Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,482,325 G→A 100% R346H (CGC→CAC)  yheS → ABC‑F family protein predicted regulatory ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,482,3250GA100.0% 1488.0 / NA 388R346H (CGC→CAC) yheSABC‑F family protein predicted regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (192/196);  total (192/196)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 390 total aligned reads displayed.

CGTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTA  >  NC_000913/3482178‑3482471
                                                                                                                                                   |                                                                                                                                                  
cGTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCcaca                                                                                                                                                  >  1:82999/1‑150 (MQ=255)
  ttCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAAt                                                                                                                                                <  1:5358566/150‑1 (MQ=255)
       cTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGgcgc                                                                                                                                           <  2:3736631/150‑1 (MQ=255)
        tttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGcgcg                                                                                                                                          <  2:1558553/150‑1 (MQ=255)
        tttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGcgcg                                                                                                                                          >  2:107630/1‑150 (MQ=255)
        tttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGcgcg                                                                                                                                          <  2:5844601/150‑1 (MQ=255)
         ttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCgg                                                                                                                                         >  2:72356/1‑150 (MQ=255)
              ttCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaaa                                                                                                                                    >  1:1797383/1‑150 (MQ=255)
               tCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAt                                                                                                                                   >  1:4468106/1‑150 (MQ=255)
                  gcgcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGTTGGAAATAGTCTGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGa                                                                                                                                <  2:4489139/150‑1 (MQ=255)
                    gcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACa                                                                                                                              <  1:5537579/150‑1 (MQ=255)
                          gAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATc                                                                                                                        <  2:1499916/150‑1 (MQ=255)
                          gAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATc                                                                                                                        <  2:1253425/150‑1 (MQ=255)
                          gAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATc                                                                                                                        >  1:4661703/1‑150 (MQ=255)
                          gAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATc                                                                                                                        >  2:596320/1‑150 (MQ=255)
                               ccTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACt                                                                                                                   >  1:965890/1‑150 (MQ=255)
                                cTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTg                                                                                                                  >  1:2096093/1‑150 (MQ=255)
                                 tGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGt                                                                                                                 >  1:5893257/1‑150 (MQ=255)
                                 tGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGt                                                                                                                 >  2:400699/1‑150 (MQ=255)
                                   ccAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTa                                                                                                               >  1:3345284/1‑150 (MQ=255)
                                   ccAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTa                                                                                                               <  2:2432711/150‑1 (MQ=255)
                                    cAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAg                                                                                                              >  2:2287875/1‑150 (MQ=255)
                                     aaaTCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGc                                                                                                             <  1:1731315/150‑1 (MQ=255)
                                        tCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCgg                                                                                                          <  2:565806/150‑1 (MQ=255)
                                        tCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCgg                                                                                                          <  1:5126247/150‑1 (MQ=255)
                                        tCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCgg                                                                                                          <  1:1467971/150‑1 (MQ=255)
                                        tCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCgg                                                                                                          <  2:4156434/150‑1 (MQ=255)
                                           gTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGa                                                                                                       >  2:3863900/1‑150 (MQ=255)
                                           gTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGa                                                                                                       >  1:5799043/1‑150 (MQ=255)
                                           gTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGa                                                                                                       >  2:5747445/1‑150 (MQ=255)
                                            ttACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGaa                                                                                                      <  1:1187997/150‑1 (MQ=255)
                                             tACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAAc                                                                                                     <  2:3180275/150‑1 (MQ=255)
                                               cTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACtt                                                                                                   >  1:2328588/1‑150 (MQ=255)
                                                tGAAGATGGAAAAAGACAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTg                                                                                                  >  2:2079225/1‑150 (MQ=255)
                                                 gAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgc                                                                                                 >  2:2756060/1‑150 (MQ=255)
                                                 gAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgc                                                                                                 >  1:1870401/1‑150 (MQ=255)
                                                  aaGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgcg                                                                                                <  2:3687065/150‑1 (MQ=255)
                                                   aGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgcgc                                                                                               <  1:612196/150‑1 (MQ=255)
                                                    gATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGcc                                                                                              >  1:4595591/1‑150 (MQ=255)
                                                    gATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGcc                                                                                              >  1:5220682/1‑150 (MQ=255)
                                                      tGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAg                                                                                            >  2:2902253/1‑150 (MQ=255)
                                                       ggAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGt                                                                                           <  1:4093128/150‑1 (MQ=255)
                                                        gAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTc                                                                                          >  2:5571319/1‑150 (MQ=255)
                                                               tCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTg                                                                                   >  2:5273793/1‑150 (MQ=255)
                                                               acAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTg                                                                                   >  1:3010682/2‑150 (MQ=255)
                                                                 aGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGaa                                                                                 >  2:3611846/1‑150 (MQ=255)
                                                                  gcgcGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGaaa                                                                                <  2:4796608/150‑1 (MQ=255)
                                                                  gcgcGGGCGAGGGCGATCGCATGATTCTCGACTCGATTAAACTGAACCTGGGGCCCGGCTCGCGTATTGGTCTGTTAGGCCACACTGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGaaa                                                                                <  2:4719316/150‑1 (MQ=255)
                                                                    gcgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAAtt                                                                              <  1:4403679/150‑1 (MQ=255)
                                                                       ggCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGt                                                                           >  2:4753558/1‑150 (MQ=255)
                                                                        gCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTc                                                                          <  1:3141369/150‑1 (MQ=255)
                                                                         cTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCt                                                                         <  1:1287837/150‑1 (MQ=255)
                                                                          tATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTg                                                                        >  1:1078706/1‑150 (MQ=255)
                                                                           aTGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTgg                                                                       <  2:641065/150‑1 (MQ=255)
                                                                              gCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGa                                                                    >  1:4071422/1‑150 (MQ=255)
                                                                               cGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaa                                                                   >  1:2272603/1‑150 (MQ=255)
                                                                                gATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                  >  1:282374/1‑150 (MQ=255)
                                                                                 aTCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAg                                                                 >  2:345430/1‑150 (MQ=255)
                                                                                    gCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGa                                                              >  1:330366/1‑150 (MQ=255)
                                                                                    gCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGa                                                              <  1:4241728/150‑1 (MQ=255)
                                                                                     cATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGAt                                                             <  2:4632065/150‑1 (MQ=255)
                                                                                      attattCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATc                                                            <  2:5211574/150‑1 (MQ=255)
                                                                                         attCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCaaa                                                         <  2:395929/150‑1 (MQ=255)
                                                                                         attCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCaaa                                                         <  1:2661422/150‑1 (MQ=255)
                                                                                            ctcgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTc                                                      <  1:2170378/150‑1 (MQ=255)
                                                                                             tcgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCATATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCg                                                     >  1:2487120/1‑150 (MQ=255)
                                                                                               gactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGc                                                   <  2:3082137/150‑1 (MQ=255)
                                                                                               gactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGc                                                   <  2:5461122/150‑1 (MQ=255)
                                                                                                  tcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTAc                                                <  2:4929109/150‑1 (MQ=255)
                                                                                                   cgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACt                                               <  2:3513874/150‑1 (MQ=255)
                                                                                                     aTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTc                                             >  2:2705976/1‑150 (MQ=255)
                                                                                                     aTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTc                                             >  2:1901295/1‑150 (MQ=255)
                                                                                                           cTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAg                                       >  2:1825390/1‑150 (MQ=255)
                                                                                                            tGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGc                                      <  2:813535/150‑1 (MQ=255)
                                                                                                            tGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGc                                      <  1:3452197/150‑1 (MQ=255)
                                                                                                                 cTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCaa                                 >  1:5974559/1‑150 (MQ=255)
                                                                                                                  tGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAAc                                <  1:5700387/150‑1 (MQ=255)
                                                                                                                    gTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACtt                              <  1:3823220/150‑1 (MQ=255)
                                                                                                                    gTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACtt                              <  1:5397713/150‑1 (MQ=255)
                                                                                                                        ccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAAt                          >  2:4684065/1‑150 (MQ=255)
                                                                                                                          ggCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATAc                        <  1:575052/150‑1 (MQ=255)
                                                                                                                          ggCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATAc                        <  2:3391124/150‑1 (MQ=255)
                                                                                                                            cTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCt                      <  2:1363554/150‑1 (MQ=255)
                                                                                                                              cgcgTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTgc                    >  2:5421505/1‑150 (MQ=255)
                                                                                                                               gcgTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGGATACCTgcg                   >  1:1527948/1‑150 (MQ=255)
                                                                                                                                  tATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTgcgcgc                >  2:5055410/1‑150 (MQ=255)
                                                                                                                                   aTTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGcc               <  1:5712567/150‑1 (MQ=255)
                                                                                                                                     tGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcga             <  2:3159036/150‑1 (MQ=255)
                                                                                                                                     tGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcga             <  2:4849336/150‑1 (MQ=255)
                                                                                                                                     tGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcga             <  2:1938742/150‑1 (MQ=255)
                                                                                                                                      ggTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgac            <  1:4989146/150‑1 (MQ=255)
                                                                                                                                      ggTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgac            <  1:3915865/150‑1 (MQ=255)
                                                                                                                                      ggTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgac            <  1:3687614/150‑1 (MQ=255)
                                                                                                                                       gTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacg           >  2:408987/1‑150 (MQ=255)
                                                                                                                                          tGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAAt        <  1:2880753/150‑1 (MQ=255)
                                                                                                                                          tGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAAt        >  2:4570851/1‑150 (MQ=255)
                                                                                                                                             tAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAc     <  2:467089/150‑1 (MQ=255)
                                                                                                                                                gCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTa  >  1:1570938/1‑150 (MQ=255)
                                                                                                                                                gCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTa  >  1:1018503/1‑150 (MQ=255)
                                                                                                                                                gCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTa  >  1:3651172/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTA  >  NC_000913/3482178‑3482471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: