Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 662,683 A→T 5.1% intergenic (‑41/+69) ybeD ← / ← dacA UPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913662,6830AT 5.1% 427.5 / 9.1 138intergenic (‑41/+69)ybeD/dacAUPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base A (72/59);  new base T (4/3);  total (76/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01

Only 100 of 139 total aligned reads displayed.

TACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGC  >  NC_000913/662538‑662830
                                                                                                                                                 |                                                                                                                                                    
tACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGaa                                                                                                                                                   >  1:655834/1‑149 (MQ=255)
     cTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCgg                                                                                                                                              >  2:215569/1‑149 (MQ=255)
        cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAg                                                                                                                                           <  2:1458565/149‑1 (MQ=255)
        cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                                                                                                                           <  2:1238481/149‑1 (MQ=255)
         aaCCACCTGATCAACCAGCTCAGGTAACGCCTGCCACATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑t                                                                                                                                         >  2:58923/1‑149 (MQ=255)
           ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑ttt                                                                                                                                       <  2:520899/149‑1 (MQ=255)
            caccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAG‑ttgc                                                                                                                                      <  1:427730/149‑3 (MQ=255)
             accTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑ttttt                                                                                                                                     >  2:687450/1‑149 (MQ=255)
             accTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑ttttt                                                                                                                                     <  2:877454/149‑1 (MQ=255)
              ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑tttttt                                                                                                                                    >  1:393949/1‑149 (MQ=255)
                tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTAc                                                                                                                                  >  2:935568/1‑149 (MQ=255)
                 gATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTAcg                                                                                                                                 >  1:908505/1‑148 (MQ=255)
                 gATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACt                                                                                                                                 >  2:735307/1‑149 (MQ=255)
                 gATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACt                                                                                                                                 >  1:162784/1‑149 (MQ=255)
                  aTCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTg                                                                                                                                >  1:1279349/1‑149 (MQ=255)
                  aTCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTg                                                                                                                                >  2:1029036/1‑149 (MQ=255)
                      aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTTCTGATGc                                                                                                                            >  1:247190/1‑149 (MQ=255)
                      aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGc                                                                                                                            >  2:183708/1‑149 (MQ=255)
                       ccAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCt                                                                                                                           <  2:308926/149‑1 (MQ=255)
                          gCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAg                                                                                                                        <  2:607662/149‑1 (MQ=255)
                           cTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCTGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGt                                                                                                                       <  2:1388367/149‑1 (MQ=255)
                            tCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGta                                                                                                                      >  1:882293/1‑149 (MQ=255)
                               ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTatat                                                                                                                   <  1:603953/149‑1 (MQ=255)
                                gTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATg                                                                                                                  >  2:871492/1‑149 (MQ=255)
                                 tAACGCCTGCCCCATAACTTTGAAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCTTAACGTTATTAAATAGCAACTCCCGACAGAACACGGGAC‑TTTTTTACTGATGATTAGTATATgg                                                                                                                 >  2:520184/1‑149 (MQ=255)
                                   aCGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAAAAGCAACTACCGCCAGCAGGAGGGAG‑TATTTTACTGATGCTTAGTATATgggg                                                                                                               >  2:853715/1‑149 (MQ=255)
                                     gCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGAc                                                                                                             <  1:1250597/149‑1 (MQ=255)
                                       cTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACgg                                                                                                           >  2:766457/1‑149 (MQ=255)
                                           cccATAAATTTGTAAGTAAAATGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGAGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAAt                                                                                                       <  2:1232516/149‑1 (MQ=255)
                                            ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAAtt                                                                                                      >  1:875517/1‑149 (MQ=255)
                                             cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAAGTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                     <  2:880081/149‑1 (MQ=255)
                                             cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                     <  1:1456029/149‑1 (MQ=255)
                                             cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                     >  2:1108516/1‑149 (MQ=255)
                                             cATAAATTTTTAAGTAAAAGTAGTAGGGAATTCAAGCAGTTCGTTCAGTTTGGTTTTCATGTCAGCTACGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCTGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                     <  2:1086559/149‑1 (MQ=255)
                                              aTAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACACCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTac                                                                                                    >  1:213317/1‑149 (MQ=255)
                                               tAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTaca                                                                                                   <  1:687450/149‑1 (MQ=255)
                                                   tttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACttt                                                                                               <  1:1061255/149‑1 (MQ=255)
                                                   tttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACttt                                                                                               >  2:401905/1‑149 (MQ=255)
                                                   tttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACttt                                                                                               <  2:393949/149‑1 (MQ=255)
                                                    ttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTc                                                                                              <  2:1456151/149‑1 (MQ=255)
                                                     tGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCG‑TAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCaa                                                                                            <  1:996420/149‑1 (MQ=255)
                                                      gtaagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCaa                                                                                            >  2:824837/1‑149 (MQ=255)
                                                           taaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTTGTTTTCATGTCAGCTCCGGAGTAACGTAATTAAATAGCAACTCCCGCAAGAAGGAGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGtt                                                                                       <  2:570566/149‑1 (MQ=255)
                                                            aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGttt                                                                                      <  2:204349/149‑1 (MQ=255)
                                                            aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGttt                                                                                      >  1:1491379/1‑149 (MQ=255)
                                                             aaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTa                                                                                     >  2:454495/1‑149 (MQ=255)
                                                              aaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTaa                                                                                    <  1:1337536/149‑1 (MQ=255)
                                                                ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAtt                                                                                  <  1:1320236/149‑1 (MQ=255)
                                                                 gAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAttt                                                                                 <  1:1565792/149‑1 (MQ=255)
                                                                  aGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAtttt                                                                                <  1:735307/149‑1 (MQ=255)
                                                                  aGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAtttt                                                                                <  2:162784/149‑1 (MQ=255)
                                                                   gTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAttttt                                                                               <  2:389188/149‑1 (MQ=255)
                                                                    tAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTa                                                                              <  2:1114750/149‑1 (MQ=255)
                                                                      gggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaac                                                                            <  2:928886/149‑1 (MQ=255)
                                                                       ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaacc                                                                           >  1:650755/1‑149 (MQ=255)
                                                                        gAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaacca                                                                          >  1:612326/1‑149 (MQ=255)
                                                                          aTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaa                                                                        <  1:1029036/149‑1 (MQ=255)
                                                                           ttCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaac                                                                       <  2:1279349/149‑1 (MQ=255)
                                                                              aaGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAg                                                                    <  2:864164/149‑1 (MQ=255)
                                                                              aaGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACAAg                                                                    >  2:416174/1‑149 (MQ=255)
                                                                              aaGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGCCGTAAAGTAATTAAATAGCAACTCCCCCCAGAAGGCGGGAG‑TTTTTTACTGATTCTTAGTATATGGTGACGGAAATAAAACTTTCAAGTGTTTAATTTTTAAAAAAACCAg                                                                    >  1:1436394/1‑149 (MQ=255)
                                                                                       gTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTTCACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAAc                                                           <  1:935568/149‑1 (MQ=255)
                                                                                        ttAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGTCGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACa                                                          >  1:1447929/1‑149 (MQ=255)
                                                                                        ttAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACa                                                          >  2:284428/1‑149 (MQ=255)
                                                                                        ttAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAACGCGGGAG‑TTATTTACTGATGCTTAGTATATGTGGACGGAAAATACACTATCAAGTGTTTAATTTTTAACCAAACCAGTGATTGAACa                                                          >  2:571887/1‑149 (MQ=255)
                                                                                         tAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAt                                                         <  2:1491379/149‑1 (MQ=255)
                                                                                         tAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAt                                                         <  2:699786/149‑1 (MQ=255)
                                                                                             tttGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGCCGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaat                                                     >  2:414045/1‑149 (MQ=255)
                                                                                               tGGTTTTCATGTCAGCTCCTGCGTAACGTTTTTAAATAGCTACTCCCGACAGAAGTCGGGAG‑TTTTTTACTGATGCTTAGTATATGTGGACTGAAATTACACTTTCAAGTGTTTAATTTTTAACCTAACCAGTGATGGAACAttaattt                                                   <  1:571887/149‑1 (MQ=255)
                                                                                                  ttttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaat                                                <  2:519694/149‑1 (MQ=255)
                                                                                                  ttttCATGTCAGCTCCGGCGTAAAGTAATTTAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCATACCAGTGATGGATCAttaatttaat                                                >  2:578875/1‑149 (MQ=255)
                                                                                                   tttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATg                                               >  1:447513/1‑149 (MQ=255)
                                                                                                       aTGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAAt                                           >  2:1257250/1‑149 (MQ=255)
                                                                                                          tCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCaa                                        >  1:451227/1‑149 (MQ=255)
                                                                                                           cAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAAt                                       >  1:1234382/1‑149 (MQ=255)
                                                                                                               tCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAtt                                   >  1:1110476/1‑149 (MQ=255)
                                                                                                               tCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAtt                                   >  1:472999/1‑149 (MQ=255)
                                                                                                                 cGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAtttt                                 >  1:874496/1‑149 (MQ=255)
                                                                                                                   gCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGc                               <  1:401905/149‑1 (MQ=255)
                                                                                                                        acgtaaTTAAATCGCAACTCCCTCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaag                          <  2:273190/149‑1 (MQ=255)
                                                                                                                        acgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaag                          <  2:1482357/149‑1 (MQ=255)
                                                                                                                         cgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCGgaaga                         >  1:1467616/1‑149 (MQ=255)
                                                                                                                          gtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaagaa                        <  1:1385577/149‑1 (MQ=255)
                                                                                                                           taaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGaagaag                       <  1:843023/149‑1 (MQ=255)
                                                                                                                            aaTTTAAAATCAACTCCCGCCAGCATGCGGGTT‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGt                      <  1:1479369/149‑1 (MQ=255)
                                                                                                                             aTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGtt                     >  2:457733/1‑149 (MQ=255)
                                                                                                                               tAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTCATGCTTAGTATATGGGGACGGAAATTACACGTTCAAGTGTTTAATTTTTAACCAAACAAGTGATGGAACATAAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTAc                   >  2:816111/1‑149 (MQ=255)
                                                                                                                               tAAATAGCAACTCCCGCCAGAAGGAGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTAc                   <  1:1410053/149‑1 (MQ=255)
                                                                                                                                    aGCAACTCCCGCCAGATGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTcc              >  2:1400434/1‑149 (MQ=255)
                                                                                                                                      aaaCTCCCGCCTTCTGGCGGGAGTTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCg             >  2:789856/2‑149 (MQ=255)
                                                                                                                                      aaaCTCCCGCCTTCTGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCgg            >  2:1180973/2‑149 (MQ=255)
                                                                                                                                       aaCTCCCGCCTTCTGGCGGGAGTTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCgg            <  1:789856/149‑1 (MQ=255)
                                                                                                                                       aaCTCCCGCCTTCTGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCggg           >  2:664772/1‑149 (MQ=255)
                                                                                                                                       aaCTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCggg           >  2:1372652/1‑149 (MQ=255)
                                                                                                                                            ccgcccgcAGGCGGGAG‑TGTTTTACTGCTGCTTAGTATATGGGGACGGAAATTACACTTGCAAGTGTTTAAGTTTTACCCACCCCAGTGATGGAACATTAAGGTAATGTAAGCACTGATTTTGCCGAAGAAGTTCCCTGCCGGGATTTc      >  1:1172228/1‑149 (MQ=255)
                                                                                                                                            ccGCCAGACGGCGGGAG‑TTTTTTACTGAGGCTGAGTATATGGGGCCGGAAATTACACTTTCAAGTGGTTAATTTTTAACCAAACCAGTGAGGGCACATTAAGTTAATGTAATCAATGATTTTGCAGAAGCCTTTACCTTCCGGGAGTTc      >  1:1165923/1‑149 (MQ=255)
                                                                                                                                            ccGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAGATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGATACGTTCCGGGATTTc      >  2:241303/1‑149 (MQ=255)
                                                                                                                                            ccGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAttta      >  2:1464401/1‑148 (MQ=255)
                                                                                                                                             cGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGACGAAGAAGTTACCTTCCGGGATTTCt     >  1:116745/1‑149 (MQ=255)
                                                                                                                                              gCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCtt    <  1:215569/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGC  >  NC_000913/662538‑662830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑