Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 446,789 A→G 8.3% intergenic (‑123/+26) yajR ← / ← cyoE putative transporter/protoheme IX farnesyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913446,7890AG 8.3% 243.7 / 10.2 84intergenic (‑123/+26)yajR/cyoEputative transporter/protoheme IX farnesyltransferase
Reads supporting (aligned to +/- strand):  ref base A (36/41);  new base G (4/3);  total (40/44)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

CCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTC  >  NC_000913/446641‑446937
                                                                                                                                                    |                                                                                                                                                    
ccTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTa                                                                                                                                                      >  2:941812/1‑149 (MQ=255)
 cTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAg                                                                                                                                                     >  1:1179397/1‑149 (MQ=255)
  tGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGc                                                                                                                                                    >  1:1458214/1‑149 (MQ=255)
      gTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTCTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGTTGCCGTAGCACCt                                                                                                                                                >  1:713152/1‑149 (MQ=255)
       tCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCtt                                                                                                                                               <  1:1037877/149‑1 (MQ=255)
       tCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCtt                                                                                                                                               >  2:2576/1‑149 (MQ=255)
        cATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCttt                                                                                                                                              >  1:1355938/1‑149 (MQ=255)
        aaTTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttt                                                                                                                                              <  2:716774/148‑1 (MQ=255)
          ttttATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCttttt                                                                                                                                            >  2:792673/1‑149 (MQ=255)
           tttATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCTGATTTTTTAGTAAATATTTCGAGAGTGATATGTTTCTAATGCTAAGAAAAAATGTGCCGTAGCAACTTTTTa                                                                                                                                           >  1:1222235/1‑149 (MQ=255)
              ataATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATa                                                                                                                                        <  2:1305291/149‑1 (MQ=255)
               taATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATag                                                                                                                                       <  2:1199303/149‑1 (MQ=255)
               aaaTCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttttttctta                                                                                                                                       <  1:1067682/148‑6 (MQ=255)
                 aTCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACC‑TTTTAATagaga                                                                                                                                    <  1:707004/149‑1 (MQ=255)
                   cGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATagagag                                                                                                                                   <  2:625560/149‑1 (MQ=255)
                     ttCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGt                                                                                                                                 >  1:1485374/1‑149 (MQ=255)
                         ttACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTg                                                                                                                             >  1:1537515/1‑149 (MQ=255)
                            ccaccTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTa                                                                                                                          >  1:1568297/1‑149 (MQ=255)
                             caccTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttttttc                                                                                                                                          <  2:1126018/132‑3 (MQ=255)
                             caccTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttttttc                                                                                                                                          >  1:1126018/1‑130 (MQ=255)
                             caccTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTAc                                                                                                                         <  2:1196901/149‑1 (MQ=255)
                             aaactctGTTTTAAATTCGAGACTAGTGTAAAGCGTCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTATAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTAc                                                                                                                         <  2:348006/146‑1 (MQ=255)
                               tgccatttttAAATTCGAGAATAGTGGAAACCGGCAAACGAATGGTGTAAACATGCTGATTTTTTAGTAATTATTTCGAGAGGGATATGTTTCTAATTCTAAGAAAAAAGTTGCCGTAGCACTTTTTTAATAGAGAGTTTTTGTTACca                                                                                                                       <  2:1416141/143‑1 (MQ=255)
                               ccTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACca                                                                                                                       >  1:753012/1‑149 (MQ=255)
                                   tGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGATAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACcacaca                                                                                                                   >  2:692692/1‑149 (MQ=255)
                                    gTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcag                                                                                                                  <  2:1047322/149‑1 (MQ=255)
                                     ttttAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagc                                                                                                                 >  2:1218685/1‑149 (MQ=255)
                                           aTTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagccag                                                                                                           >  1:985041/1‑149 (MQ=255)
                                           aTTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagccag                                                                                                           >  1:979752/1‑149 (MQ=255)
                                             tCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGTGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagccagca                                                                                                         <  2:1510734/149‑1 (MQ=255)
                                               gcgACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagccagcagc                                                                                                       >  1:1048569/1‑149 (MQ=255)
                                                  aCTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTa                                                                                                    <  1:898888/149‑1 (MQ=255)
                                                     aGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGc                                                                                                 <  2:1537515/149‑1 (MQ=255)
                                                       tgtAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGcta                                                                                               >  1:1097370/1‑147 (MQ=255)
                                                       tgtAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGa                                                                                               <  2:1355938/149‑1 (MQ=255)
                                                         tAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGt                                                                                             >  2:44605/1‑149 (MQ=255)
                                                           aaGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTAGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCTTAGCACATTTTTAATAGAGAGGTTTTTTTAACACACATCAGCCAGCAGCGTATGCTAGTcc                                                                                           >  1:1009638/1‑149 (MQ=255)
                                                               ggCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAAAGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTa                                                                                       <  2:892440/149‑1 (MQ=255)
                                                                  aaaCGAATTGGGTAAACCTGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCa                                                                                    <  2:856139/149‑1 (MQ=255)
                                                                  aaaCGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCa                                                                                    <  1:1219627/149‑1 (MQ=255)
                                                                       aaTGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTCTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATaaaa                                                                               <  2:914630/149‑1 (MQ=255)
                                                                         tGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGATGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATc                                                                             <  2:715499/149‑1 (MQ=255)
                                                                            ggTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAAc                                                                          <  1:863523/149‑1 (MQ=255)
                                                                             gTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACg                                                                         >  1:490225/1‑149 (MQ=255)
                                                                             gTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACg                                                                         >  1:415478/1‑149 (MQ=255)
                                                                                  cATGCTGATTTTTTAGTATATATTTCGAGAGTGATATGTTTCTAATGCTAATAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGACTGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGAcat                                                                    <  1:1316073/149‑1 (MQ=255)
                                                                                   aTGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGAcatc                                                                   >  1:456657/1‑149 (MQ=255)
                                                                                     gCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGAcatcat                                                                 <  2:1458214/149‑1 (MQ=255)
                                                                                       ggATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACAtcatca                                                               >  1:1065358/1‑149 (MQ=255)
                                                                                             tttAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCgaga                                                         <  1:419313/149‑1 (MQ=255)
                                                                                                aGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAggg                                                      >  2:249698/1‑149 (MQ=255)
                                                                                                  tAAATATTTCGAGAGTGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCa                                                    <  2:635732/149‑1 (MQ=255)
                                                                                                  tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCa                                                    >  1:1365016/1‑149 (MQ=255)
                                                                                                  tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCa                                                    >  2:342614/1‑149 (MQ=255)
                                                                                                  tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCa                                                    >  2:1573930/1‑149 (MQ=255)
                                                                                                    aaTATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCaca                                                  >  2:609181/1‑147 (MQ=255)
                                                                                                    aaTATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGt                                                  >  2:322742/1‑149 (MQ=255)
                                                                                                      tatTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGa                                                <  2:533765/149‑1 (MQ=255)
                                                                                                       atTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGAt                                               >  1:1466865/1‑149 (MQ=255)
                                                                                                       atTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGAt                                               >  1:1472849/1‑149 (MQ=255)
                                                                                                            gagaGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCga                                          <  2:118174/149‑1 (MQ=255)
                                                                                                            gagaGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCga                                          <  1:941812/149‑1 (MQ=255)
                                                                                                             agagGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCgat                                         >  1:792258/1‑149 (MQ=255)
                                                                                                                gggATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCTGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCgatgat                                      <  2:1179397/149‑1 (MQ=255)
                                                                                                                 ggATATGTTTCTTATGCTAAGAAAAAAGGGGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                     <  1:792673/149‑1 (MQ=255)
                                                                                                                 ggATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                     >  1:1126170/1‑149 (MQ=255)
                                                                                                                 ggATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                     <  2:1568297/149‑1 (MQ=255)
                                                                                                                  gATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACAACACAGCAGCCCGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATAATCACCGAGAGGGCAGTGATGGCGATGATag                                    >  2:842566/1‑149 (MQ=255)
                                                                                                                   atatGTTTCTAATGCTAAGAAAACAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCCCACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACTGACATCATCACCGAGAGGGCAGTGATGGCGATGATaga                                   <  2:786722/149‑1 (MQ=255)
                                                                                                                    tatGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATagag                                  <  1:1280851/149‑1 (MQ=255)
                                                                                                                        tttCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGc                              <  2:615309/149‑1 (MQ=255)
                                                                                                                        tttCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGc                              <  1:2576/149‑1 (MQ=255)
                                                                                                                            tAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGaa                          <  2:753012/149‑1 (MQ=255)
                                                                                                                                 cTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCt                     >  1:969074/1‑149 (MQ=255)
                                                                                                                                   tattaaaaaGGTGCTACGGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGAAGTGATGGCGATGAAAGAGAAGCCGAACAGCTTg                   >  1:1203894/5‑149 (MQ=255)
                                                                                                                                   aaGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTg                   <  1:692828/149‑1 (MQ=255)
                                                                                                                                    attaaaaaGGTGCTACGGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgc                  >  2:966597/4‑149 (MQ=255)
                                                                                                                                    aGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgc                  <  2:857354/149‑1 (MQ=255)
                                                                                                                                     ttaaaaaGGTGCTACGGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcg                 >  1:919541/3‑149 (MQ=255)
                                                                                                                                      aaaaaaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcgc                >  2:1097603/1‑149 (MQ=255)
                                                                                                                                          aatgTGCCGTAGCACCTTTTTAATAGAGCGGGTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGGCCGGTACCATAAAAGCAACGGACAGCATCACCGAGTGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGccc            <  1:803863/146‑1 (MQ=255)
                                                                                                                                          aaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAACCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGccc            <  2:499562/149‑1 (MQ=255)
                                                                                                                                               gCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAAAGCAGCCACCAGCGTATGCGAGCCCGGTCCCATAAAATCAACCGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGAtt       <  1:1209522/149‑1 (MQ=255)
                                                                                                                                                 cGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCt     <  2:1048569/149‑1 (MQ=255)
                                                                                                                                                 cGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATGCt     <  2:713152/149‑1 (MQ=255)
                                                                                                                                                    aGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGtc  <  1:923168/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCTGTC  >  NC_000913/446641‑446937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑