Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,157 A→T 5.5% intergenic (‑129/+301) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,1570AT 5.5% 1216.7 / 13.5 428intergenic (‑129/+301)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base A (230/153);  new base T (2/33);  total (233/195)
Fisher's exact test for biased strand distribution p-value = 9.94e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 439 total aligned reads displayed.

ACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTT  >  NC_000913/3392008‑3392306
                                                                                                                                                     |                                                                                                                                                     
 cTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                                                                                                      <  2:3850752/150‑1 (MQ=255)
  ttACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAgg                                                                                                                                                     <  1:2898900/150‑1 (MQ=38)
   tACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAGGc                                                                                                                                                    <  2:4297933/150‑1 (MQ=37)
    aCCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATGCGACGCTGTGCGTCTTATCAGGcc                                                                                                                                                   <  1:2090290/150‑2 (MQ=37)
      cAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                                                                                                                                 >  2:3424518/1‑150 (MQ=255)
              gACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCg                                                                                                                                         <  2:3222428/150‑1 (MQ=255)
              gACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCg                                                                                                                                         <  2:5408806/150‑1 (MQ=255)
                   aTCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATgc                                                                                                                                    <  2:4844388/150‑1 (MQ=255)
                    tCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAATACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATgcg                                                                                                                                   >  1:1855682/1‑150 (MQ=255)
                          ttGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTACCCAGCCTACGGTTATGTTCCGGTTTGTAGGACTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTaa                                                                                                                             >  1:3703360/1‑150 (MQ=255)
                            gCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGATAAGTTCCGGTTTGTAGGACTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAAc                                                                                                                           >  1:5166026/1‑150 (MQ=255)
                               aCTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGcc                                                                                                                        <  2:1352554/150‑1 (MQ=255)
                               aCTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGcc                                                                                                                        <  2:4099070/150‑1 (MQ=255)
                                 tCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGATCCGGTTAGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCtt                                                                                                                      >  1:5969524/1‑150 (MQ=255)
                                 tCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGTAAACGCCtt                                                                                                                      >  2:4761766/1‑150 (MQ=255)
                                 tCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCGATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCtt                                                                                                                      <  2:2917584/150‑1 (MQ=255)
                                   gttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTAt                                                                                                                    >  1:2747728/1‑150 (MQ=255)
                                    ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                   >  1:771636/1‑150 (MQ=255)
                                    ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                   <  2:1729724/150‑1 (MQ=255)
                                    ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCAGGATAGTAGGCCTGATAAAACGAAAACCGAAGAAACACGCAACGGCACACGGTGGCGCCGAAAACGcataaac                                                                                                                   >  2:2488214/1‑144 (MQ=16)
                                       gttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGa                                                                                                                >  1:3383901/1‑150 (MQ=255)
                                        ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                                               <  2:3650329/150‑1 (MQ=255)
                                        ttaCGGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAACCGCCTTATCCGAc                                                                                                               <  2:2113890/150‑1 (MQ=255)
                                         taCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                              >  1:1791583/1‑150 (MQ=255)
                                          aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCt                                                                                                             >  1:5218688/1‑150 (MQ=255)
                                          aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATCCGGCGTAAACGCCTTATCCGACCt                                                                                                             >  1:3415739/1‑150 (MQ=255)
                                            tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAc                                                                                                           >  1:1009364/1‑150 (MQ=255)
                                              ctgttgagGATTTTTTCCGGCTGCGGGAGGCGGCGTGAACGCCCTATCCAGCCTAGGTTTATGTTCCGGTTTGTCGGCCTGATAAGACGCACCGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                         <  1:3964363/145‑1 (MQ=17)
                                              ccgTAGTGGTTTTTTTCGGGCTGCGGGTTGGGGGGTGAAGGCCCTATCCCGCCTAGGGTTCTGTTCCGGTTTGTCGGCCTGATACGACGCCCCGGGTCGCATCCGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                         <  1:3202407/148‑1 (MQ=17)
                                               aGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAATCGCCTTATCCGACCTACggg                                                                                                        >  1:4242624/1‑149 (MQ=255)
                                               aGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGTCAACGCCTTAGCCGACCTACGGt                                                                                                        >  1:5782703/1‑150 (MQ=255)
                                                  agagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                                                                                     <  2:3267201/150‑1 (MQ=255)
                                                      gATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTCTGTTCCGGTTTGTAGGCCTGATAAGACGCAAAGCGTCGCATCAGGCACCGGCTGTCGGAGGCGGCGTAAACGCCGTATCCGACCTACGGGTATGTTc                                                                                                 >  1:2040225/1‑150 (MQ=17)
                                                         tttttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGt                                                                                              >  1:1310929/1‑150 (MQ=255)
                                                         tttttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTAGTAGGCCTGATAAGACGAAAAGCGTCGCATCAGGCAACGGATGACCGAACAGGCGTAAACGCAATATCCTACCTACGGTTATGTTCCGt                                                                                              >  2:4496/1‑150 (MQ=25)
                                                           tttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                            <  2:3542464/150‑1 (MQ=255)
                                                           tttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                            >  1:3831839/1‑150 (MQ=255)
                                                           tttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                            >  2:3327807/1‑150 (MQ=255)
                                                           tttACGGCTGCCGGAGGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                            <  1:4505850/150‑1 (MQ=255)
                                                            ttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGGTATGTTCCGTTTg                                                                                           >  1:1824475/1‑150 (MQ=255)
                                                              aCGGCGGCCGGATGCGGCGTGAACGCCCTATCCAGCCAACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGGCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                                                         <  1:899677/150‑1 (MQ=255)
                                                                 gCTGCCGGTTGCGTCGTTAACGCCCTTTCCAGACTACGGTTATTTTCCGTTTTGTTGGCCTGATAAGCCGCTCCGCGTGGCAACAGGAAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                                                      <  1:4461388/150‑1 (MQ=14)
                                                                  cTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                                                                                     >  1:2469483/1‑150 (MQ=255)
                                                                   tggggtaTGGGGCGTGAACCCCCTATACTGCCTACGGTTTTGTGCCGGTTTTTAGGCATGTTAAGACGCACATCGCCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                    <  1:4080808/144‑1 (MQ=14)
                                                                   tGCCGGTTGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                    <  2:224778/150‑1 (MQ=255)
                                                                   tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGGTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCCTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                    <  2:3471221/150‑1 (MQ=255)
                                                                   tGCCGGAGGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCCGATAAGACGCACAGCGTCGCAACAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                    <  2:1239640/150‑1 (MQ=255)
                                                                     ccGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                                                  >  1:3661179/1‑150 (MQ=255)
                                                                         acgcggcgTGAACGCCCTATCCAGCCGACGGTTATGTTCCGGTTTGTAGGCGTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAg                                                                              <  2:5877330/148‑1 (MQ=255)
                                                                          tggggcgTGAACGCCCTCTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                                                                             <  1:4615208/147‑1 (MQ=255)
                                                                          tGCGGCTTGAACGCCCTACCCAGCCTACGGTTTTGTGCCGGTTTGTAGGCCGGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                                                                             <  1:5076655/150‑1 (MQ=14)
                                                                          tGCGGCGTGACCGCCCTATCCAGCCTACGGTTATGTTCGGGTTTGTAGGCCTTATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                                                                             <  1:1554999/150‑1 (MQ=255)
                                                                           gcggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAc                                                                            >  1:2208526/1‑150 (MQ=255)
                                                                            cggcgTGCACGCCCTCTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCGGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg                                                                           <  2:4954081/150‑1 (MQ=255)
                                                                            cggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg                                                                           <  2:3886808/150‑1 (MQ=255)
                                                                            cggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg                                                                           >  2:4614584/1‑150 (MQ=255)
                                                                             ggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAAGTTCCGTTTGTAGGCCTGATAAGACGc                                                                          >  1:5611020/1‑150 (MQ=255)
                                                                                     cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                  <  2:3498772/150‑1 (MQ=255)
                                                                                     cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                  >  2:3087140/1‑150 (MQ=255)
                                                                                     cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCGTTTGTATGCCTGATCACACGCACAgagac                                                                  >  2:1576452/1‑146 (MQ=14)
                                                                                       cccTATCCAGCCTAGGGTTATGTTCCGGTTTGTAGGCTTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                <  1:5684229/150‑1 (MQ=255)
                                                                                         cTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                                              >  1:631370/1‑150 (MQ=255)
                                                                                         cTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTGTGTAGGCCTGATAAGACGCCCAGCGgcgctg                                                              >  2:1853633/1‑148 (MQ=255)
                                                                                             ccAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAGAAGACGCACAGCGTCGCATCAgg                                                          >  2:5044378/1‑150 (MQ=255)
                                                                                                gCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCTTTTGAAGGCCTGAAAAGACGCACAGCGTCGCATCAGGCaa                                                       >  1:1162750/1‑150 (MQ=255)
                                                                                                 cctcgggTTATTTACCGTTTTGTCGTCCTGATAAGACGCGCAGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACATACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                                      <  2:4037492/145‑1 (MQ=11)
                                                                                                 ccTACGGTTATGTTCCGGTTTGTTGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                                      <  1:5377604/150‑1 (MQ=255)
                                                                                                 ccTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc                                                      >  2:76096/1‑150 (MQ=37)
                                                                                                       gTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCTTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGc                                                >  1:2308197/1‑150 (MQ=37)
                                                                                                        ttATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGcc                                               >  2:575231/1‑150 (MQ=37)
                                                                                                        ttATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGcc                                               >  1:2284831/1‑150 (MQ=37)
                                                                                                        ttATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGcc                                               >  1:2988310/1‑150 (MQ=17)
                                                                                                        gtATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCCGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGcc                                               >  2:2601913/2‑150 (MQ=37)
                                                                                                            gTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAt                                           >  2:5582989/1‑150 (MQ=37)
                                                                                                             ttCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATg                                          >  1:139360/1‑150 (MQ=35)
                                                                                                             gtCCGGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGAGGCGGCGTAAACGCCTTATCCGACCTCCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATg                                          <  2:812017/149‑1 (MQ=255)
                                                                                                               ccGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                                                                                     >  1:4709219/1‑41 (MQ=38)
                                                                                                                cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                                                                                                      <  2:4363519/39‑1 (MQ=37)
                                                                                                                cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                                       >  1:215012/1‑150 (MQ=33)
                                                                                                                  gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggcg                                     >  2:46870/1‑150 (MQ=32)
                                                                                                                      gttggCCTGATAAGACGCACCGCGTCGCATCAGGAAACGGCTGGCGGATGCGGCGTAAACGCCTTAGCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTaaa                                 <  2:208501/147‑1 (MQ=11)
                                                                                                                          gCCTGATACGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGCCCTCCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGcc                             <  2:5949031/150‑1 (MQ=255)
                                                                                                                            cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGccct                           >  1:2969725/1‑148 (MQ=17)
                                                                                                                            cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCtt                           >  1:1978666/1‑150 (MQ=35)
                                                                                                                            cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCAGTATGTAGGCCAGATAAGACGCACAGAGTCGCATCATGCAACGGCTGCCGGATGCGGCGTAAACGCCtt                           >  1:4323732/1‑150 (MQ=11)
                                                                                                                             tGATAAGACGCACAGCGTCGCATCAGGCACCGGCTGTCGGATGCGGCGTAAACGCCGTCTCCGACCTACGGTGATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCCGGCAACGGCTGCCGGCTGCGGCGGCAACGccggc                          >  2:4195126/1‑147 (MQ=11)
                                                                                                                             tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTa                          >  1:2590261/1‑150 (MQ=37)
                                                                                                                             tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTa                          >  2:1052361/1‑150 (MQ=37)
                                                                                                                             tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAGGCGGCGTAAACGCCTTa                          >  2:5632450/1‑150 (MQ=37)
                                                                                                                              gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACGGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTAt                         >  1:5532550/1‑150 (MQ=37)
                                                                                                                              gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGGTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCACCGGCTGCCGGAGGCGGCGTCAACGCCttcg                         >  2:4711147/1‑148 (MQ=11)
                                                                                                                                    aCGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCCTCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTGAGCCGAcc                   >  2:3685799/1‑150 (MQ=11)
                                                                                                                                    aCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGCACAGCGACGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTGATCCGAcc                   >  2:3134357/1‑150 (MQ=255)
                                                                                                                                     cGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCt                  >  1:5894635/1‑150 (MQ=37)
                                                                                                                                            cGTCGCTTCTGGCAACGGCTGTGGGATGCGGCTTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTa           <  2:1817381/150‑1 (MQ=11)
                                                                                                                                             ggcggaTCTGGCACGGGCTGTCGGTTGGGGCGTAACGGCCTTATCCGCCCTCGGGTTTTGTGCGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt          <  2:1925700/145‑1 (MQ=11)
                                                                                                                                             gTCGCATCAGGCAACGGCTGTGGGATGCGGGGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt          <  2:5263702/150‑1 (MQ=255)
                                                                                                                                                 cATCAGGCACCGGCTGGCGGAGGCGGCGTAACCGCCTTACCCGACCTAGGGTTATGTTCCGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTc      <  2:2747728/150‑1 (MQ=11)
                                                                                                                                                 cATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTc      >  1:3603268/1‑150 (MQ=37)
                                                                                                                                                     aGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAAGGTTATGTTCCGTGTGTTGTCCATTTAAGACGCACAGCGTCGCATCAGGCACCGGCTGCCGGATGCGGCGTAAACGCCTTAGCCGACCGACGATTATGTTCCGtt  >  2:5516511/1‑150 (MQ=14)
                                                                                                                                                     |                                                                                                                                                     
ACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTT  >  NC_000913/3392008‑3392306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: