Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,007,487 C→T 5.7% L542L (CTG→TTG)  recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,007,4870CT 5.7% 279.8 / 2.1 86L542L (CTG→TTG) recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (37/44);  new base T (2/3);  total (39/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACG  >  NC_000913/4007339‑4007632
                                                                                                                                                    |                                                                                                                                                 
aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAAc                                                                                                                                                   <  2:934862/149‑1 (MQ=255)
aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGAAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAAc                                                                                                                                                   <  1:105275/149‑1 (MQ=255)
 cAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACt                                                                                                                                                  <  2:76775/149‑1 (MQ=255)
 cAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACt                                                                                                                                                  <  1:372368/149‑1 (MQ=255)
    cTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGAAAACTGCg                                                                                                                                               >  1:206191/1‑149 (MQ=255)
     tGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGt                                                                                                                                              <  2:1278103/149‑1 (MQ=255)
     tGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGt                                                                                                                                              >  1:920968/1‑149 (MQ=255)
       aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTaa                                                                                                                                            >  2:1002235/1‑149 (MQ=255)
         cgagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAAt                                                                                                                                          <  1:1552734/148‑1 (MQ=255)
         agagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGCTCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAAt                                                                                                                                          >  2:643556/1‑149 (MQ=255)
         agagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAAt                                                                                                                                          <  1:1487845/149‑1 (MQ=255)
           cggCCGCGCGCCCGGTGCTGCGCGGCGTATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAATCCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAGTTTGCGTaatttg                                                                                                                                        <  2:634629/148‑5 (MQ=255)
            ggCCGCGCGCCCGGTGCTTCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAGTTTGCGTaatttgg                                                                                                                                       <  2:334018/149‑6 (MQ=255)
            ggCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGAGCGCAAACTGTTCGCCAAATTACGCAGTTTGCGTaatttgg                                                                                                                                       <  1:279179/149‑6 (MQ=255)
              ccGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATa                                                                                                                                     <  2:1565126/149‑1 (MQ=255)
                    gcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGAt                                                                                                                               <  1:908041/149‑1 (MQ=255)
                       cGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATCCAGAAATCGTTCGGCGGGAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGaa                                                                                                                            >  1:676636/1‑149 (MQ=255)
                             tgcGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAAt                                                                                                                      <  2:5080/149‑1 (MQ=255)
                                  ggcgAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCATAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTccc                                                                                                                 <  2:821029/149‑1 (MQ=255)
                                    cgAATCCTGTTTGCAACTTGACGTGCCGCGTAGCTTGGAGCTCAAACCGAAAGCTATGCAGAAATCGTTCGGCGGCAACTATGATAGCAAACTGTTCGACAAATTACGCAAACTTATTAAATAGATAGCCGATGAAATTAATGTCccgc                                                                                                               <  2:333921/149‑1 (MQ=255)
                                      aaTCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCccgccg                                                                                                             <  2:644505/149‑1 (MQ=255)
                                        tCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTa                                                                                                           >  2:1134743/1‑149 (MQ=255)
                                          ctctTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACg                                                                                                         <  1:206237/149‑1 (MQ=255)
                                          ctctTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACg                                                                                                         >  1:1356974/1‑149 (MQ=255)
                                          ctctTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGAGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACg                                                                                                         >  2:574400/1‑149 (MQ=255)
                                            ctTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCTAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACgtg                                                                                                       <  1:654648/149‑1 (MQ=255)
                                            ctTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACgtg                                                                                                       <  2:339775/149‑1 (MQ=255)
                                            ctTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACgtg                                                                                                       <  2:458146/149‑1 (MQ=255)
                                            ctTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACgtg                                                                                                       <  2:682677/149‑1 (MQ=255)
                                               tGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCGGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACgtggtg                                                                                                    <  1:959074/149‑1 (MQ=255)
                                                gCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGgtgt                                                                                                   <  2:1558906/149‑1 (MQ=255)
                                                gCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGgtgt                                                                                                   <  2:1330639/149‑1 (MQ=255)
                                                 cAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGAAATGTCCCGCCGTACGTGGTGtt                                                                                                  <  1:1047771/149‑1 (MQ=255)
                                                 cAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTACGCCAAATTACGCAAACTGCGTATATCGATAGCCGATCAAAGTAATGTCCCGCCGTACCTGGTGtt                                                                                                  >  1:268318/1‑149 (MQ=255)
                                                    cTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTaa                                                                                               <  1:532422/149‑1 (MQ=255)
                                                     ttGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAac                                                                                              >  1:1507459/1‑149 (MQ=255)
                                                       gccgtgccgCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCATAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAacga                                                                                            <  2:1122769/149‑1 (MQ=255)
                                                        ccgtgccgCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAacgac                                                                                           <  2:1551442/149‑1 (MQ=255)
                                                          gtgccgCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGc                                                                                         >  1:357386/1‑149 (MQ=255)
                                                          gtgccgCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGAAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATTAAAGTAATGTCCCGCCGTACGTGGGGTTTAACGACGc                                                                                         <  2:1545930/149‑1 (MQ=255)
                                                                 gTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCttg                                                                                  <  1:696753/149‑1 (MQ=255)
                                                                  tATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCttga                                                                                 <  1:742945/149‑1 (MQ=255)
                                                                   aTCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCttgat                                                                                <  1:748365/149‑1 (MQ=255)
                                                                          cgcTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATg                                                                         <  1:608684/149‑1 (MQ=255)
                                                                            cTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCTATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGc                                                                       <  1:1084248/149‑1 (MQ=255)
                                                                             tCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCt                                                                      <  2:357386/149‑1 (MQ=255)
                                                                              cAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTg                                                                     >  2:1188007/1‑149 (MQ=255)
                                                                               aaaCCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGa                                                                    >  2:1561796/1‑149 (MQ=255)
                                                                                aaCCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGaa                                                                   <  2:619465/149‑1 (MQ=255)
                                                                                aaCCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGaa                                                                   <  2:923163/149‑1 (MQ=255)
                                                                                  ccGACAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACa                                                                 <  2:245160/149‑1 (MQ=255)
                                                                                   cGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGTAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAg                                                                >  1:1066405/1‑149 (MQ=255)
                                                                                     aaaGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGAt                                                              >  1:1382672/1‑149 (MQ=255)
                                                                                     aaaGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGAt                                                              >  2:1084409/1‑149 (MQ=255)
                                                                                               aGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCACGCCGTACGTTGTGTTTAACGACGCAACCTTGATTGAGATGGCTGACCAGATGCCGATCAcc                                                    >  1:352190/1‑149 (MQ=255)
                                                                                                gAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCg                                                   >  1:531011/1‑149 (MQ=255)
                                                                                                gAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCg                                                   >  1:671593/1‑149 (MQ=255)
                                                                                                 aaaTCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGc                                                  >  1:561563/1‑149 (MQ=255)
                                                                                                 aaaTCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGc                                                  <  1:1002235/149‑1 (MQ=255)
                                                                                                  aaTCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGcc                                                 >  1:388239/1‑149 (MQ=255)
                                                                                                  aaTCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGcc                                                 >  2:1511386/1‑149 (MQ=255)
                                                                                                   aTCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAAcc                                                                                     <  2:43227/112‑1 (MQ=255)
                                                                                                   aTCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAAcc                                                                                     >  1:43227/1‑112 (MQ=255)
                                                                                                      gTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCg                                             >  1:1353948/1‑149 (MQ=255)
                                                                                                          ggcggcAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAAt                                         >  1:1349917/1‑149 (MQ=255)
                                                                                                           gcggcAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATg                                        >  1:106971/1‑149 (MQ=255)
                                                                                                                 aCTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGc                                  <  2:920968/149‑1 (MQ=255)
                                                                                                                  cTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCAGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCg                                 >  1:955976/1‑149 (MQ=255)
                                                                                                                   tATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGTAATGTCCAGCCGTACGTGGTGTTTAACGACGCAACCTTTATAGAGATTGCTGAAAAGATTCCGATCACAGCCAGCGAAATGATCCGCGt                                >  2:1418728/1‑149 (MQ=255)
                                                                                                                   tATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGt                                >  1:646336/1‑149 (MQ=255)
                                                                                                                   tATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGt                                <  2:1368503/149‑1 (MQ=255)
                                                                                                                       aTCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAAc                            >  1:844149/1‑149 (MQ=255)
                                                                                                                        tCGCAAACTGATCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGACGTACGTGGTGTTTAACGAAGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACAGCCAGCGAAATGCTCAGCGTAAACg                           >  1:261226/1‑149 (MQ=255)
                                                                                                                         cGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACgg                          >  1:533122/1‑149 (MQ=255)
                                                                                                                               cTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTgg                    <  2:671593/149‑1 (MQ=255)
                                                                                                                               cTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTgg                    <  2:55320/149‑1 (MQ=255)
                                                                                                                                tGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTACCGGCGTTggg                   >  1:751829/1‑149 (MQ=255)
                                                                                                                                  ttCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGAt                                                              >  2:1388097/1‑104 (MQ=255)
                                                                                                                                  ttCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGAt                                                              <  1:1388097/104‑1 (MQ=255)
                                                                                                                                  ttCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGAt                 >  2:188844/1‑149 (MQ=255)
                                                                                                                                      ccAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATgcgc             >  1:1408836/1‑149 (MQ=255)
                                                                                                                                         atttACGCAGTTTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAg          >  1:1126845/3‑149 (MQ=255)
                                                                                                                                         aaTTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAg          <  1:1199477/149‑1 (MQ=255)
                                                                                                                                          tttACGCAGTTTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGc         >  1:552204/2‑149 (MQ=255)
                                                                                                                                          aTTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGc         <  2:1080279/149‑1 (MQ=255)
                                                                                                                                          aTTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGc         <  1:657846/149‑1 (MQ=255)
                                                                                                                                               ccAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGaa    <  1:334357/148‑1 (MQ=255)
                                                                                                                                                 aaaCTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACg  >  2:382993/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGACGCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACG  >  NC_000913/4007339‑4007632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: