Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,103,526 A→G 5.5% intergenic (+30/+76) yiiM → / ← cpxA 6‑N‑hydroxylaminopurine resistance protein/sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,5260AG 5.5% 286.4 / 4.8 91intergenic (+30/+76)yiiM/cpxA6‑N‑hydroxylaminopurine resistance protein/sensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base A (49/37);  new base G (3/2);  total (52/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01

ATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACC  >  NC_000913/4103378‑4103674
                                                                                                                                                     |                                                                                                                                                    
aTCAATATCACCGTTTCCTCTCCGCTGCCGGGTTACCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGGGTAGGCCTGATAAGACGCTa                                                                                                                                                      <  1:290543/149‑1 (MQ=255)
 tCAATATCACCGTTTACTCTCCTCTGCCGGGTTATCGAAAAGCGGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCTAGATCGAAGATTTTTCCCGCCGGTTGAGGGGGAAAATATCCCCCGGTGTGTAGGCCCGATAAGACGCTa                                                                                                                                                      >  1:1350854/1‑149 (MQ=255)
 tCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTAt                                                                                                                                                     >  2:585726/1‑149 (MQ=255)
   aaTATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCa                                                                                                                                                   <  1:744295/149‑1 (MQ=255)
     tatCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGc                                                                                                                                                 <  2:942343/149‑1 (MQ=255)
      atCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCg                                                                                                                                                >  1:1066800/1‑149 (MQ=255)
       tCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGctt                                                                                                                                               >  1:680021/1‑147 (MQ=255)
        cACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTc                                                                                                                                              <  1:21174/149‑1 (MQ=255)
         aCCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCATAAGCGCCGACTGAGCGGCAAGATCGAAGATTATTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCg                                                                                                                                             <  1:262280/149‑1 (MQ=255)
         aCCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCg                                                                                                                                             >  2:536795/1‑149 (MQ=255)
          ccGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGc                                                                                                                                            <  2:413179/149‑1 (MQ=255)
           cGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTGATAGCGTctta                                                                                                                                           <  2:749004/149‑4 (MQ=255)
             tttACTCTACGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCAGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATc                                                                                                                                         >  2:1134272/1‑149 (MQ=255)
              ttACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCa                                                                                                                                        >  1:57101/1‑149 (MQ=255)
               tACTCTCCGCTTCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAg                                                                                                                                       <  2:808741/149‑1 (MQ=255)
               tACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTATGCCTGATGCGACGCTGATAGCGTCTTATCAg                                                                                                                                       <  2:441644/149‑1 (MQ=255)
                 ctctCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGATGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGc                                                                                                                                     >  2:788819/1‑149 (MQ=255)
                  tctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCa                                                                                                                                    >  1:1477218/1‑149 (MQ=255)
                    tcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATc                                                                                                                                  <  1:387975/149‑1 (MQ=255)
                        cTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGc                                                                                                                              >  2:615008/1‑149 (MQ=255)
                          gCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTc                                                                                                                            <  1:585726/149‑1 (MQ=255)
                              ggTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAAt                                                                                                                        >  1:208640/1‑149 (MQ=255)
                                  aTCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCg                                                                                                                    <  2:1494992/149‑1 (MQ=255)
                                   tCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCg                                                                                                                    >  2:60429/1‑148 (MQ=255)
                                   tCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCg                                                                                                                    <  1:60429/148‑1 (MQ=255)
                                     gAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCCGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCAGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGAt                                                                                                                 >  2:931961/1‑149 (MQ=255)
                                      aaaaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATc                                                                                                                                  <  1:403152/131‑1 (MQ=255)
                                      aaaaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATc                                                                                                                                  >  2:403152/1‑131 (MQ=255)
                                        aaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcg                                                                                                              <  2:862932/149‑1 (MQ=255)
                                         aGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcgg                                                                                                             <  1:1204314/149‑1 (MQ=255)
                                         aGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcgg                                                                                                             <  1:1229865/149‑1 (MQ=255)
                                           cTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcggcg                                                                                                           <  1:2845/149‑1 (MQ=255)
                                            tgtaagcgGACGATGAAAAAGCGCAGACTGAGCGGCAAGTTCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCTAATGCCGGATGCGGCGt                                                                                                          <  2:1211046/144‑1 (MQ=39)
                                              gACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGCTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTaa                                                                                                        >  2:1220264/1‑149 (MQ=255)
                                               aCGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTaaa                                                                                                       <  1:1566411/149‑1 (MQ=255)
                                                  cgGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGc                                                                                                    <  1:1354732/149‑1 (MQ=255)
                                                    gACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCt                                                                                                  >  2:570803/1‑149 (MQ=255)
                                                        aTGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATc                                                                                              <  1:874868/149‑1 (MQ=255)
                                                           cAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctg                                                                                           <  1:814902/149‑1 (MQ=255)
                                                            aaaaGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgc                                                                                          >  2:1232099/1‑149 (MQ=255)
                                                             aaaGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgcc                                                                                         >  1:48526/1‑149 (MQ=255)
                                                                 cgcCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCa                                                                                     <  2:89267/149‑1 (MQ=255)
                                                                  gcCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCaa                                                                                    <  1:866630/149‑1 (MQ=255)
                                                                   ccGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCaaa                                                                                   >  1:317064/1‑149 (MQ=255)
                                                                    cGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAAt                                                                                  >  1:544079/1‑149 (MQ=255)
                                                                    cGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAAt                                                                                  >  2:435404/1‑149 (MQ=255)
                                                                     gACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATg                                                                                 >  1:1575306/1‑149 (MQ=255)
                                                                      aCTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGc                                                                                >  2:1049020/1‑149 (MQ=255)
                                                                        tGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCGTATCCTGCCTGCAAATGCGa                                                                              >  1:203201/1‑149 (MQ=255)
                                                                         gAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGaa                                                                             <  1:47681/149‑1 (MQ=255)
                                                                         gAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGaa                                                                             <  2:833768/149‑1 (MQ=255)
                                                                          aGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAg                                                                            >  2:111295/1‑149 (MQ=255)
                                                                          aGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAg                                                                            >  2:150860/1‑149 (MQ=255)
                                                                            cGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGtt                                                                          <  2:298602/149‑1 (MQ=255)
                                                                              gCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTa                                                                        >  1:1163516/1‑149 (MQ=255)
                                                                               cAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                       >  1:1384846/1‑149 (MQ=255)
                                                                               cAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                       <  1:592365/149‑1 (MQ=255)
                                                                               cAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                       >  1:1160202/1‑149 (MQ=255)
                                                                                  gATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTc                                                                    >  2:308038/1‑149 (MQ=255)
                                                                                   aTCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTcc                                                                   >  2:396811/1‑149 (MQ=255)
                                                                                    tCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCg                                                                  >  1:635016/1‑149 (MQ=255)
                                                                                       aaGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAACGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCtt                                                               >  2:1438181/1‑149 (MQ=255)
                                                                                              ttCCCGCCTGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGc                                                        <  1:1134272/149‑1 (MQ=255)
                                                                                                   cccgGTTG‑TTGGGAAAATAACACCCGGATTGTATGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCaa                                                   <  2:255276/148‑1 (MQ=255)
                                                                                                             ggggAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCAc                                          <  2:57101/149‑1 (MQ=255)
                                                                                                               ggAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCa                                        <  1:656573/149‑1 (MQ=255)
                                                                                                                gAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAg                                       >  1:476519/1‑149 (MQ=255)
                                                                                                                     tAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTa                                  >  2:888122/1‑149 (MQ=255)
                                                                                                                       aCCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTaaa                                <  2:1477218/149‑1 (MQ=255)
                                                                                                                        cccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAAc                               >  2:1115936/1‑149 (MQ=255)
                                                                                                                          cccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCGTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTactccg                             >  1:250777/1‑144 (MQ=255)
                                                                                                                          cccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGGTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCg                             >  1:250778/1‑149 (MQ=255)
                                                                                                                                 tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCg                      >  2:754594/1‑149 (MQ=255)
                                                                                                                                   tAGGCCTGAT‑AGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCGGCCGTAAACCGCCCAGCGGGc                   >  1:1361571/1‑149 (MQ=255)
                                                                                                                                   gatgCCTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCggg                    >  2:334011/4‑149 (MQ=38)
                                                                                                                                    atgCCTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGc                   >  1:1281382/3‑149 (MQ=38)
                                                                                                                                     tgCCTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCt                  >  2:1398240/2‑149 (MQ=38)
                                                                                                                                     ggCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCt                  >  1:877562/1‑149 (MQ=255)
                                                                                                                                      gCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCACCCAAATCACCAGCCGTAATCCGCCCAGCGGGCTg                 >  1:509692/1‑149 (MQ=255)
                                                                                                                                      gCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTg                 >  1:834797/1‑149 (MQ=255)
                                                                                                                                       ccTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGt                >  2:1388565/1‑149 (MQ=255)
                                                                                                                                        cTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtc               >  2:188652/1‑149 (MQ=255)
                                                                                                                                        cTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtc               <  1:615008/149‑1 (MQ=255)
                                                                                                                                         tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCTGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct              <  1:788819/149‑1 (MQ=255)
                                                                                                                                         tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct              <  1:527189/149‑1 (MQ=255)
                                                                                                                                         tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct              <  1:920352/149‑1 (MQ=255)
                                                                                                                                         tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct              >  2:1182401/1‑149 (MQ=255)
                                                                                                                                          gATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtctt             >  1:1454720/1‑149 (MQ=255)
                                                                                                                                           aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttc            >  2:508233/1‑149 (MQ=255)
                                                                                                                                            tAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTAAACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttct           <  2:48526/149‑1 (MQ=255)
                                                                                                                                               gACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGcc        >  1:1318388/1‑149 (MQ=255)
                                                                                                                                               gACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGcc        <  1:210714/149‑1 (MQ=255)
                                                                                                                                                    tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAACTCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCAc   >  1:795006/1‑149 (MQ=255)
                                                                                                                                                     aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCAcc  <  2:1575306/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTG‑TGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACC  >  NC_000913/4103378‑4103674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑