Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,473,927 C→A A43S (GCC→TCC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,473,9270CA98.5% 452.7 / ‑5.7 135A43S (GCC→TCC) bdcAc‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (64/69);  total (65/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473783‑4474073
                                                                                                                                                |                                                                                                                                                  
ccTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCtt                                                                                                                                               >  2:981748/1‑150 (MQ=255)
           aCGCCAATACCTGCATTTACCACCAGTATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCa                                                                                                                                    <  2:1272570/150‑1 (MQ=255)
            cGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCGCTTACGAACGACATCAATGACAGCGGCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCAt                                                                                                                                   <  1:1589617/150‑1 (MQ=255)
             gCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTATGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATa                                                                                                                                  <  1:698576/150‑1 (MQ=255)
             gCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATa                                                                                                                                  <  1:1682300/150‑1 (MQ=255)
              ccAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAg                                                                                                                                 <  2:1156293/150‑1 (MQ=255)
               cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAgg                                                                                                                                <  1:583897/150‑1 (MQ=255)
               cAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAgg                                                                                                                                <  1:545414/150‑1 (MQ=255)
                aaTACCTGAATTTAACACCAGGATATACAATGCGCCGCTCTTACGATCGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCTTAGGt                                                                                                                               <  1:1069264/150‑1 (MQ=255)
                 aTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTg                                                                                                                              <  2:1827689/150‑1 (MQ=255)
                   aCCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGCACGACATCAATGACAGCGTCTCTGTCAGCCCTATCTGGGAATACTGCGGTCGGTCCAGTCTCTTGTCCCAGGCGTTTAGCGGAATCTTTCGCCCGCGCAGAGGTgct                                                                                                                            >  2:288862/1‑148 (MQ=255)
                   aCCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGaa                                                                                                                            >  1:1519746/1‑150 (MQ=255)
                   aCCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACACCGCATAGGTGaa                                                                                                                            >  1:636031/1‑150 (MQ=255)
                    ccTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCTATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAAt                                                                                                                           <  2:1045213/150‑1 (MQ=255)
                    ccTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAAt                                                                                                                           >  1:1387474/1‑150 (MQ=255)
                    ccTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAAt                                                                                                                           >  2:444369/1‑150 (MQ=255)
                       gCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGt                                                                                                                        <  1:911178/150‑1 (MQ=255)
                        cATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGATTCTTTCGACCCCGCATAGGTGAATCGTa                                                                                                                       >  2:1402603/1‑150 (MQ=255)
                         aTTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTAc                                                                                                                      <  2:1002999/150‑1 (MQ=255)
                            tACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACAtt                                                                                                                   >  1:1173501/1‑150 (MQ=255)
                                accaGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGcc                                                                                                               <  1:25543/150‑1 (MQ=255)
                                    ggATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCAt                                                                                                           >  1:1639530/1‑150 (MQ=255)
                                        atCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGt                                                                                                       >  2:1910472/1‑150 (MQ=255)
                                          ccAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTgc                                                                                                     >  1:1148006/1‑149 (MQ=255)
                                          ccAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGa                                                                                                     >  1:1599953/1‑150 (MQ=255)
                                            aaTGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACg                                                                                                   <  2:409424/150‑1 (MQ=255)
                                                cgccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAac                                                                                               <  1:456097/150‑1 (MQ=255)
                                                 gccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacg                                                                                              <  2:376277/150‑1 (MQ=255)
                                                 gccgcTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCGCTTGAGCCAGGCGTTTAGCGGAATCTATCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacg                                                                                              <  1:79231/150‑1 (MQ=255)
                                                       ctTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGGGAATACTGCTGTCGCTCCAGGCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTAc                                                                                        <  1:502162/150‑1 (MQ=255)
                                                       attACGACCGACATCAATGACCGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGGGACGAAACGACGTAc                                                                                        <  2:227834/149‑1 (MQ=255)
                                                         tACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTATCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGa                                                                                      >  2:1864149/1‑150 (MQ=255)
                                                         tACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGa                                                                                      >  2:815943/1‑150 (MQ=255)
                                                         tACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGa                                                                                      >  1:1613594/1‑150 (MQ=255)
                                                          acgaacgaCATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGAt                                                                                     <  1:1859413/150‑1 (MQ=255)
                                                           cgaacgaCATCAATGACAGCGTCTCTGGCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATa                                                                                    <  2:270058/150‑1 (MQ=255)
                                                             aacgaCATCAATGACAGCGTCTCTGTCAGCACTATATGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGc                                                                                  <  2:1663608/150‑1 (MQ=255)
                                                              acgaCATCAATGACAGCGTATCTGTCAGCACTATCTGTTAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCg                                                                                 <  1:365108/150‑1 (MQ=255)
                                                                   aTCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAcc                                                                            >  2:1314358/1‑150 (MQ=255)
                                                                   aTCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCAAGTCTCTTGTGCCAGGCGGTTCGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCAcc                                                                            <  2:201415/150‑1 (MQ=255)
                                                                    tCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCg                                                                           <  1:981748/150‑1 (MQ=255)
                                                                     cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGCCCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGa                                                                          >  2:1293987/1‑150 (MQ=255)
                                                                     cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGa                                                                          >  2:1757320/1‑150 (MQ=255)
                                                                     cAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGa                                                                          >  2:160491/1‑150 (MQ=255)
                                                                      aaTGACAGCGTCTCTGTCAGCACTATCAGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGAt                                                                         >  1:1141013/1‑150 (MQ=255)
                                                                        tGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATAc                                                                       <  2:751998/150‑1 (MQ=255)
                                                                        tGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTACAGTCTCTTGTGCCAGGCCTTTAGCGGAATCTTTCGACCCCGCATAGGAGAATCGTACATTGGCCCCATCGGTGAAGAAACGACGAACTATAGCGGCACCTATAc                                                                       >  1:326584/1‑150 (MQ=255)
                                                                           cAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCAc                                                                    >  2:445267/1‑150 (MQ=255)
                                                                           cAGCGTCTCTGGCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCAc                                                                    <  2:1256476/150‑1 (MQ=255)
                                                                             gCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACgc                                                                  >  2:1235914/1‑149 (MQ=255)
                                                                              cGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGAc                                                                 <  2:1157092/150‑1 (MQ=255)
                                                                                tctcTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTg                                                               <  2:1276193/150‑1 (MQ=255)
                                                                                    tGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCAc                                                           >  1:662945/1‑150 (MQ=255)
                                                                                     gTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCAcc                                                          <  1:1505559/150‑1 (MQ=255)
                                                                                       cAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGa                                                        <  1:1425729/150‑1 (MQ=255)
                                                                                              ttCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgag                                                 <  2:439623/149‑1 (MQ=255)
                                                                                               tCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATgaga                                                >  1:1146409/1‑150 (MQ=255)
                                                                                                cTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGaa                                               <  2:331788/150‑1 (MQ=255)
                                                                                                cTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGaa                                               <  1:192338/150‑1 (MQ=255)
                                                                                                cTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGaa                                               >  2:1820580/1‑150 (MQ=255)
                                                                                                 tgtgAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGCCGAACCGCCGTACGATAGCGGCCCCGATCCCACGCCTGCCACCGCGGAGGAGAAc                                              >  2:1791224/1‑150 (MQ=255)
                                                                                                  gtgAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACt                                             >  1:985266/1‑150 (MQ=255)
                                                                                                   tgATTACTGGTGGCGCTCCAGTCTCTTGAGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGTCACCGATACCACGACTGCCACCGAGGATGAGAACTg                                            <  1:1039601/150‑1 (MQ=255)
                                                                                                   tgAATACTGCTGGCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTg                                            <  2:1512998/150‑1 (MQ=255)
                                                                                                    gAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGt                                           >  1:1111309/1‑150 (MQ=255)
                                                                                                    gAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAAAGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGt                                           >  2:1490532/1‑150 (MQ=255)
                                                                                                      aaacagctgCCGCTCCAGTCTCTTGTGCCAGCCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATATCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCt                                         <  2:1320861/145‑1 (MQ=255)
                                                                                                       tACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACGCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCTCCGATACCACGACTGCCACCGAGGATGAGAACTGTCtt                                        <  2:1599953/150‑1 (MQ=255)
                                                                                                         ctgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAc                                      >  2:1645221/1‑150 (MQ=255)
                                                                                                         ctgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAc                                      <  2:784561/150‑1 (MQ=255)
                                                                                                          tgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAcc                                     >  1:1924947/1‑150 (MQ=255)
                                                                                                          tgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAcc                                     <  2:1387474/150‑1 (MQ=255)
                                                                                                          tgctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGGGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTAcc                                     <  2:808376/150‑1 (MQ=255)
                                                                                                           gctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCt                                    >  2:1043084/1‑150 (MQ=255)
                                                                                                            ctgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTg                                   <  1:1858671/150‑1 (MQ=255)
                                                                                                             tgTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGCATCGTACATTGGCCCCATCGGTGACGAAACTCCGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGATAACTGTCTTACCTGt                                  >  2:960068/1‑150 (MQ=255)
                                                                                                              gTCGCTCCAGTCTCTTGTGCCCGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCCTCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTa                                 <  2:352350/150‑1 (MQ=255)
                                                                                                              gTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTa                                 >  2:755250/1‑150 (MQ=255)
                                                                                                              gTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTa                                 <  2:1606246/150‑1 (MQ=255)
                                                                                                                 gCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGCCTGCCACCGAGGATGAGAACTGTCTTAACTGTaaaa                              >  2:1188980/1‑150 (MQ=255)
                                                                                                                    ccAGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGACCTGTCTTACCTGTACAAgcg                           >  2:1505884/1‑150 (MQ=255)
                                                                                                                     cAGTCTCTTGTGCCAGGCGTCAAGCGGCATCATTCGACCCCGCATAGGAGAATCGAACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATTCCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAgcgc                          <  1:1226093/150‑1 (MQ=255)
                                                                                                                      aGTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGcc                         <  2:1421900/150‑1 (MQ=255)
                                                                                                                       gTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGccc                        <  1:85919/150‑1 (MQ=255)
                                                                                                                       gTCTCTTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGccc                        >  1:1793464/1‑150 (MQ=255)
                                                                                                                          tctTGTGCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATa                     <  2:1045757/150‑1 (MQ=255)
                                                                                                                             tgtgCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGGCTTACCTGTAAAAGCGCCCATAAgg                  >  1:379186/1‑150 (MQ=255)
                                                                                                                                gCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCCCGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACt               >  1:1173304/1‑150 (MQ=255)
                                                                                                                                gCCAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACAACGACTGCCACCGAGGATGAGAACTGTCTTACATGTAAAAGCGCCCATAAGGAcg               >  2:1238414/1‑149 (MQ=255)
                                                                                                                                 ccAGGCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTc              >  1:1840830/1‑150 (MQ=255)
                                                                                                                                    ggCGTTTAGCGGAATCTTTCGACCCCGCATCGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTATTACCTGTAAAAGCGCCCATAAGGACTCCtt           <  1:858849/150‑1 (MQ=255)
                                                                                                                                     gCGTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTg          >  1:611421/1‑150 (MQ=255)
                                                                                                                                       gTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAATGACTCCTTgct        >  2:758631/1‑148 (MQ=255)
                                                                                                                                       gTTTAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAt        >  1:683459/1‑150 (MQ=255)
                                                                                                                                         ttAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCTCCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAttt      >  1:1351209/1‑150 (MQ=255)
                                                                                                                                          tAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGAtttt     >  2:380746/1‑149 (MQ=255)
                                                                                                                                          tAGCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATtta     >  2:1733090/1‑150 (MQ=255)
                                                                                                                                            gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGCACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATttatt   >  2:1895972/1‑150 (MQ=255)
                                                                                                                                            gCGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGCCTCCTTGATttatt   >  1:792324/1‑150 (MQ=255)
                                                                                                                                             cGGAATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAACGCGCCCATAAGGACTCGTTGATttatta  >  2:1765028/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTACGATAGCGGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTA  >  NC_000913/4473783‑4474073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: