Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,103,528 C→T 5.4% intergenic (+32/+74) yiiM → / ← cpxA 6‑N‑hydroxylaminopurine resistance protein/sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,5280CT 5.4% 298.2 / 4.1 93intergenic (+32/+74)yiiM/cpxA6‑N‑hydroxylaminopurine resistance protein/sensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base C (50/38);  new base T (3/2);  total (53/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

AATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCA  >  NC_000913/4103381‑4103676
                                                                                                                                                   |                                                                                                                                                    
aaTATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCa                                                                                                                                                     <  1:744295/149‑1 (MQ=255)
  tatCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGc                                                                                                                                                   <  2:942343/149‑1 (MQ=255)
   atCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCg                                                                                                                                                  >  1:1066800/1‑149 (MQ=255)
    tCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGctt                                                                                                                                                 >  1:680021/1‑147 (MQ=255)
     cACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTc                                                                                                                                                <  1:21174/149‑1 (MQ=255)
      aCCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCATAAGCGCCGACTGAGCGGCAAGATCGAAGATTATTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCg                                                                                                                                               <  1:262280/149‑1 (MQ=255)
      aCCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCg                                                                                                                                               >  2:536795/1‑149 (MQ=255)
       ccGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGc                                                                                                                                              <  2:413179/149‑1 (MQ=255)
        cGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTGATAGCGTctta                                                                                                                                             <  2:749004/149‑4 (MQ=255)
          tttACTCTACGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCAGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATc                                                                                                                                           >  2:1134272/1‑149 (MQ=255)
           ttACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCa                                                                                                                                          >  1:57101/1‑149 (MQ=255)
            tACTCTCCGCTTCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAg                                                                                                                                         <  2:808741/149‑1 (MQ=255)
            tACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTATGCCTGATGCGACGCTGATAGCGTCTTATCAg                                                                                                                                         <  2:441644/149‑1 (MQ=255)
              ctctCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGATGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGc                                                                                                                                       >  2:788819/1‑149 (MQ=255)
               tctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCa                                                                                                                                      >  1:1477218/1‑149 (MQ=255)
                 tcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATc                                                                                                                                    <  1:387975/149‑1 (MQ=255)
                     cTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGc                                                                                                                                >  2:615008/1‑149 (MQ=255)
                       gCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTc                                                                                                                              <  1:585726/149‑1 (MQ=255)
                           ggTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAAt                                                                                                                          >  1:208640/1‑149 (MQ=255)
                               aTCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCg                                                                                                                      <  2:1494992/149‑1 (MQ=255)
                                tCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCg                                                                                                                      <  1:60429/148‑1 (MQ=255)
                                tCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCg                                                                                                                      >  2:60429/1‑148 (MQ=255)
                                  gAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCCGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCAGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGAt                                                                                                                   >  2:931961/1‑149 (MQ=255)
                                   aaaaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATc                                                                                                                                    >  2:403152/1‑131 (MQ=255)
                                   aaaaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATc                                                                                                                                    <  1:403152/131‑1 (MQ=255)
                                     aaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcg                                                                                                                <  2:862932/149‑1 (MQ=255)
                                      aGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcgg                                                                                                               <  1:1229865/149‑1 (MQ=255)
                                      aGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcgg                                                                                                               <  1:1204314/149‑1 (MQ=255)
                                        cTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcggcg                                                                                                             <  1:2845/149‑1 (MQ=255)
                                         tgtaagcgGACGATGAAAAAGCGCAGACTGAGCGGCAAGTTCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCTAATGCCGGATGCGGCGt                                                                                                            <  2:1211046/144‑1 (MQ=39)
                                           gACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGCTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTaa                                                                                                          >  2:1220264/1‑149 (MQ=255)
                                            aCGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTaaa                                                                                                         <  1:1566411/149‑1 (MQ=255)
                                               cgGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGc                                                                                                      <  1:1354732/149‑1 (MQ=255)
                                                 gACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCt                                                                                                    >  2:570803/1‑149 (MQ=255)
                                                     aTGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATc                                                                                                <  1:874868/149‑1 (MQ=255)
                                                        cAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctg                                                                                             <  1:814902/149‑1 (MQ=255)
                                                         aaaaGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgc                                                                                            >  2:1232099/1‑149 (MQ=255)
                                                          aaaGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgcc                                                                                           >  1:48526/1‑149 (MQ=255)
                                                              cgcCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCa                                                                                       <  2:89267/149‑1 (MQ=255)
                                                               gcCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCaa                                                                                      <  1:866630/149‑1 (MQ=255)
                                                                ccGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCaaa                                                                                     >  1:317064/1‑149 (MQ=255)
                                                                 cGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAAt                                                                                    >  1:544079/1‑149 (MQ=255)
                                                                 cGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAAt                                                                                    >  2:435404/1‑149 (MQ=255)
                                                                  gACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATg                                                                                   >  1:1575306/1‑149 (MQ=255)
                                                                   aCTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGc                                                                                  >  2:1049020/1‑149 (MQ=255)
                                                                     tGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCGTATCCTGCCTGCAAATGCGa                                                                                >  1:203201/1‑149 (MQ=255)
                                                                      gAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGaa                                                                               <  2:833768/149‑1 (MQ=255)
                                                                      gAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGaa                                                                               <  1:47681/149‑1 (MQ=255)
                                                                       aGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAg                                                                              >  2:150860/1‑149 (MQ=255)
                                                                       aGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAg                                                                              >  2:111295/1‑149 (MQ=255)
                                                                         cGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGtt                                                                            <  2:298602/149‑1 (MQ=255)
                                                                           gCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTa                                                                          >  1:1163516/1‑149 (MQ=255)
                                                                            cAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                         <  1:592365/149‑1 (MQ=255)
                                                                            cAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                         >  1:1384846/1‑149 (MQ=255)
                                                                            cAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                         >  1:1160202/1‑149 (MQ=255)
                                                                               gATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTc                                                                      >  2:308038/1‑149 (MQ=255)
                                                                                aTCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTcc                                                                     >  2:396811/1‑149 (MQ=255)
                                                                                 tCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCg                                                                    >  1:635016/1‑149 (MQ=255)
                                                                                    aaGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAACGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCtt                                                                 >  2:1438181/1‑149 (MQ=255)
                                                                                           ttCCCGCCTGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGc                                                          <  1:1134272/149‑1 (MQ=255)
                                                                                                cccgGTTGTTGGGAAAATAACACCCGGATTGTATGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCaa                                                     <  2:255276/148‑1 (MQ=255)
                                                                                                         ggggAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCAc                                            <  2:57101/149‑1 (MQ=255)
                                                                                                           ggAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCa                                          <  1:656573/149‑1 (MQ=255)
                                                                                                            gAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAg                                         >  1:476519/1‑149 (MQ=255)
                                                                                                                 tAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTa                                    >  2:888122/1‑149 (MQ=255)
                                                                                                                   aCCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTaaa                                  <  2:1477218/149‑1 (MQ=255)
                                                                                                                    cccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAAc                                 >  2:1115936/1‑149 (MQ=255)
                                                                                                                      cccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCGTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTactccg                               >  1:250777/1‑144 (MQ=255)
                                                                                                                      cccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGGTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCg                               >  1:250778/1‑149 (MQ=255)
                                                                                                                             tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCg                        >  2:754594/1‑149 (MQ=255)
                                                                                                                               tAGGCCTGAT‑AGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCGGCCGTAAACCGCCCAGCGGGc                     >  1:1361571/1‑149 (MQ=255)
                                                                                                                               gatgCCTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCggg                      >  2:334011/4‑149 (MQ=38)
                                                                                                                                atgCCTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGc                     >  1:1281382/3‑149 (MQ=38)
                                                                                                                                 tgCCTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCt                    >  2:1398240/2‑149 (MQ=38)
                                                                                                                                 ggCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCt                    >  1:877562/1‑149 (MQ=255)
                                                                                                                                  gCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCACCCAAATCACCAGCCGTAATCCGCCCAGCGGGCTg                   >  1:509692/1‑149 (MQ=255)
                                                                                                                                  gCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTg                   >  1:834797/1‑149 (MQ=255)
                                                                                                                                   ccTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGt                  >  2:1388565/1‑149 (MQ=255)
                                                                                                                                    cTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtc                 <  1:615008/149‑1 (MQ=255)
                                                                                                                                    cTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtc                 >  2:188652/1‑149 (MQ=255)
                                                                                                                                     tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCTGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct                <  1:788819/149‑1 (MQ=255)
                                                                                                                                     tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct                <  1:920352/149‑1 (MQ=255)
                                                                                                                                     tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct                >  2:1182401/1‑149 (MQ=255)
                                                                                                                                     tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct                <  1:527189/149‑1 (MQ=255)
                                                                                                                                      gATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtctt               >  1:1454720/1‑149 (MQ=255)
                                                                                                                                       aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttc              >  2:508233/1‑149 (MQ=255)
                                                                                                                                        tAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTAAACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttct             <  2:48526/149‑1 (MQ=255)
                                                                                                                                           gACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGcc          <  1:210714/149‑1 (MQ=255)
                                                                                                                                           gACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGcc          >  1:1318388/1‑149 (MQ=255)
                                                                                                                                                tATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAACTCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCAc     >  1:795006/1‑149 (MQ=255)
                                                                                                                                                 aTCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCAcc    <  2:1575306/149‑1 (MQ=255)
                                                                                                                                                  tCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAACTCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCAccc   >  1:827350/1‑149 (MQ=255)
                                                                                                                                                  tCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCAccc   >  2:1213496/1‑149 (MQ=255)
                                                                                                                                                   cAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCa  >  2:1470041/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCCA  >  NC_000913/4103381‑4103676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑