| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,269,994 | 0 | G | T | 36.4% | 42.7 / 13.1 | 56 | intergenic (+392/+222) | yhaC/rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Reads supporting (aligned to +/- strand): ref base G (12/23); new base T (0/20); total (12/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACC > NC_000913/3269845‑3270116 | gaatcgcgcctGAACGGTTTATTACGTACATCATGTAAAACAGACCGTTAAGACGCTTTCTTTTGGGTATAGTGGCGTGGACAGTCATTCATCTTTCTGCCGCTCCTAAAGTAAAAACCCGCAGAATCGGGTTTTTACGTAAAACAGGTg < 2:883195/144‑1 (MQ=17) cccGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAc < 1:640744/150‑1 (MQ=255) cgtaaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc < 1:1312794/147‑1 (MQ=255) cgGAATTGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc < 2:1793477/150‑1 (MQ=255) cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc < 1:1016836/150‑1 (MQ=255) cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc < 1:1972342/150‑1 (MQ=255) ggAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCATTCTGGCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGGTTTTAAGTAAAACAGGTGTAAcggcacg > 1:903871/1‑144 (MQ=14) gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa < 1:1002057/150‑1 (MQ=255) gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGAATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa < 1:1002291/150‑1 (MQ=255) gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTATTGGGTATAGAGTCGTGGACAATCATTAATAATTATTCCCCTCCAAAACAAAAAACCCACCGAAGCGGGGTTTTACGGAAATCAGGTGAAACTGCCCgc > 2:1920248/1‑149 (MQ=14) tcGGTTTATTACGTACATCAGGTAAAACTTACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATa < 2:1444904/149‑1 (MQ=255) gTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCACATACTTTTGGGTATAGAGTCTTGTAAAGTCAATCAAAATTATGACCCTCAAAAAGAAAAAAACAACAGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCAAttcgg > 2:1105723/1‑146 (MQ=9) gTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGATAAGCCGctttctt < 2:642891/150‑1 (MQ=255) taaaTCAGGTAAAAGTGACAGATAAGCAGCATTCATTTTTGTATTGTGTCGTGGACAGTGTTTCTTATTACTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGctttcttt < 2:972613/147‑1 (MQ=11) atcatttAATACTTAACGATAAGCCGCTTTCTTTTGGGTATAGTGTCTTCGACAGTCATTCATCTTTCTGCCCCTCCAATAGTATAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg < 2:583871/144‑1 (MQ=11) tgaGGTAATACTGAGCGATAAGCTGCTTGCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGTAAAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg < 1:540342/148‑1 (MQ=11) tCATGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCTTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg < 1:596520/150‑1 (MQ=255) acatgTAATACTGACCGATAAGCCGCTTTCTTTTTTCTATAGTGTCGTGGAAAGTCTTTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTggg < 1:495866/146‑1 (MQ=11) atgTAAAACTGACCGATAAGCCGCTTTCTTTTGCGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGta < 1:1955890/148‑1 (MQ=37) aGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAACTTTCAGCCCCTCCAAAAGAAAAAACCCACCGCATCGGGGTTGTTAGTATAAAAGGTGTTACTGAACGTAATGGCGCTTTCTTTTGTGta > 2:1527784/1‑150 (MQ=2) aCTGACCGATAATCCGCATTCTTTTGGGGATAGTGTCGTCGAAAGTCATTCATCTTTCTGCCCCTCCAAAAGTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTc < 1:435900/150‑1 (MQ=11) tGACCGATAAGTTGCTTTCATTTGTTTATTTTGTCGTGGACAGTGTTTCATATTTCTGCCACTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGt < 1:588727/150‑1 (MQ=11) tGACCGATAAGCCGCTTTCTTTTGGGTATAGTCTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGt < 1:1659970/150‑1 (MQ=37) cGATAAGCCGCAGGCTTTAGGATTATGTGTCGTGGAAAGTCTTTCTTCTTTCTGCCCCTCAAAAAGTAAAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAc < 1:1238642/150‑1 (MQ=11) ttatgCCGCTTTCTTTTTGGTTTAGTGTCGTGGACAGTCATTCATCTTTATGCCACTCAAAAATTATAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGGTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAg < 1:985809/146‑1 (MQ=11) agacGCTTTCTTTTGTTTAATGTGTCGTCGAAAGTCATTCATCTTTATGCCCCTCCAAAATTATAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtca < 1:1610817/147‑1 (MQ=11) gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAc > 1:949192/1‑103 (MQ=17) gCTTTCTTTTGTGGATCGTGTCGTGGACTGGCATTCTTCTTTCTGCCCCTCCAAAAGGAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattca < 2:1646601/150‑1 (MQ=11) ttttGTGTTTAGTGTCGTGGACAGTCATTAATCTATATGCCCCTCAACAAGTAAAAACCCGACGTATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTc < 2:1812784/150‑1 (MQ=11) tGGTTATAGTGTCGTGGACTGTGTTTCATCTTTCTGGCACTCCAAAATTAAAAACCCGCCGTATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGc < 2:1709416/150‑1 (MQ=11) tgctaGTGTCGTCGACAGTGTATCTTATATATGCCACTCCAAAAGAAAAAACCCGCCGTAGCGGGTTTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGcccc < 1:1754892/147‑1 (MQ=11) ggtttgtgtCGTCGACAGTCAATCTTATTTCTGCCCCTCCAAAAGTATAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGcccc < 1:1447532/145‑1 (MQ=11) gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCgg > 2:1790962/1‑92 (MQ=17) atatatGCCACTCAAAAATCACAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCACTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg < 1:1278735/145‑1 (MQ=11) ttatcgcccTCAAAAAGTACAAACCCGCCGAATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg‑gg < 1:1328099/144‑1 (MQ=11) tctcgcccTCCAAAATAACAAACCCGCCGTAGCGGGTTATTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGt < 1:1990256/145‑1 (MQ=11) gcacTCAAAAAGTATAAACCCGCCGTATCGGGTTTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGttttt < 2:940398/147‑1 (MQ=11) cTCCAAAATTAAAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCG‑GGtttttgct < 2:1924392/150‑4 (MQ=11) cTCCAAAAGTATATAACCGCCGATGCGGGTTTTTACGTAAATCAGGTGAAACTGTCGGATAATCCTCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACg < 1:1837330/150‑1 (MQ=11) cTCAAAAATTATAAACCCGCCGTTTCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACg < 2:6916/150‑1 (MQ=11) tcaaaaaGTAAAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCG‑GGtttttgctt < 2:1665109/147‑5 (MQ=11) ccAAAATCACAAACCCGCCGAACCGGTTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTa < 1:602045/150‑1 (MQ=11) ccAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGc > 2:1504483/1‑65 (MQ=255) cAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTCTTCCCCTCCAAAACCAAAAACCCGCCGGAACG‑GGAATTTACTTaa > 2:1912503/1‑150 (MQ=11) atctcaaaCCCGCCGTATCGGTTTTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTAAACCa < 2:1160716/145‑1 (MQ=11) gTAAAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg > 1:1361857/1‑114 (MQ=11) gTAAAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg < 2:1361857/114‑1 (MQ=11) acaaaCCCGCAGCAACGGTTTTTTACGTAAAACAGGTCAAACTGACCTATAAGCCGCTTTCATTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCCCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTAAACCAGGt < 1:122822/148‑1 (MQ=11) aaaaaCCCGCCGTATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTAAACCAGGt < 2:1598867/150‑1 (MQ=255) aaaaCCCGCCGTATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTAAACCAGGTg < 2:1821410/150‑1 (MQ=255) ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGTCTCGTGGAGAGTCATTCATCGTTCATCCCCAACAAAAACAAAAACACGCAGAAGGGCGGTTTTAAGGTAAA‑CACGTGaaaaa > 1:1074683/1‑148 (MQ=2) cgtaGCGGTTTATTACGTATAACTGTTTAAACTGTGCGATATGCCGCTTTCTTTTGTTTTTTGTGTCGTGGACTGTCTTTCTTCTTTCTGCCCCTCCAAAAGCATAAACCCGCCGTAGCG‑GGTTTTTACGTAAATCAGGTGAAACTGAcc < 2:1623032/147‑1 (MQ=2) cgtaGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGAcc < 1:1700741/147‑1 (MQ=255) cgtaGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGAcc < 1:1511851/147‑1 (MQ=255) cggatcGGTTTTTTACGTATATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGAcc < 1:192673/145‑1 (MQ=11) gggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGtcattcat > 2:870568/1‑77 (MQ=21) cGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc > 1:344203/1‑99 (MQ=37) | GAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCG‑GGTTTTTACGTAAACCAGGTGAAACTGACC > NC_000913/3269845‑3270116 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |