Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,815,070 A→T 6.6% I193F (ATT→TTT) ‡ slmA → nucleoid occlusion factor, anti‑FtsZ division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,0700AT 6.6% 262.5 / 6.0 88I193F (ATT→TTT) ‡slmAnucleoid occlusion factor, anti‑FtsZ division inhibitor
Reads supporting (aligned to +/- strand):  ref base A (47/35);  new base T (3/3);  total (50/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

GAATGCGTGAGGGTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCA  >  NC_000913/3814924‑3815218
                                                                                                                                                  |                                                                                                                                                    
gaATGCGTGAGGGTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAAtt                                                                                                                                                    >  2:825495/1‑149 (MQ=255)
 aaTGCGTGAGGGTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTg                                                                                                                                                   >  2:696791/1‑149 (MQ=255)
     cGTGAGGGTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCAATTAGCGGc                                                                                                                                               <  2:145730/149‑1 (MQ=255)
       tGAGGGTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGcac                                                                                                                                             >  2:1005525/1‑149 (MQ=255)
       tGAGGGTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGcac                                                                                                                                             >  2:1376958/1‑149 (MQ=255)
        gAGGGTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCAATTAGCGGccag                                                                                                                                            <  1:1092028/149‑4 (MQ=255)
         aGGGTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAg                                                                                                                                           <  1:616516/149‑1 (MQ=255)
           ggTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGtt                                                                                                                                         <  1:894456/149‑1 (MQ=255)
            gTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTg                                                                                                                                        >  1:604453/1‑149 (MQ=255)
             tGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGc                                                                                                                                       >  2:1366980/1‑149 (MQ=255)
                  gTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTaa                                                                                                                                  >  2:1152146/1‑149 (MQ=255)
                   ttACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAat                                                                                                                                 <  2:562052/149‑1 (MQ=255)
                     acacCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAatat                                                                                                                               >  1:61927/1‑149 (MQ=255)
                       accaccGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGa                                                                                                                             >  1:1524109/1‑149 (MQ=255)
                         caccGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGGAATATGACg                                                                                                                           >  2:140849/1‑149 (MQ=255)
                          accGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGc                                                                                                                          >  2:1322122/1‑149 (MQ=255)
                              aTGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGa                                                                                                                      <  1:778134/149‑1 (MQ=255)
                              aTGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGa                                                                                                                      >  2:77688/1‑149 (MQ=255)
                              aTGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGa                                                                                                                      >  2:845967/1‑149 (MQ=255)
                                   aCCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACt                                                                                                                 <  2:913579/149‑1 (MQ=255)
                                     ccTGCGGCCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTGTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACttt                                                                                                               <  2:65745/149‑1 (MQ=255)
                                      ctgctgGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACtttt                                                                                                              >  2:1449094/1‑149 (MQ=255)
                                      ctgctgGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACtttt                                                                                                              <  2:743438/149‑1 (MQ=255)
                                          tgGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATc                                                                                                          >  2:992974/1‑149 (MQ=255)
                                            gCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCTCAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCg                                                                                                        >  1:1470486/1‑149 (MQ=255)
                                            gCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCg                                                                                                        >  2:210078/1‑149 (MQ=255)
                                                gCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGa                                                                                                    <  2:626412/149‑1 (MQ=255)
                                                gCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGa                                                                                                    <  1:1366980/149‑1 (MQ=255)
                                                 ccAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGCCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAg                                                                                                   >  2:708740/1‑149 (MQ=255)
                                                  cAGATCCTTGCCTTCTGAGAAGGTATGCTGTCACGTATTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGt                                                                                                  <  1:206599/149‑1 (MQ=255)
                                                    gATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGttt                                                                                                >  2:428584/1‑149 (MQ=255)
                                                     aTCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTc                                                                                               <  2:3344/149‑1 (MQ=255)
                                                        cTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCttt                                                                                            <  1:1589517/149‑1 (MQ=255)
                                                          ggCCGTCTGTGCCGGGAGGCTGTCACGATTTGTCCGCCGCGAAATTAAATACCGCCCGACGGATGATTTTGCCGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGGTTCTTTaa                                                                                          <  1:654547/149‑1 (MQ=255)
                                                            ccTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAAc                                                                                        <  2:1194959/149‑1 (MQ=255)
                                                            ccTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAAc                                                                                        >  1:868020/1‑149 (MQ=255)
                                                              ttCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGc                                                                                      >  1:171305/1‑149 (MQ=255)
                                                              ttCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGc                                                                                      >  2:666688/1‑149 (MQ=255)
                                                                 tgtgAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCaa                                                                                   >  2:213408/1‑149 (MQ=255)
                                                                   tgAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAAc                                                                                 >  1:1556009/1‑149 (MQ=255)
                                                                   tgAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAAc                                                                                 >  1:162275/1‑149 (MQ=255)
                                                                   tgAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAAc                                                                                 >  1:527722/1‑149 (MQ=255)
                                                                   tgAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTGTCTTTAAACGCCAAAc                                                                                 >  2:932596/1‑149 (MQ=255)
                                                                      aGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAActct                                                                              >  1:838493/1‑149 (MQ=255)
                                                                       ggTATGCTGTCACGTTTTGTCCGCAGCGAATATAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCtt                                                                             <  2:215471/149‑1 (MQ=255)
                                                                         tATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTcg                                                                           <  2:171668/149‑1 (MQ=255)
                                                                           tGCTGTCACGTTTTGTCCGCAGCGAATTTAAAGACCGCCCGACGGATGATTTTGACGACCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACTCCGGATGACTTTTCAGCCGGCGCGTTTCTTTAAACGACAAACGCGTcgag                                                                         >  1:90977/1‑147 (MQ=255)
                                                                            gCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGa                                                                        <  2:717282/149‑1 (MQ=255)
                                                                               gTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAg                                                                     <  2:61771/149‑1 (MQ=255)
                                                                                tCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAgg                                                                    >  1:774610/1‑149 (MQ=255)
                                                                                tCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACCTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAgg                                                                    <  1:1005525/149‑1 (MQ=255)
                                                                                tCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTAGCAGTAATATGACGCCGGATGCCGTTTCATCCGTCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAgg                                                                    >  2:239326/1‑149 (MQ=255)
                                                                                  aCGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGcc                                                                  >  2:1269319/1‑149 (MQ=255)
                                                                                    gTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCtt                                                                <  2:1508297/149‑1 (MQ=255)
                                                                                    gTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCtt                                                                <  2:743715/149‑1 (MQ=255)
                                                                                    gTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCtt                                                                <  1:1225499/149‑1 (MQ=255)
                                                                                          tCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAAccgc                                                          <  2:580205/149‑1 (MQ=255)
                                                                                             gCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACcgccgc                                                       <  1:428584/149‑1 (MQ=255)
                                                                                             gCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACcgccgc                                                       <  1:210078/149‑1 (MQ=255)
                                                                                              cAGCGAATTTAAATACCGCCAGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGAACAGTTGCAGTAATATGACGCCGGATGACTTTTAATCCGGCGAGTTTCTTTAAACGACAAACTCTTCGCGATAGGCCTTAACAgccgcc                                                      >  1:1388778/1‑149 (MQ=255)
                                                                                                  gAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGAt                                                  >  2:1150888/1‑149 (MQ=255)
                                                                                                    aTTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTATTCATCCGGCGAGTTTCTATAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGt                                                >  1:214214/1‑149 (MQ=255)
                                                                                                     tttAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGtt                                               >  2:846484/1‑149 (MQ=255)
                                                                                                      ttAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTGATCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTc                                              <  1:238617/149‑1 (MQ=255)
                                                                                                       tAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTcc                                             <  2:774610/149‑1 (MQ=255)
                                                                                                           tACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCa                                         >  2:658360/1‑149 (MQ=255)
                                                                                                                cccGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCAAGAAGTTCCGCCATTTcc                                    >  2:1523214/1‑149 (MQ=255)
                                                                                                                 ccGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCg                                   <  1:77688/149‑1 (MQ=255)
                                                                                                                    aCGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCt                                >  1:836964/1‑149 (MQ=255)
                                                                                                                    aCGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCt                                >  1:1045223/1‑149 (MQ=255)
                                                                                                                       gatgatTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATATCCGGATtca                             >  2:264322/1‑148 (MQ=255)
                                                                                                                         tgatTTTGACGCCCGCTGGCCGCTTATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTTAACGCCATCCTCTTCGAGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTctct                           >  1:352929/1‑149 (MQ=255)
                                                                                                                          gatTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCtt                          >  2:1256163/1‑149 (MQ=255)
                                                                                                                            ttttGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTcc                        <  1:1046085/149‑1 (MQ=255)
                                                                                                                            ttttGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTcc                        <  1:845967/149‑1 (MQ=255)
                                                                                                                                  cGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTaa                  >  1:12714/1‑149 (MQ=255)
                                                                                                                                   gCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAg                 >  1:994793/1‑149 (MQ=255)
                                                                                                                                     ccGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGCCTTCTCTTCCAGGTAAGCa               >  1:773797/1‑149 (MQ=255)
                                                                                                                                     aactgtgCCGCAATTAGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCa               >  2:1372770/7‑149 (MQ=255)
                                                                                                                                        tgtgCCGCAATTAGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCt                                <  2:546363/126‑1 (MQ=255)
                                                                                                                                        tgtgCCGCAATTAGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCt                                >  1:546363/4‑129 (MQ=255)
                                                                                                                                         tGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCa           <  1:992974/149‑1 (MQ=255)
                                                                                                                                          tgCCGCAATTAGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAg          >  1:718842/2‑149 (MQ=255)
                                                                                                                                           gCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAgg         <  2:604453/149‑1 (MQ=255)
                                                                                                                                           gCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAgg         <  2:527722/149‑1 (MQ=255)
                                                                                                                                           gCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAgg         <  2:162275/149‑1 (MQ=255)
                                                                                                                                               cTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCtt     >  2:143601/1‑149 (MQ=255)
                                                                                                                                                tAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCttt    <  1:825495/149‑1 (MQ=255)
                                                                                                                                                  aTTGCTGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCa  <  1:696791/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GAATGCGTGAGGGTGAAGGTTACACCACCGATGAAACCCTGCTGGCAAGCCAGATCCTGGCCTTCTGTGAAGGTATGCTGTCACGTTTTGTCCGCAGCGAATTTAAATACCGCCCGACGGATGATTTTGACGCCCGCTGGCCGCTAATTGCGGCACAGTTGCAGTAATATGACGCCGGATGACTTTTCATCCGGCGAGTTTCTTTAAACGCCAAACTCTTCGCGATAGGCCTTAACCGCCGCCAGATGTTCCGCCATTTCCGGCTTCTCTTCCAGGTAAGCAATCAGGTCTTTCA  >  NC_000913/3814924‑3815218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: