Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 726,054 A→C 6.9% V328G (GTG→GGG)  kdpB ← potassium translocating ATPase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913726,0540AC 6.9% 182.7 / 4.4 69V328G (GTG→GGG) kdpBpotassium translocating ATPase, subunit B
Reads supporting (aligned to +/- strand):  ref base A (25/39);  new base C (2/3);  total (27/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

AACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCT  >  NC_000913/725907‑726199
                                                                                                                                                   |                                                                                                                                                 
accGGTACAAAGGTGGCATGGAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCGTGGCGAGGATCACAATACTGCGGCCGTCCGGCGTTGCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCAc                                                                                                                                                  <  2:1517475/147‑1 (MQ=255)
aaCGGTACAAAGGTGGCATGGAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCAc                                                                                                                                                  >  2:638197/1‑149 (MQ=255)
     tACAATGGTGGCATGGAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCCGCGTTTTTTCATCCCCGCCCt                                                                                                                                             >  1:75165/1‑149 (MQ=255)
     tACAAAGGTGGCATGGAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCt                                                                                                                                             >  1:925607/1‑149 (MQ=255)
           ggTGGCCTGGAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGGTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCgg                                                                                                                                       <  1:55180/149‑1 (MQ=255)
              ggCATGGAGCGACTGCACATCGCGCTCGCGCAGGTTATAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCCCGCGCAGGGCGTGGa                                                                                                                                    <  1:566635/149‑1 (MQ=255)
              ggCATGGAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGATTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCCCGCGCAGGGCGTGGa                                                                                                                                    <  1:1234858/149‑1 (MQ=255)
               gCATGGAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCGTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCCCGCGCAGGGCGTGGAt                                                                                                                                   <  2:799793/149‑1 (MQ=255)
                  tcgAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCCTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATaaa                                                                                                                                <  1:241406/147‑1 (MQ=255)
                     aGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTc                                                                                                                             <  2:1334879/149‑1 (MQ=255)
                      gCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTcc                                                                                                                            <  2:1347305/149‑1 (MQ=255)
                        gACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGa                                                                                                                          <  1:506271/149‑1 (MQ=255)
                         aCTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGAc                                                                                                                         >  1:576518/1‑149 (MQ=255)
                           tGCACAGCGCGCTCGCGCAGGCTACAACGCTGCGTGGCGAGGATCACACTACTGCGCCCTGCCGGCGTATCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGc                                                                                                                       <  1:104269/149‑1 (MQ=255)
                               caTCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACACCGACGCCTGa                                                                                                                   >  2:575065/1‑149 (MQ=255)
                                aTCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGAc                                                                                                                  >  1:353033/1‑149 (MQ=255)
                                aTCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTGTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGAc                                                                                                                  <  1:1057240/149‑1 (MQ=255)
                                 tcgcgctcgcgcAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACg                                                                                                                 >  1:67636/1‑149 (MQ=255)
                                      ctcgcgcAGGTTAAAACGCTGCTTGGCGAGGAGCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTAc                                                                                                            <  2:480588/149‑1 (MQ=255)
                                         gcgcAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGa                                                                                                         >  2:1520960/1‑149 (MQ=255)
                                                ttAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGAt                                                                                                  <  1:996317/149‑1 (MQ=255)
                                                ttAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGAt                                                                                                  <  1:806944/149‑1 (MQ=255)
                                                ttAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGAGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGAt                                                                                                  <  1:464058/149‑1 (MQ=255)
                                                 tAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATg                                                                                                 <  2:371781/149‑1 (MQ=255)
                                                     aCGCTGCTTGGCGAGGATCACAATACTGCGGGCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGc                                                                                             <  2:1267974/149‑1 (MQ=255)
                                                          gctTGGCTAGTATCACAATACTGCGTCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACTCCTGACGGTTACCGAGTGTGATGGTGCCGGtt                                                                                        <  2:1397846/149‑1 (MQ=255)
                                                            ttGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGtttt                                                                                      <  1:1073554/149‑1 (MQ=255)
                                                              ggCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTAt                                                                                    <  2:1516899/149‑1 (MQ=255)
                                                               gCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCCCCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTATCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATc                                                                                   <  1:299766/149‑1 (MQ=255)
                                                               gCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATc                                                                                   <  1:1483697/149‑1 (MQ=255)
                                                               gCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATc                                                                                   <  1:294963/149‑1 (MQ=255)
                                                               gCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATc                                                                                   <  2:1270171/149‑1 (MQ=255)
                                                                 gAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATccc                                                                                 >  1:1401900/1‑148 (MQ=255)
                                                                 gAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCa                                                                                 <  1:962595/149‑1 (MQ=255)
                                                                    gATCACAATACTGCTGCCTTCCGGCGTTTCATCAGGCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTATTTCATCCACGCCCTGCGAGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTa                                                                              <  1:298252/149‑1 (MQ=255)
                                                                    gATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTa                                                                              >  1:350114/1‑149 (MQ=255)
                                                                    gATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTa                                                                              <  2:577660/149‑1 (MQ=255)
                                                                           aTACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGaac                                                                       <  2:1460898/149‑1 (MQ=255)
                                                                           aTACTGCGGCCTTCCGGCGTATCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGaac                                                                       <  2:430571/149‑1 (MQ=255)
                                                                            tACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGaacg                                                                      <  2:390108/149‑1 (MQ=255)
                                                                                      ttCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCAc                                                            >  1:494188/1‑149 (MQ=255)
                                                                                      ttCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCG‑TTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACgtcaccgtcacc                                                           >  2:646242/1‑149 (MQ=255)
                                                                                          ggCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGc                                                        <  1:28419/149‑1 (MQ=255)
                                                                                             gTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGc                                                     <  2:350114/149‑1 (MQ=255)
                                                                                                tcatcaGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTc                                                  <  2:893122/149‑1 (MQ=255)
                                                                                                  atcaGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCaa                                                <  2:160479/149‑1 (MQ=255)
                                                                                                   tcaGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAAc                                               <  1:638197/149‑1 (MQ=255)
                                                                                                       ccagcGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCCCCTGCCGCTTCAACTGcc                                           >  1:813936/1‑148 (MQ=255)
                                                                                                          gcGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGt                                        >  1:1019990/1‑149 (MQ=255)
                                                                                                            gAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTcc                                      <  2:79943/149‑1 (MQ=255)
                                                                                                              cgCCAGTCGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGAGCCGGTTTAATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGc                                    <  1:1310696/148‑1 (MQ=255)
                                                                                                                   gTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCtggtg                               <  2:925607/149‑1 (MQ=255)
                                                                                                                   gTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCtggtg                               >  1:475155/1‑149 (MQ=255)
                                                                                                                   gTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCtggtg                               >  1:902855/1‑149 (MQ=255)
                                                                                                                       tgCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGGGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCCCCTGCCGCTTCAACTGCACGTCCGCTGGTGGCaa                           >  1:1439358/1‑149 (MQ=255)
                                                                                                                       agcggcgTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCaa                           >  1:582610/2‑149 (MQ=255)
                                                                                                                                 gccagcGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGGTTTATCCAGTAGCAGCACGTCAACGTCACCTGCCGCTTGCACTGCACGTCCGCTGGTGGCAATCACCTTcgc                 >  1:278994/1‑149 (MQ=255)
                                                                                                                                  ccagcGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTcgcg                >  2:1331956/1‑149 (MQ=255)
                                                                                                                                   cagcGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCgcgc               >  1:9680/1‑149 (MQ=255)
                                                                                                                                      cGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACCTTCGCGCCtt            >  1:1508733/1‑148 (MQ=255)
                                                                                                                                        ttttttCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGc          <  2:353033/149‑1 (MQ=255)
                                                                                                                                         tttttCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCa         <  2:538645/149‑1 (MQ=255)
                                                                                                                                         tttttCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCa         <  2:1019990/149‑1 (MQ=255)
                                                                                                                                           tttCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATc       >  2:787521/1‑149 (MQ=255)
                                                                                                                                           tttCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATc       >  2:965896/1‑149 (MQ=255)
                                                                                                                                            tttaTCCCCGCGCAGGGCGTGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATcc      >  2:1367035/4‑149 (MQ=255)
                                                                                                                                            ttCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATcc      >  2:54820/1‑149 (MQ=255)
                                                                                                                                              cATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCgg    <  1:732406/149‑1 (MQ=255)
                                                                                                                                                tCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGCCGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGCACGTCAACGTCACCTGCCGCTTCACCTGCCCGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCt  >  1:1434653/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AACGGTACAAAGGTGGCATGGAGCGACTGCACATCGCGCTCGCGCAGGTTAAAACGCTGCTTGGCGAGGATCACAATACTGCGGCCTTCCGGCGTTTCATCAGCCAGCGAAGCCAGTTGTGCGGCGTCAGCCAGCGTTTTTTCATCCACGCCCTGCGCGGGGATAAACTCCGACGCCTGACGGTTACCGAGTGTGATGGTGCCGGTTTTATCCAGTAGCAGAACGTCAACGTCACCTGCCGCTTCAACTGCACGTCCGCTGGTGGCAATCACATTCGCGCCTAGCATCCGGCT  >  NC_000913/725907‑726199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑