Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,041,216 T→C 6.3% Q73R (CAG→CGG) ‡ mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,041,2160TC 6.3% 336.1 / 9.4 111Q73R (CAG→CGG) ‡mobBmolybdopterin‑guanine dinucleotide biosynthesis protein B
Reads supporting (aligned to +/- strand):  ref base T (58/46);  new base C (5/2);  total (63/48)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

Only 100 of 113 total aligned reads displayed.

AACAGCACAATCTTTGCGATCTCTTCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAA  >  NC_000913/4041072‑4041364
                                                                                                                                                |                                                                                                                                                    
aaCAGCACAATCTTTGCGATCTCTTCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTgctggct                                                                                                                                                  >  2:401607/1‑149 (MQ=255)
         aTCTTTGCGATCTCTTCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGAt                                                                                                                                         >  2:1222842/1‑149 (MQ=255)
          tCTTTGCGATCTCTTCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCCAGCCAGCAACGATg                                                                                                                                        <  1:712050/149‑1 (MQ=255)
           cTTTGCGATCTCTTCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCCAGCCAGCAACGATgg                                                                                                                                       <  2:508840/149‑1 (MQ=255)
                cGATCTCTTCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTtgt                                                                                                                                  <  1:1341764/149‑1 (MQ=255)
                 gagctctTCATGCTTAAACCCTTCGCCCAGAATCAAAACCAGCTTTGAGGTATCCATTCGCCTTGCGAGAAACTGTAGATGCAGCAGTTCTTGGTCTGGTGTGTCCGGCATCAAGGCCCATCGTTGCTGGCTGGCAACGAGGGGTtggg                                                                                                                                 >  2:54331/4‑147 (MQ=255)
                 gATCTCTTCATGCTTAAACCCTTCGCCCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGATACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGGCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTtgtg                                                                                                                                 >  2:270993/1‑149 (MQ=255)
                    ctctTCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGGCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCcg                                                                                                                              >  1:1576998/1‑149 (MQ=255)
                       ttCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCgcgc                                                                                                                           >  1:1397434/1‑149 (MQ=255)
                       ttCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCgcgc                                                                                                                           >  1:807849/1‑149 (MQ=255)
                       ttCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCgcgc                                                                                                                           >  1:976008/1‑149 (MQ=255)
                       ttCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCgcgc                                                                                                                           >  2:705299/1‑149 (MQ=255)
                        tCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGcc                                                                                                                          >  2:1502943/1‑149 (MQ=255)
                         cATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCa                                                                                                                         >  1:334434/1‑149 (MQ=255)
                           tGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGc                                                                                                                       <  1:526091/149‑1 (MQ=255)
                           tGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACATGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCTTTGCTGGCTGCCAACGATGGTTATTGCCGCGCCAGc                                                                                                                       >  1:819555/1‑149 (MQ=255)
                            gCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGcc                                                                                                                      >  2:1125412/1‑149 (MQ=255)
                             cTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCt                                                                                                                     >  1:598490/1‑149 (MQ=255)
                                aaaCCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGACTGGTGTTTCCGTCATCAAGGCCCATCGTTTCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCttcc                                                                                                                  >  2:456879/1‑147 (MQ=255)
                                    ccTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAg                                                                                                              >  1:439480/1‑149 (MQ=255)
                                     cTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGc                                                                                                             <  1:1310917/149‑1 (MQ=255)
                                     cTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGc                                                                                                             <  1:1125412/149‑1 (MQ=255)
                                     attCGACCAGAATCAAATCCAGCTTTGAGGTATAAATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTACGTCATCAAGGCCCATCGTAGCTGGCTGGCAACGATGGTTTTTGCAGCGCCAGCCTTTCGCAGc                                                                                                             >  2:346273/2‑149 (MQ=255)
                                          aCCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATa                                                                                                        >  2:1322151/1‑149 (MQ=255)
                                           ccAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCGCTTCTTCGTCTGGGGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAg                                                                                                       <  2:1048481/149‑1 (MQ=255)
                                            cAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACTATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGc                                                                                                      >  2:291462/1‑149 (MQ=255)
                                            cAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGc                                                                                                      <  2:1472026/149‑1 (MQ=255)
                                             aGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCt                                                                                                     <  2:1369887/149‑1 (MQ=255)
                                              gAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTa                                                                                                    >  2:931412/1‑149 (MQ=255)
                                               aaTCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTATTCTTCGTCTGGTGTTTACGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGTTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAACTAt                                                                                                   >  2:308807/1‑149 (MQ=255)
                                                 tCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCt                                                                                                 >  1:1062868/1‑149 (MQ=255)
                                                 tCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCACATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCt                                                                                                 >  2:490482/1‑149 (MQ=255)
                                                     aTCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGc                                                                                             <  2:55615/149‑1 (MQ=255)
                                                       ccAGCGTGGAGGGATCCATGCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCGGGTGTTGCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCt                                                                                           <  1:273591/149‑1 (MQ=255)
                                                        cAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTg                                                                                          >  1:193250/1‑149 (MQ=255)
                                                         aGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTgg                                                                                         >  1:205178/1‑149 (MQ=255)
                                                          gCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGc                                                                                        >  2:615628/1‑149 (MQ=255)
                                                            tttGAGGTATCCATTAGACTTGCGAGAAACTGTAGATACAGCTCTTCTTCGTCTGGTGTTTCCGTCATAAAGGCCCATAGTTGCTGGCTTGCAAAGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGATATCTTTTCCTGGCtt                                                                                      >  2:464856/1‑149 (MQ=255)
                                                              tGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTAt                                                                                    <  2:246941/149‑1 (MQ=255)
                                                               gAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATc                                                                                   <  2:1208191/149‑1 (MQ=255)
                                                               gAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCGGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCGGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATc                                                                                   <  1:1427558/149‑1 (MQ=255)
                                                                  gTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCCTCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCacc                                                                                >  1:219363/1‑147 (MQ=255)
                                                                  gTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAAc                                                                                >  1:695855/1‑149 (MQ=255)
                                                                  gTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAAc                                                                                >  2:1296663/1‑149 (MQ=255)
                                                                    aTCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACAt                                                                              >  1:1334219/1‑149 (MQ=255)
                                                                     tCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATc                                                                             >  2:88439/1‑149 (MQ=255)
                                                                      ccATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCATGGCTTATCAACATcc                                                                            >  1:1145175/1‑149 (MQ=255)
                                                                        aTTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCat                                                                          >  2:454018/1‑149 (MQ=255)
                                                                        aTTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCat                                                                          <  1:543287/149‑1 (MQ=255)
                                                                         ttCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGATATCTTTGCCTGGCTTATCAACATCCata                                                                         >  2:671097/1‑149 (MQ=255)
                                                                           cGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATc                                                                       <  1:1502943/149‑1 (MQ=255)
                                                                            gACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCa                                                                      >  1:1318321/1‑149 (MQ=255)
                                                                             aCTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCAATCTTTGCCTGGCTTATCAACATCCATATCAt                                                                     >  1:479830/1‑149 (MQ=255)
                                                                              cTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATg                                                                    <  2:1062868/149‑1 (MQ=255)
                                                                              cTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCATGGCTTATAAACATCCATATCATg                                                                    >  2:968792/1‑149 (MQ=255)
                                                                              cTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCAGAGCTATCTTTGCCTGGGTTATCAACATCCATATCATg                                                                    >  2:265905/1‑149 (MQ=255)
                                                                                   gagaAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCg                                                               >  1:399235/1‑149 (MQ=255)
                                                                                    agaAACTGTAGATCCAGCTCTTCTTCGGCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCACCATCCATATCATGGTGCGt                                                              >  2:1241721/1‑149 (MQ=255)
                                                                                     taaaCTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTa                                                             <  2:1251454/148‑1 (MQ=255)
                                                                                     gaAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTa                                                             <  1:805387/149‑1 (MQ=255)
                                                                                       aaCTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATAGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGTTGCGTATg                                                           >  1:559701/1‑149 (MQ=255)
                                                                                        aCTGTAGATCCAGCGCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCAGCGCCAGCCTTTCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTAttc                                                          <  2:48429/149‑3 (MQ=255)
                                                                                          tGTAGATCCAGATCTTCTTCGTCTGGTTTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATTGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCtt                                                        <  1:456879/149‑1 (MQ=255)
                                                                                           ttAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTa                                                       <  1:884909/148‑1 (MQ=255)
                                                                                           gTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTa                                                       >  2:575911/1‑149 (MQ=255)
                                                                                           gTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTa                                                       >  2:879502/1‑149 (MQ=255)
                                                                                           gTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTa                                                       <  1:270993/149‑1 (MQ=255)
                                                                                               aTCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCa                                                   >  2:49103/1‑149 (MQ=255)
                                                                                                tCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAg                                                  <  2:976008/149‑1 (MQ=255)
                                                                                                    gCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCt                                              >  1:624698/1‑149 (MQ=255)
                                                                                                          cttcGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGc                                                          >  1:1134008/1‑131 (MQ=255)
                                                                                                          cttcGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGc                                                          <  2:1134008/131‑1 (MQ=255)
                                                                                                           ttcGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGACAGCCTTTCGCAGCTAAAACATATCTTTGCCTGGCTTATCAACATCCATATAAAGGTGCGTATGCTTAATCAGCCCTGTACGGa                                       >  1:972114/1‑149 (MQ=255)
                                                                                                             cGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATc                                     <  2:60954/149‑1 (MQ=255)
                                                                                                                   gtgtTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTg                               <  2:1515490/149‑1 (MQ=255)
                                                                                                                     gtTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGgc                             <  1:401607/149‑1 (MQ=255)
                                                                                                                      tttCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGgcg                            >  2:1483023/1‑149 (MQ=255)
                                                                                                                            tcatcaAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCGCATAGCTATCTTTGCCTGGCTTTTCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAAt                      <  1:971301/149‑1 (MQ=255)
                                                                                                                               tcaAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTGGCGCAGCTCATAGCTATCTTTGCCTGGCTTTTCAACATCCATATCATGGTGCGTATGCTTATTCAGCCCTGGTCGGATCCCTCTGGCGCATAATggc                   >  1:252828/1‑147 (MQ=255)
                                                                                                                                caAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCg                  <  1:264514/149‑1 (MQ=255)
                                                                                                                                caAGGCCCATCGTTGCTGGCTGGAAACGATGGTTTGTGCCGCGCGAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATTGTGCGTACGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCg                  <  2:819555/149‑1 (MQ=255)
                                                                                                                                 aaGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCgg                 <  2:1576998/149‑1 (MQ=255)
                                                                                                                                 aaGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCgg                 <  2:652391/149‑1 (MQ=255)
                                                                                                                                    gCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATcc                                    <  1:1322443/127‑1 (MQ=255)
                                                                                                                                    gCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATcc                                    >  2:1322443/1‑127 (MQ=255)
                                                                                                                                     cccATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATAAATATCATGGTGCGTATGCTTAATCAGACCTGGACGGATCCCTCTGGCGCAGAATGCCGGGATc             >  1:1353929/1‑149 (MQ=255)
                                                                                                                                      ccATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCa            <  1:437549/149‑1 (MQ=255)
                                                                                                                                      ccATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCa            >  2:361245/1‑149 (MQ=255)
                                                                                                                                      ccATCGTTGCCAGCCAGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCa            >  2:1370880/1‑149 (MQ=255)
                                                                                                                                       cATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAg           >  1:1564327/1‑149 (MQ=255)
                                                                                                                                       cATCGTTGCCAGCCAGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCGCTGGACGGATCCCTCTGGCGCATAATGCAGGGATCat           >  1:415322/1‑148 (MQ=255)
                                                                                                                                       cATCGTTGCCAGCCAGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAg           >  2:891381/1‑149 (MQ=255)
                                                                                                                                       cATCGTTGCCAGCCAGCAACGATGGTATGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTAAGCTTTATCAGCCCTGGACGGATCCCTCTGTCGCATAATGCCGGGCTCAg           >  2:151189/1‑149 (MQ=255)
                                                                                                                                        aTCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGt          <  1:668774/149‑1 (MQ=255)
                                                                                                                                         tCGTTGCCAGCCAGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGtt         >  1:889969/1‑149 (MQ=255)
                                                                                                                                           gTTGCTGGCTGGCAACGATGGTTTGGTCCGCGCCAGCCTTGCGCAGCTCATATCTATCTTTGCCTGTCTTATCCACATCCATATCATGGTGCGTATGCTTAATCAGAACTGGACGGATCACTCTGGCGCATAATGCCGGGATCAGtttt       <  1:869335/149‑1 (MQ=255)
                                                                                                                                             tGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGtttttt     <  1:746110/149‑1 (MQ=255)
                                                                                                                                             tGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGtttttt     <  1:583091/149‑1 (MQ=255)
                                                                                                                                             tGCTGGCTGGCAACGATGGGTTGTGGCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTAAGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGtttttt     <  2:127492/149‑1 (MQ=255)
                                                                                                                                                tggctggcAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCaa  >  1:1583778/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AACAGCACAATCTTTGCGATCTCTTCATGCTTAAACCCTTCGACCAGAATCAAATCCAGCTTTGAGGTATCCATTCGACTTGCGAGAAACTGTAGATCCAGCTCTTCTTCGTCTGGTGTTTCCGTCATCAAGGCCCATCGTTGCTGGCTGGCAACGATGGTTTGTGCCGCGCCAGCCTTGCGCAGCTCATAGCTATCTTTGCCTGGCTTATCAACATCCATATCATGGTGCGTATGCTTAATCAGCCCTGGACGGATCCCTCTGGCGCATAATGCCGGGATCAGTTTTTTCAA  >  NC_000913/4041072‑4041364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: