Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,953,432 C→T 6.2% intergenic (+19/‑46) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,953,4320CT 6.2% 250.9 / 4.5 80intergenic (+19/‑46)ilvE/ilvDbranched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (30/45);  new base T (3/2);  total (33/47)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-01

CGTAGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTT  >  NC_000913/3953284‑3953572
                                                                                                                                                    |                                                                                                                                            
cGTAGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgc                                                                                                                                              <  2:1327981/149‑1 (MQ=255)
    gACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgc                                                                                                                                          >  2:725831/1‑149 (MQ=255)
           ttCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCACACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGCCTGGGTAGATCAAGTTAATCAATAAATACAAAAAATGTGACGGCACGCACCGTcc                                                                                                                                   >  1:1333262/1‑149 (MQ=255)
           ttCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGTTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTcc                                                                                                                                   >  2:1324320/1‑149 (MQ=255)
           ttCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTcc                                                                                                                                   <  1:1022029/149‑1 (MQ=255)
            tCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGTCCTCTTCACTGGCGAAAGCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTccc                                                                                                                                  <  2:112565/149‑1 (MQ=255)
            tCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTccc                                                                                                                                  <  2:988568/149‑1 (MQ=255)
            tCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTccc                                                                                                                                  <  1:1237194/149‑1 (MQ=255)
             cAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCa                                                                                                                                 >  2:1346844/1‑149 (MQ=255)
                      gAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACgaga                                                                                                                        <  1:708714/149‑1 (MQ=255)
                      gAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACgaga                                                                                                                        <  1:1221505/149‑1 (MQ=255)
                                gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                              <  1:440464/149‑1 (MQ=255)
                                gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                              <  1:567193/149‑1 (MQ=255)
                                gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                              <  2:227414/149‑1 (MQ=255)
                                gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                              <  2:71699/149‑1 (MQ=255)
                                  ggCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTACTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGa                                                                                                            <  2:316737/149‑1 (MQ=255)
                                     ccGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGta                                                                                                         <  1:1421091/149‑1 (MQ=255)
                                      cGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaa                                                                                                        <  2:1227496/149‑1 (MQ=255)
                                          tACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataa                                                                                                    >  2:1312695/1‑149 (MQ=255)
                                              aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTa                                                                                                >  2:1506891/1‑149 (MQ=255)
                                               aaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATTGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                               >  1:1154539/1‑149 (MQ=255)
                                               aaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                               <  1:912544/149‑1 (MQ=255)
                                               aaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                               <  1:66773/149‑1 (MQ=255)
                                                 cGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGc                                                                                             <  2:908724/149‑1 (MQ=255)
                                                  gCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGcc                                                                                            <  2:182858/149‑1 (MQ=255)
                                                  gCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGcc                                                                                            <  2:963965/149‑1 (MQ=255)
                                                    aTTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTa                                                                                          >  1:1386382/1‑149 (MQ=255)
                                                         gCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAc                                                                                     >  2:1128640/1‑149 (MQ=255)
                                                         gCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAc                                                                                     <  1:1098609/149‑1 (MQ=255)
                                                          cAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAcc                                                                                    >  2:989006/1‑149 (MQ=255)
                                                            aGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCCCGCACCGTCCCATTTACGAGACAGACACTGGGAGTATATAAAGAATGCCTAAGTACCGt                                                                                  >  1:1079458/1‑149 (MQ=255)
                                                               cttcttCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTcc                                                                               >  2:1022447/1‑149 (MQ=255)
                                                                 tcttcGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGc                                                                             <  2:918329/149‑1 (MQ=255)
                                                                      ggCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccacca                                                                        <  2:1386382/149‑1 (MQ=255)
                                                                      ggCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccacca                                                                        <  1:1525415/149‑1 (MQ=255)
                                                                        ccTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACTGACACTGGGAGTAAATAAAGTATGCCTAAGTACAGTTCCGacaccacc                                                                      >  2:612170/1‑149 (MQ=255)
                                                                        ccTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccaccacc                                                                      <  1:1458428/149‑1 (MQ=255)
                                                                           ctTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAACTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACt                                                                   <  2:190668/149‑1 (MQ=255)
                                                                            ttCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTc                                                                  >  2:725069/1‑149 (MQ=255)
                                                                              cACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCAt                                                                >  2:985609/1‑149 (MQ=255)
                                                                               aCTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATg                                                               <  1:1022447/149‑1 (MQ=255)
                                                                               aCTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAAAAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATg                                                               <  1:149648/149‑1 (MQ=255)
                                                                                 tGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGTCAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGt                                                             <  2:1087882/149‑1 (MQ=255)
                                                                                 tGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGt                                                             <  1:418962/149‑1 (MQ=255)
                                                                                    cGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGt                                                          <  1:1180237/149‑1 (MQ=255)
                                                                                    cGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGt                                                          <  2:123642/149‑1 (MQ=255)
                                                                                      aaaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTaa                                                        <  1:159951/149‑1 (MQ=255)
                                                                                      aaaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTaa                                                        <  1:619995/149‑1 (MQ=255)
                                                                                      aaaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGCGACGGCACGCACCGTCCCATTTACGAGAAATACACAGGGAGTAAAAAAAGTATGCCAAAGAACCGTTCCGACACCACCACTCATGGACGTaa                                                        >  1:1588446/1‑149 (MQ=255)
                                                                                         ccGAAGATAACTGGGGCTGGTTAGATCATGTTCATCTATCACTACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAatat                                                     <  2:234887/149‑1 (MQ=255)
                                                                                          cGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATg                                                    >  1:115199/1‑149 (MQ=255)
                                                                                            aaGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGc                                                  <  2:216699/149‑1 (MQ=255)
                                                                                              gATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCgg                                                >  1:1390833/1‑149 (MQ=255)
                                                                                                 aaaTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTg                                             <  2:1326393/149‑1 (MQ=255)
                                                                                                    tGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAATTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTc                                          <  1:1364973/149‑1 (MQ=255)
                                                                                                       ggCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAGGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTg                                       >  2:1179489/1‑149 (MQ=255)
                                                                                                            gTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtg                                  >  2:1366501/1‑149 (MQ=255)
                                                                                                            gTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtg                                  >  2:783992/1‑149 (MQ=255)
                                                                                                                gATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgc                              >  2:929370/1‑149 (MQ=255)
                                                                                                                  tCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgc                            <  1:612170/149‑1 (MQ=255)
                                                                                                                   cAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCCCCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgcg                           >  1:682670/1‑149 (MQ=255)
                                                                                                                      gatAATCAATAAATACAAAAAAAGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCa                        <  1:235259/147‑1 (MQ=255)
                                                                                                                      gTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTACGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGccc                        >  2:1510481/1‑148 (MQ=255)
                                                                                                                       ttAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCAc                       >  2:1246431/1‑149 (MQ=255)
                                                                                                                         aaTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCg                     >  1:1073752/1‑149 (MQ=255)
                                                                                                                           tCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCTCCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGa                   <  2:446474/149‑1 (MQ=255)
                                                                                                                           tCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCCCCCCTCATGGTCGTAATTTGGCGGGTGCTCGTGCGCTGTGGCTCGCCACCGGa                   >  2:1001816/1‑149 (MQ=255)
                                                                                                                            cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCTCCGGaa                  <  1:592166/149‑1 (MQ=255)
                                                                                                                            cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCTCCGGaa                  <  1:597826/149‑1 (MQ=255)
                                                                                                                             aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTTATATGGCGGGTGCTCGTGCGCTNTGGCGCGCCACCngnnn                 <  2:27839/149‑4 (MQ=255)
                                                                                                                             aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAAt                 <  1:257288/149‑1 (MQ=255)
                                                                                                                             aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGACACCAACACTCATTGTCGAAATATGGCGGGTGCTCGTGCTCTGTGGCGCGCCACCGGAAt                 >  2:997460/1‑149 (MQ=255)
                                                                                                                                 aataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGCCACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGAcc             >  1:1162239/1‑149 (MQ=255)
                                                                                                                                     tcgtaaaTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACg         >  2:996563/5‑149 (MQ=255)
                                                                                                                                       aaaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGcc       >  2:306431/1‑149 (MQ=255)
                                                                                                                                       aaaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGcc       >  2:1146448/1‑149 (MQ=255)
                                                                                                                                        taaaTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCg      >  2:712087/2‑149 (MQ=255)
                                                                                                                                         aaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCATAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGa     <  2:1154539/149‑1 (MQ=255)
                                                                                                                                          aaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAt    <  1:1144938/149‑1 (MQ=255)
                                                                                                                                          aaTGGGACCGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAt    <  1:412772/149‑1 (MQ=255)
                                                                                                                                           aTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGCCGCCGAtt   >  2:160975/1‑149 (MQ=255)
                                                                                                                                            tGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAttt  <  1:101144/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CGTAGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTT  >  NC_000913/3953284‑3953572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑