Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,796,011 G→C 7.3% M11I (ATG→ATC)  waaC → ADP‑heptose:LPS heptosyl transferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,796,0110GC 7.3% 210.3 / 6.2 67M11I (ATG→ATC) waaCADP‑heptose:LPS heptosyl transferase I
Reads supporting (aligned to +/- strand):  ref base G (35/27);  new base C (3/2);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

ACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAC  >  NC_000913/3795863‑3796156
                                                                                                                                                    |                                                                                                                                                 
acaAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATg                                                                                                                                                   <  1:850909/149‑1 (MQ=255)
  aaaGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATggg                                                                                                                                                 <  2:1415553/149‑1 (MQ=255)
        cGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGt                                                                                                                                           >  2:835013/1‑149 (MQ=255)
            atggTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCGCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTtctc                                                                                                                                       <  2:729365/147‑1 (MQ=255)
            aaGGTGACGCTGCGTTGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTtctc                                                                                                                                       <  2:1448279/149‑1 (MQ=255)
            aaGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTtctc                                                                                                                                       >  2:107908/1‑149 (MQ=255)
            aaGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAAATCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTtctc                                                                                                                                       <  2:1242616/149‑1 (MQ=255)
             cggTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGCCCATCGACGATGttttaa                                                                                                                                      <  1:1275035/148‑5 (MQ=255)
               gTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCAt                                                                                                                                    >  1:1060521/1‑149 (MQ=255)
               gTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCAt                                                                                                                                    >  2:415021/1‑149 (MQ=255)
                  aCGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACg                                                                                                                                 <  1:1529818/149‑1 (MQ=255)
                    gCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGTAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGtt                                                                                                                               <  2:1570095/149‑1 (MQ=255)
                    gCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGtt                                                                                                                               <  1:340173/149‑1 (MQ=255)
                               ttATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGcactca                                                                                                                    >  1:1341229/1‑149 (MQ=255)
                                tATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGcactcac                                                                                                                   <  1:514122/149‑1 (MQ=255)
                                tATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGcactcac                                                                                                                   <  1:1367288/149‑1 (MQ=255)
                                    aCCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGAt                                                                                                               >  1:712630/1‑149 (MQ=255)
                                      cAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGc                                                                                                             >  2:175767/1‑149 (MQ=255)
                                       agagCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGcc                                                                                                            <  1:1035422/149‑1 (MQ=255)
                                           cTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCcagc                                                                                                        >  2:1425718/1‑149 (MQ=255)
                                             tAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTTCCCGCACTCACTGATGCCcagcag                                                                                                      <  1:107908/149‑1 (MQ=255)
                                             tAATCGACATTACTCCCCAGCGCGAACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCATGACGGATGCGGGTTTTGATCGTTAAAACATCCTCGCTGGGCGATGTTCTCCATACGTTGCACGCACTCACTGATGCCcagcag                                                                                                      >  1:1140721/1‑149 (MQ=255)
                                              aaTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTAACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACAACGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAgg                                                                                                     <  1:1000287/149‑1 (MQ=255)
                                               aTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGc                                                                                                    >  1:905933/1‑149 (MQ=255)
                                                tCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCa                                                                                                   >  2:1532097/1‑149 (MQ=255)
                                                 cGACATTACTCCCCAGCGCGTACTGGAAGAACTCAGCGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCaa                                                                                                  >  1:705387/1‑149 (MQ=255)
                                                  gACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAAt                                                                                                 >  2:746306/1‑149 (MQ=255)
                                                        aCTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAgg                                                                                           <  2:1060521/149‑1 (MQ=255)
                                                          tCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGa                                                                                         <  1:1023794/149‑1 (MQ=255)
                                                              cAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGacact                                                                                     >  2:257723/1‑145 (MQ=255)
                                                                gcgcGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGt                                                                                   <  2:864414/149‑1 (MQ=255)
                                                                      aCTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACt                                                                             >  1:489341/1‑149 (MQ=255)
                                                                          gaagcaCTCAACGCGCTATTGTGACAAGAGGAAGCCTGACGGATGCGGGTTTGGATCGTTACAACATCGTCGATGGGCGATGTACTCCATACGGTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTATGACTgggg                                                                         >  1:978034/1‑148 (MQ=255)
                                                                          gaagaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggt                                                                         >  2:1045435/1‑149 (MQ=255)
                                                                          gaagaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggt                                                                         >  2:561493/1‑149 (MQ=255)
                                                                          gaagaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggt                                                                         >  1:590369/1‑149 (MQ=255)
                                                                                cTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGga                                                                   >  2:373591/1‑149 (MQ=255)
                                                                                 tCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaa                                                                  >  2:1526140/1‑149 (MQ=255)
                                                                                 tCAACGCACTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaa                                                                  >  1:8622/1‑149 (MQ=255)
                                                                                    aCGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGGGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaagaa                                                               <  2:1333709/149‑1 (MQ=255)
                                                                                        gcTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGt                                                           <  1:1190338/149‑1 (MQ=255)
                                                                                          tATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTACATACGTTGCCCGCACTCACTCATGCCCAGCCGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTc                                                         >  2:257688/1‑149 (MQ=255)
                                                                                                 aCAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGa                                                  <  1:737532/149‑1 (MQ=255)
                                                                                                 aCAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGAAGGCAATCCCAGGGATTAAGATTGACTGGGTGGTGGAAGAAGGGTTCGCACAGa                                                  >  1:113272/1‑149 (MQ=255)
                                                                                                  cAAGAGGACGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCTGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAt                                                 <  2:155129/149‑1 (MQ=255)
                                                                                                  cAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGTTTAAGTTTGACTGGGTGGTGGAAGAAGCGATCGCCCAGAt                                                 >  2:668452/1‑149 (MQ=255)
                                                                                                      agGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttcct                                             <  1:394754/149‑1 (MQ=255)
                                                                                                        gAAGCCTGACGGATGTGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttccttc                                           >  1:496218/1‑149 (MQ=255)
                                                                                                        gAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAACTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttccttc                                           <  1:119458/149‑1 (MQ=255)
                                                                                                                         ggTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTg                          <  2:1333638/149‑1 (MQ=255)
                                                                                                                          gTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGa                         <  2:8622/149‑1 (MQ=255)
                                                                                                                              tGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGa                     <  1:835013/149‑1 (MQ=255)
                                                                                                                                aTCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGt                   >  1:1280712/1‑149 (MQ=255)
                                                                                                                                aTCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGt                   >  1:898450/1‑149 (MQ=255)
                                                                                                                                 tCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGtt                  >  1:1500597/1‑149 (MQ=255)
                                                                                                                                   gTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAt                <  2:392780/149‑1 (MQ=255)
                                                                                                                                    ttAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtt               >  2:610948/1‑149 (MQ=255)
                                                                                                                                    ttAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtt               >  2:638302/1‑149 (MQ=255)
                                                                                                                                    ttAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtt               >  2:720801/1‑149 (MQ=255)
                                                                                                                                    ttAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtt               >  2:1142020/1‑149 (MQ=255)
                                                                                                                                       gaaCATCGCCCATCGACGATGTTCTCCATACGTTGCCCGCTCTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtggt                                                                      <  1:260926/90‑1 (MQ=255)
                                                                                                                                       gaaCATCGCCCATCGACGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtggt                                                                      >  2:260926/2‑91 (MQ=255)
                                                                                                                                       gaaCATCGCCCATCGACGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACGCGGTGGTCGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCt            >  1:140265/2‑149 (MQ=255)
                                                                                                                                       aaaCATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGACGTAttgcc            >  2:287170/1‑146 (MQ=255)
                                                                                                                                       aaaCATCGGCGATGGGCGAGGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCt            <  1:1063633/149‑1 (MQ=255)
                                                                                                                                          cATCGCCCATCGACGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtgtg         >  2:872272/1‑149 (MQ=255)
                                                                                                                                            tcgtcgATGGGCGATGTTCTCCATACGGTGCCCGCACTCACTGATGCCCAGCAGGCATTCCCTGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCAGGCTGCCGTTGAGCGAGTTTTTCCTGTGGc       <  1:257688/149‑1 (MQ=255)
                                                                                                                                                 gATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAc  <  2:705387/149‑1 (MQ=255)
                                                                                                                                                 gATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAc  >  1:1172816/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAC  >  NC_000913/3795863‑3796156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑