Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,967,553 A→T 7.1% K379N (AAA→AAT rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,967,5530AT 7.1% 338.1 / 11.4 113K379N (AAA→AATrhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base A (65/40);  new base T (3/5);  total (68/45)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 113 total aligned reads displayed.

CTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGC  >  NC_000913/3967409‑3967698
                                                                                                                                                |                                                                                                                                                 
cTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGCTCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCAGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgt                                                                                                                                               >  2:1152671/1‑149 (MQ=255)
cTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgt                                                                                                                                               >  1:610713/1‑149 (MQ=255)
 tACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAATGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgtg                                                                                                                                              >  2:100749/1‑149 (MQ=255)
 tACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgtg                                                                                                                                              >  2:1365742/1‑149 (MQ=255)
 tACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCACGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgtg                                                                                                                                              >  2:274484/1‑149 (MQ=255)
   cGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGa                                                                                                                                            >  1:1514027/1‑149 (MQ=255)
    gAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt                                                                                                                                           >  2:479688/1‑149 (MQ=255)
     aaGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGTGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATc                                                                                                                                          >  1:1479245/1‑149 (MQ=255)
     aaGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAATATGTGGATc                                                                                                                                          >  1:1472182/1‑149 (MQ=255)
         gTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTgc                                                                                                                                      <  1:1106874/149‑1 (MQ=255)
            tAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTACCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCa                                                                                                                                   >  1:1401000/1‑149 (MQ=255)
             aaaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCACGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGACCTGCAGAAAATGTGGATCCTGCGCaa                                                                                                                                  >  1:92028/1‑149 (MQ=255)
              aaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCaaa                                                                                                                                 >  2:1499109/1‑149 (MQ=255)
                 gTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATc                                                                                                                              >  2:633105/1‑149 (MQ=255)
                  tACAGGAAAAATGGAACTGAACCTCTCTCGTAAGATCGCTGAAAAACGGGTCTTCCCGGATATTTACTACAACTGTTGTGTTACCCGTAAATAATAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCa                                                                                                                             <  1:698961/149‑1 (MQ=255)
                   aCAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACACCCGTTCTGGTCCCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCAt                                                                                                                            <  2:1475903/149‑1 (MQ=255)
                    cAGGCACCATGGAACTGCACCTCTCTCGTATGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTAGAAGCGTTGTGGTACCCGTAAAGATGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCAtt                                                                                                                           <  1:470790/149‑1 (MQ=255)
                      ggCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTcc                                                                                                                         >  2:682967/1‑148 (MQ=255)
                       gCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCAc                                                                                                                        >  2:1079242/1‑149 (MQ=255)
                        cAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCAcc                                                                                                                       >  2:924043/1‑149 (MQ=255)
                         aaCATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCAccc                                                                                                                      >  2:1539722/1‑149 (MQ=255)
                         aaCAGGGACCTGCACCTCTCTCCTAAGATCGCGGAAAAACGCGTCTTCCCGGCTATCGCCTCCAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAATATGTGGATCCTGCGCAAAATCATTCAccc                                                                                                                      <  2:395797/149‑1 (MQ=255)
                             tGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATg                                                                                                                  >  2:1206981/1‑149 (MQ=255)
                              ggAACTGCACCTCTCTCGTAAGATGGCTGAAAAAGGCGTCTTCCCGGCTATCGACTACACCCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATgg                                                                                                                 <  2:92028/149‑1 (MQ=255)
                               gAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAATATGTGGATCCTGCGCAAAATCATTCACCCGATggg                                                                                                                >  1:345717/1‑149 (MQ=255)
                                 aCTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACACCCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCg                                                                                                              >  2:85327/1‑149 (MQ=255)
                                    gCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGaaa                                                                                                           >  2:847123/1‑149 (MQ=255)
                                     cACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAAt                                                                                                          >  2:552561/1‑149 (MQ=255)
                                       ccTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCg                                                                                                        >  2:1522841/1‑149 (MQ=255)
                                        ctctctCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGa                                                                                                       >  2:173692/1‑149 (MQ=255)
                                        cgctctctTCAGAGAGCTCAAAAACGCGTCGTCCAGGCTATCGACTACAACCGTTCTGTTACCCGTAAAGAAGAGCTGGTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGa                                                                                                       <  2:259124/147‑1 (MQ=255)
                                         tctctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCCGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGAt                                                                                                      <  1:937777/149‑1 (MQ=255)
                                             tcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCaa                                                                                                  <  1:1116458/149‑1 (MQ=255)
                                               gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATg                                                                                                >  2:134941/1‑149 (MQ=255)
                                                tAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATTCAATgg                                                                                               >  2:1447518/1‑149 (MQ=255)
                                                    aTCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAAtt                                                                                           >  2:448582/1‑149 (MQ=255)
                                                      cGCTGAAAAACGCGTATTCCCGGATATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACTACTCAGGAAGAACTGCAGAAAATGTGGATACTGCGCAAAATCATTCACCCGATGGGCGAATTCGATGCAATGGAATTcc                                                                                         <  2:70635/149‑1 (MQ=255)
                                                       gCTGAAAAACGCGTCTTCCCGGCTATCGACTAAAACCGTTCTGTTACCCGAAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCt                                                                                        <  1:1535583/149‑1 (MQ=255)
                                                        ctcaaacaCGCGTCTTACCCGCTCTCGACTACAACCGTGCAGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGACGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCCCCCGATGGGCGAAATCGATGCAATGGAATTCCTc                                                                                       <  2:1486317/146‑1 (MQ=255)
                                                        cTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTc                                                                                       >  2:820406/1‑149 (MQ=255)
                                                         tGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCa                                                                                      >  1:546744/1‑149 (MQ=255)
                                                          gAAAAACGCGTCTTCCCGGCTTTCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCAt                                                                                     <  1:1581747/149‑1 (MQ=255)
                                                          gAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCAt                                                                                     >  2:826271/1‑149 (MQ=255)
                                                               ccgcgTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATtaata                                                                                <  1:552561/148‑1 (MQ=255)
                                                               aCGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATtaata                                                                                <  1:826271/149‑1 (MQ=255)
                                                               aCGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGAGGGGCGAAATCGATGCAATGGAATTCCTCATtaata                                                                                >  2:319237/1‑149 (MQ=255)
                                                                 gcgTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATACCTCATTAATaaa                                                                              >  2:656350/1‑149 (MQ=255)
                                                                     cTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAACGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTgg                                                                          <  1:1163963/149‑1 (MQ=255)
                                                                       tCCCGGCTATCGACTACAACCGTTCTGGTACACGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAACATCATTCACCCGATGTGCTAAATCGATGCAATGGAATTCATCATTAATAAACTGGCa                                                                        >  1:248039/1‑149 (MQ=255)
                                                                        cccGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGCAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCaa                                                                       >  2:1285748/1‑149 (MQ=255)
                                                                         ccGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAAt                                                                      <  1:924043/149‑1 (MQ=255)
                                                                          cGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAATATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATg                                                                     <  1:880895/149‑1 (MQ=255)
                                                                            gCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgac                                                                   <  1:750420/149‑1 (MQ=255)
                                                                                tCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgaccaag                                                               >  2:1502244/1‑149 (MQ=255)
                                                                                  gACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATgaccaagac                                                             >  1:1364280/1‑149 (MQ=255)
                                                                                        aaCCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAATATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGa                                                       >  1:1106026/1‑149 (MQ=255)
                                                                                        aaCCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGa                                                       >  2:385706/1‑149 (MQ=255)
                                                                                        aaCCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGa                                                       >  2:1402890/1‑149 (MQ=255)
                                                                                         aCCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCATCATTAATAAACTGGCAATGACCAAGACCAATGAc                                                      >  2:202134/1‑149 (MQ=255)
                                                                                            gTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGAt                                                   >  2:1177242/1‑149 (MQ=255)
                                                                                                 ggTACCCGTAAAGAAGAGCTGCTCACGCCTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGACAGCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGCCCAAGCCCAATGACGATttctt                                              >  1:212200/1‑149 (MQ=255)
                                                                                                 ggTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATttctt                                              <  2:610713/149‑1 (MQ=255)
                                                                                                 ggTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATttctt                                              <  1:274484/149‑1 (MQ=255)
                                                                                                 ggTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATttctt                                              <  1:769613/149‑1 (MQ=255)
                                                                                                  gTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGTAATGACCAAGACCAATGACGATttcttc                                             >  2:585708/1‑149 (MQ=255)
                                                                                                  gTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATttcttc                                             <  1:1539722/149‑1 (MQ=255)
                                                                                                  gTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATttcttc                                             >  1:172804/1‑149 (MQ=255)
                                                                                                  gTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATttcttc                                             <  1:633105/149‑1 (MQ=255)
                                                                                                       cGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAat                                        >  1:164186/1‑149 (MQ=255)
                                                                                                        gTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatg                                       >  1:782601/1‑149 (MQ=255)
                                                                                                         tAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAATATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatga                                      <  1:592570/149‑1 (MQ=255)
                                                                                                         tAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAATATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatga                                      <  2:345717/149‑1 (MQ=255)
                                                                                                         tAAACAAGAGCTTCACACGACTCATGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatga                                      <  1:593422/149‑1 (MQ=255)
                                                                                                           aagaagAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAatgatg                                    >  2:1442229/1‑149 (MQ=255)
                                                                                                            agaagaGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAAtgatga                                   >  1:650942/1‑149 (MQ=255)
                                                                                                             taagaGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGaa                                  <  2:1246569/148‑1 (MQ=255)
                                                                                                             gaagaGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGaa                                  >  2:474016/1‑149 (MQ=255)
                                                                                                               agagCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAAc                                <  1:134941/149‑1 (MQ=255)
                                                                                                                 agCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGc                              <  1:100749/149‑1 (MQ=255)
                                                                                                                      ctCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATa                         >  1:1296958/1‑149 (MQ=255)
                                                                                                                       tCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATaa                        >  1:1596945/1‑149 (MQ=255)
                                                                                                                         aCGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAATTGATGAAACGCTCATAAAt                      <  1:820406/149‑1 (MQ=255)
                                                                                                                         aCGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAAt                      >  2:247025/1‑149 (MQ=255)
                                                                                                                           gACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAAttt                    >  1:502738/1‑149 (MQ=255)
                                                                                                                             cTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGt                  <  2:1296958/149‑1 (MQ=255)
                                                                                                                             atCAGGAAGAACTTCAGAAAATGTGGATACTGCGCAAAATCATTCAGCCGATGGGCTAAATCTATGCAATGGAATTCCTCATTAATAAACTGGCTATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGt                  <  1:208265/148‑1 (MQ=255)
                                                                                                                             atCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGt                  <  1:1098683/148‑1 (MQ=255)
                                                                                                                              tCAGGAAGAACTGCAGAATATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTc                 <  2:1106026/149‑1 (MQ=255)
                                                                                                                              tCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATTGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTc                 <  1:26981/149‑1 (MQ=255)
                                                                                                                              tCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTc                 <  1:173692/149‑1 (MQ=255)
                                                                                                                               cAGGAAGAACTGCAGAAAATGTGGATCCAGCGCAAAATCATTCACCCGATGGGCGTAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCACTGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCt                <  2:1103922/149‑1 (MQ=255)
                                                                                                                                aGGAAGAAATGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGAGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGCCCAAGACCAATGACGATTTCTTCGAAATGATGATACGCTCATAAATTTGTCtt               >  1:1254108/1‑149 (MQ=255)
                                                                                                                                 ggAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTa              <  2:1401000/149‑1 (MQ=255)
                                                                                                                                 ggAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTa              <  1:85327/149‑1 (MQ=255)
                                                                                                                                 ggAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTa              >  1:224882/1‑149 (MQ=255)
                                                                                                                                  gaagaaCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAAATGGCAATGCCCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTAt             >  1:988681/1‑149 (MQ=255)
                                                                                                                                   aagaaCTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATCAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATTAATTTGTCTTATg            >  2:1503309/1‑149 (MQ=255)
                                                                                                                                         tGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCaaaa      >  1:968659/1‑149 (MQ=255)
                                                                                                                                         tGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCaaaa      >  1:242867/1‑149 (MQ=255)
                                                                                                                                             gAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGATACGCTCATAAATTTGTCTTATGCCAAAAACGc  >  1:282937/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGC  >  NC_000913/3967409‑3967698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: