Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,407,867 A→T 5.4% K405M (AAG→ATG)  rnr → exoribonuclease R, RNase R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,407,8670AT 5.4% 235.3 / 3.0 74K405M (AAG→ATG) rnrexoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base A (32/38);  new base T (2/2);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

GAGATGACGGTTTCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAGCGTAACGAC  >  NC_000913/4407719‑4408015
                                                                                                                                                    |                                                                                                                                                    
gagaTGACGGTTTCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTaa                                                                                                                                                      <  1:1175911/149‑1 (MQ=255)
   aTGACGGTTTCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCCCGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGCGCAGTACGCCCCGCTGGTTAAGCa                                                                                                                                                   >  2:160871/1‑149 (MQ=255)
     gACGGTTTCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCAtc                                                                                                                                                 >  2:428359/1‑149 (MQ=255)
       cGGTTTCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCAtctc                                                                                                                                               <  2:810698/149‑1 (MQ=255)
       cGGTTTCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGAGTTTATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCAtctc                                                                                                                                               <  2:16079/149‑1 (MQ=255)
          tttCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGaa                                                                                                                                            >  1:397680/1‑149 (MQ=255)
          tttCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGaa                                                                                                                                            <  1:373738/149‑1 (MQ=255)
               tcgAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCAGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAagagat                                                                                                                                       >  1:693351/1‑147 (MQ=255)
                 gAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGc                                                                                                                                     >  2:1041106/1‑149 (MQ=255)
                 gAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGc                                                                                                                                     >  1:339031/1‑149 (MQ=255)
                     ggCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATaa                                                                                                                                 <  1:509968/149‑1 (MQ=255)
                      gccgccTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAAc                                                                                                                                >  2:198205/1‑149 (MQ=255)
                         gccTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTATGCAACTCTTCGAGATGCTTAAccag                                                                                                                             <  1:1098203/149‑3 (MQ=255)
                               cGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATaaa                                                                                                                       <  1:1067681/149‑1 (MQ=255)
                                  gCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTg                                                                                                                    <  2:1590035/149‑1 (MQ=255)
                                    tACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCt                                                                                                                  <  1:65545/149‑1 (MQ=255)
                                    tACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCt                                                                                                                  <  1:589622/149‑1 (MQ=255)
                                     aCAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTg                                                                                                                 <  2:144023/149‑1 (MQ=255)
                                      cAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTgg                                                                                                                >  2:458669/1‑149 (MQ=255)
                                      cAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTgg                                                                                                                >  2:1520339/1‑149 (MQ=255)
                                      cAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTACATAACCTCTATAAAGTGCTgg                                                                                                                >  1:472210/1‑149 (MQ=255)
                                         aTTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTgggca                                                                                                             >  1:277246/1‑146 (MQ=255)
                                         aTTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATa                                                                                                             >  1:571051/1‑149 (MQ=255)
                                            cTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAg                                                                                                          <  2:397680/149‑1 (MQ=255)
                                            cTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAg                                                                                                          >  1:469183/1‑149 (MQ=255)
                                             tACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACATCTATAAAGTGCTGGATAAAGc                                                                                                         >  2:1237579/1‑149 (MQ=255)
                                               cGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGccc                                                                                                       <  1:772865/149‑1 (MQ=255)
                                                 aaGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGt                                                                                                     <  1:656802/149‑1 (MQ=255)
                                                       tgatgaGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTgaagaa                                                                                               <  2:469183/149‑1 (MQ=255)
                                                           gaGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAAcgcg                                                                                           <  1:1564220/149‑1 (MQ=255)
                                                            aGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACTCgg                                                                                          >  2:1219337/1‑149 (MQ=255)
                                                                 tcACGCGCGTCTGACCTACACCAAAGTCTGGAATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTCGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATTAAGCCCGTGAAGATCGCGGTGCGa                                                                                     >  2:98360/1‑149 (MQ=255)
                                                                  cACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGAt                                                                                    <  1:198205/149‑1 (MQ=255)
                                                                  cACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGAt                                                                                    <  2:563666/149‑1 (MQ=255)
                                                                   aCGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGAtc                                                                                   <  2:527913/149‑1 (MQ=255)
                                                                   aCGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGAtc                                                                                   >  2:920151/1‑149 (MQ=255)
                                                                     tcgcgTCTGACCTAAACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGAtctc                                                                                 <  2:1105021/148‑1 (MQ=255)
                                                                     gcgcgTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGAtctc                                                                                 >  2:435777/1‑149 (MQ=255)
                                                                     gcgcgTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGAtctc                                                                                 <  1:1369481/149‑1 (MQ=255)
                                                                       gcgTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCAt                                                                               <  2:1105298/149‑1 (MQ=255)
                                                                        ggTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCAtt                                                                              <  2:725265/148‑1 (MQ=255)
                                                                          tCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTg                                                                            >  1:476317/1‑149 (MQ=255)
                                                                          gcTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTg                                                                            <  2:1476296/148‑1 (MQ=255)
                                                                           cTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTga                                                                           <  1:1041106/149‑1 (MQ=255)
                                                                           cTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTga                                                                           <  1:1524063/149‑1 (MQ=255)
                                                                               ccTACACCAAAGTCTGGCATATTCTTCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGc                                                                       <  1:1088673/149‑1 (MQ=255)
                                                                                   cacCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCgaag                                                                   <  2:514081/149‑1 (MQ=255)
                                                                                    acCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTTAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGATCGCGGGGGGATCTCATTTGAGAGCgaaga                                                                  >  1:1018876/1‑149 (MQ=255)
                                                                                    acCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCgaaga                                                                  >  2:638278/1‑149 (MQ=255)
                                                                                      cAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGaagaag                                                                >  2:1517878/1‑149 (MQ=255)
                                                                                        aaGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCg                                                              <  1:1520339/149‑1 (MQ=255)
                                                                                             tGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGtt                                                         >  1:639076/1‑149 (MQ=255)
                                                                                               gCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCa                                                       >  1:824378/1‑149 (MQ=255)
                                                                                                 atatTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCAtt                                                     <  2:571051/149‑1 (MQ=255)
                                                                                                      cTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCaa                                                <  2:339031/149‑1 (MQ=255)
                                                                                                       tGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAAc                                               >  2:917564/1‑149 (MQ=255)
                                                                                                       tGCAGGGCGATCAGGAGCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAAc                                               <  2:1300325/149‑1 (MQ=255)
                                                                                                                aTCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGc                                      >  2:657538/1‑149 (MQ=255)
                                                                                                                aTCAGGATCCGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGc                                      >  2:699647/1‑149 (MQ=255)
                                                                                                                  cAGGATCTGCGCGCGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGGGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCTTTTTCATCGCTGAACGCCg                                    >  1:430353/1‑149 (MQ=255)
                                                                                                                  cAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCg                                    <  1:151232/149‑1 (MQ=255)
                                                                                                                     gATCTGCGCGAGCAGTACGCCCCGCTGGTTAACCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGCAAGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTACATTTTCAACGCTGAACGCCgaaa                                 >  2:284117/1‑146 (MQ=255)
                                                                                                                      aTCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTAtt                                <  1:489259/149‑1 (MQ=255)
                                                                                                                          gcgcGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAAc                            <  1:920151/149‑1 (MQ=255)
                                                                                                                                  aGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGaa                    <  1:1083408/149‑1 (MQ=255)
                                                                                                                                  aGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGaa                    >  2:451633/1‑149 (MQ=255)
                                                                                                                                     aCGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAg                 <  1:659543/149‑1 (MQ=255)
                                                                                                                                     aCGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAg                 <  1:699647/149‑1 (MQ=255)
                                                                                                                                       gCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGAc               <  1:1242868/149‑1 (MQ=255)
                                                                                                                                         cccGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGAccc             >  1:1595863/1‑149 (MQ=255)
                                                                                                                                           tagaggTTATGCAACTCTTCGAGATGCTTAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAg           >  2:1379716/5‑149 (MQ=255)
                                                                                                                                            gCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAGc          <  2:824378/149‑1 (MQ=255)
                                                                                                                                            gCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGACGTATTGAACGTATCGAACAGACCCAGc          >  1:1272224/1‑149 (MQ=255)
                                                                                                                                             gaggTTATGCAACTCTTCGAGATGCTTAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGa                                                             >  1:1363529/3‑97 (MQ=25)
                                                                                                                                             gaggTTATGCAACTCTTCGAGATGCTTAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGa                                                             <  2:1363529/95‑1 (MQ=25)
                                                                                                                                                    aGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATATCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAGCGTAacgac  >  1:313608/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGATGACGGTTTCGTCGAAAGGCCGCCTGACGGGCTACAAATTCTACGAAGCGGTGATGAGCTCTCACGCGCGTCTGACCTACACCAAAGTCTGGCATATTCTGCAGGGCGATCAGGATCTGCGCGAGCAGTACGCCCCGCTGGTTAAGCATCTCGAAGAGTTGCATAACCTCTATAAAGTGCTGGATAAAGCCCGTGAAGAACGCGGTGGGATCTCATTTGAGAGCGAAGAAGCGAAGTTCATTTTCAACGCTGAACGCCGTATTGAACGTATCGAACAGACCCAGCGTAACGAC  >  NC_000913/4407719‑4408015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑