Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,472,434 G→A 6.8% R34* (CGA→TGA)  pyrL ← pyrBI operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,472,4340GA 6.8% 402.6 / 11.3 131R34* (CGA→TGA) pyrLpyrBI operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (68/53);  new base A (5/4);  total (74/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGC  >  NC_000913/4472289‑4472582
                                                                                                                                                 |                                                                                                                                                    
ttcgtgTTTGGTTTCAGTTTCTCCGGTGTCGCCAGCACCAGTTTAAGGTGATCGCGACTAAGGTCGTTTATGGAAATTATATGTTTCTGATATTGCGGATTAGCCATCTTTTATCTCGTGACGCCTGGGCAAAAAAAAGCCCCTCGAtt                                                                                                                                                   <  1:1544685/144‑1 (MQ=255)
tGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGAtt                                                                                                                                                   >  1:1454751/1‑149 (MQ=255)
   gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAg                                                                                                                                                <  2:1290941/149‑1 (MQ=255)
   gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAg                                                                                                                                                <  2:644861/149‑1 (MQ=255)
   gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAg                                                                                                                                                <  1:1432646/149‑1 (MQ=255)
   gggTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAg                                                                                                                                                <  2:1464607/149‑1 (MQ=255)
           tttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCtttt                                                                                                                                        >  2:643759/1‑146 (MQ=255)
            ttCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGa                                                                                                                                       >  1:10592/1‑149 (MQ=255)
              cAGTTTCGCCGCTGGCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAAt                                                                                                                                     <  1:1001295/149‑1 (MQ=255)
               aGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCtttttttt                                                                                                                                    <  1:643759/149‑8 (MQ=255)
               aGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCCGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATg                                                                                                                                    <  2:379281/149‑1 (MQ=255)
                gTTTCGCCGCTGGCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGATTATGGAAAAGATATGTATCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgg                                                                                                                                   <  2:875133/149‑1 (MQ=255)
                  ttCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgggg                                                                                                                                 >  1:347947/1‑148 (MQ=255)
                   tCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTg                                                                                                                                <  2:362033/149‑1 (MQ=255)
                   tCGCCGCTGTCGCCAGCACAAGATTAAGGTCATCGCGACTAAGGGCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTAATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTg                                                                                                                                <  2:367562/149‑1 (MQ=255)
                     gccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAt                                                                                                                              >  2:1054092/1‑149 (MQ=255)
                      ccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATc                                                                                                                             >  2:1549121/1‑149 (MQ=255)
                           gTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACgg                                                                                                                        <  2:682284/149‑1 (MQ=255)
                            tCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACggg                                                                                                                       >  2:614562/1‑149 (MQ=255)
                               ccAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAGCAACGGgaag                                                                                                                    >  2:295879/1‑149 (MQ=255)
                                  gCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaa                                                                                                                 <  1:783914/149‑1 (MQ=255)
                                    aCCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaaa                                                                                                               >  1:16917/1‑149 (MQ=255)
                                    aCCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGaaaaa                                                                                                               >  2:496343/1‑149 (MQ=255)
                                     ccAGATTAAGGTCATCGCGACTAAGGTCGTTAATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAAc                                                                                                              <  1:1514157/149‑1 (MQ=255)
                                       aGATTAAGGGCATCGCGACTATGGGCGTTTTTGTAATTTATATGTTTCTTATATAGCGTATTATCCATGTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACgg                                                                                                            <  1:169854/149‑1 (MQ=255)
                                              ggTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCa                                                                                                     >  2:805049/1‑149 (MQ=255)
                                              ggTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCa                                                                                                     >  1:772615/1‑149 (MQ=255)
                                              ggTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGAGATCAACGGGAAGAAAAACGGCAGGccc                                                                                                     >  2:1522321/1‑148 (MQ=255)
                                               gTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGTAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAg                                                                                                    >  1:1160180/1‑149 (MQ=255)
                                                  aTCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCCGGCCAGCGt                                                                                                 >  2:1438168/1‑149 (MQ=255)
                                                     gcgACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTCGGAATCGGTGATCAACGGGAAGAAAAACGGCAGTCCAGCGTCtt                                                                                              >  2:42627/1‑149 (MQ=255)
                                                      ctaCTATGGTCGTTTATGGAAATGATATGTTTATGATATAGCGGATTAGCCTTCTTTAATCTCCTGACGCCGGGGCAAAAAAAAGCACCTCGATTGCGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCttt                                                                                             <  1:1474903/147‑1 (MQ=255)
                                                       gACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCtttt                                                                                            <  2:772615/149‑1 (MQ=255)
                                                         atAAGGTCGTTTATGGAAATGATATGTTTCTGAAATAGCGCATTAGCCTTCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCtttttt                                                                                          <  1:598963/148‑1 (MQ=255)
                                                          tAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGTTTATCCTTTTTTTATCTCCTGCCGCCTGGGCAAAAAAATGCCCCTCGATTTTGGGGCTGGGTTTGGGTGATCAACGGGATGAAATACGGCAGGCCAGCGTCttttttg                                                                                         <  1:334986/149‑2 (MQ=255)
                                                             ggTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGa                                                                                      >  2:511066/1‑149 (MQ=255)
                                                              gTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGAc                                                                                     >  1:673942/1‑149 (MQ=255)
                                                                        taaaTGATATGTTTCTGATATAGCGGTTTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGTGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGAc                                                                           <  2:687227/148‑1 (MQ=255)
                                                                        gAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGAc                                                                           >  2:1330208/1‑149 (MQ=255)
                                                                        gAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGAc                                                                           >  1:705693/1‑149 (MQ=255)
                                                                         aaaTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACa                                                                          >  2:1408982/1‑149 (MQ=255)
                                                                            tGATATGTTTCTTATATAGCTTATTAGCCATCTTTTATCTCCAGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGTGTTATCAACGTTAAGAAAAAAGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaaaa                                                                       <  1:1154993/149‑1 (MQ=255)
                                                                             gATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAAt                                                                      >  1:543222/1‑149 (MQ=255)
                                                                              atatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATAGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCttt                                                                                             <  2:304698/125‑1 (MQ=255)
                                                                              atatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATAGGTGATAAACGGGAAGAAAAACGGCAGGCCAGCGTCttt                                                                                             >  1:304698/1‑125 (MQ=255)
                                                                              atatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCGCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATg                                                                     >  2:1012136/1‑149 (MQ=255)
                                                                               tatGTTTCTGATCTAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGt                                                                    <  2:90018/149‑1 (MQ=255)
                                                                               tatGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAGCAACGGGAAGAAAAACGGCACGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGt                                                                    <  1:295879/149‑1 (MQ=255)
                                                                               tatGTTTCTGATATAGCGGATTAGCCATCTTCTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCAAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGt                                                                    >  2:1504028/1‑149 (MQ=255)
                                                                                 tGTTTCTGATATCGCGGATTAGCCATCTTTTATCTCCTGCCGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAGCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg                                                                  <  1:809643/149‑1 (MQ=255)
                                                                                    ttCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAac                                                               <  1:289874/149‑1 (MQ=255)
                                                                                     tCTGATATAGCGGATTAGCCATCTTTTTTCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca                                                              <  1:38362/149‑1 (MQ=255)
                                                                                      cTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacac                                                             <  2:1257167/149‑1 (MQ=255)
                                                                                       tGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca                                                            <  2:1023864/149‑1 (MQ=255)
                                                                                       tGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacaca                                                            <  2:302543/149‑1 (MQ=255)
                                                                                        tatatAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacacac                                                           <  2:1363819/148‑1 (MQ=255)
                                                                                        gATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacacac                                                           <  2:729673/149‑1 (MQ=255)
                                                                                           aaaGCGGATTAGCCATATTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCTATAGAGGGGCAGGGAATGTGTGATAAACGGGAAGAAAAAACGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGa                                                        >  2:1230818/3‑149 (MQ=255)
                                                                                            taGCGGATTAGCCATCTTTTATCTCCTGGCGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAGGACAAAATGTCGAACACACTGaa                                                       >  2:830732/1‑149 (MQ=255)
                                                                                            taGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGaa                                                       <  2:729242/149‑1 (MQ=255)
                                                                                            taGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGaa                                                       >  1:279794/1‑149 (MQ=255)
                                                                                             aGCTGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGTGGCTTGGAATGGGTGATCAACGGGAAGAAAAACTGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAAc                                                      <  1:1128501/149‑1 (MQ=255)
                                                                                             aGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAAc                                                      >  1:16060/1‑149 (MQ=255)
                                                                                               cGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCa                                                    >  1:1553224/1‑149 (MQ=255)
                                                                                                ggATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCAt                                                   <  2:792782/149‑1 (MQ=255)
                                                                                                     aGCCATCTTTTATCTCCTGACGCCAGGGCAAAAAAAAGCCCCTCGATTGACGGGATCGGAATGGGTGATCAACGGGAAGAAAAACCGCAGGCCAGCGTCTTTTTTCAGCCGCGGTAAGACAAAATGTCTAACACCCTGAAACATACAtc                                              >  2:1402220/1‑149 (MQ=255)
                                                                                                       ccATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGAGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGAGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAAACCCCTGAACCATACAtcct                                            >  1:985931/1‑149 (MQ=255)
                                                                                                            ttttATCTCCTGACGCCTGTGCAAAAAAAAGCCCCTAGATTGAGGGGCTGGGAATGGGTGATCAACTGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACCCTGAACCATACATACTCCCgg                                       >  1:1577056/1‑149 (MQ=255)
                                                                                                            ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCCGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGATCCATACATCCTCCCgg                                       >  1:834217/1‑149 (MQ=255)
                                                                                                            ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCgg                                       <  2:279794/149‑1 (MQ=255)
                                                                                                            ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCgg                                       >  1:54970/1‑149 (MQ=255)
                                                                                                            ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATACTCACgg                                       >  1:1327651/1‑149 (MQ=255)
                                                                                                                aTCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAt                                               <  1:624677/137‑1 (MQ=255)
                                                                                                                aTCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCaaa                                   <  1:1054092/149‑1 (MQ=255)
                                                                                                                 tctcCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAAt                                  >  1:1082779/1‑149 (MQ=255)
                                                                                                                     cTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTGGAACACACTGCACCATACATCCTCCCGGCAAATTGTc                              >  1:116246/1‑149 (MQ=255)
                                                                                                                     cTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTc                              <  2:10592/149‑1 (MQ=255)
                                                                                                                      tGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAAc                                                      >  1:744965/1‑124 (MQ=255)
                                                                                                                          gCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACTGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATTCATCCTCCCGGCAAATTGTCCGGCa                         <  1:258212/149‑1 (MQ=255)
                                                                                                                          gCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCa                         >  2:1483648/1‑149 (MQ=255)
                                                                                                                           ccTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCAt                        <  2:705693/149‑1 (MQ=255)
                                                                                                                             tGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATta                      <  2:403657/149‑1 (MQ=255)
                                                                                                                               ggCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATtata                    >  1:823635/1‑149 (MQ=255)
                                                                                                                                  aaaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGACCCATACATCCTCCCGGCAAATTGTCCGGCATTATACTc                 >  2:1199454/1‑149 (MQ=255)
                                                                                                                                  aaaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATAGATCCTCCCGGCAAATTGTCCGGCATTATACtg                 >  1:1048995/1‑148 (MQ=255)
                                                                                                                                  aaaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTc                 >  2:191250/1‑149 (MQ=255)
                                                                                                                                  aaaaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTACCGGCAAATTGTCCGGCATTATACta                 >  1:1439188/1‑148 (MQ=255)
                                                                                                                                    aaaaaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCAt               >  2:426153/1‑149 (MQ=255)
                                                                                                                                       aaaGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGt            <  1:691014/149‑1 (MQ=255)
                                                                                                                                        caGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTc           >  2:201161/2‑149 (MQ=255)
                                                                                                                                         aGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCa          >  2:1577727/1‑149 (MQ=255)
                                                                                                                                         aGCCCCTCAATCGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCa          >  2:664596/1‑149 (MQ=255)
                                                                                                                                         aGCCCCTCAATCGAGGGGCTGGGAATGCGTGATCAACGGGAAGAAAAACGGAAGGCCAGCGTCTATTTTCAGACGCGGTAAGACAAAATGACGAACACACTGAACCATACATCCTCCCCGCAAATTGTCCGGCATTAAACTCATCGTCa          >  2:1035880/1‑149 (MQ=255)
                                                                                                                                            cccTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGaa       >  1:582431/1‑149 (MQ=255)
                                                                                                                                              cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCTGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc     >  1:256469/1‑149 (MQ=255)
                                                                                                                                              cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc     >  2:645822/1‑149 (MQ=255)
                                                                                                                                              cTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc     >  1:1575587/1‑149 (MQ=255)
                                                                                                                                               tCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg    <  1:1353591/149‑1 (MQ=255)
                                                                                                                                               tCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg    <  1:1356206/149‑1 (MQ=255)
                                                                                                                                                 gATTGAGGGGCTGGTAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGACCACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcggc  <  1:1522321/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TGCGGGTTTGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGATCAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGC  >  NC_000913/4472289‑4472582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: