Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 850,282 C→A 8.4% intergenic (‑185/‑168) rhtA ← / → ompX threonine and homoserine efflux system/outer membrane protein X

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913850,2820CA 8.4% 395.0 / 18.1 136intergenic (‑185/‑168)rhtA/ompXthreonine and homoserine efflux system/outer membrane protein X
Reads supporting (aligned to +/- strand):  ref base C (64/60);  new base A (7/5);  total (71/65)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 136 total aligned reads displayed.

GAGTGTACTGGATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTT  >  NC_000913/850136‑850429
                                                                                                                                                  |                                                                                                                                                   
gAGTGTACTGGATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACtt                                                                                                                                                   <  1:1113873/149‑1 (MQ=255)
   tgtACTGGATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACg                                                                                                                                                >  1:634684/1‑149 (MQ=255)
     tACTGGATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCa                                                                                                                                              >  1:537070/1‑149 (MQ=255)
     tACTGGATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAAGTATTACGCa                                                                                                                                              >  2:1371992/1‑149 (MQ=255)
       cTGGATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGTAAAACATGCGTAATACTTACGCAGt                                                                                                                                            >  2:191666/1‑149 (MQ=255)
        tGGATATACCCGATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGtt                                                                                                                                           <  2:674615/149‑1 (MQ=255)
        tGGATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGtt                                                                                                                                           >  1:1267520/1‑149 (MQ=255)
        tGGATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAAGTATTACGCatgt                                                                                                                                           <  2:413202/149‑4 (MQ=255)
          gATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACATGGAAAACATGCGTAATACTTACGCAGTtct                                                                                                                                         >  1:298737/1‑149 (MQ=255)
               aCCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGAAAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGaa                                                                                                                                    >  1:209822/1‑149 (MQ=255)
                cccAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGaaa                                                                                                                                   >  1:1479747/1‑149 (MQ=255)
                 cctaTGCTGGTTGATCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCGAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGaaaa                                                                                                                                  <  2:636245/146‑1 (MQ=255)
                 ccAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGaaaa                                                                                                                                  >  2:905079/1‑149 (MQ=255)
                 ccAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGaaaa                                                                                                                                  >  2:753967/1‑149 (MQ=255)
                  cAATGCTGGTTTAGCATTTGTTGAACAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGTATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGaaaaa                                                                                                                                 <  2:1080945/149‑1 (MQ=255)
                  cAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCAGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGaaaaa                                                                                                                                 <  2:469058/149‑1 (MQ=255)
                   aaTGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGaaaatg                                                                                                                                >  1:542267/1‑147 (MQ=255)
                   aaTGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAg                                                                                                                                <  1:1407629/149‑1 (MQ=255)
                   aaTGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAg                                                                                                                                <  1:1441669/149‑1 (MQ=255)
                      gCTGGTTGAGCATTTGTTGAAAAAATTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGAt                                                                                                                            <  2:14367/149‑1 (MQ=255)
                            tGAGCATTTGTTTAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTaaa                                                                                                                       <  1:1566128/149‑1 (MQ=255)
                            tGAGCATTTGTTGAAAAAATTTTCCCCCTTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTaaa                                                                                                                       <  1:1020839/149‑1 (MQ=255)
                               gCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAAttt                                                                                                                    >  1:661573/1‑149 (MQ=255)
                                cgtttGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTa                                                                                                                   <  1:141030/147‑1 (MQ=255)
                                cATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTa                                                                                                                   <  2:1148543/149‑1 (MQ=255)
                                     gttgAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATgg                                                                                                              >  1:628377/1‑149 (MQ=255)
                                      ttgAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGa                                                                                                             >  1:616049/1‑149 (MQ=255)
                                      ttgAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGa                                                                                                             >  1:979964/1‑149 (MQ=255)
                                       tgAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGAt                                                                                                            >  1:871243/1‑149 (MQ=255)
                                        gAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATa                                                                                                           >  2:1057390/1‑149 (MQ=255)
                                         aaaaaaTTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAg                                                                                                          >  2:1478506/1‑149 (MQ=255)
                                         aaaaaaTTTTCCAACTCTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGTGAAAACATGCGTAATACTTACTCATTTCTCTTAAAAAGTGATTTAAATTTAGATGGATAg                                                                                                          <  1:1229787/149‑1 (MQ=255)
                                             aaTTTTCCCCCGTTTTGACTAAGATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGgt                                                                                                      <  2:510279/149‑1 (MQ=255)
                                             aaTTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGgt                                                                                                      >  1:827379/1‑149 (MQ=255)
                                                  tCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAAGTATTACGCatgt                                                                                                                                           >  1:30011/1‑104 (MQ=255)
                                                  tCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAAGTATTACGCatgt                                                                                                                                           <  2:30011/107‑4 (MQ=255)
                                                     cccGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGt                                                                                                                               <  1:1385554/116‑1 (MQ=255)
                                                      ccGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGaaa                                                                                             <  1:814113/149‑1 (MQ=255)
                                                           ttGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTc                                                                                        >  1:1155615/1‑149 (MQ=255)
                                                            tGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAATAGATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCt                                                                                       <  1:178500/149‑1 (MQ=255)
                                                              aCTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGt                                                                                     <  1:194381/149‑1 (MQ=255)
                                                                tAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTa                                                                                   >  2:591115/1‑149 (MQ=255)
                                                                tAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTAGGGAAACGTTCTGTTa                                                                                   >  2:908176/1‑149 (MQ=255)
                                                                 aaaaTGCGCCAGGATTGATGGAATCATTAGGCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTAc                                                                                  <  1:1454382/149‑1 (MQ=255)
                                                                   aaTGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACAt                                                                                <  1:810019/149‑1 (MQ=255)
                                                                   aaTGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACAt                                                                                <  1:1487942/149‑1 (MQ=255)
                                                                       cgcCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGaaa                                                                            >  2:532533/1‑149 (MQ=255)
                                                                        gcCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAAt                                                                           <  2:661573/149‑1 (MQ=255)
                                                                           aGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGc                                                                        <  2:196675/149‑1 (MQ=255)
                                                                             gATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGccc                                                                      >  2:203200/1‑149 (MQ=255)
                                                                               ttGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGt                                                                    <  2:1322504/149‑1 (MQ=255)
                                                                                 gATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTa                                                                  <  2:911161/149‑1 (MQ=255)
                                                                                 gATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTa                                                                  <  2:1238557/149‑1 (MQ=255)
                                                                                 gATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGAGGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTa                                                                  <  2:1255611/149‑1 (MQ=255)
                                                                                  aTGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTa                                                                                                                                                  >  2:858799/1‑68 (MQ=255)
                                                                                  aTGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTa                                                                                                                                                  <  1:858799/68‑1 (MQ=255)
                                                                                   tGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGGTCTCTGAAAAAGTGATTTAAATTTAGATGGATATCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGa                                                                >  2:805740/1‑149 (MQ=255)
                                                                                    ggAATCATTAGTATGGTGATTAGGTATAATCTGTATGAATGACAGGGAAAACATGCGTAATACTTAAGCAGTTGTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGAc                                                               <  2:786310/149‑1 (MQ=255)
                                                                                     gAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACAAGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGTAGGATGGAAACGTTCTGTTACATGAAATGGCCCGAAAGACa                                                              >  2:715357/1‑149 (MQ=255)
                                                                                      aaTCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACAt                                                             <  2:1562767/149‑1 (MQ=255)
                                                                                              gTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATc                                                     <  1:1427280/149‑1 (MQ=255)
                                                                                              gTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATc                                                     >  2:972880/1‑149 (MQ=255)
                                                                                               tCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATcg                                                    <  2:628377/149‑1 (MQ=255)
                                                                                                cTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACTCAGTTCTCAGATAAAGTGATTTAAATTTAGATGGATAGCGGTGAATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACAACACCAATcgc                                                   >  2:254318/1‑149 (MQ=255)
                                                                                                cTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATcgc                                                   <  2:820460/149‑1 (MQ=255)
                                                                                                  ggTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGAGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGAGAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGa                                                 <  2:979964/149‑1 (MQ=255)
                                                                                                   gTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGaa                                                <  1:773122/149‑1 (MQ=255)
                                                                                                        tAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGtt                                           <  2:634684/149‑1 (MQ=255)
                                                                                                          gtaataatCTGGATGAATTACAGGGAAAACATTCTAAAAAATTTCGCAGATCTCTGAAAAAGTGATTTAAATATAGATGGATAGATTTGAATGTAAACGTTCTGTTAAATGAAATTGCCCGTTAGACAACACAAAACGCGAAGAGTTTc                                         >  2:1139661/3‑149 (MQ=255)
                                                                                                           gAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTcc                                        <  2:179248/149‑1 (MQ=255)
                                                                                                                 tCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTaa                                  >  2:1480211/1‑149 (MQ=255)
                                                                                                                 tCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTaa                                  >  2:1325242/1‑149 (MQ=255)
                                                                                                                     gATTAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTTATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAAtttt                              <  1:364565/149‑1 (MQ=255)
                                                                                                                     gATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAAtttt                              >  2:351952/1‑149 (MQ=255)
                                                                                                                        tatagaCAGTGAAAACATGCGTAATACTTACGCATTTCTCTGAAAAATTGATTTAAATTTCGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGa                           <  1:123256/145‑1 (MQ=255)
                                                                                                                        taatgaCAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGa                           <  1:1478506/148‑1 (MQ=255)
                                                                                                                        gaatgaCAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGa                           <  1:905079/149‑1 (MQ=255)
                                                                                                                        gaatgaCAGGGAAAACATGCGAAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCACATTAATTTTTGa                           >  1:30928/1‑149 (MQ=255)
                                                                                                                            gaCAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGatata                       >  1:1042068/1‑149 (MQ=255)
                                                                                                                             aCAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGatatat                      >  2:582962/1‑149 (MQ=255)
                                                                                                                              cAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATAtt                     <  2:581355/149‑1 (MQ=255)
                                                                                                                              cAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATAtt                     >  2:860584/1‑149 (MQ=255)
                                                                                                                               aGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATAttt                    >  1:549297/1‑149 (MQ=255)
                                                                                                                                gggAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTa                   >  1:283055/1‑149 (MQ=255)
                                                                                                                                  agagaactGCGTAAGTATTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTaaa                 >  1:1167523/8‑149 (MQ=255)
                                                                                                                                   aaaaCATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGTGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTaaaa                <  1:60610/149‑1 (MQ=255)
                                                                                                                                    agaactGCGTAAGTATTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGa                           <  2:1433841/132‑1 (MQ=255)
                                                                                                                                    agaactGCGTAAGTATTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGa                           >  1:1392080/6‑137 (MQ=255)
                                                                                                                                    agaactGCGTAAGTATTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGa                           >  1:1433841/6‑137 (MQ=255)
                                                                                                                                    agaactGCGTAAGTATTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGa                           <  2:1392080/132‑1 (MQ=255)
                                                                                                                                    aaaCATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAAc               >  1:1049254/1‑149 (MQ=255)
                                                                                                                                        aTGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAg           >  1:229344/1‑149 (MQ=255)
                                                                                                                                        aTGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAg           >  1:1318105/1‑149 (MQ=255)
                                                                                                                                        aTGCGTAATAATTACGCAGTTCTCTGAAAATGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGTAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAg           <  2:209822/149‑1 (MQ=255)
                                                                                                                                         tGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAgg          >  2:695391/1‑149 (MQ=255)
                                                                                                                                           ggTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTTTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGAc        <  2:542267/148‑1 (MQ=255)
                                                                                                                                             tAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACtt      >  2:859195/1‑149 (MQ=255)
                                                                                                                                             tAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTAAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACtt      >  1:320122/1‑149 (MQ=255)
                                                                                                                                                 aCTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATTGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTAttt  <  1:1268653/149‑1 (MQ=255)
                                                                                                                                                 aCTTACGCAGTTCTCTGAAAAAGTGATTTAAAGTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTAttt  <  1:1102600/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GAGTGTACTGGATATACCCAATGCTGGTTGAGCATTTGTTGAAAAAATTTTCCCCCGTTTTGACTAAAATGCGCCAGGATTGATGGAATCATTAGTCTGGTGATTAGGAATAATCTGGATGAATGACAGGGAAAACATGCGTAATACTTACGCAGTTCTCTGAAAAAGTGATTTAAATTTAGATGGATAGCGGTGTATGGAAACGTTCTGTTACATGAAATGGCCCGTTAGACATCACAAATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTT  >  NC_000913/850136‑850429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: