Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 507,271 A→C 6.5% noncoding (68/84 nt) chiX → sRNA antisense regulator of OM chitoporin ChiP(YbfM), Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913507,2710AC 6.5% 288.5 / 9.9 92noncoding (68/84 nt)chiXsRNA antisense regulator of OM chitoporin ChiP(YbfM), Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base A (41/45);  new base C (2/4);  total (43/49)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

GCCTGATGGCGGTCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGCACATC  >  NC_000913/507125‑507417
                                                                                                                                                     |                                                                                                                                                  
gCCTGATGGCGGTCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGCGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCGTCTTTGACGGGCCAATTGCGata                                                                                                                                                  >  1:1409952/1‑149 (MQ=255)
    gATGGCGGTCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTgg                                                                                                                                              <  1:1142614/149‑1 (MQ=255)
    gATGGCGGTCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTgg                                                                                                                                              >  2:1456183/1‑149 (MQ=255)
    gATGGCGGTCCAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATATCGCTATTGGc                                                                                                                                             <  2:1250590/149‑1 (MQ=255)
     aTGGCGGTCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATATCGCTATTGGc                                                                                                                                             <  1:207037/149‑1 (MQ=255)
     aTGGCGGTCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGCTACACCGTCGCTTAAAGCGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATATCGCTATTGGc                                                                                                                                             <  1:236312/149‑1 (MQ=255)
      tGGCGGTCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGcc                                                                                                                                            <  1:376394/149‑1 (MQ=255)
       ggCGGTCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATATCGCTATTGGccc                                                                                                                                           <  1:1590426/149‑2 (MQ=255)
           gTCCAAAAAAAAGAGTCATCTTTCCTAAGAGTATTGTCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATTATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCAtttt                                                                                                                                       <  1:225627/149‑1 (MQ=255)
            tCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCAttttt                                                                                                                                      <  1:1440567/149‑1 (MQ=255)
             ccAAAAAAAAGAGTCATCTTGCGTAAGATTATTGGAGGGATGGTGAGATTTAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCAtttttt                                                                                                                                     <  1:103595/149‑1 (MQ=255)
               aaaaaaaaGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTa                                                                                                                                   <  1:631002/149‑1 (MQ=255)
               aaaaaaaaGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTa                                                                                                                                   <  1:485112/149‑1 (MQ=255)
               aaaaaaaaGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACAGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTa                                                                                                                                   <  2:277559/149‑1 (MQ=255)
                     aaGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑AAAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCaa                                                                                                                             <  1:752914/149‑1 (MQ=255)
                          tCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATTAAATTCCTCTTTTACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACAttt                                                                                                                        >  2:169870/1‑149 (MQ=255)
                          tCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACAttt                                                                                                                        <  1:177428/149‑1 (MQ=255)
                          tCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGACGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCGTTGACGGGCATATAGAGATATTGGCCATTTTTTTAGCGCAACAttt                                                                                                                        >  1:840392/1‑149 (MQ=255)
                           cATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTg                                                                                                                       <  1:1435518/149‑1 (MQ=255)
                               ttGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGc                                                                                                                   <  1:474924/149‑1 (MQ=255)
                               ttGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGc                                                                                                                   >  1:267725/1‑149 (MQ=255)
                                  ccTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTAGCCATTTTTTTAGCGCAACATTTGCGGCaaa                                                                                                                <  1:1337816/149‑1 (MQ=255)
                                    tAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAAtt                                                                                                              >  1:383417/1‑149 (MQ=255)
                                    tAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAAtt                                                                                                              <  2:1274619/149‑1 (MQ=255)
                                       gagTATTGGCAGGATGGTGAGATTGGGCGACAATCGAGTTACACCGTCGCTTAAAGTGACTGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTccc                                                                                                           <  1:1090696/149‑1 (MQ=255)
                                       gagTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑TTAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTccc                                                                                                           >  2:1030713/1‑149 (MQ=255)
                                       gagTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTccc                                                                                                           >  2:330475/1‑149 (MQ=255)
                                       gagTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTccc                                                                                                           <  2:916371/149‑1 (MQ=255)
                                         gTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCtt                                                                                                         <  2:267725/149‑1 (MQ=255)
                                          tATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGCATAATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTcca                                                                                                           >  1:689904/1‑148 (MQ=255)
                                          tATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTtc                                                                                                        >  1:895072/1‑149 (MQ=255)
                                            ttGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTtctc                                                                                                      <  2:1554246/149‑1 (MQ=255)
                                            ttGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTtctc                                                                                                      <  2:895072/149‑1 (MQ=255)
                                              ggCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCa                                                                                                    <  1:438402/149‑1 (MQ=255)
                                               gCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCAt                                                                                                   >  1:734689/1‑149 (MQ=255)
                                                 aGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATAc                                                                                                 <  1:1390150/149‑1 (MQ=255)
                                                   gATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAg                                                                                               >  1:902818/1‑149 (MQ=255)
                                                      ggTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGgtg                                                                                            >  2:931474/1‑149 (MQ=255)
                                                      ggTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGgtg                                                                                            >  1:1275955/1‑149 (MQ=255)
                                                       gTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGgtgt                                                                                           >  1:883536/1‑149 (MQ=255)
                                                        tGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTa                                                                                          <  2:297607/149‑1 (MQ=255)
                                                         gagaTTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAg                                                                                         >  1:1013841/1‑149 (MQ=255)
                                                               gAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCCTACACCTGTatggagaa                                                                                   <  2:1118156/149‑8 (MQ=255)
                                                                  cGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCaccgac                                                                                <  1:973702/149‑1 (MQ=255)
                                                                        tCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGAc                                                                          <  1:1030713/149‑1 (MQ=255)
                                                                        tCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGAc                                                                          >  1:596892/1‑149 (MQ=255)
                                                                          gAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACca                                                                        >  2:1316568/1‑149 (MQ=255)
                                                                             ttACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCATATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGCCCACat                                                                     >  2:1076090/1‑149 (MQ=255)
                                                                             ttACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACat                                                                     >  1:1549322/1‑149 (MQ=255)
                                                                               acacCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGAGAAAAATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACatat                                                                   >  2:413698/1‑149 (MQ=255)
                                                                                cacCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATc                                                                  <  1:1456183/149‑1 (MQ=255)
                                                                                    gTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGccc                                                              >  2:645049/1‑149 (MQ=255)
                                                                                         ttAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGAAAATTACCTTCTCCATACAGGTGTAGTGCACCGACCTCGACACCATATAGCCCcggcg                                                         >  1:989724/1‑149 (MQ=255)
                                                                                            aaGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCa                                                      >  2:1030577/1‑149 (MQ=255)
                                                                                             aGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCAt                                                     <  2:1510568/149‑1 (MQ=255)
                                                                                               tGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATgg                                                   <  1:702117/149‑1 (MQ=255)
                                                                                                gACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGTGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGAACACATATCGCCCCGGCGGCATGgc                                                  <  1:971019/149‑1 (MQ=255)
                                                                                                   ggC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGcgcg                                               >  1:892765/1‑149 (MQ=255)
                                                                                                   ggC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGcgcg                                               >  2:1025070/1‑149 (MQ=255)
                                                                                                     c‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTa                                             >  1:37114/1‑149 (MQ=255)
                                                                                                         ataataataAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTaa                                            <  2:511442/149‑1 (MQ=255)
                                                                                                           aataataaAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTTGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATAGCCCCGGCGGCATGCCGCGTAAtt                                          >  1:728396/1‑149 (MQ=255)
                                                                                                           aataataaAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAAtt                                          <  2:466326/149‑1 (MQ=255)
                                                                                                              aataaAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTAt                                       >  2:1199144/1‑149 (MQ=255)
                                                                                                              aataaAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTAt                                       >  2:908836/1‑149 (MQ=255)
                                                                                                               ataaAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATc                                      >  1:163428/1‑149 (MQ=255)
                                                                                                               ataaAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATc                                      <  1:1550568/149‑1 (MQ=255)
                                                                                                                  aaaaaaTGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCg                                   >  2:334355/1‑149 (MQ=255)
                                                                                                                   aaaaaTGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGc                                  <  1:488437/149‑1 (MQ=255)
                                                                                                                          aaaTTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCga                           <  1:447483/149‑1 (MQ=255)
                                                                                                                           aaTTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCgag                          >  2:1333854/1‑149 (MQ=255)
                                                                                                                             ttactctTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGAt                        >  2:580652/4‑149 (MQ=255)
                                                                                                                               ccTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGAttt                      <  2:1275955/149‑1 (MQ=255)
                                                                                                                               ccTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGAttt                      <  1:1062837/149‑1 (MQ=255)
                                                                                                                                ctctTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTc                     >  1:1248534/1‑149 (MQ=255)
                                                                                                                                 tctTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTTTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCg                    <  1:73889/149‑1 (MQ=255)
                                                                                                                                   tttGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCt                  >  1:391789/1‑149 (MQ=255)
                                                                                                                                     tGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGa                <  1:845432/149‑1 (MQ=255)
                                                                                                                                      gACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGAt               <  2:734689/149‑1 (MQ=255)
                                                                                                                                      gACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGAt               <  1:540198/149‑1 (MQ=255)
                                                                                                                                      gACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGAt               <  2:647587/149‑1 (MQ=255)
                                                                                                                                       aCGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATg              >  1:1526371/1‑149 (MQ=255)
                                                                                                                                         tggCCAATATCGCTATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCa            >  1:1491258/2‑149 (MQ=255)
                                                                                                                                         tggCCAATATCGCTATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCa            >  1:528067/2‑149 (MQ=255)
                                                                                                                                         gggCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCa            >  1:24787/1‑149 (MQ=255)
                                                                                                                                         gggCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCa            >  2:544115/1‑149 (MQ=255)
                                                                                                                                           gCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATc          >  1:1559792/1‑149 (MQ=255)
                                                                                                                                              aaTAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGCCCGCGACCACATATCGCCCCGGCGGCATGGCGCGGTATTTATCCCGCCAGGCGAGATTTCGCTGATGCagcagc       >  1:989603/1‑149 (MQ=255)
                                                                                                                                              aaTAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGc       <  2:383417/149‑1 (MQ=255)
                                                                                                                                               aTAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGca      <  2:958067/149‑1 (MQ=255)
                                                                                                                                                   cGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGcgaaat  <  2:902818/149‑6 (MQ=255)
                                                                                                                                                   cGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGCACATc  <  1:750879/149‑1 (MQ=255)
                                                                                                                                                   cGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGCACATc  >  2:1185771/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCCTGATGGCGGTCCAAAAAAAAGAGTCATCTTGCCTAAGAGTATTGGCAGGATGGTGAGATTGAGCGACAATCGAGTTACACCGTCGCTTAAAGTGACGGC‑‑‑ATAATAATAAAAAAATGAAATTCCTCTTTGACGGGCCAATAGCGATATTGGCCATTTTTTTAGCGCAACATTTGCGGCAAATTCCCTTCTCCATACAGGTGTAGTGCACCGACCGCGACCACATATCGCCCCGGCGGCATGGCGCGTAATTTATCCCGCCAGGCGAGATTTCGCTGATGCATCAGCACATC  >  NC_000913/507125‑507417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: