Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,953,433 A→G 6.3% intergenic (+20/‑45) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,953,4330AG 6.3% 237.2 / 5.1 80intergenic (+20/‑45)ilvE/ilvDbranched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base A (29/45);  new base G (3/2);  total (33/47)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

GACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCC  >  NC_000913/3953288‑3953581
                                                                                                                                                 |                                                                                                                                                    
gACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgc                                                                                                                                                   >  2:725831/1‑149 (MQ=255)
       ttCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCACACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGCCTGGGTAGATCAAGTTAATCAATAAATACAAAAAATGTGACGGCACGCACCGTcc                                                                                                                                            >  1:1333262/1‑149 (MQ=255)
       ttCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGTTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTcc                                                                                                                                            >  2:1324320/1‑149 (MQ=255)
       ttCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTcc                                                                                                                                            <  1:1022029/149‑1 (MQ=255)
        tCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGTCCTCTTCACTGGCGAAAGCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTccc                                                                                                                                           <  2:112565/149‑1 (MQ=255)
        tCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTccc                                                                                                                                           <  2:988568/149‑1 (MQ=255)
        tCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTccc                                                                                                                                           <  1:1237194/149‑1 (MQ=255)
         cAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCa                                                                                                                                          >  2:1346844/1‑149 (MQ=255)
                  gAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACgaga                                                                                                                                 <  1:708714/149‑1 (MQ=255)
                  gAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACgaga                                                                                                                                 <  1:1221505/149‑1 (MQ=255)
                            gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                       <  2:71699/149‑1 (MQ=255)
                            gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                       <  2:227414/149‑1 (MQ=255)
                            gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                       <  1:440464/149‑1 (MQ=255)
                            gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                       <  1:567193/149‑1 (MQ=255)
                              ggCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTACTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGa                                                                                                                     <  2:316737/149‑1 (MQ=255)
                                 ccGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGta                                                                                                                  <  1:1421091/149‑1 (MQ=255)
                                  cGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaa                                                                                                                 <  2:1227496/149‑1 (MQ=255)
                                      tACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataa                                                                                                             >  2:1312695/1‑149 (MQ=255)
                                          aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTa                                                                                                         >  2:1506891/1‑149 (MQ=255)
                                           aaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATTGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                                        >  1:1154539/1‑149 (MQ=255)
                                           aaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                                        <  1:66773/149‑1 (MQ=255)
                                           aaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                                        <  1:912544/149‑1 (MQ=255)
                                             cGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGc                                                                                                      <  2:908724/149‑1 (MQ=255)
                                              gCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGcc                                                                                                     <  2:963965/149‑1 (MQ=255)
                                              gCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGcc                                                                                                     <  2:182858/149‑1 (MQ=255)
                                                aTTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTa                                                                                                   >  1:1386382/1‑149 (MQ=255)
                                                     gCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAc                                                                                              >  2:1128640/1‑149 (MQ=255)
                                                     gCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAc                                                                                              <  1:1098609/149‑1 (MQ=255)
                                                      cAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAcc                                                                                             >  2:989006/1‑149 (MQ=255)
                                                        aGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCCCGCACCGTCCCATTTACGAGACAGACACTGGGAGTATATAAAGAATGCCTAAGTACCGt                                                                                           >  1:1079458/1‑149 (MQ=255)
                                                           cttcttCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTcc                                                                                        >  2:1022447/1‑149 (MQ=255)
                                                             tcttcGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGc                                                                                      <  2:918329/149‑1 (MQ=255)
                                                                  ggCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccacca                                                                                 <  2:1386382/149‑1 (MQ=255)
                                                                  ggCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccacca                                                                                 <  1:1525415/149‑1 (MQ=255)
                                                                    ccTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACTGACACTGGGAGTAAATAAAGTATGCCTAAGTACAGTTCCGacaccacc                                                                               >  2:612170/1‑149 (MQ=255)
                                                                    ccTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccaccacc                                                                               <  1:1458428/149‑1 (MQ=255)
                                                                       ctTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAACTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACt                                                                            <  2:190668/149‑1 (MQ=255)
                                                                        ttCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTc                                                                           >  2:725069/1‑149 (MQ=255)
                                                                          cACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCAt                                                                         >  2:985609/1‑149 (MQ=255)
                                                                           aCTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATg                                                                        <  1:1022447/149‑1 (MQ=255)
                                                                           aCTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAAAAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATg                                                                        <  1:149648/149‑1 (MQ=255)
                                                                             tGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGTCAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGt                                                                      <  2:1087882/149‑1 (MQ=255)
                                                                             tGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGt                                                                      <  1:418962/149‑1 (MQ=255)
                                                                                cGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGt                                                                   <  2:123642/149‑1 (MQ=255)
                                                                                cGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGt                                                                   <  1:1180237/149‑1 (MQ=255)
                                                                                  aaaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTaa                                                                 <  1:159951/149‑1 (MQ=255)
                                                                                  aaaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTaa                                                                 <  1:619995/149‑1 (MQ=255)
                                                                                  aaaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGCGACGGCACGCACCGTCCCATTTACGAGAAATACACAGGGAGTAAAAAAAGTATGCCAAAGAACCGTTCCGACACCACCACTCATGGACGTaa                                                                 >  1:1588446/1‑149 (MQ=255)
                                                                                     ccGAAGATAACTGGGGCTGGTTAGATCATGTTCATCTATCACTACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAatat                                                              <  2:234887/149‑1 (MQ=255)
                                                                                      cGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATg                                                             >  1:115199/1‑149 (MQ=255)
                                                                                        aaGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGc                                                           <  2:216699/149‑1 (MQ=255)
                                                                                          gATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCgg                                                         >  1:1390833/1‑149 (MQ=255)
                                                                                             aaaTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTg                                                      <  2:1326393/149‑1 (MQ=255)
                                                                                                tGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAATTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTc                                                   <  1:1364973/149‑1 (MQ=255)
                                                                                                   ggCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAGGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTg                                                >  2:1179489/1‑149 (MQ=255)
                                                                                                        gTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtg                                           >  2:1366501/1‑149 (MQ=255)
                                                                                                        gTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtg                                           >  2:783992/1‑149 (MQ=255)
                                                                                                            gATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgc                                       >  2:929370/1‑149 (MQ=255)
                                                                                                              tCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgc                                     <  1:612170/149‑1 (MQ=255)
                                                                                                               cAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCCCCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgcg                                    >  1:682670/1‑149 (MQ=255)
                                                                                                                  gatAATCAATAAATACAAAAAAAGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCa                                 <  1:235259/147‑1 (MQ=255)
                                                                                                                  gTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTACGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGccc                                 >  2:1510481/1‑148 (MQ=255)
                                                                                                                   ttAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCAc                                >  2:1246431/1‑149 (MQ=255)
                                                                                                                     aaTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCg                              >  1:1073752/1‑149 (MQ=255)
                                                                                                                       tCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCTCCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGa                            <  2:446474/149‑1 (MQ=255)
                                                                                                                       tCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCCCCCCTCATGGTCGTAATTTGGCGGGTGCTCGTGCGCTGTGGCTCGCCACCGGa                            >  2:1001816/1‑149 (MQ=255)
                                                                                                                        cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCTCCGGaa                           <  1:597826/149‑1 (MQ=255)
                                                                                                                        cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCTCCGGaa                           <  1:592166/149‑1 (MQ=255)
                                                                                                                         aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTTATATGGCGGGTGCTCGTGCGCTNTGGCGCGCCACCngnnn                          <  2:27839/149‑4 (MQ=255)
                                                                                                                         aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAAt                          <  1:257288/149‑1 (MQ=255)
                                                                                                                         aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGACACCAACACTCATTGTCGAAATATGGCGGGTGCTCGTGCTCTGTGGCGCGCCACCGGAAt                          >  2:997460/1‑149 (MQ=255)
                                                                                                                             aataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGCCACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGAcc                      >  1:1162239/1‑149 (MQ=255)
                                                                                                                                 tcgtaaaTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACg                  >  2:996563/5‑149 (MQ=255)
                                                                                                                                   aaaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGcc                >  2:306431/1‑149 (MQ=255)
                                                                                                                                   aaaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGcc                >  2:1146448/1‑149 (MQ=255)
                                                                                                                                    taaaTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCg               >  2:712087/2‑149 (MQ=255)
                                                                                                                                     aaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCATAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGa              <  2:1154539/149‑1 (MQ=255)
                                                                                                                                      aaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAt             <  1:1144938/149‑1 (MQ=255)
                                                                                                                                      aaTGGGACCGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAt             <  1:412772/149‑1 (MQ=255)
                                                                                                                                       aTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGCCGCCGAtt            >  2:160975/1‑149 (MQ=255)
                                                                                                                                        tGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAttt           <  1:101144/149‑1 (MQ=255)
                                                                                                                                                 acgcacCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGcc  >  2:1179873/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCC  >  NC_000913/3953288‑3953581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑