Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,044 T→A 16.7% intergenic (+27/‑171) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0440TA16.7% 668.2 / 52.6 264intergenic (+27/‑171)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (32/177);  new base A (41/2);  total (84/180)
Fisher's exact test for biased strand distribution p-value = 1.21e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

GTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCT  >  NC_000913/3780895‑3781192
                                                                                                                                                     |                                                                                                                                                    
 tGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCctc                                                                                                                                                     <  1:2089052/150‑1 (MQ=255)
        gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa                                                                                                                                              <  1:5914295/150‑1 (MQ=255)
        gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa                                                                                                                                              <  2:3129738/150‑1 (MQ=255)
         aCGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                                             <  1:1428660/150‑1 (MQ=255)
          cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                                                                            <  1:5058145/150‑1 (MQ=255)
            cTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGga                                                                                                                                          <  2:1520744/150‑1 (MQ=255)
              gACTGGCGCGAAATCGATCAGCGAAATTACGCAAGTTTCGCTGGTGGAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTGCGATGGCGAAAGGGTATGCGGCATAGTCGTTTGCGCCCCCTCACCGTAACCCTCTCGCTCTGGgtga                                                                                                                                        <  2:661353/150‑1 (MQ=255)
                cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTTGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                      <  1:995128/150‑1 (MQ=255)
                cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                      <  1:3045313/150‑1 (MQ=255)
                cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGGTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                      <  1:1462813/150‑1 (MQ=255)
                 tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                                                     <  1:1680204/150‑1 (MQ=255)
                 tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                                                     <  1:1403262/150‑1 (MQ=255)
                 tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                                                     <  1:4748646/150‑1 (MQ=255)
                   gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCCAACCCACACCCACACGGCGCggggc                                                                                                                                   >  2:4179739/1‑149 (MQ=255)
                    cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGAc                                                                                                                                  <  2:4007132/150‑1 (MQ=255)
                     gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggttag                                                                                                                                 <  2:4459653/150‑5 (MQ=255)
                     gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggttag                                                                                                                                 <  1:1586770/150‑5 (MQ=255)
                      cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgggttagg                                                                                                                                <  2:326967/150‑6 (MQ=255)
                        aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggttagggt                                                                                                                              <  2:1994919/150‑8 (MQ=255)
                        aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                                                                              <  2:1174514/150‑1 (MQ=255)
                           tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGGGCCCCCCCACCCCAACCCACTCCCGCCGGGCGGGGGGCCCgggcgg                                                                                                                           >  2:2858494/1‑145 (MQ=255)
                           tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCACCCAAACCACACCCACAAGGGGAGGGGACCCGTCgg                                                                                                                           >  1:1063490/1‑150 (MQ=255)
                            cGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                                                                          <  2:4538050/150‑1 (MQ=255)
                             gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTCGCCCCCACCCCCCAAACCCAACCCACACGGGGGGGGGCCCCGTCGgcg                                                                                                                         >  2:2748465/1‑150 (MQ=255)
                             gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCGAACCCACGACCCCCGGGGGAGGGGGCCGGTCGgcg                                                                                                                         >  1:2776552/1‑150 (MQ=255)
                             gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGGGCGCCCCCGCCCCCGCACCCACACCCGCACGGCGGGGGGGCCgggccgcg                                                                                                                         >  2:3157985/1‑143 (MQ=255)
                              aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                                                                        <  1:3077647/150‑1 (MQ=255)
                               tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                       <  2:917329/150‑1 (MQ=255)
                               tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                       <  2:1961257/150‑1 (MQ=255)
                                cAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                                                                      <  2:2145943/150‑1 (MQ=255)
                                cAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                                                                      <  1:5235958/150‑1 (MQ=255)
                                 aGCGAACTTCCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTGGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCtgt                                                                                                                     <  1:5162447/150‑1 (MQ=255)
                                 aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCTAAACCACACCCACACGGGGAGGGGACCCGTCGGCGCagt                                                                                                                     >  1:3836305/1‑150 (MQ=255)
                                 aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCCAAACCACACCCACAGGGGGAGGGGGCCCGTCgggggggg                                                                                                                     >  1:76739/1‑144 (MQ=255)
                                    gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                                                                                  <  1:4231561/150‑1 (MQ=255)
                                      aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatgta                                                                                                                <  1:3265895/150‑1 (MQ=255)
                                       aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTAc                                                                                                               <  1:3481437/150‑1 (MQ=255)
                                       aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTAc                                                                                                               <  2:597091/150‑1 (MQ=255)
                                       aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTAc                                                                                                               <  1:2180262/150‑1 (MQ=255)
                                       aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACCACCAAACCCACTACCACAGGGGGAGGGGGCCCGTCGGGGCtgtgtgttc                                                                                                               >  1:4243190/1‑148 (MQ=255)
                                          aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                                                                            <  1:4874077/150‑1 (MQ=255)
                                          aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                                                                            <  2:4814330/150‑1 (MQ=255)
                                          aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                                                                            <  2:5746956/150‑1 (MQ=255)
                                           cGCAAGATTCGCTGGTGCAGGGGCTGGGTACAGAGGTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAAGGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc                                                                                                           <  2:4134621/150‑1 (MQ=255)
                                             cAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTc                                                                                                         <  2:5419027/150‑1 (MQ=255)
                                             cAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACAACCCAACCCACAACCCCAGGGGGAGGGGCCCCGTGGGCGCGGGATGTACAcccac                                                                                                         >  2:2821230/1‑148 (MQ=255)
                                                 aTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc                                                                                                     <  1:2499319/150‑1 (MQ=255)
                                                   tCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTg                                                                                                   <  2:5047335/150‑1 (MQ=255)
                                                     gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaa                                                                                                 <  1:5387651/150‑1 (MQ=255)
                                                      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCAAACCCTCTCCCACAGGGCGTGGGGGCCGGTCGGCGCTGTATGTACTCCCTCACACTGgaa                                                                                                >  1:4969174/1‑150 (MQ=255)
                                                      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                <  2:2274021/150‑1 (MQ=255)
                                                      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACTCCCTCAGGGGGGGGGGGCCGTTAGGCGCGGTATGTACTCCCGCACACTGaaa                                                                                                >  2:5917129/1‑150 (MQ=255)
                                                      cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAAACCAAAACCTACGGGTGAGGGGACCGGTCGGCGCGGGAAGTACACCCGCActccgtaa                                                                                                >  2:3005051/1‑145 (MQ=255)
                                                       tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                               <  2:4455063/150‑1 (MQ=255)
                                                               gggCTGGGTAATGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGACACGACACCGc                                                                                       <  1:1785714/150‑1 (MQ=255)
                                                                 gCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCCCCCCCCCACCCAAAAACACAACCCCACCGCGAGGGGGCCCATAGGGGCGGTATGTACAACCACACACAGAAAAAACACCCCAc                                                                                     >  1:688600/1‑150 (MQ=255)
                                                                   tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCActc                                                                                   <  2:3757717/150‑1 (MQ=255)
                                                                   tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCActc                                                                                   <  2:1962910/150‑1 (MQ=255)
                                                                   tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAAACCACACCCACCGGGGGGGGGGGCCGGTCGGCGCGGTATGTACAACCGCACACAGAAAAGACACCGcccgc                                                                                   >  1:2988029/1‑146 (MQ=255)
                                                                   tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAAACCACACCCGCAGGGGGAGGGGTCCGGTCGGCGCGGGTTTTAATCCCGCACCCGGGAACGACAccccccac                                                                                   >  1:2703702/1‑144 (MQ=255)
                                                                      gTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAAACCAAACCCACACGGGGAGGGGCCCCGTCGGCGCGGTATGTAAACCCCCACACGGGAACCACACCCCACACttt                                                                                >  2:4402253/1‑150 (MQ=255)
                                                                       tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCGAAACCACACCCGCAGGGGGGGGGGACCGGTGGGGGCTGTTTTTACTCCCGCACACATAAACGACACCCCCCACtttt                                                                               >  2:2445018/1‑150 (MQ=255)
                                                                          agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                            <  1:3712153/150‑1 (MQ=255)
                                                                            agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                          <  2:3858344/150‑1 (MQ=255)
                                                                             gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                         <  2:743881/150‑1 (MQ=255)
                                                                              ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                        <  1:1848307/150‑1 (MQ=255)
                                                                                gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCt                                                                      <  1:5240359/150‑1 (MQ=255)
                                                                                 ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                     <  1:3548699/150‑1 (MQ=255)
                                                                                 ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                     <  2:1328388/150‑1 (MQ=255)
                                                                                  cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                                    <  2:2932226/150‑1 (MQ=255)
                                                                                   tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCCCACCCAAAACCACAACCCCAGGGGGAGGGGACCGGTCGGCGCAGTTTGTTAAAACGCACACTGAAAAGACACCGCCAACGTTTTTTATCCCACGc                                                                   >  1:3363071/1‑150 (MQ=255)
                                                                                   tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                                                   <  1:2627575/150‑1 (MQ=255)
                                                                                           tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                           <  1:2595361/150‑1 (MQ=255)
                                                                                            ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                          <  2:1887046/150‑1 (MQ=255)
                                                                                             gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCTCAACCAAAGGGAGAGGGGTCCCTTAGGCGCGGTATTTAATCCCTCACAATGTAACCAACCCCCACACTTTTTTTATAACACGCCCCACCgggg                                                         >  2:3351939/1‑150 (MQ=255)
                                                                                              cgcccATGGCGAAAGGGCAGGCGGCATCGGCGTGTGCGCCCCCCCCCCCTACCCCCCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACACTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                        <  1:849722/148‑1 (MQ=255)
                                                                                                cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                      <  1:1172429/150‑1 (MQ=255)
                                                                                                 ccATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACCCCCGAAACCGCAACCTAAGGGCGAGGGGCCCGGTCGGCGCGGTATGTAAGCCCGCACTCTGAAACGACACCGCCCACGTTTTTTCTCCCGCGCCCCGCCGGGGcgag                                                     >  2:4980249/1‑150 (MQ=255)
                                                                                                   aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                                                   <  1:2929311/150‑1 (MQ=255)
                                                                                                       cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                               <  2:3113602/150‑1 (MQ=255)
                                                                                                       cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                               <  1:3746448/150‑1 (MQ=255)
                                                                                                         aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAACCCACACCCGCAGGGCGAGGGGGCCCGTCGGCGCTGGTTGTACAACCACACAAGGAAAAGCCACCCCACTCATTTTTTATACCACGCCCCCCCCGGGCGAGGGCCcggg                                             >  2:5307419/1‑150 (MQ=255)
                                                                                                          aaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                            <  1:3329495/150‑1 (MQ=255)
                                                                                                          aaGGGAATGCGGCATAGTCGTGTTCGCCCCCGCACCCAAAACCTATCCCACCGGGGGAGGGGTCCGGTCGGGGGGGTTGGTACACCCACACGCGGAAACCACCCCCCCCTCATTTTTTAGCCCTCGCCCCAACGGGGCGAGGGGCggggg                                            >  2:3619840/1‑149 (MQ=255)
                                                                                                           aGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCGAAACCGCTCCCGCAGGGAGAGGGGACCGTTCGGCGCTGTTTGTACGCCCTCACGCGGGAACCACCCCGCACACTTTTTTTATCCCGCGCCCCGCCGGGGGGTGGGCCgggggg                                           >  2:3938991/1‑148 (MQ=255)
                                                                                                             ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                         <  2:5079769/150‑1 (MQ=255)
                                                                                                              gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                        <  1:3971909/150‑1 (MQ=255)
                                                                                                               aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                       <  2:316163/150‑1 (MQ=255)
                                                                                                                    ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                  <  2:2395059/150‑1 (MQ=255)
                                                                                                                    ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                  <  2:2336362/150‑1 (MQ=255)
                                                                                                                    ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCATTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                  <  2:3504109/150‑1 (MQ=255)
                                                                                                                     gCATAGTCGTTTGCGCCCCCACACCCTAAACCAAACCCACAGGGAGAGGGGACCCTTCGGCGCGGTAAGTACACCCTCACAAATAAAAGACACCCCACTCTTTTTTTATCCCACGCCCCACCCGGGAGAGGGCCGGGGGGAGGGGaaaat                                 >  2:985957/1‑149 (MQ=255)
                                                                                                                      cATAGTCGTTTGCGCCCCCACACCCAAAAACAAACCCGCACGGAGAGGGGAAACGGCGGGGCGGTTTGTAAACCCTCACTCTGAAACCTCACCCCACGCATTTTTTCTCACGCGCCCCCCCGGGGCGCGGGGCGGGGTGGGGGGAAAAgg                                >  2:772858/1‑150 (MQ=255)
                                                                                                                             gTTTGCACCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCAcg                         >  2:3783139/1‑149 (MQ=255)
                                                                                                                                   gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACCCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                   >  2:1643743/1‑150 (MQ=255)
                                                                                                                                           aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTc           >  2:5484846/1‑150 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg         >  2:5864623/1‑150 (MQ=255)
                                                                                                                                                tccccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGGTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTa      >  2:4338517/3‑150 (MQ=255)
                                                                                                                                                  cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATc    >  1:4515816/1‑150 (MQ=255)
                                                                                                                                                    ctctCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt  >  2:1823380/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCT  >  NC_000913/3780895‑3781192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: