Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,454,800 C→G 7.0% L270L (CTG→CTC fbp ← fructose‑1,6‑bisphosphatase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,454,8000CG 7.0% 313.5 / 9.4 96L270L (CTG→CTCfbpfructose‑1,6‑bisphosphatase I
Reads supporting (aligned to +/- strand):  ref base C (48/41);  new base G (2/5);  total (50/46)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGAATTT  >  NC_000913/4454657‑4454946
                                                                                                                                               |                                                                                                                                                  
atatGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGCTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGttt                                                                                                                                               >  2:646756/1‑149 (MQ=255)
 tatGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTg                                                                                                                                              <  1:239101/149‑1 (MQ=255)
 tatGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTg                                                                                                                                              <  2:1048859/149‑1 (MQ=255)
   tGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGct                                                                                                                                            <  2:916738/149‑2 (MQ=255)
   tGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCGCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCTTACAGCAAACGCAGTTTGcc                                                                                                                                            <  2:258246/149‑1 (MQ=255)
      tCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTc                                                                                                                                         >  1:835667/1‑149 (MQ=255)
       cGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGCTGtat                                                                                                                                        <  1:153188/149‑3 (MQ=255)
          tGCCGACAAAGAATGATCGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCTTCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGCTGtatgag                                                                                                                                     <  1:705149/149‑6 (MQ=255)
          tGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCggg                                                                                                                                     <  1:180601/149‑1 (MQ=255)
           gCCGACAAAGAATGAACGGCGCTGGTTCAGCGTTTCCGGGATGATATCCAGAATACTCTCTTTGCCATCGCTCTCTTTACCCCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGCTGtatgagt                                                                                                                                    <  1:1007424/149‑7 (MQ=255)
           gCCGACAAAGAATGAACGGCGCTGGTGAAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCTTCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGTATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGt                                                                                                                                    <  2:223200/149‑1 (MQ=255)
            ccGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCACTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtg                                                                                                                                   >  2:471783/1‑149 (MQ=255)
              gACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggc                                                                                                                                 >  2:580296/1‑149 (MQ=255)
               aCAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggct                                                                                                                                >  1:1588977/1‑149 (MQ=255)
               aCAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggct                                                                                                                                >  2:1003104/1‑149 (MQ=255)
                 aaaGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATGTCCAGAATACGCTCTTTGCCCTCGCTCGCTTTACCGCCCGCTTGATCCGCCAGGAATGCCATCGGGATGCACTCATACAGCAAACGGAGTTTGCCGTCCGGGtggctgc                                                                                                                              >  1:1070170/1‑148 (MQ=255)
                 aaaGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctgg                                                                                                                              >  2:552404/1‑149 (MQ=255)
                 aaaGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctgg                                                                                                                              >  2:648749/1‑149 (MQ=255)
                 aaaGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGtggctgg                                                                                                                              <  1:928141/149‑1 (MQ=255)
                   ataaTGACCGGCGCTGGTGCAGGGTGTCCGGGATGATATCAAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCg                                                                                                                            <  1:1132508/147‑1 (MQ=255)
                    gAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATCCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCgg                                                                                                                           >  2:730395/1‑149 (MQ=255)
                    gAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCgg                                                                                                                           >  2:705830/1‑149 (MQ=255)
                    gAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCgg                                                                                                                           <  1:602688/149‑1 (MQ=255)
                       tGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGc                                                                                                                        >  1:788363/1‑149 (MQ=255)
                         aaCGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCtt                                                                                                                      >  2:204386/1‑149 (MQ=255)
                           cGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTgg                                                                                                                    >  1:990190/1‑149 (MQ=255)
                             gcgcTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCCTCGCTCGCATTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCCCTCATACAGCAAACGCAGTTAGCAGTCCGGGTGTCTGGCGGTGCGTgggg                                                                                                                  >  1:1474342/1‑148 (MQ=255)
                              cgcTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTa                                                                                                                 >  1:1049643/1‑149 (MQ=255)
                                 tGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTagag                                                                                                              <  1:884497/149‑1 (MQ=255)
                                 tGGGGCAGGGGTTCCTGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGGTTGTTCCGCCAGGAATGCCATCGGGTTGCCCTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTagag                                                                                                              <  2:772655/149‑1 (MQ=255)
                                   gTGCAGGGTTTCCGGGATGCTATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGAt                                                                                                            <  2:692093/149‑1 (MQ=255)
                                   gTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCTCTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGAt                                                                                                            <  1:12229/149‑1 (MQ=255)
                                     gCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGCTGtat                                                                                                                                        >  2:362679/1‑117 (MQ=255)
                                     gCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACTGCGTTTGCTGtat                                                                                                                                        <  1:362679/119‑3 (MQ=255)
                                     gCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATaa                                                                                                          >  2:1315961/1‑149 (MQ=255)
                                      cAGGGTTTCCGGGATGATATCCAGAATCCGCTCATTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACCGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATaaa                                                                                                         <  2:130588/149‑1 (MQ=255)
                                         ggTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATAc                                                                                                      >  1:318581/1‑149 (MQ=255)
                                                 ggATTATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGGTCCGCCAGGAATGCCATCGGGTTGCACTAATAGAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTc                                                                                              <  2:95312/149‑1 (MQ=255)
                                                 ggATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTc                                                                                              <  2:835667/149‑1 (MQ=255)
                                                   atgatATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTcag                                                                                            <  2:1411802/149‑1 (MQ=255)
                                                     gatATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTcagca                                                                                          >  2:1256119/1‑149 (MQ=255)
                                                         tCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGtt                                                                                      >  1:598257/1‑149 (MQ=255)
                                                           cAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTAc                                                                                    >  2:1018460/1‑149 (MQ=255)
                                                            aGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACg                                                                                   <  1:648749/149‑1 (MQ=255)
                                                             gAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACgg                                                                                  <  2:788363/149‑1 (MQ=255)
                                                              aaTACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGt                                                                                 <  1:1018460/149‑1 (MQ=255)
                                                               aTACGCTCTTTGCCATCGCGCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTg                                                                                <  1:1079424/149‑1 (MQ=255)
                                                                 aCGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGa                                                                              >  2:1104881/1‑149 (MQ=255)
                                                                   gCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGaaa                                                                            >  1:1504591/1‑149 (MQ=255)
                                                                   gCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGaaa                                                                            >  2:213514/1‑149 (MQ=255)
                                                                      ctTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATcc                                                                         >  1:436938/1‑149 (MQ=255)
                                                                          gCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGa                                                                     <  2:819256/149‑1 (MQ=255)
                                                                          gCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGa                                                                     <  1:580996/149‑1 (MQ=255)
                                                                          gCCATCGCTCGCTTTACCACCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGa                                                                     <  2:1495/149‑1 (MQ=255)
                                                                             aTCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCa                                                                  >  1:568394/1‑149 (MQ=255)
                                                                                 ctcgctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACTGTGGAAATCCGCGACCAGTGa                                                              >  2:1075639/1‑149 (MQ=255)
                                                                                 ctcgctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGa                                                              >  2:730076/1‑149 (MQ=255)
                                                                                 ctcgctTTACCGCCCGCTTGTTCCACCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATTAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGa                                                              >  2:1558889/1‑149 (MQ=255)
                                                                                   cgctTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAAc                                                            <  2:598257/149‑1 (MQ=255)
                                                                                       ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGat                                                        >  1:449030/1‑149 (MQ=255)
                                                                                       ttACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGat                                                        <  1:576185/149‑1 (MQ=255)
                                                                                           cgcccgcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATaa                                                    <  1:608744/149‑1 (MQ=255)
                                                                                           cgcccgcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATaa                                                    <  2:13986/149‑1 (MQ=255)
                                                                                                gcTTGTTCCGCCAGGAATGCCTTCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCTGTGAACCGATATAACGTGa                                               <  1:1510595/149‑1 (MQ=255)
                                                                                                gcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGa                                               <  1:493229/149‑1 (MQ=255)
                                                                                                gcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGa                                               >  1:739313/1‑149 (MQ=255)
                                                                                                gcTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGa                                               <  1:204386/149‑1 (MQ=255)
                                                                                                 cTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAg                                              <  1:1003104/149‑1 (MQ=255)
                                                                                                       ccGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATaa                                        <  2:1588977/149‑1 (MQ=255)
                                                                                                         gCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAgg                                      >  2:1285781/1‑149 (MQ=255)
                                                                                                         gCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAgg                                      >  2:876316/1‑149 (MQ=255)
                                                                                                          ccAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAggg                                     <  2:318581/149‑1 (MQ=255)
                                                                                                          ccAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAggg                                     >  1:189453/1‑149 (MQ=255)
                                                                                                            aGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCg                                   <  1:402079/149‑1 (MQ=255)
                                                                                                              gAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATCACGTGAGGTATAAGGGCGGt                                 >  1:1401555/1‑149 (MQ=255)
                                                                                                              gAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGt                                 >  2:173183/1‑149 (MQ=255)
                                                                                                                   ccATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTtggtg                            >  2:1233139/1‑149 (MQ=255)
                                                                                                                   ccATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGGCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTtggtg                            <  1:552404/149‑1 (MQ=255)
                                                                                                                     aTCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGa                          <  1:730395/149‑1 (MQ=255)
                                                                                                                     aTCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGa                          <  1:705830/149‑1 (MQ=255)
                                                                                                                        gggTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGAttt                       >  2:1296464/1‑149 (MQ=255)
                                                                                                                          gTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGGGGCTGGCGGTGCGTGGGTAGAGATAAATCCCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGGGAGGTATAAGGGCGGTTGGTGGATTTAt                     <  2:1215049/149‑1 (MQ=255)
                                                                                                                           gtGCACTCATACAGCAAACGCAGTTTGCCGGCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATCCCGCCTTTCAGCAGGTTACTGAGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTAtc                    <  2:780045/148‑1 (MQ=255)
                                                                                                                             gCACTCATACAGCAAACCCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTAtctt                  <  2:594437/149‑1 (MQ=255)
                                                                                                                               aCTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTAtcttct                >  2:345738/1‑149 (MQ=255)
                                                                                                                                 tCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCttcttc              >  1:9966/1‑149 (MQ=255)
                                                                                                                                 tCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCttcttc              >  1:525453/1‑149 (MQ=255)
                                                                                                                                   aTACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCAGCGGCCAGTGATCCGATATAACGTGAGGTATAAGGGGGGTTGGTGGATTTATCTTCTTCCt            >  1:86571/1‑149 (MQ=255)
                                                                                                                                    tACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTGCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTg           <  1:1315961/149‑1 (MQ=255)
                                                                                                                                      cAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGATTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGc         >  1:800061/1‑149 (MQ=255)
                                                                                                                                        gCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAg       >  1:827627/1‑149 (MQ=255)
                                                                                                                                          aaaCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGaa     >  1:1356186/1‑149 (MQ=255)
                                                                                                                                           aaCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGAAGAAt    >  2:152612/1‑149 (MQ=255)
                                                                                                                                            aCGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGAAtt   >  1:1079596/1‑149 (MQ=255)
                                                                                                                                             cGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGAAttt  >  1:882631/1‑149 (MQ=255)
                                                                                                                                             cGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGAAttt  >  2:276192/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
ATATGGTCGTTGCCGACAAAGAATGAACGGCGCTGGTGCAGGGTTTCCGGGATGATATCCAGAATACGCTCTTTGCCATCGCTCGCTTTACCGCCCGCTTGTTCCGCCAGGAATGCCATCGGGTTGCACTCATACAGCAAACGCAGTTTGCCGTCCGGGTGGCTGGCGGTGCTTGGGTAGAGATAAATACCGCCTTTCAGCAGGTTACGGTGGAAATCCGCGACCAGTGAACCGATATAACGTGAGGTATAAGGGCGGTTGGTGGATTTATCTTCTTCCTGGCAGAATTT  >  NC_000913/4454657‑4454946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: