Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,629,646 G→A 7.8% R293C (CGC→TGC)  ettA ← energy‑dependent translational throttle A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,629,6460GA 7.8% 307.0 / 10.3 104R293C (CGC→TGC) ettAenergy‑dependent translational throttle A
Reads supporting (aligned to +/- strand):  ref base G (48/47);  new base A (2/6);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 112 total aligned reads displayed.

AGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAG  >  NC_000913/4629498‑4629794
                                                                                                                                                    |                                                                                                                                                    
aGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAgcg                                                                                                                                                      >  1:1205759/1‑149 (MQ=255)
 ggTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAgcgc                                                                                                                                                     <  1:392360/149‑1 (MQ=255)
  gTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCAGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAgcgcg                                                                                                                                                    >  1:275574/1‑149 (MQ=255)
    catcaATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAgcgcgac                                                                                                                                                  >  1:1548444/1‑147 (MQ=255)
    catcaATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGcc                                                                                                                                                  >  2:19975/1‑149 (MQ=255)
     atcaatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCATAGCGCGCCa                                                                                                                                                 >  1:131596/1‑149 (MQ=255)
      tcaatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAg                                                                                                                                                >  2:668776/1‑149 (MQ=255)
        aatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCCGCCCTTTATCGCCCAGACGCGGTCGAGGTGGAATACGCAGTTCGTTGGTTGCGTGACGTTTCTGATATTCGGTGCTGTTCAGTTCTTACATGCGCCCCacac                                                                                                                                              >  2:105503/1‑149 (MQ=255)
        aatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGAATTCAAGCACTTTATCGCCCAGACGCGTTACAGGTGGAATAAACAGTTCGATGGTTTCGTTACCTTTCTGATATTCGGTGCGGTTCAGTTCTTCAAAGCGCGACAGAc                                                                                                                                              >  2:105508/1‑149 (MQ=255)
            cgcagACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGc                                                                                                                                          <  1:1437242/148‑1 (MQ=255)
               agACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCttt                                                                                                                                       >  2:1099084/1‑149 (MQ=255)
                 aCGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTAc                                                                                                                                     <  2:166352/149‑1 (MQ=255)
                 aCGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttga                                                                                                                                     <  2:897263/149‑3 (MQ=255)
                  cGATCGCCATAGGCAGTCCGCACGTTGCCGACCTCCAGCACTTTATCGCCCACCCGCGGACCAGTAGGAATAAACAGTTCGTTGGTTTCGCGACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                    <  1:1079360/149‑4 (MQ=255)
                  cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACTCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                    <  2:797126/149‑4 (MQ=255)
                  cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTAcc                                                                                                                                    <  1:368329/149‑1 (MQ=255)
                  cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                    <  2:375411/149‑4 (MQ=255)
                    ctCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCtt                                                                                                                                  <  2:1288849/148‑1 (MQ=255)
                    aTCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCACCAGACGTGCTTTACCtt                                                                                                                                  >  1:1208071/1‑149 (MQ=255)
                     tCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaagaa                                                                                                                                 <  1:1565960/149‑7 (MQ=255)
                        ccATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGa                                                                                                                              <  2:1296346/149‑1 (MQ=255)
                         cATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGAc                                                                                                                             >  1:627/1‑149 (MQ=255)
                          aTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACt                                                                                                                            >  2:1515751/1‑149 (MQ=255)
                           tAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCttt                                                                                                                                       >  1:1309776/1‑137 (MQ=255)
                           tAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCttt                                                                                                                                       <  2:1309776/137‑1 (MQ=255)
                                   aCGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCtt                                                                                                                   <  2:705271/149‑1 (MQ=255)
                                     gCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGGTCGATGGTTTCGTGACGTGACTGAGATTCGGGGCTGTTCAGTTCTTCACAGCGCGCCAGACGTGCTTTACCTTTAGACGGACGGCCtttt                                                                                                                 >  1:219830/1‑148 (MQ=255)
                                      cAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAg                                                                                                                <  2:563046/149‑1 (MQ=255)
                                      cAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAg                                                                                                                >  2:639359/1‑149 (MQ=255)
                                       aGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGt                                                                                                               <  1:1552472/149‑1 (MQ=255)
                                         gTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTAc                                                                                                             >  2:317597/1‑149 (MQ=255)
                                            gCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                          <  2:1076862/149‑1 (MQ=255)
                                              tGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTgc                                                                                                        >  2:1338187/1‑148 (MQ=255)
                                               gACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGAc                                                                                                       <  1:668776/149‑1 (MQ=255)
                                                 cTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGt                                                                                                     <  2:1370934/149‑1 (MQ=255)
                                                       gCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                          >  1:792080/1‑138 (MQ=255)
                                                         aCTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                             <  1:1108549/149‑1 (MQ=255)
                                                              aTCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGct                                                                                        >  2:113335/1‑149 (MQ=255)
                                                               tCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGctc                                                                                       >  1:1226286/1‑149 (MQ=255)
                                                                  cccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCTTTTAGTACCTTGACGTACCCATTCCAGCTCttt                                                                                    >  1:245587/1‑149 (MQ=255)
                                                                  cccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCttt                                                                                    >  2:1580657/1‑149 (MQ=255)
                                                                    cAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGACTTTAGTACATTGACGTACCCATTCCAGCTCTTtct                                                                                  >  1:794760/1‑149 (MQ=255)
                                                                       aCGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCACCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCaa                                                                               <  2:1208071/149‑1 (MQ=255)
                                                                        cgcgGTCGAGGTGGAATAAACAGTTCGTTGGTTGCGTTACGTTTCTGATATTAGGTGCTGTTAAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGTCCTTTAGTACCTTGACGTACACATTCCAGCTCTTTCTCAAt                                                                              <  2:519067/149‑1 (MQ=255)
                                                                        cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACTTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACATTGACGTACCCATTCCAGCTCTTTCTAAAt                                                                              >  1:1018059/1‑149 (MQ=255)
                                                                        cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAAt                                                                              >  1:869586/1‑149 (MQ=255)
                                                                         gcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATc                                                                             >  1:912419/1‑149 (MQ=255)
                                                                         gcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATc                                                                             <  1:460277/149‑1 (MQ=255)
                                                                            gTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGAGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGAc                                                                          >  2:286710/1‑149 (MQ=255)
                                                                             tCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACt                                                                         <  2:1050955/149‑1 (MQ=255)
                                                                               cAGGTGGAAGACAGAGTTCGTTGGGTGCGTTACGTTTCTGTTATTCGGTGTTGTTCAGTTCTTCAAAGCGCGCCCCACGTTCTTTACCTTTCGCCTGACGGCCTTTCGTACCTTGACGTACCCATTCCAGCGCTTGCTCACTCGACTTa                                                                       <  2:1057784/149‑1 (MQ=255)
                                                                                aGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTac                                                                      >  2:176825/1‑149 (MQ=255)
                                                                                      aaTAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGAcgcg                                                                >  1:1106058/1‑149 (MQ=255)
                                                                                       aTAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACgcgc                                                               >  1:1573517/1‑149 (MQ=255)
                                                                                          aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTTCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTTCCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                            >  2:475758/1‑149 (MQ=255)
                                                                                          aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                            <  1:839508/149‑1 (MQ=255)
                                                                                          aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                            <  2:340884/149‑1 (MQ=255)
                                                                                           aCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGct                                                           >  1:476677/1‑149 (MQ=255)
                                                                                               ttCGGTGGTTTCGTTACGTTTGTGATATTCGGTGCTGATCAGTTCTTCAAAGCCCGCCAGCCGTGCGTTACCTTTCCACTGACGGCCTTTTGTACCTTGCCGTACCCAATCCATCTGTTTGTTATTCGACTTACGACGCGCCGcttctt                                                       <  1:1395133/149‑1 (MQ=255)
                                                                                                tCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTtg                                                      <  1:770598/149‑1 (MQ=255)
                                                                                                  gTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTtgtg                                                    <  2:912419/149‑1 (MQ=255)
                                                                                                    tGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGaa                                                  <  2:476677/149‑1 (MQ=255)
                                                                                                    tGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGaa                                                  <  1:176825/149‑1 (MQ=255)
                                                                                                         tCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTc                                             <  2:809432/149‑1 (MQ=255)
                                                                                                           gTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCt                                           >  1:635916/1‑149 (MQ=255)
                                                                                                              aCGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                             <  2:900924/96‑1 (MQ=255)
                                                                                                              aCGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                             >  1:900924/1‑96 (MQ=255)
                                                                                                               cGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                       <  1:1580657/149‑1 (MQ=255)
                                                                                                                gTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                      >  1:559692/1‑149 (MQ=255)
                                                                                                                gTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                      <  1:113335/149‑1 (MQ=255)
                                                                                                                gTTGCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                      <  2:275574/149‑1 (MQ=255)
                                                                                                                   tCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAgg                                   >  2:146152/1‑149 (MQ=255)
                                                                                                                     tGATATTCGGTGCTGTTCAGTTCTTCAAATCGCGCCAGACGTGCTTTACCGTTCGACTGACGGCCTTTAGTAACTTGAAGTACCCATTCCAGCTATGTCTAAAACGACTTACGACGCGACGCTTCTTGTGAAGCTTCCTCCGCCATgcg                                 >  2:1388342/1‑149 (MQ=255)
                                                                                                                     tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCCGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGAGAAGCTTCCTGCTCCCGgcg                                 >  2:1045115/1‑149 (MQ=255)
                                                                                                                     tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgcg                                 >  1:143607/1‑149 (MQ=255)
                                                                                                                      gATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTATACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCTGCTCTTTCTCAATCGACTTACGACGCGCCTCTTCTTGTGAAGCTTCCTGCGCCAGgcgc                                <  2:784408/149‑1 (MQ=255)
                                                                                                                       atatTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                               <  1:485394/149‑1 (MQ=255)
                                                                                                                       atatTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                               >  1:399475/1‑149 (MQ=255)
                                                                                                                            cGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCt                          <  2:547632/149‑1 (MQ=255)
                                                                                                                              gTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCttt                        <  1:639359/149‑1 (MQ=255)
                                                                                                                               tGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTAGACTGACGGCCTTTAGTACCTTTACTTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                       <  2:1061073/149‑1 (MQ=255)
                                                                                                                               ggCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGGGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                       <  1:322575/148‑1 (MQ=255)
                                                                                                                               ggCTGTTCAGTGCTTCACAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                       <  2:836879/148‑1 (MQ=255)
                                                                                                                                gCTGTTCAGTTCTTCATAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCTTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCt                      <  2:245587/149‑1 (MQ=255)
                                                                                                                                 cTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTg                     <  2:581547/149‑1 (MQ=255)
                                                                                                                                   gTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCt                   <  2:1573517/149‑1 (MQ=255)
                                                                                                                                       aGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccag               >  2:1298237/1‑149 (MQ=255)
                                                                                                                                        gaaaggtaaaGCACGTCTGGCGCGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc              >  2:1137341/8‑149 (MQ=255)
                                                                                                                                        gTTCTTCTAAGCGAGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTAGCTTGACGGACCCATTCGAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTAGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc              <  1:105508/149‑1 (MQ=255)
                                                                                                                                        gTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc              >  2:1600736/1‑149 (MQ=255)
                                                                                                                                        gTTCATCAAAGCGCGCCAGACGGGCTGTACCTTTCGACTGTCGGCCGGTCGGCCCTTGACGGACCCCTGCCAGCGCTGCCTCAATCGACTTACGACGCGCCGCTTCTTGGGACCCTTCCTGCGCCAGGCGCTGATCGTTCTGCCcccgc              <  1:105503/149‑1 (MQ=255)
                                                                                                                                         ttcttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcc             >  1:1539138/1‑149 (MQ=255)
                                                                                                                                            ttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCagg          >  1:666955/1‑149 (MQ=255)
                                                                                                                                               aaaGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggagg       >  1:694526/1‑149 (MQ=255)
                                                                                                                                                aaGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCAATCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAggagga      <  1:990345/149‑1 (MQ=255)
                                                                                                                                                 aGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGgaggag     <  1:1515751/149‑1 (MQ=255)
                                                                                                                                                 aGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGgaggag     <  2:806543/149‑1 (MQ=255)
                                                                                                                                                  gcgcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAGTCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTGTCTGCTCCAGCCAGGAGGagt    >  2:304633/1‑149 (MQ=255)
                                                                                                                                                  gcgcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt    >  1:1462554/1‑149 (MQ=255)
                                                                                                                                                   ggcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACGCATTCCATCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGATTATCTTTCTGCTCCAGCCAGGAGGagta   <  2:794760/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAG  >  NC_000913/4629498‑4629794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: