Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 774,648 A→T 5.2% intergenic (+46/‑104) ybgE → / → ybgC putative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913774,6480AT 5.2% 333.8 / 5.0 111intergenic (+46/‑104)ybgE/ybgCputative inner membrane protein in cydABX‑ybgE operon/acyl‑CoA thioester hydrolase
Reads supporting (aligned to +/- strand):  ref base A (55/50);  new base T (3/3);  total (58/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 114 total aligned reads displayed.

CGGCGTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTCTACTATGAA  >  NC_000913/774500‑774796
                                                                                                                                                    |                                                                                                                                                    
cGGCGTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATaaa                                                                                                                                                      >  1:1425074/1‑149 (MQ=255)
 ggCGTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTACATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAAt                                                                                                                                                     <  1:691809/149‑1 (MQ=255)
  gCGTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAAtt                                                                                                                                                    >  1:305557/1‑149 (MQ=255)
    gTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAAtttt                                                                                                                                                  <  2:699870/149‑1 (MQ=255)
    gTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAAtttt                                                                                                                                                  >  2:776993/1‑149 (MQ=255)
    gTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAAtttt                                                                                                                                                  <  2:1483772/149‑1 (MQ=255)
        gCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAATTTATGGGCCTGGAGCGGCCCATAATTTTTTaaa                                                                                                                                              <  2:867380/149‑3 (MQ=38)
        gCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAATTTATGGGCCTGGAGCGGCCCATAATTTTTTaaa                                                                                                                                              <  1:1596083/149‑3 (MQ=38)
          tttCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCt                                                                                                                                            >  1:405928/1‑149 (MQ=255)
          ctccgcccGCACAACGATCTTTGGCAACGGAATTTTTCCCCATTGCTCGCCGATCTTGTTCTCAATGACGGGCCGATTATCTACTTCATTTAAATCCGAATTCTCATTAAAAAATAACCGGCCGCCCCAGGCCCATAAATTTTTACGCt                                                                                                                                            <  2:131894/143‑1 (MQ=255)
               tCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCtt                                                                                                                                       >  1:605774/1‑149 (MQ=255)
                  gCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAAc                                                                                                                                    <  2:1440789/149‑1 (MQ=255)
                    aGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACtt                                                                                                                                  <  2:519990/149‑1 (MQ=255)
                      aaGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGc                                                                                                                                <  1:730078/149‑1 (MQ=255)
                        ggTTCTTTGGCAAGCGATATTTTGCCCAATGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAACTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGccc                                                                                                                              <  2:875314/149‑1 (MQ=255)
                         gTTCTTTGGCAAGGGTTTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCt                                                                                                                             <  1:664108/149‑1 (MQ=255)
                         gTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCt                                                                                                                             <  1:179116/149‑1 (MQ=255)
                                ggCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTccc                                                                                                                      <  2:861054/149‑1 (MQ=255)
                                 gCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATATTTACGCTCCCTTAACTTGCCATCATTCCCa                                                                                                                     >  1:387258/1‑149 (MQ=255)
                                    cgggagatttaGCCCCTTGCTTGCCGATATAGATCTCATTGTCGGGCGGATTTTCTTCTTCTTATTACTCCCAATTCTCTTTCAAAAATTATGGGCCGCCCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAAc                                                                                                                  <  2:285945/142‑1 (MQ=255)
                                    aGGGATTTTTTGCCCATTGCTTGCCGTTATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCCGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGTCCCATAAATTTTTACGCTCCGTTAACTTGACGTCATTCCCAAAc                                                                                                                  >  1:868000/1‑149 (MQ=255)
                                    aGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCaacc                                                                                                                  >  2:85909/1‑147 (MQ=255)
                                    aGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAAc                                                                                                                  <  1:311176/149‑1 (MQ=255)
                                    aGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAAc                                                                                                                  <  1:847072/149‑1 (MQ=255)
                                      ggATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTcc                                                                                                                       >  1:927325/1‑142 (MQ=255)
                                      ggATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTcc                                                                                                                       <  2:927325/142‑1 (MQ=255)
                                      ggATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCt                                                                                                                >  1:1114513/1‑149 (MQ=255)
                                       gATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTTAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTc                                                                                                               >  1:408836/1‑149 (MQ=255)
                                       gATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCACAAACCTc                                                                                                               >  2:714059/1‑149 (MQ=255)
                                         ttttttGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCATAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCaa                                                                                                             <  1:369473/149‑1 (MQ=255)
                                          tttttGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAAt                                                                                                            >  2:751444/1‑149 (MQ=255)
                                               gCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATcgcgc                                                                                                       <  2:1493673/149‑1 (MQ=255)
                                                cccATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATcgcgcg                                                                                                      >  1:116252/1‑149 (MQ=255)
                                                 ccATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATcgcgcgc                                                                                                     >  1:1305594/1‑149 (MQ=255)
                                                      gcttgcCTATATTGTTGTCATTTTCGGGCTTATTTTCTTCTTCTTTAAAATCAGAATTATCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGtata                                                                                                <  2:73843/149‑1 (MQ=255)
                                                       cttgcCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGTCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTAtag                                                                                               >  1:1034272/1‑149 (MQ=255)
                                                       cttgcCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTAtag                                                                                               <  2:305557/149‑1 (MQ=255)
                                                       cttgcCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTAtag                                                                                               <  1:1144629/149‑1 (MQ=255)
                                                        ttgcCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAAACGCGCGCGTAtagt                                                                                              >  1:803475/1‑149 (MQ=255)
                                                          gcCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTAtagtag                                                                                            <  2:488683/149‑1 (MQ=255)
                                                            cGATATTGTTCTCATTGTCTGGCTGATTTTCTTCTTCATTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCGTTAACTTGCCCTCATTCACAAACCTCAATCGCGCGCGTATAGTagca                                                                                          <  2:1108117/149‑1 (MQ=255)
                                                                atTGTTCTCATTGTAGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGt                                                                                      <  1:411444/149‑1 (MQ=255)
                                                                   gTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTa                                                                                   >  1:424385/1‑149 (MQ=255)
                                                                      ctcATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTaaaa                                                                                <  1:625597/149‑1 (MQ=255)
                                                                        cATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCGCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGc                                                                              >  2:700054/1‑149 (MQ=255)
                                                                         aTTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGct                                                                             >  2:197352/1‑149 (MQ=255)
                                                                         aTTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGct                                                                             >  2:209805/1‑149 (MQ=255)
                                                                         aTTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGct                                                                             <  2:1206775/149‑1 (MQ=255)
                                                                             tCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTaa                                                                         >  2:656829/1‑149 (MQ=255)
                                                                              cGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAAc                                                                        <  1:557547/149‑1 (MQ=255)
                                                                                ggCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCACAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACtt                                                                      >  1:1189734/1‑149 (MQ=255)
                                                                                 gCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATTAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTTTAGTAGCAGCGTTTAAAAGCTCTAACttt                                                                     >  1:225562/1‑149 (MQ=255)
                                                                                 gCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTACAGTCCCATAAATTTTTACGCTCCCTTAAATTGAACTAATTCACAAACCTCAATCGCGCGCTTATAGTAGAAGCGTTTAAAAGCTCTAACttt                                                                     >  1:77347/1‑149 (MQ=255)
                                                                                 gCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCATAAAAAAATTATGGGCAGCTCCAGGCCCATAAATATTTAAGCTCCCTTAACTTTCCCTCATTCACAAACATCAATCGAGCGCGAATAGAAGCAGCGGTTAAAAGCTCTAACttt                                                                     >  1:107355/1‑149 (MQ=255)
                                                                                 gCTGATTTTCTTCTTCTTTTAAATCAGAATTCACTTTAAAAAATTATGGGCCGCTCCAGGACAATAAATTTTTACTCTCCCTAAACTTGCCCTCATTCCCAAACCTCAATCGCGCGAGTATAGTAGCAGCGTTTAAAAGCTCTAACttt                                                                     >  1:146798/1‑149 (MQ=255)
                                                                                  cTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGATCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACtttt                                                                    >  2:582017/1‑149 (MQ=255)
                                                                                   tGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTttg                                                                   <  2:327729/149‑1 (MQ=255)
                                                                                    gATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAACTTATGGGCCGCTCCAGGCCCATAAATTTTTAGGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTttgt                                                                  <  2:295081/149‑1 (MQ=255)
                                                                                     aTTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTttgtt                                                                 >  2:1323215/1‑149 (MQ=255)
                                                                                     aTTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTttgtt                                                                 <  1:622152/149‑1 (MQ=255)
                                                                                         tcttcttctTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCAt                                                             <  1:775974/149‑1 (MQ=255)
                                                                                          cttcttcttTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTACCAAACCTCAATCGCGCGCGTATATTAGCAGCGATAAAAAGCTCTAACTTTTCTTGCAtt                                                            >  1:1317783/1‑149 (MQ=255)
                                                                                            tcttcttTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTAc                                                          >  2:684120/1‑149 (MQ=255)
                                                                                            tcttcttTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTAc                                                          >  1:129617/1‑149 (MQ=255)
                                                                                              ttcttTTAAATCATAATTATCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTTCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCg                                                        <  2:701998/149‑1 (MQ=255)
                                                                                              ttcttTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCg                                                        <  2:116252/149‑1 (MQ=255)
                                                                                               tcttTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCgg                                                       >  2:6630/1‑149 (MQ=255)
                                                                                               tcttTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCgg                                                       <  2:1562899/149‑1 (MQ=255)
                                                                                                 ttttAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAATTTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGa                                                     >  1:380495/1‑149 (MQ=255)
                                                                                                 ttttAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGa                                                     <  2:844878/149‑1 (MQ=255)
                                                                                                 ttttAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGa                                                     >  2:964735/1‑149 (MQ=255)
                                                                                                 ttttAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTAAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGa                                                     >  2:970015/1‑149 (MQ=255)
                                                                                                      aaTCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTttg                                                                   >  2:1597060/1‑130 (MQ=255)
                                                                                                      aaTCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTttg                                                                   <  1:1597060/130‑1 (MQ=255)
                                                                                                      aaTCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTaa                                                >  2:384036/1‑149 (MQ=255)
                                                                                                       aTCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTaaa                                               >  2:657088/1‑149 (MQ=255)
                                                                                                            aaTTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGAt                                                    <  2:1211877/139‑1 (MQ=255)
                                                                                                                 tctTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAACCCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACaa                                     >  2:773519/1‑149 (MQ=255)
                                                                                                                     tAAAAATTTATGGGCCTGGAGCGGCCCATAATTTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACATCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGATCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCt                                 >  2:33891/1‑149 (MQ=38)
                                                                                                                     tAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCt                                 <  1:885480/149‑1 (MQ=255)
                                                                                                                     tAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTACCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCt                                 <  1:147199/149‑1 (MQ=255)
                                                                                                                       aaaaaTTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACACCGCTGt                               >  2:1039479/1‑149 (MQ=255)
                                                                                                                       aaaaaTTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGt                               >  2:1513078/1‑149 (MQ=255)
                                                                                                                          aaTTATGGGCCGCTCCAGGCCCATTAATTTTTACGCTCCCTTAACTTGACCTCATTCCCGAACCTCAATCGCGCGGGTATAGTAGCAGCGTTTCAAAGCTGTAACTTTTGTTGCTTTCACGGGATGTAAAGTGATTACAACGCTGTTTc                            >  1:100919/1‑149 (MQ=255)
                                                                                                                          aaTTATGGGCCGCTCCAGGCCCATAATTTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACATCAATCGCGCGCGGATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCTTTACCGGGATGTAAAGTGAATACAACGCTGTTTc                            >  1:1024265/1‑149 (MQ=255)
                                                                                                                            ttATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGa                          <  2:1425074/149‑1 (MQ=255)
                                                                                                                             tATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGAt                         <  1:751444/149‑1 (MQ=255)
                                                                                                                                   ccGCTCCATGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCgg                   <  2:146798/149‑1 (MQ=255)
                                                                                                                                   ccGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCgg                   <  2:225562/149‑1 (MQ=255)
                                                                                                                                       tCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTCTTTCGATGGCCGGTTcg               >  1:289618/1‑149 (MQ=255)
                                                                                                                                        ccAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTcgc              <  1:656829/149‑1 (MQ=255)
                                                                                                                                         cAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTAGAGTAGCAGAGTTTAAAAGCTCTAACTATTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTAGATGGCCTGTTagcg             >  2:1364250/1‑149 (MQ=255)
                                                                                                                                          aGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGt            >  1:781819/1‑149 (MQ=255)
                                                                                                                                             cccATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTcta         <  1:1513078/149‑1 (MQ=255)
                                                                                                                                              ccttAAATTTTTACGCTCCCTTCACTTGCCCTCATTCAAAAACCTCAATCGAGAGCGTATAGTATGATCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTctac        <  2:314265/146‑1 (MQ=255)
                                                                                                                                              ccATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTctac        >  2:732937/1‑149 (MQ=255)
                                                                                                                                              ccATAAATTTTTACGCTCACTTAACTTGACCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTctac        <  2:544731/149‑1 (MQ=255)
                                                                                                                                                 tAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTCTACTAt     >  2:1262946/1‑149 (MQ=255)
                                                                                                                                                  aaaTTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTCTACTATg    <  2:1034272/149‑1 (MQ=255)
                                                                                                                                                    aTTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTCACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTCTACTATGaa  <  1:776993/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGCGTGGGCTTTCGTCCGCAGAAGGTTCTTTGGCAAGGGATTTTTTGCCCATTGCTTGCCGATATTGTTCTCATTGTCGGGCTGATTTTCTTCTTCTTTTAAATCAGAATTCTCTTTAAAAAATTATGGGCCGCTCCAGGCCCATAAATTTTTACGCTCCCTTAACTTGCCCTCATTCCCAAACCTCAATCGCGCGCGTATAGTAGCAGCGTTTAAAAGCTCTAACTTTTGTTGCATTACCGGGATGTAAAGTGAATACAACGCTGTTTCGATGGCCGGTTCGCGTCTACTATGAA  >  NC_000913/774500‑774796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: