Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,629,651 A→T 7.6% L291Q (CTG→CAG)  ettA ← energy‑dependent translational throttle A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,629,6510AT 7.6% 308.7 / 12.7 106L291Q (CTG→CAG) ettAenergy‑dependent translational throttle A
Reads supporting (aligned to +/- strand):  ref base A (48/49);  new base T (2/6);  total (50/56)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

Only 100 of 109 total aligned reads displayed.

ATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACC  >  NC_000913/4629503‑4629799
                                                                                                                                                    |                                                                                                                                                    
atcaatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCATAGCGCGCCa                                                                                                                                                      >  1:131596/1‑149 (MQ=255)
 tcaatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAg                                                                                                                                                     >  2:668776/1‑149 (MQ=255)
   aatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCCGCCCTTTATCGCCCAGACGCGGTCGAGGTGGAATACGCAGTTCGTTGGTTGCGTGACGTTTCTGATATTCGGTGCTGTTCAGTTCTTACATGCGCCCCacac                                                                                                                                                   >  2:105503/1‑149 (MQ=255)
   aatcaGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGAATTCAAGCACTTTATCGCCCAGACGCGTTACAGGTGGAATAAACAGTTCGATGGTTTCGTTACCTTTCTGATATTCGGTGCGGTTCAGTTCTTCAAAGCGCGACAGAc                                                                                                                                                   >  2:105508/1‑149 (MQ=255)
       tgcatACGATCGCCATCGGATTTACGCAGGTGGCTGACTTCGCGCACTTTATCGCCCAGACGCGGGGCATCGGGAATAAACAGTTCGTGGGTTGCGTTACGTTTCTGATATTCGGTGCTGTGCAGTTCTTCAAAGCGCGCCAGACGTGc                                                                                                                                               <  1:1215284/148‑1 (MQ=255)
       cgcagACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGc                                                                                                                                               <  1:1437242/148‑1 (MQ=255)
        gcagACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGCCGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCt                                                                                                                                              >  2:485394/1‑149 (MQ=255)
          agACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCttt                                                                                                                                            >  2:1099084/1‑149 (MQ=255)
            aCGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTAc                                                                                                                                          <  2:166352/149‑1 (MQ=255)
            aCGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttga                                                                                                                                          <  2:897263/149‑3 (MQ=255)
             cGATCGCCATAGGCAGTCCGCACGTTGCCGACCTCCAGCACTTTATCGCCCACCCGCGGACCAGTAGGAATAAACAGTTCGTTGGTTTCGCGACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                         <  1:1079360/149‑4 (MQ=255)
             cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACTCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                         <  2:797126/149‑4 (MQ=255)
             cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTAcc                                                                                                                                         <  1:368329/149‑1 (MQ=255)
             cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                         <  2:375411/149‑4 (MQ=255)
               ctCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCtt                                                                                                                                       <  2:1288849/148‑1 (MQ=255)
               aTCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCACCAGACGTGCTTTACCtt                                                                                                                                       >  1:1208071/1‑149 (MQ=255)
                tCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaagaa                                                                                                                                      <  1:1565960/149‑7 (MQ=255)
                   ccATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGa                                                                                                                                   <  2:1296346/149‑1 (MQ=255)
                    cATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGAc                                                                                                                                  >  1:627/1‑149 (MQ=255)
                     aTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACt                                                                                                                                 >  2:1515751/1‑149 (MQ=255)
                      tAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCttt                                                                                                                                            <  2:1309776/137‑1 (MQ=255)
                      tAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCttt                                                                                                                                            >  1:1309776/1‑137 (MQ=255)
                                 cAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAg                                                                                                                     <  2:563046/149‑1 (MQ=255)
                                 cAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAg                                                                                                                     >  2:639359/1‑149 (MQ=255)
                                  aGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGt                                                                                                                    <  1:1552472/149‑1 (MQ=255)
                                  aGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTGTCGTTACGTTTCTGATATAAGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTATACGTTTCGACTGACGGCCTTTAGt                                                                                                                    >  1:784408/1‑149 (MQ=255)
                                       gCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                               <  2:1076862/149‑1 (MQ=255)
                                         tGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTgc                                                                                                             >  2:1338187/1‑148 (MQ=255)
                                          gACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGAc                                                                                                            <  1:668776/149‑1 (MQ=255)
                                            cTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGt                                                                                                          <  2:1370934/149‑1 (MQ=255)
                                             ttCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATTTTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACGTTGACGTa                                                                                                         >  2:243539/1‑149 (MQ=255)
                                                  gCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                               >  1:792080/1‑138 (MQ=255)
                                                  gCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                               <  2:792080/138‑1 (MQ=255)
                                                    aCTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                                  <  1:1108549/149‑1 (MQ=255)
                                                          tCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGctc                                                                                            >  1:1226286/1‑149 (MQ=255)
                                                             cccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCTTTTAGTACCTTGACGTACCCATTCCAGCTCttt                                                                                         >  1:245587/1‑149 (MQ=255)
                                                             cccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCttt                                                                                         >  2:1580657/1‑149 (MQ=255)
                                                             cccAGACGCGGTACAGGTGGAATAAACAGTTCGTTGGTATCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACCTGATTAACCTTTCGACTGACGTCCTTTAGTACCTTGACGTACACATACCAGctctat                                                                                         >  1:519067/1‑147 (MQ=255)
                                                               cAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGACTTTAGTACATTGACGTACCCATTCCAGCTCTTtct                                                                                       >  1:794760/1‑149 (MQ=255)
                                                                  aCGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCACCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCaa                                                                                    <  2:1208071/149‑1 (MQ=255)
                                                                   cgcgGTCGAGGTGGAATAAACAGTTCGTTGGTTGCGTTACGTTTCTGATATTAGGTGCTGTTAAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGTCCTTTAGTACCTTGACGTACACATTCCAGCTCTTTCTCAAt                                                                                   <  2:519067/149‑1 (MQ=255)
                                                                   cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACTTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACATTGACGTACCCATTCCAGCTCTTTCTAAAt                                                                                   >  1:1018059/1‑149 (MQ=255)
                                                                   cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAAt                                                                                   >  1:869586/1‑149 (MQ=255)
                                                                    gcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATc                                                                                  >  1:912419/1‑149 (MQ=255)
                                                                    gcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATc                                                                                  <  1:460277/149‑1 (MQ=255)
                                                                       gTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGAGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGAc                                                                               >  2:286710/1‑149 (MQ=255)
                                                                        tCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACt                                                                              <  2:1050955/149‑1 (MQ=255)
                                                                          cAGGTGGAAGACAGAGTTCGTTGGGTGCGTTACGTTTCTGTTATTCGGTGTTGTTCAGTTCTTCAAAGCGCGCCCCACGTTCTTTACCTTTCGCCTGACGGCCTTTCGTACCTTGACGTACCCATTCCAGCGCTTGCTCACTCGACTTa                                                                            <  2:1057784/149‑1 (MQ=255)
                                                                           aGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTac                                                                           >  2:176825/1‑149 (MQ=255)
                                                                                 aaTAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGAcgcg                                                                     >  1:1106058/1‑149 (MQ=255)
                                                                                  aTAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACgcgc                                                                    >  1:1573517/1‑149 (MQ=255)
                                                                                     aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTTCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTTCCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                                 >  2:475758/1‑149 (MQ=255)
                                                                                     aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                                 <  1:839508/149‑1 (MQ=255)
                                                                                     aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                                 <  2:340884/149‑1 (MQ=255)
                                                                                      aCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGct                                                                >  1:476677/1‑149 (MQ=255)
                                                                                           tCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTtg                                                           <  1:770598/149‑1 (MQ=255)
                                                                                             gTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTtgtg                                                         <  2:912419/149‑1 (MQ=255)
                                                                                               tGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGaa                                                       <  2:476677/149‑1 (MQ=255)
                                                                                               tGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGaa                                                       <  1:176825/149‑1 (MQ=255)
                                                                                                    tCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTc                                                  <  2:809432/149‑1 (MQ=255)
                                                                                                         aCGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                                  <  2:900924/96‑1 (MQ=255)
                                                                                                          cGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                            <  1:1580657/149‑1 (MQ=255)
                                                                                                           gTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                           <  1:113335/149‑1 (MQ=255)
                                                                                                           gTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                           >  1:559692/1‑149 (MQ=255)
                                                                                                           gTTGCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                           <  2:275574/149‑1 (MQ=255)
                                                                                                                tGATATTCGGTGCTGTTCAGTTCTTCAAATCGCGCCAGACGTGCTTTACCGTTCGACTGACGGCCTTTAGTAACTTGAAGTACCCATTCCAGCTATGTCTAAAACGACTTACGACGCGACGCTTCTTGTGAAGCTTCCTCCGCCATgcg                                      >  2:1388342/1‑149 (MQ=255)
                                                                                                                tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCCGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGAGAAGCTTCCTGCTCCCGgcg                                      >  2:1045115/1‑149 (MQ=255)
                                                                                                                tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGCCTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgcg                                      >  2:1050428/1‑149 (MQ=255)
                                                                                                                tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgcg                                      >  1:143607/1‑149 (MQ=255)
                                                                                                                 gATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTATACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCTGCTCTTTCTCAATCGACTTACGACGCGCCTCTTCTTGTGAAGCTTCCTGCGCCAGgcgc                                     <  2:784408/149‑1 (MQ=255)
                                                                                                                  atatTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                    <  1:485394/149‑1 (MQ=255)
                                                                                                                  atatTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                    >  1:399475/1‑149 (MQ=255)
                                                                                                                       cGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCt                               <  2:547632/149‑1 (MQ=255)
                                                                                                                         gTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCATCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCttt                             <  1:243539/149‑1 (MQ=255)
                                                                                                                         gTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCttt                             <  1:639359/149‑1 (MQ=255)
                                                                                                                          tGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTAGACTGACGGCCTTTAGTACCTTTACTTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                            <  2:1061073/149‑1 (MQ=255)
                                                                                                                          ggCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGGGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                            <  1:322575/148‑1 (MQ=255)
                                                                                                                          ggCTGTTCAGTGCTTCACAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                            <  2:836879/148‑1 (MQ=255)
                                                                                                                           gCTGTTCAGTTCTTCATAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCTTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCt                           <  2:245587/149‑1 (MQ=255)
                                                                                                                           gCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCTGGCGCTGATCTTTCt                           <  2:1415294/149‑1 (MQ=255)
                                                                                                                            cTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTg                          <  2:581547/149‑1 (MQ=255)
                                                                                                                              gTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCt                        <  2:1573517/149‑1 (MQ=255)
                                                                                                                                  aGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccag                    >  2:1298237/1‑149 (MQ=255)
                                                                                                                                   gaaaggtaaaGCACGTCTGGCGCGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc                   >  2:1137341/8‑149 (MQ=255)
                                                                                                                                   gTTCTTCTAAGCGAGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTAGCTTGACGGACCCATTCGAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTAGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc                   <  1:105508/149‑1 (MQ=255)
                                                                                                                                   gTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc                   >  2:1600736/1‑149 (MQ=255)
                                                                                                                                   gTTCATCAAAGCGCGCCAGACGGGCTGTACCTTTCGACTGTCGGCCGGTCGGCCCTTGACGGACCCCTGCCAGCGCTGCCTCAATCGACTTACGACGCGCCGCTTCTTGGGACCCTTCCTGCGCCAGGCGCTGATCGTTCTGCCcccgc                   <  1:105503/149‑1 (MQ=255)
                                                                                                                                    ttcttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagcc                  >  1:1539138/1‑149 (MQ=255)
                                                                                                                                       ttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCagg               >  1:1536762/1‑149 (MQ=255)
                                                                                                                                       ttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCagg               >  1:666955/1‑149 (MQ=255)
                                                                                                                                          aaaGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggagg            >  1:694526/1‑149 (MQ=255)
                                                                                                                                           aaGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCAATCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAggagga           <  1:990345/149‑1 (MQ=255)
                                                                                                                                            aGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGgaggag          <  1:1515751/149‑1 (MQ=255)
                                                                                                                                            aGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGgaggag          <  2:806543/149‑1 (MQ=255)
                                                                                                                                             gcgcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAGTCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTGTCTGCTCCAGCCAGGAGGagt         >  2:304633/1‑149 (MQ=255)
                                                                                                                                             gcgcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt         >  1:1462554/1‑149 (MQ=255)
                                                                                                                                              ggcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACGCATTCCATCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGATTATCTTTCTGCTCCAGCCAGGAGGagta        <  2:794760/148‑1 (MQ=255)
                                                                                                                                               gcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagtag       <  2:1205759/149‑1 (MQ=255)
                                                                                                                                                 gcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGtt     >  1:730779/1‑149 (MQ=255)
                                                                                                                                                    aGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTAcc  >  2:790404/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACC  >  NC_000913/4629503‑4629799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: