Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 662,684 G→T 5.1% intergenic (‑42/+68) ybeD ← / ← dacA UPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913662,6840GT 5.1% 440.1 / 9.9 138intergenic (‑42/+68)ybeD/dacAUPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base G (72/59);  new base T (4/3);  total (76/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.94e-01

Only 100 of 141 total aligned reads displayed.

TACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAA  >  NC_000913/662538‑662832
                                                                                                                                                  |                                                                                                                                                     
     cTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCgg                                                                                                                                                >  2:215569/1‑149 (MQ=255)
      ttCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCggg                                                                                                                                               >  2:376068/1‑149 (MQ=255)
        cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAg                                                                                                                                             <  2:1458565/149‑1 (MQ=255)
        cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                                                                                                                             <  2:1238481/149‑1 (MQ=255)
         aaCCACCTGATCAACCAGCTCAGGTAACGCCTGCCACATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑t                                                                                                                                           >  2:58923/1‑149 (MQ=255)
          accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑tt                                                                                                                                          <  1:588042/149‑1 (MQ=255)
           ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑ttt                                                                                                                                         <  2:520899/149‑1 (MQ=255)
             accTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑ttttt                                                                                                                                       >  2:687450/1‑149 (MQ=255)
             accTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑ttttt                                                                                                                                       <  2:877454/149‑1 (MQ=255)
                tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTAc                                                                                                                                    >  2:935568/1‑149 (MQ=255)
                 gATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTAcg                                                                                                                                   >  1:908505/1‑148 (MQ=255)
                 gATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACt                                                                                                                                   >  2:735307/1‑149 (MQ=255)
                 gATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACt                                                                                                                                   >  2:465181/1‑149 (MQ=255)
                 gATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACt                                                                                                                                   >  1:162784/1‑149 (MQ=255)
                  aTCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTg                                                                                                                                  >  1:1279349/1‑149 (MQ=255)
                  aTCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTg                                                                                                                                  >  2:1029036/1‑149 (MQ=255)
                  aTCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTg                                                                                                                                  >  1:1379995/1‑149 (MQ=255)
                      aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTTCTGATGc                                                                                                                              >  1:247190/1‑149 (MQ=255)
                      aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGc                                                                                                                              >  2:183708/1‑149 (MQ=255)
                        taGCTAAGGTATGGGCTGCCCCATAACTTTGTAAGTCAAACGAGTAGGGAATTCAAGCATTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGATTAAATTAATTAAATATCAACTCCAGCCAGAAGGCGGGAG‑TTTTTTACTGATGCtt                                                                                                                            <  2:77311/148‑1 (MQ=255)
                          gCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAg                                                                                                                          <  2:607662/149‑1 (MQ=255)
                           cTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCTGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGt                                                                                                                         <  2:1388367/149‑1 (MQ=255)
                            tCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGta                                                                                                                        >  1:882293/1‑149 (MQ=255)
                                   aCGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAAAAGCAACTACCGCCAGCAGGAGGGAG‑TATTTTACTGATGCTTAGTATATgggg                                                                                                                 >  2:853715/1‑149 (MQ=255)
                                     gCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGAc                                                                                                               <  1:1250597/149‑1 (MQ=255)
                                       cTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACgg                                                                                                             >  2:766457/1‑149 (MQ=255)
                                           cccATAAATTTGTAAGTAAAATGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGAGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAAt                                                                                                         <  2:1232516/149‑1 (MQ=255)
                                            ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAAtt                                                                                                        >  1:703969/1‑149 (MQ=255)
                                            ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAAtt                                                                                                        >  1:875517/1‑149 (MQ=255)
                                             cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAAGTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                       <  2:880081/149‑1 (MQ=255)
                                             cATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                       >  2:1108516/1‑149 (MQ=255)
                                             cATAAATTTTTAAGTAAAAGTAGTAGGGAATTCAAGCAGTTCGTTCAGTTTGGTTTTCATGTCAGCTACGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCTGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                       <  2:1086559/149‑1 (MQ=255)
                                               tAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTaca                                                                                                     <  1:687450/149‑1 (MQ=255)
                                                   tttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACttt                                                                                                 <  2:393949/149‑1 (MQ=255)
                                                   tttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACttt                                                                                                 >  2:401905/1‑149 (MQ=255)
                                                   tttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACttt                                                                                                 <  1:1061255/149‑1 (MQ=255)
                                                    ttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTc                                                                                                <  2:1456151/149‑1 (MQ=255)
                                                     tGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCG‑TAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCaa                                                                                              <  1:996420/149‑1 (MQ=255)
                                                      gtaagtaaAAGGAGTCGGGAATTCAAGCAGTTCGTTAAGTCTGGTCTTCATGTCAGCTCCGGCGTAACGAATTTAATTAGCAACTCCCGCCAGAAGTCGTGAG‑TTTTTTACTGATGCTTACTATATGGGGACGGAAATTACACttgaaa                                                                                              <  1:248622/149‑5 (MQ=255)
                                                      gtaagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCaa                                                                                              >  2:824837/1‑149 (MQ=255)
                                                       taagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAg                                                                                             <  1:465181/149‑1 (MQ=255)
                                                            aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGttt                                                                                        >  1:1491379/1‑149 (MQ=255)
                                                               aGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGATTAGTATATGGGGACGGAAATTACACTTGCAAGTGTTTAAt                                                                                     >  1:118824/1‑149 (MQ=255)
                                                                ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAtt                                                                                    <  1:1320236/149‑1 (MQ=255)
                                                                  aGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAtttt                                                                                  <  2:162784/149‑1 (MQ=255)
                                                                    tAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTa                                                                                <  2:1114750/149‑1 (MQ=255)
                                                                      gggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaac                                                                              >  2:865375/1‑149 (MQ=255)
                                                                      gggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaac                                                                              <  2:928886/149‑1 (MQ=255)
                                                                       ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaacc                                                                             >  1:650755/1‑149 (MQ=255)
                                                                        gAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaacca                                                                            >  1:612326/1‑149 (MQ=255)
                                                                          aTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaa                                                                          <  1:1029036/149‑1 (MQ=255)
                                                                           ttCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaac                                                                         <  2:1279349/149‑1 (MQ=255)
                                                                              aaGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAg                                                                      <  2:864164/149‑1 (MQ=255)
                                                                              aaGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACAAg                                                                      >  2:416174/1‑149 (MQ=255)
                                                                              aaGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGCCGTAAAGTAATTAAATAGCAACTCCCCCCAGAAGGCGGGAG‑TTTTTTACTGATTCTTAGTATATGGTGACGGAAATAAAACTTTCAAGTGTTTAATTTTTAAAAAAACCAg                                                                      >  1:1436394/1‑149 (MQ=255)
                                                                                       gTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTTCACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAAc                                                             <  1:935568/149‑1 (MQ=255)
                                                                                       gTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAAAGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAAc                                                             >  2:565004/1‑149 (MQ=255)
                                                                                        ttAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGTCGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACa                                                            >  1:1447929/1‑149 (MQ=255)
                                                                                        ttAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACa                                                            >  2:284428/1‑149 (MQ=255)
                                                                                        ttAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAACGCGGGAG‑TTATTTACTGATGCTTAGTATATGTGGACGGAAAATACACTATCAAGTGTTTAATTTTTAACCAAACCAGTGATTGAACa                                                            >  2:571887/1‑149 (MQ=255)
                                                                                         tAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAt                                                           <  2:699786/149‑1 (MQ=255)
                                                                                               tGGTTTTCATGTCAGCTCCTGCGTAACGTTTTTAAATAGCTACTCCCGACAGAAGTCGGGAG‑TTTTTTACTGATGCTTAGTATATGTGGACTGAAATTACACTTTCAAGTGTTTAATTTTTAACCTAACCAGTGATGGAACAttaattt                                                     <  1:571887/149‑1 (MQ=255)
                                                                                                  ttttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaat                                                  <  2:519694/149‑1 (MQ=255)
                                                                                                  ttttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaat                                                  >  1:424461/1‑149 (MQ=255)
                                                                                                  ttttCATGTCAGCTCCGGCGTAAAGTAATTTAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCATACCAGTGATGGATCAttaatttaat                                                  >  2:578875/1‑149 (MQ=255)
                                                                                                   tttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATg                                                 >  1:447513/1‑149 (MQ=255)
                                                                                                       aTGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAAt                                             >  2:1257250/1‑149 (MQ=255)
                                                                                                          tCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCaa                                          >  1:451227/1‑149 (MQ=255)
                                                                                                           cAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAAt                                         >  1:1234382/1‑149 (MQ=255)
                                                                                                             gCTCAGTCGTAACGTAATTAAATAGCAACTCCCGCCAGAATGAGGGAG‑TTTTTTAATGATTCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGa                                       <  2:210865/149‑1 (MQ=255)
                                                                                                               tCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAtt                                     >  1:1110476/1‑149 (MQ=255)
                                                                                                                 cGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGTGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAtttt                                   <  2:247190/149‑1 (MQ=255)
                                                                                                                   gCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGc                                 <  1:401905/149‑1 (MQ=255)
                                                                                                                       aacgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaa                             <  2:401089/149‑1 (MQ=255)
                                                                                                                        acgtaaTTAAATCGCAACTCCCTCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaag                            <  2:273190/149‑1 (MQ=255)
                                                                                                                        acgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaag                            <  2:1482357/149‑1 (MQ=255)
                                                                                                                         cgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCGgaaga                           >  1:1467616/1‑149 (MQ=255)
                                                                                                                          gtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaagaa                          <  1:1385577/149‑1 (MQ=255)
                                                                                                                           taaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGaagaag                         >  1:653026/1‑149 (MQ=255)
                                                                                                                           taaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGaagaag                         <  1:843023/149‑1 (MQ=255)
                                                                                                                           taaTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGaagaag                         >  1:1460138/1‑149 (MQ=255)
                                                                                                                            aaTTTAAAATCAACTCCCGCCAGCATGCGGGTT‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGt                        <  1:1479369/149‑1 (MQ=255)
                                                                                                                             aTTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGtt                       >  2:457733/1‑149 (MQ=255)
                                                                                                                               tAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTAc                     <  2:655834/149‑1 (MQ=255)
                                                                                                                               tAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTCATGCTTAGTATATGGGGACGGAAATTACACGTTCAAGTGTTTAATTTTTAACCAAACAAGTGATGGAACATAAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTAc                     >  2:816111/1‑149 (MQ=255)
                                                                                                                               tAAATAGCAACTCCCGCCAGAAGGAGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTAc                     <  1:1410053/149‑1 (MQ=255)
                                                                                                                                  aTAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCtt                  <  1:183708/149‑1 (MQ=255)
                                                                                                                                    aaaaaCTCCCGCCTTCTGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTcc                >  2:1556402/4‑149 (MQ=255)
                                                                                                                                    aGCAACTCCCGCCAGATGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTcc                >  2:1400434/1‑149 (MQ=255)
                                                                                                                                      aaaCTCCCGCCTTCTGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCgg              >  2:1180973/2‑149 (MQ=255)
                                                                                                                                       aaCTCCCGCCTTCTGGCGGGAGTTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCgg              <  1:789856/149‑1 (MQ=255)
                                                                                                                                       aaCTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCggg             >  2:1372652/1‑149 (MQ=255)
                                                                                                                                            ccGCCAGACGGCGGGAG‑TTTTTTACTGAGGCTGAGTATATGGGGCCGGAAATTACACTTTCAAGTGGTTAATTTTTAACCAAACCAGTGAGGGCACATTAAGTTAATGTAATCAATGATTTTGCAGAAGCCTTTACCTTCCGGGAGTTc        >  1:1165923/1‑149 (MQ=255)
                                                                                                                                            ccGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAttta        >  2:1464401/1‑148 (MQ=255)
                                                                                                                                             cGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGACGAAGAAGTTACCTTCCGGGATTTCt       >  1:116745/1‑149 (MQ=255)
                                                                                                                                              gCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAGTCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCtt      <  1:871492/149‑1 (MQ=255)
                                                                                                                                              gCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCtt      <  1:215569/149‑1 (MQ=255)
                                                                                                                                               ccAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTg     <  2:451227/149‑1 (MQ=255)
                                                                                                                                                  gAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCaa  >  1:1563399/1‑149 (MQ=255)
                                                                                                                                                  gAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCaa  <  2:33681/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAG‑TTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAA  >  NC_000913/662538‑662832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑