Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,874,048 T→G 5.1% K143Q (AAA→CAA)  dgoK ← 2‑oxo‑3‑deoxygalactonate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,874,0480TG 5.1% 314.8 / 6.7 98K143Q (AAA→CAA) dgoK2‑oxo‑3‑deoxygalactonate kinase
Reads supporting (aligned to +/- strand):  ref base T (41/52);  new base G (2/3);  total (43/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

CAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAAC  >  NC_000913/3873901‑3874192
                                                                                                                                                   |                                                                                                                                                
cAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCAttta                                                                                                                                                 <  2:749560/149‑2 (MQ=255)
 aaGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAGCCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGc                                                                                                                                                >  1:556628/1‑149 (MQ=255)
  aGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTGACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCa                                                                                                                                               >  2:1157286/1‑149 (MQ=255)
     ccAGCTGTGAAGTCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTTATTTAACAGTAACTGATGTAATTCACCGGTCATCACGGTGCGAAAATGGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATg                                                                                                                                            <  1:382369/149‑1 (MQ=255)
     ccAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATg                                                                                                                                            <  2:1331494/149‑1 (MQ=255)
      cAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATgg                                                                                                                                           <  2:1199540/149‑1 (MQ=255)
       aGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATggg                                                                                                                                          <  1:1291769/149‑1 (MQ=255)
        gCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAATTGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGt                                                                                                                                         <  1:315671/149‑1 (MQ=255)
            tgAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGtgcag                                                                                                                                     <  1:980286/149‑5 (MQ=255)
            ggAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTGCAAATGGGtgcag                                                                                                                                     <  1:975156/148‑5 (MQ=255)
                 gCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCAt                                                                                                                                <  2:952730/149‑1 (MQ=255)
                 gCATCGGCAGAGTTTTCCTGCGGCGGCAAACCAGCTCAAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCac                                                                                                                                >  1:77359/1‑148 (MQ=255)
                   aTCGGCAGAGTTTTCCTGTGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                              >  2:1179542/1‑149 (MQ=255)
                   aTCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGa                                                                                                                              >  2:1138559/1‑149 (MQ=255)
                    tCGGCAGAGTGTAGCTGCGGCGGCAAACCTGCGCCAAGCAATGAGTGATATAACAGTAACTGATGTAATGCACCGGTCATCACGGTGCGAACATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGAc                                                                                                                             <  1:262819/149‑1 (MQ=255)
                     cGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACa                                                                                                                            <  1:732618/149‑1 (MQ=255)
                         agagTTTTCCTGCGGCGGCAAGCCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATaaa                                                                                                                        <  2:556628/149‑1 (MQ=255)
                         agagTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATaaa                                                                                                                        <  2:1414144/149‑1 (MQ=255)
                         agagTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATaaa                                                                                                                        <  1:65398/149‑1 (MQ=255)
                           agTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGAGGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAaga                                                                                                                      <  1:113775/149‑1 (MQ=255)
                            gTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagag                                                                                                                     <  1:989246/149‑1 (MQ=255)
                             ttttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAACTGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagaga                                                                                                                    <  1:154399/149‑1 (MQ=255)
                             ttttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagaga                                                                                                                    >  1:47760/1‑149 (MQ=255)
                             ttttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagaga                                                                                                                    <  2:101485/149‑1 (MQ=255)
                             ttttCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagaga                                                                                                                    <  2:1292422/149‑1 (MQ=255)
                                tCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCGTGACATAAAGAGagga                                                                                                                 >  1:401295/1‑149 (MQ=255)
                                tCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGagga                                                                                                                 <  1:779976/149‑1 (MQ=255)
                                  cTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaag                                                                                                               <  1:192603/149‑1 (MQ=255)
                                  cTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaag                                                                                                               <  2:1199685/149‑1 (MQ=255)
                                  cTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGaggaag                                                                                                               >  1:49709/1‑149 (MQ=255)
                                        cggcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCa                                                                                                         <  1:1252798/149‑1 (MQ=255)
                                         ggcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCag                                                                                                        <  1:1244398/149‑1 (MQ=255)
                                         ggcAAACCTGCGCCAATCAATGAGAGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGATGATTTGCTGGCTATCGGCCTGCACACATTTGCAATGCGTTACGGGCATGACATAAAGAGAGGAAGGAGCCag                                                                                                        >  2:1209977/1‑149 (MQ=255)
                                          gcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCaga                                                                                                       <  1:1224116/149‑1 (MQ=255)
                                          gcAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCaga                                                                                                       >  2:421721/1‑149 (MQ=255)
                                            aaaCCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGc                                                                                                     <  2:692058/149‑1 (MQ=255)
                                              aCCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTc                                                                                                   <  1:661484/149‑1 (MQ=255)
                                                 tGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTcgcg                                                                                                >  1:117248/1‑149 (MQ=255)
                                                  gcgcCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTcgcgc                                                                                               >  1:188601/1‑149 (MQ=255)
                                                   cgcCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTcgcgcg                                                                                              >  2:824108/1‑149 (MQ=255)
                                                     ccAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGcc                                                                                            <  1:1015102/149‑1 (MQ=255)
                                                     ccAATCAATGAGGGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGcc                                                                                            <  2:261746/149‑1 (MQ=255)
                                                           aatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCaa                                                                                      >  1:37819/1‑149 (MQ=255)
                                                           aatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCaa                                                                                      >  2:717478/1‑149 (MQ=255)
                                                           aatGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCaa                                                                                      <  1:1021461/149‑1 (MQ=255)
                                                            atGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAAt                                                                                     <  1:910925/149‑1 (MQ=255)
                                                             tGAGGGATTTAACAGTAAATGTTGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAAtt                                                                                    <  1:732182/149‑1 (MQ=255)
                                                                 tGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTgtgt                                                                                <  2:343891/149‑1 (MQ=255)
                                                                  gATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGtt                                                                               >  2:1043567/1‑149 (MQ=255)
                                                                   aTTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGttt                                                                              >  1:1085095/1‑149 (MQ=255)
                                                                   aTTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGttt                                                                              >  2:751679/1‑149 (MQ=255)
                                                                       aaCAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGTCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTttcttc                                                                          >  2:1525466/1‑149 (MQ=255)
                                                                       aaCAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTttcttc                                                                          >  2:148148/1‑149 (MQ=255)
                                                                        aCAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcg                                                                         >  2:1241117/1‑149 (MQ=255)
                                                                         cAGTAAATGATTTAATTCACCGTTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgc                                                                        <  2:1220495/149‑1 (MQ=255)
                                                                           gTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgccg                                                                      >  1:637187/1‑149 (MQ=255)
                                                                           gTAAATGATGTAATTCACCGGGCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTcgccg                                                                      <  1:932137/149‑1 (MQ=255)
                                                                              aaTGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCgcgc                                                                   >  2:1234112/1‑149 (MQ=255)
                                                                               atgatgTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCa                                                                  >  2:270473/1‑149 (MQ=255)
                                                                                tgatgTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGAGCGCCGATCAATTGTGTATCTTCGCCGCGCAt                                                                 >  1:779268/1‑149 (MQ=255)
                                                                                   tgTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATcac                                                              >  2:193141/1‑149 (MQ=255)
                                                                                        ttCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATtgt                                                         >  1:321625/1‑149 (MQ=255)
                                                                                        ttCACCGGTCATCACGGTGCGAAAAGCGGTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATtgt                                                         <  2:300587/149‑1 (MQ=255)
                                                                                          cACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCGATCGGCCTGCACCCATTTGCAATGGGGTCCGGGCATGACATAACGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTgg                                                       <  2:49782/149‑1 (MQ=255)
                                                                                                  atcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTc                                               >  1:771199/1‑149 (MQ=255)
                                                                                                   tcaCGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCa                                              <  1:270473/149‑1 (MQ=255)
                                                                                                    caCGGTGCGAACATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGTAAGTAGCCAGATCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCAt                                             <  1:1388354/149‑1 (MQ=255)
                                                                                                        gTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgaga                                         <  1:1138559/149‑1 (MQ=255)
                                                                                                        gTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgaga                                         <  1:770544/149‑1 (MQ=255)
                                                                                                        gTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATgaga                                         <  1:181698/149‑1 (MQ=255)
                                                                                                            gAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGGTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGGTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGaca                                     >  2:103184/1‑149 (MQ=255)
                                                                                                             aaaaTCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGacac                                    >  1:660091/1‑149 (MQ=255)
                                                                                                                aTCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATa                                 >  2:658148/1‑149 (MQ=255)
                                                                                                                aTCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATa                                 <  2:304954/149‑1 (MQ=255)
                                                                                                                aTCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATa                                 >  1:842529/1‑149 (MQ=255)
                                                                                                                   gTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATga                                           <  1:1340203/136‑1 (MQ=255)
                                                                                                                   gTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATga                                           >  2:1340203/1‑136 (MQ=255)
                                                                                                                      gATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCgg                           <  2:593123/149‑1 (MQ=255)
                                                                                                                      gATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCgg                           <  2:509664/149‑1 (MQ=255)
                                                                                                                      gATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCgg                           <  2:47760/149‑1 (MQ=255)
                                                                                                                      gATATGCTGGCTATCGGCCTGCACCCATATGCAATGGGTTCCCGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATAGTGTTTATTCGCCGCGCATCACATTGAGGTTATCGTCATGAGAGACACATAATCCgg                           >  2:1257603/1‑149 (MQ=255)
                                                                                                                              ggCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATc                   <  1:148148/149‑1 (MQ=255)
                                                                                                                              ggCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATc                   >  2:622733/1‑149 (MQ=255)
                                                                                                                               gCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATcc                  >  1:1311821/1‑149 (MQ=255)
                                                                                                                               gCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATcc                  <  1:653422/149‑1 (MQ=255)
                                                                                                                                ctttCGGCCTGCACCCATTTTCAATGGGTTCCGGGCATGAAATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCATTTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGTAATAATCCa                 <  1:1209977/146‑1 (MQ=255)
                                                                                                                                 tATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAg                >  1:650038/1‑149 (MQ=255)
                                                                                                                                  aTCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGa               >  2:523060/1‑149 (MQ=255)
                                                                                                                                    cGGCCTGCACCTATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGata             <  2:1564544/149‑1 (MQ=255)
                                                                                                                                     ggCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGatat            >  2:1480138/1‑149 (MQ=255)
                                                                                                                                      gCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATAtt           >  1:1188512/1‑149 (MQ=255)
                                                                                                                                       ccggaaCCCATTGCAAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGACACACATAATCCGGGAATAATCCAGATAttt          >  1:971541/6‑148 (MQ=255)
                                                                                                                                       ccTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTg          >  1:1457949/1‑149 (MQ=255)
                                                                                                                                        cTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGt         <  2:49709/149‑1 (MQ=255)
                                                                                                                                        cTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGt         <  2:842529/149‑1 (MQ=255)
                                                                                                                                         ttcACCCATTTGCAATGGGGTCCGGGCATGCCATTAAGAGAGGAAGGAGCCAGGGCTCGCGCGCCGATCAATTGTGTTGCTTCGCCGCGCATCACATTGTGGGTTTCGTCAGGAGAGACACATAATCCGGGAATACTCCAGATATTGTc        <  2:889905/147‑1 (MQ=255)
                                                                                                                                         ggaaCCCATTGCAAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTc        >  1:1017252/4‑149 (MQ=255)
                                                                                                                                           cACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGc      <  1:512606/149‑1 (MQ=255)
                                                                                                                                               cATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAAc  <  1:1157286/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGAAGGAGCCAGAGCTCGCGCGCCGATCAATTGTGTTTCTTCGCCGCGCATCACATTGTGGTTATCGTCATGAGAGACACATAATCCGGGAATAATCCAGATATTGTCGCCAAC  >  NC_000913/3873901‑3874192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑