Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,629,656 T→C 7.7% A289A (GCA→GCG ettA ← energy‑dependent translational throttle A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,629,6560TC 7.7% 304.3 / 11.9 106A289A (GCA→GCGettAenergy‑dependent translational throttle A
Reads supporting (aligned to +/- strand):  ref base T (46/50);  new base C (2/6);  total (49/57)
Fisher's exact test for biased strand distribution p-value = 2.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 106 total aligned reads displayed.

AGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTT  >  NC_000913/4629510‑4629801
                                                                                                                                                  |                                                                                                                                                 
tgcatACGATCGCCATCGGATTTACGCAGGTGGCTGACTTCGCGCACTTTATCGCCCAGACGCGGGGCATCGGGAATAAACAGTTCGTGGGTTGCGTTACGTTTCTGATATTCGGTGCTGTGCAGTTCTTCAAAGCGCGCCAGACGTGc                                                                                                                                                 <  1:1215284/148‑1 (MQ=255)
cgcagACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGc                                                                                                                                                 <  1:1437242/148‑1 (MQ=255)
 gcagACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGCCGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCt                                                                                                                                                >  2:485394/1‑149 (MQ=255)
   agACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCttt                                                                                                                                              >  2:1099084/1‑149 (MQ=255)
     aCGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTAc                                                                                                                                            <  2:166352/149‑1 (MQ=255)
     aCGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttga                                                                                                                                            <  2:897263/149‑3 (MQ=255)
      cGATCGCCATAGGCAGTCCGCACGTTGCCGACCTCCAGCACTTTATCGCCCACCCGCGGACCAGTAGGAATAAACAGTTCGTTGGTTTCGCGACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                           <  1:1079360/149‑4 (MQ=255)
      cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACTCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                           <  2:797126/149‑4 (MQ=255)
      cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTAcc                                                                                                                                           <  1:368329/149‑1 (MQ=255)
      cGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaa                                                                                                                                           <  2:375411/149‑4 (MQ=255)
        ctCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCtt                                                                                                                                         <  2:1288849/148‑1 (MQ=255)
        aTCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCACCAGACGTGCTTTACCtt                                                                                                                                         >  1:1208071/1‑149 (MQ=255)
         tCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCtttgaagaa                                                                                                                                        <  1:1565960/149‑7 (MQ=255)
            ccATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGa                                                                                                                                     <  2:1296346/149‑1 (MQ=255)
             cATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGAc                                                                                                                                    >  1:627/1‑149 (MQ=255)
               tAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCttt                                                                                                                                              <  2:1309776/137‑1 (MQ=255)
               tAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCACGTCTGGCGCGCttt                                                                                                                                              >  1:1309776/1‑137 (MQ=255)
                       aCGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCtt                                                                                                                          <  2:705271/149‑1 (MQ=255)
                         gCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGGTCGATGGTTTCGTGACGTGACTGAGATTCGGGGCTGTTCAGTTCTTCACAGCGCGCCAGACGTGCTTTACCTTTAGACGGACGGCCtttt                                                                                                                        >  1:219830/1‑148 (MQ=255)
                          cAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAg                                                                                                                       <  2:563046/149‑1 (MQ=255)
                          cAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAg                                                                                                                       >  2:639359/1‑149 (MQ=255)
                           aGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGt                                                                                                                      <  1:1552472/149‑1 (MQ=255)
                           aGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTGTCGTTACGTTTCTGATATAAGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTATACGTTTCGACTGACGGCCTTTAGt                                                                                                                      >  1:784408/1‑149 (MQ=255)
                             gTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTAc                                                                                                                    >  2:317597/1‑149 (MQ=255)
                                gCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                                 <  2:1076862/149‑1 (MQ=255)
                                  tGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTgc                                                                                                               >  2:1338187/1‑148 (MQ=255)
                                   gACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGAc                                                                                                              <  1:668776/149‑1 (MQ=255)
                                     cTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGt                                                                                                            <  2:1370934/149‑1 (MQ=255)
                                      ttCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATTTTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACGTTGACGTa                                                                                                           >  2:243539/1‑149 (MQ=255)
                                           gCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                                 >  1:792080/1‑138 (MQ=255)
                                           gCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCtt                                                                                                                 <  2:792080/138‑1 (MQ=255)
                                             aCTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                                    <  1:1108549/149‑1 (MQ=255)
                                                  aTCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGct                                                                                               >  2:113335/1‑149 (MQ=255)
                                                   tCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGctc                                                                                              >  1:1226286/1‑149 (MQ=255)
                                                      cccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCTTTTAGTACCTTGACGTACCCATTCCAGCTCttt                                                                                           >  1:245587/1‑149 (MQ=255)
                                                      cccAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCttt                                                                                           >  2:1580657/1‑149 (MQ=255)
                                                      cccAGACGCGGTACAGGTGGAATAAACAGTTCGTTGGTATCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACCTGATTAACCTTTCGACTGACGTCCTTTAGTACCTTGACGTACACATACCAGctctat                                                                                           >  1:519067/1‑147 (MQ=255)
                                                        cAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGACTTTAGTACATTGACGTACCCATTCCAGCTCTTtct                                                                                         >  1:794760/1‑149 (MQ=255)
                                                           aCGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCACCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCaa                                                                                      <  2:1208071/149‑1 (MQ=255)
                                                            cgcgGTCGAGGTGGAATAAACAGTTCGTTGGTTGCGTTACGTTTCTGATATTAGGTGCTGTTAAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGTCCTTTAGTACCTTGACGTACACATTCCAGCTCTTTCTCAAt                                                                                     <  2:519067/149‑1 (MQ=255)
                                                            cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACTTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACATTGACGTACCCATTCCAGCTCTTTCTAAAt                                                                                     >  1:1018059/1‑149 (MQ=255)
                                                            cgcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAAt                                                                                     >  1:869586/1‑149 (MQ=255)
                                                             gcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATc                                                                                    >  1:912419/1‑149 (MQ=255)
                                                             gcgGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATc                                                                                    <  1:460277/149‑1 (MQ=255)
                                                                gTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGAGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGAc                                                                                 >  2:286710/1‑149 (MQ=255)
                                                                   cAGGTGGAAGACAGAGTTCGTTGGGTGCGTTACGTTTCTGTTATTCGGTGTTGTTCAGTTCTTCAAAGCGCGCCCCACGTTCTTTACCTTTCGCCTGACGGCCTTTCGTACCTTGACGTACCCATTCCAGCGCTTGCTCACTCGACTTa                                                                              <  2:1057784/149‑1 (MQ=255)
                                                                    aGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTac                                                                             >  2:176825/1‑149 (MQ=255)
                                                                          aaTAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGAcgcg                                                                       >  1:1106058/1‑149 (MQ=255)
                                                                           aTAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACgcgc                                                                      >  1:1573517/1‑149 (MQ=255)
                                                                              aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTTCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTTCCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                                   >  2:475758/1‑149 (MQ=255)
                                                                              aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                                   <  1:839508/149‑1 (MQ=255)
                                                                              aaCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGcgccgc                                                                   <  2:340884/149‑1 (MQ=255)
                                                                               aCAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGct                                                                  >  1:476677/1‑149 (MQ=255)
                                                                                   ttCGGTGGTTTCGTTACGTTTGTGATATTCGGTGCTGATCAGTTCTTCAAAGCCCGCCAGCCGTGCGTTACCTTTCCACTGACGGCCTTTTGTACCTTGCCGTACCCAATCCATCTGTTTGTTATTCGACTTACGACGCGCCGcttctt                                                              <  1:1395133/149‑1 (MQ=255)
                                                                                    tCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTtg                                                             <  1:770598/149‑1 (MQ=255)
                                                                                      gTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTtgtg                                                           <  2:912419/149‑1 (MQ=255)
                                                                                        tGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGaa                                                         <  1:176825/149‑1 (MQ=255)
                                                                                        tGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGaa                                                         <  2:476677/149‑1 (MQ=255)
                                                                                             tCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTc                                                    <  2:809432/149‑1 (MQ=255)
                                                                                                  aCGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                                    <  2:900924/96‑1 (MQ=255)
                                                                                                  aCGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTc                                                                                                    >  1:900924/1‑96 (MQ=255)
                                                                                                   cGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc                                              <  1:1580657/149‑1 (MQ=255)
                                                                                                    gTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                             >  1:559692/1‑149 (MQ=255)
                                                                                                    gTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                             <  1:113335/149‑1 (MQ=255)
                                                                                                    gTTGCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc                                             <  2:275574/149‑1 (MQ=255)
                                                                                                       tCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAgg                                          >  2:146152/1‑149 (MQ=255)
                                                                                                         tGATATTCGGTGCTGTTCAGTTCTTCAAATCGCGCCAGACGTGCTTTACCGTTCGACTGACGGCCTTTAGTAACTTGAAGTACCCATTCCAGCTATGTCTAAAACGACTTACGACGCGACGCTTCTTGTGAAGCTTCCTCCGCCATgcg                                        >  2:1388342/1‑149 (MQ=255)
                                                                                                         tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCCGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGAGAAGCTTCCTGCTCCCGgcg                                        >  2:1045115/1‑149 (MQ=255)
                                                                                                         tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGCCTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgcg                                        >  2:1050428/1‑149 (MQ=255)
                                                                                                         tGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGgcg                                        >  1:143607/1‑149 (MQ=255)
                                                                                                          gATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTATACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCTGCTCTTTCTCAATCGACTTACGACGCGCCTCTTCTTGTGAAGCTTCCTGCGCCAGgcgc                                       <  2:784408/149‑1 (MQ=255)
                                                                                                           atatTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                      <  1:485394/149‑1 (MQ=255)
                                                                                                           atatTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCt                                      >  1:399475/1‑149 (MQ=255)
                                                                                                                  gTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGCCGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCATCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCttt                               <  1:243539/149‑1 (MQ=255)
                                                                                                                  gTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCttt                               <  1:639359/149‑1 (MQ=255)
                                                                                                                   tGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTAGACTGACGGCCTTTAGTACCTTTACTTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                              <  2:1061073/149‑1 (MQ=255)
                                                                                                                   ggCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGGGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                              <  1:322575/148‑1 (MQ=255)
                                                                                                                   ggCTGTTCAGTGCTTCACAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTc                              <  2:836879/148‑1 (MQ=255)
                                                                                                                    gCTGTTCAGTTCTTCATAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCTTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCt                             <  2:245587/149‑1 (MQ=255)
                                                                                                                    gCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCTGGCGCTGATCTTTCt                             <  2:1415294/149‑1 (MQ=255)
                                                                                                                     cTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTg                            <  2:581547/149‑1 (MQ=255)
                                                                                                                       gTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCt                          <  2:1573517/149‑1 (MQ=255)
                                                                                                                           aGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccag                      >  2:1298237/1‑149 (MQ=255)
                                                                                                                            gaaaggtaaaGCACGTCTGGCGCGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc                     >  2:1137341/8‑149 (MQ=255)
                                                                                                                            gTTCTTCTAAGCGAGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTAGCTTGACGGACCCATTCGAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTAGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc                     <  1:105508/149‑1 (MQ=255)
                                                                                                                            gTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTccagc                     >  2:1600736/1‑149 (MQ=255)
                                                                                                                            gTTCATCAAAGCGCGCCAGACGGGCTGTACCTTTCGACTGTCGGCCGGTCGGCCCTTGACGGACCCCTGCCAGCGCTGCCTCAATCGACTTACGACGCGCCGCTTCTTGGGACCCTTCCTGCGCCAGGCGCTGATCGTTCTGCCcccgc                     <  1:105503/149‑1 (MQ=255)
                                                                                                                                ttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCagg                 >  1:1536762/1‑149 (MQ=255)
                                                                                                                                ttcAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCagg                 >  1:666955/1‑149 (MQ=255)
                                                                                                                                   aaaGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggagg              >  1:694526/1‑149 (MQ=255)
                                                                                                                                    aaGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCAATCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAggagga             <  1:990345/149‑1 (MQ=255)
                                                                                                                                     aGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGgaggag            <  1:1515751/149‑1 (MQ=255)
                                                                                                                                     aGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGgaggag            <  2:806543/149‑1 (MQ=255)
                                                                                                                                      gcgcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAGTCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTGTCTGCTCCAGCCAGGAGGagt           >  2:304633/1‑149 (MQ=255)
                                                                                                                                      gcgcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagt           >  1:1462554/1‑149 (MQ=255)
                                                                                                                                       ggcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACGCATTCCATCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGATTATCTTTCTGCTCCAGCCAGGAGGagta          <  2:794760/148‑1 (MQ=255)
                                                                                                                                        gcgcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGagtag         <  2:1205759/149‑1 (MQ=255)
                                                                                                                                          gcCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGtt       >  1:730779/1‑149 (MQ=255)
                                                                                                                                             aGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTAcc    >  2:790404/1‑149 (MQ=255)
                                                                                                                                               aCGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCGCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCtt  <  2:774030/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTT  >  NC_000913/4629510‑4629801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: