Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,708,844 | 0 | C | G | 41.2% | ‑2.5 / 10.6 | 18 | A642A (GCC→GCG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
Reads supporting (aligned to +/- strand): ref base C (8/2); new base G (4/3); total (13/5) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.93e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
GCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTG > NC_000913/1708695‑1708992 | gcagCTATTGCACGTGCCAAAGCGCGCAATCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc < 1:584561/147‑1 (MQ=255) aTTCCCCGGGCCCAACCGCGCAAGCTGGCACACCAACAGCCTACTCCGGAAGCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa < 1:896174/150‑1 (MQ=11) ggatAATTCCGAAGCAGAACCACATGTTGATCCTCGCAAACCTGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGc < 1:1438788/147‑1 (MQ=11) cTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAGCCCTAAAACACAAAAAGCAACAGGCTCATGAAGGGCAAGAAGAAAAAGAGGATAAGCGCAAAGCGGAAGGCGAAGCCg > 2:1847840/1‑150 (MQ=2) cAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCAAAAGCCCAAAATCACGCACAGCAAAAGGATAATTACGTAACAGCAGAACAGGTGGATACACTCAAAACAGCAGTAGAAGCCGCTATTGCCCGAGCCaaatca > 2:278554/1‑147 (MQ=0) gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCGGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGACGACGCGGCTATTGCCCGCGCCCAGGCCCACaaa > 1:15357/1‑150 (MQ=14) aaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCGGGAACAGc > 1:1515791/1‑150 (MQ=255) cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTAGATCCGCGCAACGCCGCCCTCGAAGCCGCGATTGCCCGGGCCATAGCGAGCAACCGGGACCCGCAACAGGCTaa > 1:1966346/1‑150 (MQ=11) tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCCGTAGCAGCCGCTATTGCCCGCGCCCAGGCCAAAAACCCCGCACAGCAACCGCCTAATGCAGAGCCaaa > 1:707662/1‑148 (MQ=14) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaag > 1:1451200/1‑150 (MQ=255) aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTTGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGCGCACACGCCCCAAAACACGCACAGCAACAGGATAAAACGGAGCCCGAAAAActgtta > 1:76928/1‑145 (MQ=9) gCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCCAAGCGAGCAAACCCGAACAGCAGCCGGGTAATCAGGAGCCAGAAGAACAGGTTGATAcgc > 2:781409/1‑150 (MQ=14) tGGAACAGCAACAGGCTAATGCGGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAc < 2:95927/102‑1 (MQ=255) tGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAc > 1:95927/1‑102 (MQ=12) cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGa < 2:1565146/92‑1 (MQ=16) cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGa > 1:1565146/1‑92 (MQ=16) cTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAACCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTAGAACCGCGCAAAGCTGCCGTCGACGCgg > 1:165543/1‑150 (MQ=255) tGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGa < 1:349682/81‑1 (MQ=255) tGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGa > 2:349682/1‑81 (MQ=255) ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCCCCCAAAGGCGACCAGCAACCGGGTAATGAGGAGCAATAAGAACAGGTAGCTCAGCCCAAATCGGCTGTAGCAGCGGCAATTg > 2:366102/1‑150 (MQ=9) ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCCAAGCCAACAAACGGGAACAGCAACATGCTAATACGGAGCCAGAAGAACAGGTAGATCCGCGCCAAGCTGCCGTCGACGCGGCTATTg > 2:1002644/1‑150 (MQ=14) | GCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTG > NC_000913/1708695‑1708992 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |