Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,880,374 A→T 5.9% L283Q (CTG→CAG) ‡ recF ← gap repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,880,3740AT 5.9% 295.1 / 5.8 99L283Q (CTG→CAG) ‡recFgap repair protein
Reads supporting (aligned to +/- strand):  ref base A (50/43);  new base T (4/2);  total (54/45)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

ATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAG  >  NC_000913/3880227‑3880522
                                                                                                                                                   |                                                                                                                                                    
aTCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAg                                                                                                                                                     >  1:101439/1‑149 (MQ=255)
aTCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAg                                                                                                                                                     >  1:105903/1‑149 (MQ=255)
 tCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGa                                                                                                                                                    <  2:508287/149‑1 (MQ=255)
 tCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGa                                                                                                                                                    >  2:656339/1‑149 (MQ=255)
   gcgcTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCAACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACg                                                                                                                                                  >  2:955893/1‑149 (MQ=255)
           aaaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGCCGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGCCACCGCCGCCCGCTGTCACGGGTGAGGATCTCTCCTTGCGCCAGACGTAAGgcgc                                                                                                                                          >  2:425304/1‑149 (MQ=255)
           aaaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcgc                                                                                                                                          >  1:1572422/1‑149 (MQ=255)
           aaaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcgc                                                                                                                                          >  2:221205/1‑149 (MQ=255)
              gACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCTTACGTCTGGCGcaag                                                                                                                                       <  1:1167531/149‑3 (MQ=255)
                ccTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATc                                                                                                                                     <  2:101439/149‑1 (MQ=255)
                ccTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATc                                                                                                                                     <  2:105903/149‑1 (MQ=255)
                ccTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCGTGCGCCTTACGTCTGGCGcaagga                                                                                                                                     <  1:98604/149‑5 (MQ=255)
                  tgtgATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCaa                                                                                                                                   >  2:1139516/1‑149 (MQ=255)
                    tgATTGCGTCGCTTTTAAGCGGCTAGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACa                                                                                                                                 <  2:236014/149‑1 (MQ=255)
                       ttGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCt                                                                                                                              <  1:695658/149‑1 (MQ=255)
                       ttGCGGCGCGTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCt                                                                                                                              <  1:908128/149‑1 (MQ=255)
                           gTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGCCACCGCCGCCCGCTTTCACGGGTGAGGCACTCTCCGTGCGCCAGACGTACGGCGCACCTCAGCAGCTTAAg                                                                                                                          >  1:278837/1‑149 (MQ=255)
                           gTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAg                                                                                                                          <  2:877758/149‑1 (MQ=255)
                           gTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAg                                                                                                                          >  1:698268/1‑149 (MQ=255)
                           gTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAg                                                                                                                          >  1:264306/1‑149 (MQ=255)
                            tCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGc                                                                                                                         >  1:1523363/1‑149 (MQ=255)
                            tCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGc                                                                                                                         <  2:47560/149‑1 (MQ=255)
                               cTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                                                                      <  1:862534/149‑1 (MQ=255)
                               cTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                                                                      <  1:1171833/149‑1 (MQ=255)
                                ttttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCTAAATCATCTATCAGGAAGAGCCACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACCGCGTAAGCTGcc                                                                                                                     >  2:316702/1‑149 (MQ=255)
                                          cTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATaa                                                                                                           >  1:355160/1‑149 (MQ=255)
                                           tGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAg                                                                                                          >  1:27512/1‑149 (MQ=255)
                                           tGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAg                                                                                                          >  1:454785/1‑149 (MQ=255)
                                            ggCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAgg                                                                                                         <  1:567386/149‑1 (MQ=255)
                                             gCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAg                                                                                                                                >  2:512052/1‑125 (MQ=255)
                                             gCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAg                                                                                                                                <  1:512052/125‑1 (MQ=255)
                                             gCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGt                                                                                                        <  1:793333/149‑1 (MQ=255)
                                              cAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTa                                                                                                       >  2:440101/1‑149 (MQ=255)
                                              cAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTa                                                                                                       >  2:194838/1‑149 (MQ=255)
                                               aaGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTAt                                                                                                      >  1:630465/1‑149 (MQ=255)
                                                 gcagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCt                                                                                                    <  1:58779/149‑1 (MQ=255)
                                                 gcagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCt                                                                                                    <  2:204034/149‑1 (MQ=255)
                                                  cagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCtt                                                                                                   <  1:64178/149‑1 (MQ=255)
                                                   agcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTACAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTc                                                                                                  <  2:1176092/149‑1 (MQ=255)
                                                    aaccGCGACGCTCATCATCAAGCTCAGAGGAAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTcc                                                                                                 <  2:1335236/147‑1 (MQ=255)
                                                     cccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCa                                                                                                <  2:436615/149‑1 (MQ=255)
                                                     cccGCGACGCTCATAATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGCACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGACCAAGCGCTAAGGTATCTTccc                                                                                                >  2:962471/1‑148 (MQ=255)
                                                      ccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCAc                                                                                               <  1:656339/149‑1 (MQ=255)
                                                      ccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCGTAAGCTGCCCACGCGATAAGGTATCTTCCAc                                                                                               >  1:782097/1‑149 (MQ=255)
                                                              cTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAc                                                                                       >  2:80353/1‑149 (MQ=255)
                                                               tcatcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACTGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGAGCTCATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAcc                                                                                      >  1:516546/1‑149 (MQ=255)
                                                               tcatcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGACCTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAcc                                                                                      >  2:486683/1‑149 (MQ=255)
                                                               tcatcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAcc                                                                                      >  1:1144411/1‑149 (MQ=255)
                                                               tcatcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAcc                                                                                      >  1:940522/1‑149 (MQ=255)
                                                                  tcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTc                                                                                   <  2:1563644/149‑1 (MQ=255)
                                                                   catcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCg                                                                                  >  1:485630/1‑149 (MQ=255)
                                                                    atcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCCCATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCgg                                                                                 >  1:304937/1‑149 (MQ=255)
                                                                      caAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGgcg                                                                               >  2:911825/1‑149 (MQ=255)
                                                                             agagGCAAAATCATCTATCAGGTAGAGAAACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGc                                                                        <  1:1209238/149‑1 (MQ=255)
                                                                               agGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGATGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGt                                                                      >  1:199944/1‑149 (MQ=255)
                                                                                 gCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTaa                                                                    >  2:1433335/1‑149 (MQ=255)
                                                                                   aaaaTCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGt                                                                  <  2:492082/149‑1 (MQ=255)
                                                                                        catcTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCt                                                             <  2:637787/149‑1 (MQ=255)
                                                                                          tcTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCttt                                                           >  2:768112/1‑149 (MQ=255)
                                                                                            tATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgt                                                         <  1:1058037/149‑1 (MQ=255)
                                                                                             aTCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgtg                                                        >  1:1087318/1‑149 (MQ=255)
                                                                                                aGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCgg                                                     >  2:1126080/1‑149 (MQ=255)
                                                                                                 ggTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAATCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg                                                    >  1:1516882/1‑149 (MQ=255)
                                                                                                 ggTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg                                                    >  2:894315/1‑149 (MQ=255)
                                                                                                  gTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATATGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGc                                                   >  2:816280/1‑149 (MQ=255)
                                                                                                  gTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGc                                                   <  1:365458/149‑1 (MQ=255)
                                                                                                   tAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGcc                                                  >  2:1288460/1‑149 (MQ=255)
                                                                                                      agaCACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTg                                               >  1:208820/1‑149 (MQ=255)
                                                                                                       gaCACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTgc                                              >  1:1224820/1‑149 (MQ=255)
                                                                                                         cacCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGAGCAACGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTgcgc                                            >  2:1093589/1‑149 (MQ=255)
                                                                                                             gccgccCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGgtg                                        <  2:1561365/149‑1 (MQ=255)
                                                                                                                gcccgcTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAg                                     <  2:355160/149‑1 (MQ=255)
                                                                                                                   cgcTTTCACGGGTGAGGAACTCTCCTTGCGCCAGATGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGtt                                  <  2:199944/149‑1 (MQ=255)
                                                                                                                    gcTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTa                                 <  2:1224820/149‑1 (MQ=255)
                                                                                                                            gggTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGa                         <  2:88576/149‑1 (MQ=255)
                                                                                                                              gTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATc                       >  1:1543778/1‑149 (MQ=255)
                                                                                                                                gAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACCGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATcgc                     <  1:962471/149‑1 (MQ=255)
                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAATCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                   <  2:1516882/149‑1 (MQ=255)
                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                   <  2:954163/149‑1 (MQ=255)
                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                   <  2:208820/149‑1 (MQ=255)
                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                   <  2:1523363/149‑1 (MQ=255)
                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                   >  1:947627/1‑149 (MQ=255)
                                                                                                                                   gAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGtt                  <  2:264306/149‑1 (MQ=255)
                                                                                                                                    aaCTCTCCTTGCGCCAGACGTAAGGCTCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTc                 >  2:1496511/1‑149 (MQ=255)
                                                                                                                                    aaCTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTc                 <  2:1543778/149‑1 (MQ=255)
                                                                                                                                      gttgatgtGCGCCTTACGTCTGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaa               >  1:1385050/8‑149 (MQ=255)
                                                                                                                                      gttgatgtGCGCCTTACGTCTGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaa               >  1:238901/8‑149 (MQ=255)
                                                                                                                                       ttgatgtGCGCCTTACGTCTGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaa              >  1:1017358/7‑149 (MQ=255)
                                                                                                                                       tctcCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaa              >  2:1003020/1‑149 (MQ=255)
                                                                                                                                        ctcCTTGCGCCAGTCGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaaa             <  1:456658/149‑1 (MQ=255)
                                                                                                                                        ctcCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaaa             >  1:1561381/1‑149 (MQ=255)
                                                                                                                                         tcCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAAt            >  1:1192850/1‑149 (MQ=255)
                                                                                                                                          ccTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGTGCACCGTCGGCGCGAATTCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAAtt           <  1:1093589/149‑1 (MQ=255)
                                                                                                                                           tgtGCGCCTTACGTCTGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTa          >  2:338195/3‑149 (MQ=255)
                                                                                                                                           cTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTa          >  2:1529021/1‑149 (MQ=255)
                                                                                                                                           cTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTa          <  2:1188889/149‑1 (MQ=255)
                                                                                                                                            ttGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTAc         <  1:1126080/149‑1 (MQ=255)
                                                                                                                                             tGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACg        <  1:221205/149‑1 (MQ=255)
                                                                                                                                                 ccAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTcc    >  1:736771/1‑149 (MQ=255)
                                                                                                                                                   aGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAg  <  2:454785/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAG  >  NC_000913/3880227‑3880522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑