BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,880,375	G→T	6.1%	L283M (CTG→ATG) ‡	recF ←	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,880,375	0	G	T	6.1%	316.1 / 8.5	98	L283M (CTG→ATG) ‡	recF	gap repair protein
Reads supporting (aligned to +/- strand): ref base G (48/44); new base T (4/2); total (52/46)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

Only 100 of 101 total aligned reads displayed.

ATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGC  >  NC_000913/3880227‑3880523

aTCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAg                                                                                                                                                      >  1:105903/1‑149 (MQ=255)

aTCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAg                                                                                                                                                      >  1:101439/1‑149 (MQ=255)

 tCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGa                                                                                                                                                     >  2:656339/1‑149 (MQ=255)

 tCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGa                                                                                                                                                     <  2:508287/149‑1 (MQ=255)

   gcgcTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCAACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACg                                                                                                                                                   >  2:955893/1‑149 (MQ=255)

           aaaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGCCGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGCCACCGCCGCCCGCTGTCACGGGTGAGGATCTCTCCTTGCGCCAGACGTAAGgcgc                                                                                                                                           >  2:425304/1‑149 (MQ=255)

           aaaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcgc                                                                                                                                           >  2:221205/1‑149 (MQ=255)

           aaaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcgc                                                                                                                                           >  1:1572422/1‑149 (MQ=255)

              gACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCTTACGTCTGGCGcaag                                                                                                                                        <  1:1167531/149‑3 (MQ=255)

                ccTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATc                                                                                                                                      <  2:105903/149‑1 (MQ=255)

                ccTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATc                                                                                                                                      <  2:101439/149‑1 (MQ=255)

                ccTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCGTGCGCCTTACGTCTGGCGcaagga                                                                                                                                      <  1:98604/149‑5 (MQ=255)

                  tgtgATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCaa                                                                                                                                    >  2:1139516/1‑149 (MQ=255)

                    tgATTGCGTCGCTTTTAAGCGGCTAGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACa                                                                                                                                  <  2:236014/149‑1 (MQ=255)

                       ttGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCt                                                                                                                               <  1:695658/149‑1 (MQ=255)

                       ttGCGGCGCGTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCt                                                                                                                               <  1:908128/149‑1 (MQ=255)

                           gTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGCCACCGCCGCCCGCTTTCACGGGTGAGGCACTCTCCGTGCGCCAGACGTACGGCGCACCTCAGCAGCTTAAg                                                                                                                           >  1:278837/1‑149 (MQ=255)

                           gTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAg                                                                                                                           <  2:877758/149‑1 (MQ=255)

                           gTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAg                                                                                                                           >  1:698268/1‑149 (MQ=255)

                           gTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAg                                                                                                                           >  1:264306/1‑149 (MQ=255)

                            tCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGc                                                                                                                          <  2:47560/149‑1 (MQ=255)

                            tCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGc                                                                                                                          >  1:1523363/1‑149 (MQ=255)

                               cTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                                                                       <  1:1171833/149‑1 (MQ=255)

                               cTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                                                                       <  1:862534/149‑1 (MQ=255)

                                ttttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCTAAATCATCTATCAGGAAGAGCCACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACCGCGTAAGCTGcc                                                                                                                      >  2:316702/1‑149 (MQ=255)

                                          cTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATaa                                                                                                            >  1:355160/1‑149 (MQ=255)

                                           tGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAg                                                                                                           >  1:27512/1‑149 (MQ=255)

                                           tGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAg                                                                                                           >  1:454785/1‑149 (MQ=255)

                                            ggCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAgg                                                                                                          <  1:567386/149‑1 (MQ=255)

                                             gCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAg                                                                                                                                 >  2:512052/1‑125 (MQ=255)

                                             gCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAg                                                                                                                                 <  1:512052/125‑1 (MQ=255)

                                             gCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGt                                                                                                         <  1:793333/149‑1 (MQ=255)

                                              cAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTa                                                                                                        >  2:194838/1‑149 (MQ=255)

                                              cAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTa                                                                                                        >  2:440101/1‑149 (MQ=255)

                                               aaGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTAt                                                                                                       >  1:630465/1‑149 (MQ=255)

                                                 gcagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCt                                                                                                     <  1:58779/149‑1 (MQ=255)

                                                 gcagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCt                                                                                                     <  2:204034/149‑1 (MQ=255)

                                                  cagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCtt                                                                                                    <  1:64178/149‑1 (MQ=255)

                                                   agcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTACAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTc                                                                                                   <  2:1176092/149‑1 (MQ=255)

                                                    aaccGCGACGCTCATCATCAAGCTCAGAGGAAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTcc                                                                                                  <  2:1335236/147‑1 (MQ=255)

                                                     cccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCa                                                                                                 <  2:436615/149‑1 (MQ=255)

                                                      ccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCAc                                                                                                <  1:656339/149‑1 (MQ=255)

                                                      ccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCGTAAGCTGCCCACGCGATAAGGTATCTTCCAc                                                                                                >  1:782097/1‑149 (MQ=255)

                                                              cTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAc                                                                                        >  2:80353/1‑149 (MQ=255)

                                                               tcatcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACTGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGAGCTCATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAcc                                                                                       >  1:516546/1‑149 (MQ=255)

                                                               tcatcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGACCTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAcc                                                                                       >  2:486683/1‑149 (MQ=255)

                                                               tcatcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAcc                                                                                       >  1:940522/1‑149 (MQ=255)

                                                               tcatcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCAcc                                                                                       >  1:1144411/1‑149 (MQ=255)

                                                                  tcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTc                                                                                    <  2:1563644/149‑1 (MQ=255)

                                                                   catcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCg                                                                                   >  1:485630/1‑149 (MQ=255)

                                                                    atcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCCCATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCgg                                                                                  >  1:304937/1‑149 (MQ=255)

                                                                      caAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGgcg                                                                                >  2:911825/1‑149 (MQ=255)

                                                                             agagGCAAAATCATCTATCAGGTAGAGAAACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGc                                                                         <  1:1209238/149‑1 (MQ=255)

                                                                               agGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGATGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGt                                                                       >  1:199944/1‑149 (MQ=255)

                                                                                 gCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTaa                                                                     >  2:1433335/1‑149 (MQ=255)

                                                                                   aaaaTCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGt                                                                   <  2:492082/149‑1 (MQ=255)

                                                                                        catcTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCt                                                              <  2:637787/149‑1 (MQ=255)

                                                                                          tcTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCttt                                                            >  2:768112/1‑149 (MQ=255)

                                                                                            tATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgt                                                          <  1:1058037/149‑1 (MQ=255)

                                                                                             aTCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgtg                                                         >  1:1087318/1‑149 (MQ=255)

                                                                                                aGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCgg                                                      >  2:1126080/1‑149 (MQ=255)

                                                                                                 ggTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAATCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg                                                     >  1:1516882/1‑149 (MQ=255)

                                                                                                 ggTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg                                                     >  2:894315/1‑149 (MQ=255)

                                                                                                  gTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATATGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGc                                                    >  2:816280/1‑149 (MQ=255)

                                                                                                  gTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGc                                                    <  1:365458/149‑1 (MQ=255)

                                                                                                   tAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGcc                                                   >  2:1288460/1‑149 (MQ=255)

                                                                                                      agaCACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTg                                                >  1:208820/1‑149 (MQ=255)

                                                                                                       gaCACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTgc                                               >  1:1224820/1‑149 (MQ=255)

                                                                                                         cacCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGAGCAACGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTgcgc                                             >  2:1093589/1‑149 (MQ=255)

                                                                                                             gccgccCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGgtg                                         <  2:1561365/149‑1 (MQ=255)

                                                                                                                gcccgcTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAg                                      <  2:355160/149‑1 (MQ=255)

                                                                                                                   cgcTTTCACGGGTGAGGAACTCTCCTTGCGCCAGATGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGtt                                   <  2:199944/149‑1 (MQ=255)

                                                                                                                    gcTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTa                                  <  2:1224820/149‑1 (MQ=255)

                                                                                                                            gggTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGa                          <  2:88576/149‑1 (MQ=255)

                                                                                                                              gTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATc                        >  1:1543778/1‑149 (MQ=255)

                                                                                                                                gAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACCGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATcgc                      <  1:962471/149‑1 (MQ=255)

                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAATCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                    <  2:1516882/149‑1 (MQ=255)

                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                    <  2:1523363/149‑1 (MQ=255)

                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                    <  2:208820/149‑1 (MQ=255)

                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                    >  1:947627/1‑149 (MQ=255)

                                                                                                                                  ggAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGt                    <  2:954163/149‑1 (MQ=255)

                                                                                                                                   gAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGtt                   <  2:264306/149‑1 (MQ=255)

                                                                                                                                    aaCTCTCCTTGCGCCAGACGTAAGGCTCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTc                  >  2:1496511/1‑149 (MQ=255)

                                                                                                                                    aaCTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTc                  <  2:1543778/149‑1 (MQ=255)

                                                                                                                                      gttgatgtGCGCCTTACGTCTGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaa                >  1:1385050/8‑149 (MQ=255)

                                                                                                                                      gttgatgtGCGCCTTACGTCTGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaa                >  1:238901/8‑149 (MQ=255)

                                                                                                                                       ttgatgtGCGCCTTACGTCTGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaa               >  1:1017358/7‑149 (MQ=255)

                                                                                                                                       tctcCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaa               >  2:1003020/1‑149 (MQ=255)

                                                                                                                                        ctcCTTGCGCCAGTCGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaaa              <  1:456658/149‑1 (MQ=255)

                                                                                                                                        ctcCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCaaaa              >  1:1561381/1‑149 (MQ=255)

                                                                                                                                         tcCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAAt             >  1:1192850/1‑149 (MQ=255)

                                                                                                                                          ccTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGTGCACCGTCGGCGCGAATTCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAAtt            <  1:1093589/149‑1 (MQ=255)

                                                                                                                                           tgtGCGCCTTACGTCTGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTa           >  2:338195/3‑149 (MQ=255)

                                                                                                                                           cTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTa           >  2:1529021/1‑149 (MQ=255)

                                                                                                                                           cTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTa           <  2:1188889/149‑1 (MQ=255)

                                                                                                                                            ttGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTAc          <  1:1126080/149‑1 (MQ=255)

                                                                                                                                             tGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACg         <  1:221205/149‑1 (MQ=255)

                                                                                                                                                 ccAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTcc     >  1:736771/1‑149 (MQ=255)

                                                                                                                                                   aGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAg   <  2:454785/149‑1 (MQ=255)

                                                                                                                                                    gACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGc  <  1:80353/149‑1 (MQ=255)

ATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCGTGCGCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGC  >  NC_000913/3880227‑3880523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`