BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	660,180	A→G	5.4%	Y13H (TAC→CAC)	lipA ←	lipoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	660,180	0	A	G	5.4%	346.7 / 6.9	110	Y13H (TAC→CAC)	lipA	lipoate synthase
Reads supporting (aligned to +/- strand): ref base A (48/56); new base G (3/3); total (51/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 114 total aligned reads displayed.

TTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGAC  >  NC_000913/660032‑660328

tttttGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTa                                                                                                                                                      <  1:869016/149‑1 (MQ=255)

tttttGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTa                                                                                                                                                      <  1:1496836/149‑1 (MQ=255)

  tttGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTAtt                                                                                                                                                    <  2:893238/149‑1 (MQ=255)

    tGCTCATTGCGGCTTAGATGCCCTGGATACGTGTAGAGTCCGTTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTa                                                                                                                                                  <  2:770922/149‑1 (MQ=255)

     gcgcATTGCGGCTTTGCTGCCCTGGAGACGTGTAGAGGCCGCTGGAAGCTTGATTTTCATCCATTCCGGCATGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCAGCTTATCGGCATCGCGGTGTTaaa                                                                                                                                                 <  1:448379/149‑1 (MQ=255)

           tGCGGCTTTGATGCCCTGGATACGTGTAGAGTACGCTGGATGCTTGATTTTCATCAATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCTGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACcgc                                                                                                                                           <  2:454988/149‑1 (MQ=255)

              ggCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTGTTAAATACcgcgat                                                                                                                                        <  2:1371170/149‑3 (MQ=255)

              ggCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGtt                                                                                                                                        >  2:1072524/1‑149 (MQ=255)

                cTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTGTTAAATACcgcgatgc                                                                                                                                      <  2:537919/149‑5 (MQ=255)

                  ttGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCAt                                                                                                                                    <  2:814439/149‑1 (MQ=255)

                     aTGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATcac                                                                                                                                 >  1:946952/1‑149 (MQ=255)

                        cccTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAAt                                                                                                                              <  2:1330234/149‑1 (MQ=255)

                        cccTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAAt                                                                                                                              <  1:231886/149‑1 (MQ=255)

                         ccTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATg                                                                                                                             <  1:924971/149‑1 (MQ=255)

                          cTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCCTCACAATgg                                                                                                                            >  2:814103/1‑149 (MQ=255)

                          cTGGATACGGGTAGAGGCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGATTTTACCCGGGATAAGGGCCATCTTAGCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATgg                                                                                                                            <  1:261494/149‑1 (MQ=255)

                                aCGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTAc                                                                                                                      <  2:557918/149‑1 (MQ=255)

                                  gtgtAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTc                                                                                                                    >  2:314232/1‑149 (MQ=255)

                                   tgtAGAGTCCGCTGGAAGCTTGATTTACATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCa                                                                                                                   >  1:733434/1‑149 (MQ=255)

                                     tAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATa                                                                                                                 <  2:585725/149‑1 (MQ=255)

                                     tAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATa                                                                                                                 >  2:702296/1‑149 (MQ=255)

                                       gagTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAgc                                                                                                               >  2:885328/1‑149 (MQ=255)

                                        agTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAgcg                                                                                                              >  2:892620/1‑149 (MQ=255)

                                         gTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGTATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAgcgt                                                                                                             <  1:236423/149‑1 (MQ=255)

                                         gTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAgcgt                                                                                                             <  1:1567314/149‑1 (MQ=255)

                                               tGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTTCgtgt                                                                                                       >  1:1021084/1‑149 (MQ=255)

                                                 gAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTc                                                                                                     <  1:1046697/149‑1 (MQ=255)

                                                 gAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAAAACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTc                                                                                                     <  2:1330736/149‑1 (MQ=255)

                                                  aaGCTTGATTTTCATCCATTCCGGCTTGCGCAGCATGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTcc                                                                                                    <  1:974555/149‑1 (MQ=255)

                                                   aGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCa                                                                                                   <  2:1482862/149‑1 (MQ=255)

                                                   aGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCa                                                                                                   <  1:1023071/149‑1 (MQ=255)

                                                     cTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGt                                                                                                 >  2:146084/1‑149 (MQ=255)

                                                      ttGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGtt                                                                                                >  1:1318530/1‑149 (MQ=255)

                                                        gATTTTCATCCATTCCGGCTTGCGCAGCAGGGGTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCTTCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATATCGTGCGTGTTCCAGTTGc                                                                                              <  1:439816/149‑1 (MQ=255)

                                                        gATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGc                                                                                              >  1:48100/1‑149 (MQ=255)

                                                          ttttCATCCATTCCGGCTTGCGCCGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCCCAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGa                                                                                            >  1:53316/1‑149 (MQ=255)

                                                                tCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACg                                                                                      >  1:830829/1‑149 (MQ=255)

                                                                 ccATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGa                                                                                     >  2:787832/1‑149 (MQ=255)

                                                                  cATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGaa                                                                                    <  2:458712/149‑1 (MQ=255)

                                                                  cATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGaa                                                                                    <  2:116178/149‑1 (MQ=255)

                                                                  cATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGaa                                                                                    <  1:1495247/149‑1 (MQ=255)

                                                                   aTTCCGGCTTGCGCAGCAGGGCTACGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTAACGGCATCGCGGTATTTAACACCGCGTGCCATAACAATGCGTTTACTCATAGCGTCCGTGTTCCAGTTGGGAATTACTAAg                                                                                   >  2:1419741/1‑149 (MQ=255)

                                                                       cGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGaaa                                                                               >  2:194846/1‑149 (MQ=255)

                                                                          cTTGCGCAGCAGGGCTTCGCGCACTGTTGCCACGATTTTAACCCGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCAATCACAATCGGTTTACTCATAGCGTGCGTGTTCAAGTTGCGAATTACGAAGGACAGCg                                                                            >  2:777946/1‑149 (MQ=255)

                                                                             gcgcAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGttt                                                                         <  2:1307382/149‑1 (MQ=255)

                                                                                cagcagGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTttcaa                                                                      <  1:732567/149‑1 (MQ=255)

                                                                                  gcagGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTttcaatt                                                                    <  1:859639/149‑1 (MQ=255)

                                                                                   cagGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTttcaattc                                                                   >  2:824422/1‑149 (MQ=255)

                                                                                   cagGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTttcaattc                                                                   >  1:425652/1‑149 (MQ=255)

                                                                                     gggCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTttcaattcaa                                                                 <  2:73405/149‑1 (MQ=255)

                                                                                      ggCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGTGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTtcaattcaat                                                                >  1:272890/1‑149 (MQ=255)

                                                                                       gCTTCGCGCTCAGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATa                                                               <  2:530374/149‑1 (MQ=255)

                                                                                        cTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTACAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAg                                                              >  1:367506/1‑149 (MQ=255)

                                                                                        cTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATCTAACACCGCGTTCCATCACAATGGGTTTACTCATAGAGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAg                                                              >  1:740548/1‑149 (MQ=255)

                                                                                         ttCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGa                                                             <  2:1451094/149‑1 (MQ=255)

                                                                                         ttCGCGCGCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGa                                                             <  2:218076/149‑1 (MQ=255)

                                                                                             cgcTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGAttgt                                                         >  1:1142431/1‑149 (MQ=255)

                                                                                               ctctGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGAttgttg                                                       <  2:641313/149‑1 (MQ=255)

                                                                                                 ctGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTa                                                     >  1:555361/1‑149 (MQ=255)

                                                                                                 ctGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGGTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTa                                                     <  2:315763/149‑1 (MQ=255)

                                                                                                      gCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTAt                                                <  2:733434/149‑1 (MQ=255)

                                                                                                      gCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTAt                                                >  1:1074823/1‑149 (MQ=255)

                                                                                                      gCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTAAGCATAGCGTGCTTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTAAATTCAATAGATTTTTGTAATTAt                                                >  2:1066267/1‑149 (MQ=255)

                                                                                                       ccACGTTTTTAAGCGGGTTATGGGCCATCTTATCGGCATGGCGGTATTTAACACCGCGTTCCATCTCAATGGGTTTACTCATAGCGTGCTTGTTCCAGTTGCTAATTACGAAGGAAAGCGTATCATTTCAATAGATTGTTGTAATTATc                                               <  1:777946/149‑1 (MQ=255)

                                                                                                       ccACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATc                                               >  2:139711/1‑149 (MQ=255)

                                                                                                       ccACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATc                                               >  2:395055/1‑149 (MQ=255)

                                                                                                         aCGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCaa                                             <  2:1382604/149‑1 (MQ=255)

                                                                                                         aCGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCaa                                             >  1:1517004/1‑149 (MQ=255)

                                                                                                         aCGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCaa                                             <  2:830829/149‑1 (MQ=255)

                                                                                                         aCGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCaa                                             >  2:306578/1‑149 (MQ=255)

                                                                                                                     gggATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAAt                                 >  1:549921/1‑149 (MQ=255)

                                                                                                                        aTAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTaa                              <  1:702296/149‑1 (MQ=255)

                                                                                                                         tAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAAc                             <  1:1432593/149‑1 (MQ=255)

                                                                                                                           aGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGa                           <  1:787832/149‑1 (MQ=255)

                                                                                                                            gggCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTTTCAACTATTTTTGAATTAACGAc                          >  1:1070769/1‑149 (MQ=255)

                                                                                                                            gggCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGAc                          <  2:555361/149‑1 (MQ=255)

                                                                                                                            gggCCATCATATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGAc                          <  1:452003/149‑1 (MQ=255)

                                                                                                                             ggCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACt                         >  2:1273285/1‑149 (MQ=255)

                                                                                                                               ccATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTgg                       <  2:335062/149‑1 (MQ=255)

                                                                                                                                cATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGc                      >  2:1550068/1‑149 (MQ=255)

                                                                                                                                cATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGc                      <  2:1417811/149‑1 (MQ=255)

                                                                                                                                cATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGc                      <  2:1424001/149‑1 (MQ=255)

                                                                                                                                        cGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATc              <  1:194846/149‑1 (MQ=255)

                                                                                                                                         ggCATCGCGGTATTTAACACCTCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCa             <  2:1409346/149‑1 (MQ=255)

                                                                                                                                          ggaacgcgGTGTTAAATACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCAt            >  2:583394/5‑149 (MQ=255)

                                                                                                                                          ggaacgcgGTGTTAAATACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCAt            >  1:551160/5‑149 (MQ=255)

                                                                                                                                          ggaacgcgGTGTTAAATACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCAt            >  1:489993/5‑149 (MQ=255)

                                                                                                                                          gCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCAt            >  1:1126435/1‑149 (MQ=255)

                                                                                                                                           aaTCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAACAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCAtt           <  2:522323/148‑1 (MQ=255)

                                                                                                                                            aTCGCGGTATTTAACACCGCGTTACATCACAATGGGTTTACTAATAGCGGGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTTAATTAACGACTGGAAGTATATCAttt          >  2:1345715/1‑148 (MQ=255)

                                                                                                                                             tCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGa         >  1:314014/1‑149 (MQ=255)

                                                                                                                                             tCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGa         >  2:317521/1‑149 (MQ=255)

                                                                                                                                             tCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGa         >  1:818402/1‑149 (MQ=255)

                                                                                                                                             tCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGa         >  1:21536/1‑149 (MQ=255)

                                                                                                                                               gcgGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGaaa       <  1:892620/149‑1 (MQ=255)

                                                                                                                                               gcgGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGaaa       <  1:314232/149‑1 (MQ=255)

                                                                                                                                               gcgGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGaaa       <  2:1318530/149‑1 (MQ=255)

                                                                                                                                                cgGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAAc      >  2:1220673/1‑149 (MQ=255)

                                                                                                                                                   tATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGa   <  1:814103/149‑1 (MQ=255)

                                                                                                                                                   tATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGa   <  2:1074823/149‑1 (MQ=255)

TTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATTTTCATCCATTCCGGCTTGCGCAGCAGGGCTTCGCGCTCTGTTGCCACGTTTTTAACCGGGATAAGGGCCATCTTATCGGCATCGCGGTATTTAACACCGCGTTCCATCACAATGGGTTTACTCATAGCGTGCGTGTTCCAGTTGCGAATTACGAAGGAAAGCGTTTCAATTCAATAGATTGTTGTAATTATCAACTATTTTTGAATTAACGACTGGCAGTATATCATTGAAACGGAC  >  NC_000913/660032‑660328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`