Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,060 C→T 38.1% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT38.1% 43.5 / 69.8 69intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base T (19/7);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 7.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

GGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCC  >  NC_000913/4295915‑4296207
                                                                                                                                                 |                                                                                                                                                   
ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                                                                  >  1:388318/1‑149 (MQ=32)
ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                                                                  <  2:1427947/149‑1 (MQ=32)
 gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                                                                                                                                 >  2:643600/1‑149 (MQ=17)
 gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                                                                                                                                 >  2:1383626/1‑149 (MQ=17)
    atggCTTTTATGCCGCATAAGTCTTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                                                                              <  2:1567210/147‑1 (MQ=11)
    aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt                                                                                                                                              <  1:1290640/149‑1 (MQ=32)
    aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTACGCCGCATCCGACATCAATGCCTGATg                                                                                                                                             >  1:794745/1‑149 (MQ=12)
      ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                            >  2:376158/1‑149 (MQ=17)
      ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                            >  1:955544/1‑149 (MQ=32)
       gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                           >  2:928969/1‑149 (MQ=17)
         gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                                                                         >  1:748422/1‑149 (MQ=17)
               gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAAGCCTGATGCGACGCTTGc                                                                                                                                   >  1:223071/1‑149 (MQ=18)
                ccGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                                                                  >  1:1460231/1‑149 (MQ=21)
                 cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCGGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGATTAAGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcg                                                                                                                                 >  1:175835/1‑149 (MQ=255)
                     tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                             >  2:1236343/1‑149 (MQ=25)
                       cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                           <  1:318948/149‑1 (MQ=25)
                       cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                           >  2:717609/1‑149 (MQ=25)
                        gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                          >  2:437095/1‑149 (MQ=25)
                        gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                          >  2:239583/1‑149 (MQ=25)
                        gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                          >  1:1365618/1‑149 (MQ=37)
                        gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                          >  1:1101994/1‑149 (MQ=37)
                          cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCATGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCAGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                        >  2:906155/1‑149 (MQ=255)
                            tCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                      <  2:1402026/149‑1 (MQ=37)
                            tCAACGCCTGATGCGACGCATAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGAGTTTGTAGGTAGGATAAGTCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAGCAg                                                                                                                      >  2:1127503/1‑149 (MQ=11)
                             cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCGTCCGACATCAATGCCTGATGCGACGCTTGc                                                                                                                                   <  2:1336331/135‑1 (MQ=18)
                             cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCGTCCGACATCAATGCCTGATGCGACGCTTGc                                                                                                                                   >  1:1336331/1‑135 (MQ=18)
                              aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                              <  1:428680/139‑1 (MQ=37)
                              aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                              >  2:428680/1‑139 (MQ=37)
                              aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                    <  2:379808/149‑1 (MQ=37)
                               aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                   >  1:899660/1‑149 (MQ=25)
                                  cccgATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAACGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                                                                <  1:480978/146‑1 (MQ=255)
                                  ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat                                                                                                                >  2:37126/1‑148 (MQ=21)
                                   cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc                                                                                                               >  1:1016441/1‑147 (MQ=18)
                                     gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTAAGCGTGATTTTGTAGGTCGGATAAGGAGTTTACGCCGCATCCGACATCAACGACTGATGCGACGCTTGCAGCGTCTTATCAGGCCAACGcc                                                                                                             >  2:671986/1‑149 (MQ=11)
                                      aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                                                                            >  1:1006597/1‑144 (MQ=255)
                                        gCGACGCTTCACGCGTCTTATCAGGCCTACGCCAGACAGATCATTTGCCTGATTTAGCTTGATTTTGTATGTCGGATATGGCGTTTACGCCGCATCAGACTTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa                                                                                                          <  2:593296/149‑1 (MQ=11)
                                         cGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAc                                                                                                         >  1:1420387/1‑149 (MQ=37)
                                                ttaCGGGTCTTATCAGGCCGACGCCAGTCCCATCAATATCAAGATTTAGCGTGATTTTGTCGGTCGGTTATGGCTTTTACGCCGCATCCGTCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                  <  1:154596/147‑1 (MQ=11)
                                                tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                  <  2:388318/149‑1 (MQ=37)
                                                        cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc                                                                                                                                         <  2:1122713/102‑1 (MQ=32)
                                                        cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc                                                                                                                                         >  1:1122713/1‑102 (MQ=32)
                                                            tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGCCAGACAGCGCAAAAGCCTGaagta                                                                                      >  2:1175682/1‑145 (MQ=255)
                                                                  cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                         <  1:999152/108‑1 (MQ=38)
                                                                  cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                         >  2:999152/1‑108 (MQ=40)
                                                                        cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                          <  2:1420387/149‑1 (MQ=37)
                                                                         aGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                                                                         <  2:754063/149‑1 (MQ=37)
                                                                             aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGTGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                      <  1:289087/100‑1 (MQ=255)
                                                                             aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGAGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                      >  2:289087/1‑100 (MQ=255)
                                                                                 cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                              <  2:334679/88‑1 (MQ=255)
                                                                                 cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc                                                                                                               <  1:293820/103‑3 (MQ=25)
                                                                                 cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatc                                                                                                               >  2:293820/1‑101 (MQ=25)
                                                                                 cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                              >  1:334679/1‑88 (MQ=255)
                                                                                       ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAt                                                                                         >  2:366875/1‑119 (MQ=17)
                                                                                        cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                                <  2:938906/111‑1 (MQ=39)
                                                                                                 cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAcgccgc                                                 >  2:1395320/1‑149 (MQ=21)
                                                                                                 cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGc                                                 >  2:1188432/1‑149 (MQ=255)
                                                                                                              gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAttt                                                                             >  2:229667/1‑108 (MQ=40)
                                                                                                                        gCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                         <  2:1158265/54‑1 (MQ=255)
                                                                                                                        gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                         >  1:1158265/1‑54 (MQ=255)
                                                                                                                         ccgttccgccgcCCCCGACATCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                         <  1:1127503/143‑1 (MQ=11)
                                                                                                                         cGTTTACTCCGCATCAGTGTTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                         <  1:1146200/149‑1 (MQ=11)
                                                                                                                         cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGa                         <  2:1101994/149‑1 (MQ=37)
                                                                                                                          gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAc                        <  1:894159/149‑1 (MQ=37)
                                                                                                                          gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGAGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAc                        >  2:1341672/1‑149 (MQ=37)
                                                                                                                          gTTCACGCCGCATCCGACATCATCCCCTGAAGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAc                        <  1:18431/149‑1 (MQ=255)
                                                                                                                                 ccgcATCCGACATCTCCGCCTTATGCGACGCTTTCCGCGTCTTATCATGCCTACGCCATATTGCTTAATATCATTATTTAGCGTGATTTTGTATGTCGGATAAGTCTTTTATGCATCATGAGACATCAACGCCTGATGCGACGCTTAAc                 <  2:64745/149‑1 (MQ=2)
                                                                                                                                      accGACATCAACGCCTGATGCGACGCTTTCCGAGTCTTATCAGGCCTACGCCAGACAGGTCAATATCCTGATTTATCGTGATTTTGTATGTCGTATAAGTCGTTTATTCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc            <  2:175835/148‑1 (MQ=11)
                                                                                                                                                aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGcc  >  2:1233987/1‑149 (MQ=31)
                                                                                                                                                aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTTATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTAAGCCGCATCCGACATCAAAGCCTGATGCGACGCTTAACGCGTCTTATCAggac  >  2:1148822/1‑147 (MQ=11)
                                                                                                                                                 |                                                                                                                                                   
GGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCC  >  NC_000913/4295915‑4296207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: