Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,274,683 T→C 6.9% intergenic (+22/+77) ssb → / ← yjcB single‑stranded DNA‑binding protein/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,274,6830TC 6.9% 306.8 / 9.8 101intergenic (+22/+77)ssb/yjcBsingle‑stranded DNA‑binding protein/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (41/53);  new base C (4/3);  total (45/56)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 106 total aligned reads displayed.

TCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTG  >  NC_000913/4274535‑4274831
                                                                                                                                                    |                                                                                                                                                    
tCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGtt                                                                                                                                                      >  1:952688/1‑149 (MQ=255)
      tCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTg                                                                                                                                                >  1:607139/1‑149 (MQ=255)
        agcagcCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACTAGCCTCCTATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGtt                                                                                                                                              <  1:1074999/149‑1 (MQ=255)
        agcagcCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGtt                                                                                                                                              <  2:352144/149‑1 (MQ=255)
          cagcCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGtttt                                                                                                                                            >  1:352454/1‑149 (MQ=255)
            gcCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTaa                                                                                                                                          >  1:80113/1‑149 (MQ=255)
            gcCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTAGAGCCCGCTGCAGTCAGCTCCGTCAGCGCCGTCTAACTAGCCGCAGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATATAACCTATTGTTTTaa                                                                                                                                          <  2:314318/149‑1 (MQ=255)
             ccGCAGGGTGGCAACCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAg                                                                                                                                         <  2:43951/149‑1 (MQ=255)
              cGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAgg                                                                                                                                        <  2:51961/149‑1 (MQ=255)
               gCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGt                                                                                                                                       <  1:1484158/149‑1 (MQ=255)
               gCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGt                                                                                                                                       <  2:1597975/149‑1 (MQ=255)
                cAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTg                                                                                                                                      >  1:418390/1‑149 (MQ=255)
                 aGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTgg                                                                                                                                     <  2:942628/149‑1 (MQ=255)
                  ggggggCAATCACTTCCGCGGCGGCGCGCAGGCTCGCCCGCAGCAGTCCGCTCCGGCATCGCCGTCTAACGAGCCGCCGTTGGACTTTGATGTTGACATTCCGTTCTGCTTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGga                                                                                                                                    <  1:880563/149‑1 (MQ=255)
                    gTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACGGTGATGATGACATTCCGTTCTGAGTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGgttg                                                                                                                                  >  1:271134/1‑149 (MQ=255)
                     tGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGgatga                                                                                                                                 <  1:520158/149‑1 (MQ=255)
                         aaTCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGt                                                                                                                                       <  1:213916/139‑1 (MQ=255)
                         aaTCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTaa                                                                                                                             >  2:710536/1‑149 (MQ=255)
                          aTCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTaaa                                                                                                                            <  2:105217/149‑1 (MQ=255)
                          aTCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTGCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTaaa                                                                                                                            <  2:1199319/149‑1 (MQ=255)
                           tCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAg                                                                                                                           <  1:1025528/149‑1 (MQ=255)
                              gTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCAt                                                                                                                        >  2:708644/1‑149 (MQ=255)
                               ttCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATc                                                                                                                       >  1:908746/1‑149 (MQ=255)
                                tCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCt                                                                                                                      <  2:1276673/149‑1 (MQ=255)
                                 cAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTg                                                                                                                     >  2:227216/1‑149 (MQ=255)
                                     ggcggcgCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTgccag                                                                                                                 <  2:1174400/149‑1 (MQ=255)
                                      gcggcgCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTgccagc                                                                                                                <  1:808495/149‑1 (MQ=255)
                                       cggcgCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTgccagcc                                                                                                               >  2:674556/1‑149 (MQ=255)
                                         gcgcgcAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCAt                                                                                                             >  2:1494998/1‑149 (MQ=255)
                                            cgcAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATaaa                                                                                                          <  2:53021/149‑1 (MQ=255)
                                              cAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATaaaaa                                                                                                        <  2:124267/149‑1 (MQ=255)
                                                     gCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGcc                                                                                                 >  2:652874/1‑149 (MQ=255)
                                                       ccGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTc                                                                                               >  1:1017639/1‑149 (MQ=255)
                                                           agcagTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGtt                                                                                           <  1:1082095/149‑1 (MQ=255)
                                                             cagTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGTTGACATTCCGTTCTGATTTGTCATTAAAACAATATGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTAt                                                                                         <  1:586937/149‑1 (MQ=255)
                                                             cagTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTAt                                                                                         <  1:182267/149‑1 (MQ=255)
                                                                tCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGa                                                                                      <  1:927345/149‑1 (MQ=255)
                                                                 ccGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAg                                                                                     >  2:1318988/1‑149 (MQ=255)
                                                                  cGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCATAACGGAgg                                                                                    <  1:1112589/149‑1 (MQ=255)
                                                                     gccGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCtt                                                                                 <  2:1461553/148‑1 (MQ=255)
                                                                       cGGCAGAGCAGACTAACGATAAGACGATGGACTTTGATTATGACATTACGTTATTATTTGTCATTATAACAATAGGTTATATTGTTTTAAGGTGGAGGATTAAAGCATCTGCCAGCCATAAAAAAGAAGACTCCATAACGGAGGATTCt                                                                               <  2:1000215/149‑1 (MQ=255)
                                                                        ggCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTa                                                                              >  2:1060431/1‑149 (MQ=255)
                                                                        ggCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTa                                                                              >  2:432580/1‑149 (MQ=255)
                                                                         gCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTAc                                                                             >  1:1261795/1‑149 (MQ=255)
                                                                           ttcgcCGTTTAACGAGCCGGCGTTTGAATTTTTTGTTGACTTTCCGTTCTTTTTTTTCATTAAAACATTAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCATAACGGAGGCTTCttttt                                                                           <  2:1353701/147‑5 (MQ=255)
                                                                           aGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGt                                                                           <  2:352454/149‑1 (MQ=255)
                                                                             ctccGTCTAACGAGCCGCCGATGGACGTTGATGATGAAATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTAt                                                                         <  2:1205218/147‑1 (MQ=255)
                                                                             cgcCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTAt                                                                         <  2:899218/149‑1 (MQ=255)
                                                                               ccGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCa                                                                       <  2:1560385/149‑1 (MQ=255)
                                                                                   cTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTc                                                                   <  2:952688/149‑1 (MQ=255)
                                                                                       cGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCaaaa                                                               >  1:1432973/1‑149 (MQ=255)
                                                                                          gccgccGATGGACTTAGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCa                                                            <  1:880607/149‑1 (MQ=255)
                                                                                            cgccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCAtt                                                          <  2:1364587/149‑1 (MQ=255)
                                                                                             gccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTg                                                         >  1:499230/1‑149 (MQ=255)
                                                                                              ccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTgg                                                        <  2:572629/149‑1 (MQ=255)
                                                                                              ccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTgg                                                        >  2:128566/1‑149 (MQ=255)
                                                                                                 aTGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCa                                                     >  1:1090862/1‑149 (MQ=255)
                                                                                                     aCTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATtgt                                                 >  1:755796/1‑149 (MQ=255)
                                                                                                       tttGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTgg                                               <  1:1196081/149‑1 (MQ=255)
                                                                                                       tttGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTgg                                               >  2:1473899/1‑149 (MQ=255)
                                                                                                         tgatgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCg                                             <  1:1318988/149‑1 (MQ=255)
                                                                                                           atgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGgt                                           <  1:325457/149‑1 (MQ=255)
                                                                                                            tgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCATGTCAAAATAATTTTCCATTGTGGCGgtg                                          >  2:314433/1‑149 (MQ=255)
                                                                                                            tgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATtgtg                                                <  2:1316129/143‑1 (MQ=255)
                                                                                                            tgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATtgtg                                                >  1:1316129/1‑143 (MQ=255)
                                                                                                             gatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGgtgt                                         <  2:1017639/149‑1 (MQ=255)
                                                                                                              atgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAATAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTc                                        >  2:82292/1‑149 (MQ=255)
                                                                                                               tgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTgg                                               <  2:704938/141‑1 (MQ=255)
                                                                                                               tgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTgg                                               >  1:704938/1‑141 (MQ=255)
                                                                                                               tgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCt                                       <  1:1267687/149‑1 (MQ=255)
                                                                                                                        gTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCAtct                              >  1:408189/1‑149 (MQ=255)
                                                                                                                           cTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCtctt                           <  2:471754/149‑1 (MQ=255)
                                                                                                                                ttGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTatc                      <  1:652874/149‑1 (MQ=255)
                                                                                                                                  gTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTatcat                    >  1:1184043/1‑149 (MQ=255)
                                                                                                                                  caccttAAAACAATATAACCTATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGCTTCATCTCTTCTatcat                    >  2:1398865/5‑149 (MQ=255)
                                                                                                                                   tCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTatcatc                   <  1:710536/149‑1 (MQ=255)
                                                                                                                                   tCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTatcatc                   >  2:43559/1‑149 (MQ=255)
                                                                                                                                   accttAAAACAATATAACCTATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTatcatc                   >  2:637496/4‑149 (MQ=255)
                                                                                                                                    ccttAAAACAATATAACCTATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTAtcatca                  >  2:955204/3‑149 (MQ=255)
                                                                                                                                    ccttAAAACAATATAACCTATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTAtcatca                  >  2:961097/3‑149 (MQ=255)
                                                                                                                                     cttAAAACAATATAACCTATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCAc                 <  1:955204/148‑1 (MQ=255)
                                                                                                                                     cttAAAACAATATAACCTATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCAc                 <  1:961097/148‑1 (MQ=255)
                                                                                                                                     aTTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCAc                 <  1:128566/149‑1 (MQ=255)
                                                                                                                                      ttAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCAcc                >  1:176258/1‑149 (MQ=255)
                                                                                                                                        aaaaCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGa              >  2:1232088/1‑149 (MQ=255)
                                                                                                                                         aaaCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCtcttct                         >  2:956820/1‑137 (MQ=255)
                                                                                                                                         aaaCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCtcttct                         <  1:956820/137‑1 (MQ=255)
                                                                                                                                         aaaCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGAc             >  1:919070/1‑149 (MQ=255)
                                                                                                                                         aaaCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGAc             >  2:231713/1‑149 (MQ=255)
                                                                                                                                         aaaCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGAc             >  2:1096565/1‑149 (MQ=255)
                                                                                                                                         aaaCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCAACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGAc             >  1:96979/1‑149 (MQ=255)
                                                                                                                                          aaCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCGCTTCTATCTTCACCGAca            >  2:244679/1‑148 (MQ=255)
                                                                                                                                           aCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTc           <  2:596696/149‑1 (MQ=255)
                                                                                                                                             aaTAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGa         <  2:908746/149‑1 (MQ=255)
                                                                                                                                             aaTAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGa         >  2:5951/1‑149 (MQ=255)
                                                                                                                                              aTAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGTAGGATTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGAt        >  1:30672/1‑149 (MQ=255)
                                                                                                                                              aTAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGAt        <  1:984650/149‑1 (MQ=255)
                                                                                                                                              aTAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGAt        <  2:826031/149‑1 (MQ=255)
                                                                                                                                                   ttATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGAtgttgt   >  2:867964/1‑149 (MQ=255)
                                                                                                                                                    tataTTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGCAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTtgtg  <  2:418390/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTG  >  NC_000913/4274535‑4274831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: