Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,629,649 C→T 7.7% A292T (GCG→ACG)  ettA ← energy‑dependent translational throttle A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,629,6490CT 7.7% 305.9 / 9.3 103A292T (GCG→ACG) ettAenergy‑dependent translational throttle A
Reads supporting (aligned to +/- strand):  ref base C (46/48);  new base T (2/6);  total (49/54)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 109 total aligned reads displayed.

CATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTT  >  NC_000913/4629502‑4629796
                                                                                                                                                   |                                                                                                                                                   
catcaATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGcc                                                                                                                                                    >  2:19975/1‑149 (MQ=255)
 atcaatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCATAGCGCGCCa                                                                                                                                                   >  1:131596/1‑149 (MQ=255)
    aatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCCGCCCTTTATCGCCCAGACGCGGTCGAGGTGGAATACGCAGTTCGTTGGTTGCGTGACGTTTCTGATATTCGGTGCTGTTCAGTTCTTACATGCGCCCCacac                                                                                                                                                >  2:105503/1‑149 (MQ=255)
    aatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGAATTCAAGCACTTTATCGCCCAGACGCGTTACAGGTGGAATAAACAGTTCGATGGTTTCGTTACCTTTCTGATATTCGGTGCGGTTCAGTTCTTCAAAGCGCGACAGAc                                                                                                                                                >  2:105508/1‑149 (MQ=255)
        tgcatACGATCGCCATCGGATTTACGCAGGTGGCTGACTTCGCGCACTTTATCGCCCAGACGCGGGGCATCGGGAATAAACAGTTCGTGGGTTGCGTTACGTTTCTGATATTCGGTGCTGTGCAGTTCTTCAAAGCGCGCCAGACGTGc                                                                                                                                            <  1:1215284/148‑1 (MQ=255)
        cgcagACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGc                                                                                                                                            <  1:1437242/148‑1 (MQ=255)
         gcagACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGCCGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCt                                                                                                                                           >  2:485394/1‑149 (MQ=255)
           agACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCttt                                                                                                                                         >  2:1099084/1‑149 (MQ=255)
             aCGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTAc                                                                                                                                       <  2:166352/149‑1 (MQ=255)
             aCGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttga                                                                                                                                       <  2:897263/149‑3 (MQ=255)
              cGATCGCCATAGGCAGTCCGCACGTTGCCGACCTCCAGCACTTTATCGCCCACCCGCGGACCAGTAGGAATAAACAGTTCGTTGGTTTCGCGACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                      <  1:1079360/149‑4 (MQ=255)
              cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACTCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                      <  2:797126/149‑4 (MQ=255)
              cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTAcc                                                                                                                                      <  1:368329/149‑1 (MQ=255)
              cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                      <  2:375411/149‑4 (MQ=255)
                ctCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCtt                                                                                                                                    <  2:1288849/148‑1 (MQ=255)
                aTCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCACCAGACGTGCTTTACCtt                                                                                                                                    >  1:1208071/1‑149 (MQ=255)
                 tCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaagaa                                                                                                                                   <  1:1565960/149‑7 (MQ=255)
                    ccATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGa                                                                                                                                <  2:1296346/149‑1 (MQ=255)
                     cATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGAc                                                                                                                               >  1:627/1‑149 (MQ=255)
                      aTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACt                                                                                                                              >  2:1515751/1‑149 (MQ=255)
                       tAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCttt                                                                                                                                         >  1:1309776/1‑137 (MQ=255)
                       tAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCttt                                                                                                                                         <  2:1309776/137‑1 (MQ=255)
                               aCGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCtt                                                                                                                     <  2:705271/149‑1 (MQ=255)
                                  cAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAg                                                                                                                  <  2:563046/149‑1 (MQ=255)
                                  cAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAg                                                                                                                  >  2:639359/1‑149 (MQ=255)
                                   aGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGt                                                                                                                 <  1:1552472/149‑1 (MQ=255)
                                   aGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTGTCGTTACGTTTCTGATATAAGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTATACGTTTCGACTGACGGCCTTTAGt                                                                                                                 >  1:784408/1‑149 (MQ=255)
                                     gTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTAc                                                                                                               >  2:317597/1‑149 (MQ=255)
                                        gCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                            <  2:1076862/149‑1 (MQ=255)
                                          tGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTgc                                                                                                          >  2:1338187/1‑148 (MQ=255)
                                           gACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGAc                                                                                                         <  1:668776/149‑1 (MQ=255)
                                             cTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGt                                                                                                       <  2:1370934/149‑1 (MQ=255)
                                              ttCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATTTTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACGTTGACGTa                                                                                                      >  2:243539/1‑149 (MQ=255)
                                                   gCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                            <  2:792080/138‑1 (MQ=255)
                                                   gCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                            >  1:792080/1‑138 (MQ=255)
                                                     aCTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                               <  1:1108549/149‑1 (MQ=255)
                                                           tCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGctc                                                                                         >  1:1226286/1‑149 (MQ=255)
                                                              cccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCTTTTAGTACCTTGACGTACCCATTCCAGCTCttt                                                                                      >  1:245587/1‑149 (MQ=255)
                                                              cccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCttt                                                                                      >  2:1580657/1‑149 (MQ=255)
                                                              cccAGACGCGGTACAGGTGGAATAAACAGTTCGTTGGTATCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACCTGATTAACCTTTCGACTGACGTCCTTTAGTACCTTGACGTACACATACCAGctctat                                                                                      >  1:519067/1‑147 (MQ=255)
                                                                cAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGACTTTAGTACATTGACGTACCCATTCCAGCTCTTtct                                                                                    >  1:794760/1‑149 (MQ=255)
                                                                   aCGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCACCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCaa                                                                                 <  2:1208071/149‑1 (MQ=255)
                                                                    cgcgGTCGAGGTGGAATAAACAGTTCGTTGGTTGCGTTACGTTTCTGATATTAGGTGCTGTTAAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGTCCTTTAGTACCTTGACGTACACATTCCAGCTCTTTCTCAAt                                                                                <  2:519067/149‑1 (MQ=255)
                                                                    cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACTTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACATTGACGTACCCATTCCAGCTCTTTCTAAAt                                                                                >  1:1018059/1‑149 (MQ=255)
                                                                    cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAAt                                                                                >  1:869586/1‑149 (MQ=255)
                                                                     gcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATc                                                                               >  1:912419/1‑149 (MQ=255)
                                                                     gcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATc                                                                               <  1:460277/149‑1 (MQ=255)
                                                                        gTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGAGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGAc                                                                            >  2:286710/1‑149 (MQ=255)
                                                                         tCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACt                                                                           <  2:1050955/149‑1 (MQ=255)
                                                                           cAGGTGGAAGACAGAGTTCGTTGGGTGCGTTACGTTTCTGTTATTCGGTGTTGTTCAGTTCTTCAAAGCGCGCCCCACGTTCTTTACCTTTCGCCTGACGGCCTTTCGTACCTTGACGTACCCATTCCAGCGCTTGCTCACTCGACTTa                                                                         <  2:1057784/149‑1 (MQ=255)
                                                                            aGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTac                                                                        >  2:176825/1‑149 (MQ=255)
                                                                                  aaTAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGAcgcg                                                                  >  1:1106058/1‑149 (MQ=255)
                                                                                   aTAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACgcgc                                                                 >  1:1573517/1‑149 (MQ=255)
                                                                                      aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTTCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTTCCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                              >  2:475758/1‑149 (MQ=255)
                                                                                      aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                              <  1:839508/149‑1 (MQ=255)
                                                                                      aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                              <  2:340884/149‑1 (MQ=255)
                                                                                       aCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGct                                                             >  1:476677/1‑149 (MQ=255)
                                                                                            tCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTtg                                                        <  1:770598/149‑1 (MQ=255)
                                                                                              gTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTtgtg                                                      <  2:912419/149‑1 (MQ=255)
                                                                                                tGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGaa                                                    <  2:476677/149‑1 (MQ=255)
                                                                                                tGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGaa                                                    <  1:176825/149‑1 (MQ=255)
                                                                                                     tCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTc                                               <  2:809432/149‑1 (MQ=255)
                                                                                                          aCGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                               <  2:900924/96‑1 (MQ=255)
                                                                                                           cGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                         <  1:1580657/149‑1 (MQ=255)
                                                                                                            gTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                        >  1:559692/1‑149 (MQ=255)
                                                                                                            gTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                        <  1:113335/149‑1 (MQ=255)
                                                                                                            gTTGCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                        <  2:275574/149‑1 (MQ=255)
                                                                                                                 tGATATTCGGTGCTGTTCAGTTCTTCAAATCGCGCCAGACGTGCTTTACCGTTCGACTGACGGCCTTTAGTAACTTGAAGTACCCATTCCAGCTATGTCTAAAACGACTTACGACGCGACGCTTCTTGTGAAGCTTCCTCCGCCATgcg                                   >  2:1388342/1‑149 (MQ=255)
                                                                                                                 tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCCGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGAGAAGCTTCCTGCTCCCGgcg                                   >  2:1045115/1‑149 (MQ=255)
                                                                                                                 tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGCCTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgcg                                   >  2:1050428/1‑149 (MQ=255)
                                                                                                                 tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgcg                                   >  1:143607/1‑149 (MQ=255)
                                                                                                                  gATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTATACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCTGCTCTTTCTCAATCGACTTACGACGCGCCTCTTCTTGTGAAGCTTCCTGCGCCAGgcgc                                  <  2:784408/149‑1 (MQ=255)
                                                                                                                   atatTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                 <  1:485394/149‑1 (MQ=255)
                                                                                                                   atatTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                 >  1:399475/1‑149 (MQ=255)
                                                                                                                        cGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCt                            <  2:547632/149‑1 (MQ=255)
                                                                                                                          gTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCATCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCttt                          <  1:243539/149‑1 (MQ=255)
                                                                                                                          gTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCttt                          <  1:639359/149‑1 (MQ=255)
                                                                                                                           tGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTAGACTGACGGCCTTTAGTACCTTTACTTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                         <  2:1061073/149‑1 (MQ=255)
                                                                                                                           ggCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGGGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                         <  1:322575/148‑1 (MQ=255)
                                                                                                                           ggCTGTTCAGTGCTTCACAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                         <  2:836879/148‑1 (MQ=255)
                                                                                                                            gCTGTTCAGTTCTTCATAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCTTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCt                        <  2:245587/149‑1 (MQ=255)
                                                                                                                            gCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCTGGCGCTGATCTTTCt                        <  2:1415294/149‑1 (MQ=255)
                                                                                                                             cTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTg                       <  2:581547/149‑1 (MQ=255)
                                                                                                                               gTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCt                     <  2:1573517/149‑1 (MQ=255)
                                                                                                                                   aGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccag                 >  2:1298237/1‑149 (MQ=255)
                                                                                                                                    gaaaggtaaaGCACGTCTGGCGCGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc                >  2:1137341/8‑149 (MQ=255)
                                                                                                                                    gTTCTTCTAAGCGAGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTAGCTTGACGGACCCATTCGAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTAGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc                <  1:105508/149‑1 (MQ=255)
                                                                                                                                    gTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc                >  2:1600736/1‑149 (MQ=255)
                                                                                                                                    gTTCATCAAAGCGCGCCAGACGGGCTGTACCTTTCGACTGTCGGCCGGTCGGCCCTTGACGGACCCCTGCCAGCGCTGCCTCAATCGACTTACGACGCGCCGCTTCTTGGGACCCTTCCTGCGCCAGGCGCTGATCGTTCTGCCcccgc                <  1:105503/149‑1 (MQ=255)
                                                                                                                                     ttcttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcc               >  1:1539138/1‑149 (MQ=255)
                                                                                                                                        ttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCagg            >  1:1536762/1‑149 (MQ=255)
                                                                                                                                        ttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCagg            >  1:666955/1‑149 (MQ=255)
                                                                                                                                           aaaGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggagg         >  1:694526/1‑149 (MQ=255)
                                                                                                                                            aaGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCAATCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAggagga        <  1:990345/149‑1 (MQ=255)
                                                                                                                                             aGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGgaggag       <  1:1515751/149‑1 (MQ=255)
                                                                                                                                             aGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGgaggag       <  2:806543/149‑1 (MQ=255)
                                                                                                                                              gcgcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt      >  1:1462554/1‑149 (MQ=255)
                                                                                                                                               ggcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACGCATTCCATCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGATTATCTTTCTGCTCCAGCCAGGAGGagta     <  2:794760/148‑1 (MQ=255)
                                                                                                                                                gcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagtag    <  2:1205759/149‑1 (MQ=255)
                                                                                                                                                  gcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGtt  >  1:730779/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTT  >  NC_000913/4629502‑4629796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: