Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,281,680 T→A 5.1% intergenic (+51/+103) yjcE → / ← yjcF putative cation/proton antiporter/pentapeptide repeats protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,281,6800TA 5.1% 399.9 / 7.3 128intergenic (+51/+103)yjcE/yjcFputative cation/proton antiporter/pentapeptide repeats protein
Reads supporting (aligned to +/- strand):  ref base T (66/55);  new base A (5/2);  total (71/57)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 132 total aligned reads displayed.

ACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACT  >  NC_000913/4281532‑4281828
                                                                                                                                                    |                                                                                                                                                    
accTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGCGCAATCtt                                                                                                                                                      >  1:877301/1‑149 (MQ=255)
accTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGCGCAATCtt                                                                                                                                                      >  1:1373332/1‑149 (MQ=255)
      gcgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCAc                                                                                                                                                <  2:97780/149‑1 (MQ=255)
         ccACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCTAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCtt                                                                                                                                             >  1:303739/1‑149 (MQ=255)
          cacaCGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCttt                                                                                                                                            >  1:1086598/1‑149 (MQ=255)
          cacaCGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCttt                                                                                                                                            >  1:1526986/1‑149 (MQ=255)
          cacaCGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAAAAGATTGCACCttt                                                                                                                                            <  1:135668/149‑1 (MQ=255)
               gggAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAg                                                                                                                                       >  1:742244/1‑149 (MQ=255)
               gggAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAg                                                                                                                                       >  1:998851/1‑149 (MQ=255)
                ggAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAgg                                                                                                                                      >  1:550407/1‑149 (MQ=255)
                  agaTCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGtt                                                                                                                                    >  2:1305465/1‑149 (MQ=255)
                  agaTCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGtt                                                                                                                                    <  2:1539482/149‑1 (MQ=255)
                    aTCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCCCCTTTAACAGGTTAg                                                                                                                                  >  2:1108332/1‑149 (MQ=255)
                    aTCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTATTGCACCTATAACAGGTTAg                                                                                                                                  >  2:962074/1‑149 (MQ=255)
                     tCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGc                                                                                                                                 >  1:83561/1‑149 (MQ=255)
                         cAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCgg                                                                                                                             >  2:1033461/1‑149 (MQ=255)
                           aCGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTACTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGCGCTAACCTGTTAAAGGTGCAAAAGATTGCACCTTTTTCAGATTACCTCGGGc                                                                                                                           <  1:117356/149‑1 (MQ=21)
                             gAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTa                                                                                                                         >  1:134243/1‑149 (MQ=255)
                                    tGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCa                                                                                                                  <  2:409448/149‑1 (MQ=255)
                                     gCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCAt                                                                                                                 <  2:1320829/149‑1 (MQ=255)
                                      cAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATg                                                                                                                >  2:18144/1‑149 (MQ=255)
                                       aaaaaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGa                                                                                                               >  2:411542/1‑149 (MQ=255)
                                       aaaaaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGa                                                                                                               >  1:554240/1‑149 (MQ=255)
                                          aaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTc                                                                                                            >  1:305136/1‑149 (MQ=255)
                                               cTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTaaaa                                                                                                       <  2:723328/149‑1 (MQ=255)
                                                tGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAAc                                                                                                      >  1:892275/1‑149 (MQ=255)
                                                tGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAAc                                                                                                      >  1:724548/1‑149 (MQ=255)
                                                  cACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCg                                                                                                                              >  2:302914/1‑123 (MQ=255)
                                                  cACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCg                                                                                                                              <  1:302914/123‑1 (MQ=255)
                                                  cACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTc                                                                                                            <  2:1400227/141‑1 (MQ=255)
                                                  cACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTc                                                                                                            >  1:1400227/1‑141 (MQ=255)
                                                  cACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTaccctga                                                                                                    >  1:484657/1‑143 (MQ=255)
                                                       tctcGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaataaa                                                                                               >  2:1294065/1‑149 (MQ=255)
                                                        ctcGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaataaat                                                                                              <  2:1494115/149‑1 (MQ=255)
                                                              ttGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtctc                                                                                        >  2:139885/1‑149 (MQ=255)
                                                              ttGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtctc                                                                                        >  1:1485086/1‑149 (MQ=255)
                                                                gCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtctctc                                                                                      <  2:723154/149‑1 (MQ=255)
                                                                 cTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATctctct                                                                                     <  2:1086598/149‑1 (MQ=255)
                                                                  ttGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCtt                                                                                    <  2:914614/149‑1 (MQ=255)
                                                                  ttGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCtt                                                                                    >  1:1122258/1‑149 (MQ=255)
                                                                     aatctttGCTAATTGAGGAACATCAGTAAGCCGAAAGCGACGTTACCCGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTa                                                                                 <  2:812971/144‑1 (MQ=255)
                                                                     aaGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTa                                                                                 <  1:50811/149‑1 (MQ=255)
                                                                       gCGTTTCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTaaa                                                                               <  1:1023773/149‑1 (MQ=255)
                                                                          ttGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtc                                                                                          <  2:1487675/135‑1 (MQ=255)
                                                                            gCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCaa                                                                          >  2:318578/1‑149 (MQ=255)
                                                                            gCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCaa                                                                          >  2:236152/1‑149 (MQ=255)
                                                                              tAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAAACTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAAtt                                                                        >  1:776947/1‑149 (MQ=255)
                                                                                 ttGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCTTGATTCTAAAACAAATAAATTATCTCTCTTTTAAATTCAATTCAt                                                                     >  2:820970/1‑149 (MQ=255)
                                                                                   gAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTCAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTg                                                                   <  2:11522/149‑1 (MQ=255)
                                                                                    aGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTgg                                                                  >  1:634588/1‑149 (MQ=255)
                                                                                     ggAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGc                                                                 <  2:995788/149‑1 (MQ=255)
                                                                                      gAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCt                                                                >  2:113399/1‑149 (MQ=255)
                                                                                      gAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCt                                                                <  1:591099/149‑1 (MQ=255)
                                                                                      gAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCt                                                                >  1:1077823/1‑149 (MQ=255)
                                                                                       aaaaTCAGTAAGCAGAAAGCGACTTAACCAGGCCCTAGTTAATCTGAAAAAGTTGCAATGTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTATAGTCAATTCATTGGCTg                                                               <  2:81698/149‑1 (MQ=255)
                                                                                        aaaTCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGt                                                              >  2:1467056/1‑149 (MQ=255)
                                                                                          aTCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGttc                                                            <  1:1161982/149‑1 (MQ=255)
                                                                                             aGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGttcttc                                                         <  2:83561/149‑1 (MQ=255)
                                                                                             aGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCGAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTATAAAGACAATTCATTGGCTGttattc                                                         >  1:102411/1‑149 (MQ=255)
                                                                                               tAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGa                                                       <  2:541749/149‑1 (MQ=255)
                                                                                                 aGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACt                                                     <  2:37092/149‑1 (MQ=255)
                                                                                                 aGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACt                                                     >  2:1447737/1‑149 (MQ=255)
                                                                                                   ccGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTa                                                   >  1:1254028/1‑149 (MQ=255)
                                                                                                    cGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTAATCGAATTaa                                                  >  1:1426219/1‑149 (MQ=255)
                                                                                                            aCGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATc                                          >  2:338084/1‑149 (MQ=255)
                                                                                                                aaCCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCa                                      <  1:198138/149‑1 (MQ=255)
                                                                                                                aaCCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCa                                      <  1:1143752/149‑1 (MQ=255)
                                                                                                                aaCCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCa                                      <  2:1122258/149‑1 (MQ=255)
                                                                                                                 aCCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCAt                                     <  2:550407/149‑1 (MQ=255)
                                                                                                                   cAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCt                                   >  2:664676/1‑149 (MQ=255)
                                                                                                                    aGGCCCGAGGTAATCTGAAAAATGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCtt                                  <  1:96504/149‑1 (MQ=255)
                                                                                                                       cccTAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCa                               <  2:1156107/149‑1 (MQ=255)
                                                                                                                       cccGAGGTATTCTGAAAAAGGTGCAATCTTTTTCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCa                               <  2:1067547/149‑1 (MQ=255)
                                                                                                                       cccGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCa                               <  2:1526986/149‑1 (MQ=255)
                                                                                                                        ccGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCat                              >  2:515901/1‑149 (MQ=255)
                                                                                                                        ccGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCat                              >  1:1001585/1‑149 (MQ=255)
                                                                                                                             gTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAatat                         <  2:454313/149‑1 (MQ=255)
                                                                                                                             gTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAatat                         >  1:1507392/1‑149 (MQ=255)
                                                                                                                               aaTCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCt                       >  1:667967/1‑149 (MQ=255)
                                                                                                                               aaTCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCt                       <  1:1512302/149‑1 (MQ=255)
                                                                                                                               aaTCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCt                       <  1:180417/149‑1 (MQ=255)
                                                                                                                                aTCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTg                      >  2:1205127/1‑149 (MQ=255)
                                                                                                                                 tCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGc                     >  1:1515876/1‑149 (MQ=255)
                                                                                                                                  cTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGcg                    >  2:185516/1‑148 (MQ=255)
                                                                                                                                   tgttaaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCat                   >  1:1573273/5‑148 (MQ=255)
                                                                                                                                   tgttaaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAg                   >  1:1573279/5‑149 (MQ=255)
                                                                                                                                     ttaaaGGTGCAAAAGATTGCTCCTTTAACAGGTTAGCTCGGGATAAAATTCATGATTCTAAAACAAATAAATTAACTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGAATTAACCTAAATATGCATCTTGCATAATATCTGCAGTa                 >  1:1029742/3‑149 (MQ=255)
                                                                                                                                     aaaaaGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTa                 >  1:1282045/1‑149 (MQ=255)
                                                                                                                                     aaaaaGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTa                 >  1:661602/1‑149 (MQ=255)
                                                                                                                                     aaaaaGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTa                 >  1:980918/1‑149 (MQ=255)
                                                                                                                                          ggTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCtt            <  2:90560/149‑1 (MQ=255)
                                                                                                                                              cAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATa        >  1:191248/1‑149 (MQ=255)
                                                                                                                                               aaTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAg       >  1:1592639/1‑149 (MQ=255)
                                                                                                                                               aaTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAg       >  1:474633/1‑149 (MQ=255)
                                                                                                                                               aaTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAg       >  2:46043/1‑149 (MQ=255)
                                                                                                                                               aaTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTAATAg       >  2:1131377/1‑149 (MQ=255)
                                                                                                                                                aTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGc      >  1:1282955/1‑149 (MQ=255)
                                                                                                                                                aTCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGc      >  2:463103/1‑149 (MQ=255)
                                                                                                                                                  cTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGa    >  2:1534234/1‑149 (MQ=255)
                                                                                                                                                    tttGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACt  <  2:191248/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACT  >  NC_000913/4281532‑4281828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: