQ=255)
                                                           gCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTg                                                                                         >  2:1182592/1‑149 (MQ=255)
                                                            cgacgaCCCGGAGATGTCGGTTTACATTTAACAACTGCAATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTTAGCTTTTTTCCAGTCGATACGACGACCGATACGCTGt                                                                                        <  2:701406/149‑1 (MQ=255)
                                                                gacCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGt                                                                                    <  2:1041507/149‑1 (MQ=255)
                                                                   ccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGCCGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGAc                                                                                 >  1:1433588/1‑149 (MQ=255)
                                                                   ccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGAc                                                                                 >  1:1218447/1‑149 (MQ=255)
                                                                   ccGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGAc                                                                                 <  2:439048/149‑1 (MQ=255)
                                                                     ggAGATGTGGGTTTACATTTATAATCTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGACGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGt                                                                               <  1:1004362/149‑1 (MQ=255)
                                                                      gagaTGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGtt                                                                              >  1:668392/1‑149 (MQ=255)
                                                                          tGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGCCTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAAc                                                                          <  1:306453/149‑1 (MQ=255)
                                                                          tGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAAc                                                                          >  1:1592755/1‑149 (MQ=255)
                                                                            tCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACtt                                                                        >  1:1045373/1‑149 (MQ=255)
                                                                             cGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGGGACGTTTAACttt                                                                       <  2:469904/149‑1 (MQ=255)
                                                                             cGGTTTACATTCAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCTGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACttt                                                                       <  1:1135496/149‑1 (MQ=255)
                                                                               gTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTcc                                                                     <  1:929589/149‑1 (MQ=255)
                                                                               gTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTcc                                                                     >  1:38959/1‑149 (MQ=255)
                                                                                tttACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTccc                                                                    <  1:1083693/149‑1 (MQ=255)
                                                                                tttACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTccc                                                                    <  1:692677/149‑1 (MQ=255)
                                                                                tttACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCACCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTccc                                                                    <  2:1327436/149‑1 (MQ=255)
                                                                                   aCATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCttt                                                                 <  2:1189391/149‑1 (MQ=255)
                                                                                     aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                               >  2:737759/1‑149 (MQ=255)
                                                                                     aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                               >  1:749553/1‑149 (MQ=255)
                                                                                     aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                               >  2:1242741/1‑149 (MQ=255)
                                                                                     aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                               <  1:493305/149‑1 (MQ=255)
                                                                                     aTTTAACAACTGCCATTGTATTACTCCTCAGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                               <  1:1185310/149‑1 (MQ=255)
                                                                                            aacTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAg                                                        <  2:959219/149‑1 (MQ=255)
                                                                                             acTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAgg                                                       <  2:1080350/149‑1 (MQ=255)
                                                                                              cTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAggg                                                      >  2:617422/1‑149 (MQ=255)
                                                                                               tGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgt                                                     <  1:1137283/149‑1 (MQ=255)
                                                                                                 ccATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTGTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgtgt                                                   >  2:526455/1‑149 (MQ=255)
                                                                                                  cATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGtt                                                  >  1:121824/1‑149 (MQ=255)
                                                                                                  cATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGtt                                                  >  1:1056364/1‑149 (MQ=255)
                                                                                                     tGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAc                                               <  2:233383/149‑1 (MQ=255)
                                                                                                       tATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcga                                             <  2:1247130/149‑1 (MQ=255)
                                                                                                        aTTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTATTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTTCCAGGGTGTTAAcgac                                            >  2:1148988/1‑149 (MQ=255)
                                                                                                               ctccGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgact                                     >  1:235029/1‑149 (MQ=255)
                                                                                                                 ccGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgacttc                                   <  2:499191/149‑1 (MQ=255)
                                                                                                                     cTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgacttcgact                               <  1:417843/149‑1 (MQ=255)
                                                                                                                      ttccTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCGTTTATCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgacttcgactt                              <  1:333702/146‑1 (MQ=255)
                                                                                                                      ttACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgacttcgactt                              <  1:406196/149‑1 (MQ=255)
                                                                                                                       tACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACgacttcgacttcgacttc                             <  2:915100/149‑1 (MQ=255)
                                                                                                                        aCTCAGCGCCGCCAACGAAGTCCAGCTTCTGGCCTTCTTTCAGGGTGAAGTAAGCTTTTTTCCCGTCGCTACGACGACCGATAAGATGTCCGTGACGTTTAACTTTCCCTTTACCTACCAGGGTGTTAAcgacttcgacttcgccttaa                            >  1:866316/1‑147 (MQ=255)
                                                                                                                          tCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGCCCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                          >  2:1456733/1‑149 (MQ=255)
                                                                                                                          tCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                          <  2:498931/149‑1 (MQ=255)
                                                                                                                          tCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                          >  2:352051/1‑149 (MQ=255)
                                                                                                                           cAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAAc                         <  1:606303/149‑1 (MQ=255)
                                                                                                                                  ctgaaagAAGGCCAGAATCTGGACTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCt                  >  1:1403678/7‑149 (MQ=255)
                                                                                                                                   ccAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTg                 >  2:422585/1‑149 (MQ=255)
                                                                                                                                   ccAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTg                 <  2:749553/149‑1 (MQ=255)
                                                                                                                                   ccAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTg                 >  1:256327/1‑149 (MQ=255)
                                                                                                                                    cAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGc                >  2:700629/1‑149 (MQ=255)
                                                                                                                                     aaCGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGCCGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGca               >  1:877704/1‑149 (MQ=255)
                                                                                                                                      aagAAGGCCAGAATCTGGACTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGcac              >  2:343361/3‑149 (MQ=255)
                                                                                                                                      aagAAGGCCAGAATCTGGACTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGcac              >  1:1584940/3‑149 (MQ=255)
                                                                                                                                       agAAGGCCAGAATCTGGACTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGcaca             >  1:1414324/2‑149 (MQ=255)
                                                                                                                                        gAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCAcag            <  2:1517761/149‑1 (MQ=255)
                                                                                                                                              cAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCtt      <  1:828238/149‑1 (MQ=255)
                                                                                                                                               aGATTCTGGCCTTCTTTCAGGGTGACGTAATCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACTGCAGCttt     <  1:929016/149‑1 (MQ=255)
                                                                                                                                                  ttCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCTTTGAt  <  2:159943/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGCAACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCTTTGAT  >  NC_000913/3451264‑3451558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: