Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,092,795 T→G 9.2% D10A (GAC→GCC)  frvA ← putative enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,092,7950TG 9.2% 303.3 / 14.7 103D10A (GAC→GCC) frvAputative enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base T (51/42);  new base G (7/3);  total (58/45)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

ACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGG  >  NC_000913/4092647‑4092942
                                                                                                                                                     |                                                                                                                                                     
acCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGt                                                                                                                                                       >  1:1536721/1‑149 (MQ=255)
  cGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTGCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCa                                                                                                                                                     >  2:970571/1‑149 (MQ=255)
   ggAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCaa                                                                                                                                                    <  1:938572/149‑1 (MQ=255)
    gAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTGTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAAt                                                                                                                                                   >  1:699111/1‑149 (MQ=255)
     aaCCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATa                                                                                                                                                  <  2:223336/149‑1 (MQ=255)
           aTCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCtt                                                                                                                                            >  1:1360559/1‑149 (MQ=255)
            tCCAGTAGAGTGCATTTTTTCGAGCAGCAGCAGTGTTTGCATGAACTGGTGTGAGTCTGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTg                                                                                                                                           <  1:996352/149‑1 (MQ=255)
              cAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCa                                                                                                                                         >  2:1301732/1‑149 (MQ=255)
                gttgagTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGGTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTTCAGt                                                                                                                                       <  1:81223/146‑1 (MQ=255)
                 tAGAGTGCATTTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGt                                                                                                                                       <  1:772877/149‑1 (MQ=255)
                      tGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGATCAATACAGCTTGCAGTAAGAGc                                                                                                                                 >  1:133474/1‑149 (MQ=255)
                        cATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTg                                                                                                                               >  1:7306/1‑149 (MQ=255)
                           ttttttCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGATCGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGcc                                                                                                                             >  1:1562656/1‑149 (MQ=255)
                           ttttttCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGcc                                                                                                                             >  1:338074/1‑149 (MQ=255)
                            tttttCGCGCAGCAGCAGGGGTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATAGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCa                                                                                                                            <  2:159352/149‑1 (MQ=255)
                                  gcgcAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGCGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATc                                                                                                                      >  1:714855/1‑149 (MQ=255)
                                   cgcAGCAGCAGGGTTTGCAGGAACTGGGGTGAGGCGGTGATAAACCCGTTAGGTAACGCTATTGACGCCACCTTCTTCAGACCGGAATAAGCGCCATTGCCCTGAATATTCAGGGCACTACAGCTTGCAGTAAGAGCTGCCATTAATCg                                                                                                                     <  1:230209/149‑1 (MQ=255)
                                     cagcagcagGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGa                                                                                                                   <  1:1472806/149‑1 (MQ=255)
                                         agcagGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTcc                                                                                                               <  2:1396033/149‑1 (MQ=255)
                                         agcagGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTcc                                                                                                               >  2:653915/1‑149 (MQ=255)
                                            agGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATa                                                                                                            >  1:659755/1‑149 (MQ=255)
                                               gTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATaaa‑‑a                                                                                                       <  2:89384/149‑1 (MQ=255)
                                               gTTGGCAGGAACTGGTGTGAGTCGGGGATAAAACCGTGTTGTAACGCTCTTGGCGCCAACTGCTTCAGAACGGACTATGCTCCTTTGCCCTTATTATTCAGCTCAATACAGCGTGCAGTAAGTGCTGCCATTAATCGGACTCCATaaa‑‑a                                                                                                       <  2:258252/149‑1 (MQ=255)
                                                   gCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑atata                                                                                                   <  1:1237600/149‑1 (MQ=255)
                                                     aGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATAtt                                                                                                 <  1:1422242/149‑1 (MQ=255)
                                                          cTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAAt                                                                                            <  2:553664/149‑1 (MQ=255)
                                                            ggTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTatgga                                                                                          <  2:1166184/149‑5 (MQ=255)
                                                            ggTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTatgga                                                                                          <  2:1436559/149‑5 (MQ=255)
                                                             gtgtGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCaa                                                                                         <  1:1527442/149‑1 (MQ=255)
                                                             gtgtGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCaa                                                                                         >  2:1527442/1‑149 (MQ=255)
                                                              tgtgAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAAt                                                                                        <  2:402713/149‑1 (MQ=255)
                                                                 gAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGt                                                                                     >  1:1188682/1‑149 (MQ=255)
                                                                  aGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTa                                                                                    >  2:1100191/1‑149 (MQ=255)
                                                                    tCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAAt                                                                                  <  1:1086883/149‑1 (MQ=255)
                                                                    tCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAAt                                                                                  >  1:1366861/1‑149 (MQ=255)
                                                                     cGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAAtg                                                                                 <  1:1183556/149‑1 (MQ=255)
                                                                         gATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAAtggtgg                                                                             <  2:581757/149‑1 (MQ=255)
                                                                         gATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAAtggtgg                                                                             <  2:18972/149‑1 (MQ=255)
                                                                          aTAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGa                                                                            >  2:349008/1‑149 (MQ=255)
                                                                             aaaCCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCg                                                                         >  2:332059/1‑149 (MQ=255)
                                                                              aaCCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGa                                                                        >  1:826103/1‑149 (MQ=255)
                                                                               aCCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGAt                                                                       >  1:280473/1‑149 (MQ=255)
                                                                                ccGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGAtt                                                                      >  1:934664/1‑149 (MQ=255)
                                                                                 cGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTa                                                                     <  1:332059/149‑1 (MQ=255)
                                                                                   ttttGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAg                                                                   >  1:1144202/1‑149 (MQ=255)
                                                                                    tttGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGt                                                                  <  1:584228/149‑1 (MQ=255)
                                                                                       gTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGtgat                                                               <  2:341881/149‑1 (MQ=255)
                                                                                        tAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGtgatg                                                              <  2:1188682/149‑1 (MQ=255)
                                                                                          aCGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCATGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTAAATCAATTGTAATGGTGGAGCGATTAAGTGATgag                                                            <  2:1037135/149‑1 (MQ=255)
                                                                                           cGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATgaga                                                           >  1:1080678/1‑149 (MQ=255)
                                                                                              tatattCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGGCAATACAGCTTGCAGAATGAGCAGCCATAAATCGGACTCCATAAA‑‑ATATATTTAAATCAAATGTAATGGTGAAGCGATTATCTGATGATAGGc                                                        >  1:74122/6‑149 (MQ=255)
                                                                                              tATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGc                                                        >  1:1073131/1‑149 (MQ=255)
                                                                                                ttGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGa                                                      >  1:1448086/1‑149 (MQ=255)
                                                                                                 tGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGat                                                     <  2:1360559/149‑1 (MQ=255)
                                                                                                 tGTCGCCAACTGCTTCAGAACGGAATAAGAGCCATTGCCCTGAATATTTAGGTCAATACAGCTTGCAGTAAGAGCTGACATTTATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCgtt                                                     <  2:74122/149‑3 (MQ=255)
                                                                                                  gTCGCCAACTGCTTCAGAACGGAATCAGCGCCAATGCCCTGACTATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGa                                                      <  2:141662/149‑1 (MQ=255)
                                                                                                             cTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAAc                                         >  1:807536/1‑149 (MQ=255)
                                                                                                              ttCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTATACa                                        >  1:716059/1‑149 (MQ=255)
                                                                                                              ttCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACa                                        >  1:478587/1‑149 (MQ=255)
                                                                                                                cAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGATCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAAt                                      <  2:133474/149‑1 (MQ=255)
                                                                                                                 aGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATg                                     >  2:398163/1‑149 (MQ=255)
                                                                                                                 aGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATg                                     >  2:29904/1‑149 (MQ=255)
                                                                                                                 aGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATg                                     >  1:433927/1‑149 (MQ=255)
                                                                                                                  taaCGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGa                                    <  1:1038602/148‑1 (MQ=255)
                                                                                                                  gAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGa                                    >  2:152399/1‑149 (MQ=255)
                                                                                                                   aaCGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGAc                                   >  1:980240/1‑149 (MQ=255)
                                                                                                                     cGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCATTTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGaaaa                                 >  2:1386868/1‑149 (MQ=255)
                                                                                                                       gAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAAtt                               <  1:1301732/149‑1 (MQ=255)
                                                                                                                       gAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAAtt                               >  2:172351/1‑149 (MQ=255)
                                                                                                                        aaTAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAAttt                              <  2:1080678/149‑1 (MQ=255)
                                                                                                                        aaTAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAAttt                              <  1:45578/149‑1 (MQ=255)
                                                                                                                         aTAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTg                             >  1:1596906/1‑149 (MQ=255)
                                                                                                                         aTAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTg                             <  1:1514146/149‑1 (MQ=255)
                                                                                                                          tAAGCGCCATTGCCGTGAATATTCAGTTCAATACAGATTTCAGTAAGTGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGAAATGGTGGATCGTTTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTga                            <  2:342556/149‑1 (MQ=255)
                                                                                                                          tAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTga                            <  2:663292/149‑1 (MQ=255)
                                                                                                                          tAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTga                            <  1:1100191/149‑1 (MQ=255)
                                                                                                                          tAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATCACAATTTga                            >  2:1489468/1‑149 (MQ=255)
                                                                                                                            aGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTgaga                          >  2:441320/1‑149 (MQ=255)
                                                                                                                             gcgcCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGAc                         >  2:147702/1‑149 (MQ=255)
                                                                                                                               tgtaTTGACCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACag                       >  1:1059336/4‑149 (MQ=255)
                                                                                                                               tgtaTTGACCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACag                       >  2:1411557/4‑149 (MQ=255)
                                                                                                                                 taTTGACCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGt                                             <  1:1259653/124‑1 (MQ=255)
                                                                                                                                 taTTGACCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGt                                             >  2:1259653/2‑125 (MQ=255)
                                                                                                                                   ttGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTg                   >  2:1517312/1‑149 (MQ=255)
                                                                                                                                   ttGACCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTg                   >  1:1441588/1‑149 (MQ=255)
                                                                                                                                      cccTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGaaa                >  2:986879/1‑149 (MQ=255)
                                                                                                                                      accTGAATATTCAGGGCAATACAGCTTGCTGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGaaa                >  1:241822/2‑149 (MQ=255)
                                                                                                                                      accTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGaaa                >  2:458108/2‑149 (MQ=255)
                                                                                                                                       ccTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAAGCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAg               >  2:1066309/1‑149 (MQ=255)
                                                                                                                                        cTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAgg              >  2:1266661/1‑149 (MQ=255)
                                                                                                                                           aaTATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCa           >  2:193706/1‑149 (MQ=255)
                                                                                                                                           aaTATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCa           <  2:338074/149‑1 (MQ=255)
                                                                                                                                             tatTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGa         <  1:421066/149‑1 (MQ=255)
                                                                                                                                             tatTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGa         <  2:1536721/149‑1 (MQ=255)
                                                                                                                                               ttCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGAtt       >  1:419777/1‑149 (MQ=255)
                                                                                                                                               ttCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGAtt       >  2:800847/1‑149 (MQ=255)
                                                                                                                                               ttCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGAtt       >  2:1528743/1‑149 (MQ=255)
                                                                                                                                                tCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGAAAATTTGAGACAGAGTGAAAGGTCAGAttt      >  1:561441/1‑149 (MQ=255)
                                                                                                                                                  aGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGc    >  1:1425274/1‑149 (MQ=255)
                                                                                                                                                    gTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCgg  >  1:953665/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAA‑‑ATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGG  >  NC_000913/4092647‑4092942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: