Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,094,415 T→G 5.5% intergenic (‑143/+308) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,094,4150TG 5.5% 1242.7 / 46.5 417intergenic (‑143/+308)rhaD/rhaArhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base T (154/231);  new base G (17/8);  total (176/241)
Fisher's exact test for biased strand distribution p-value = 1.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 425 total aligned reads displayed.

TTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCT  >  NC_000913/4094266‑4094564
                                                                                                                                                     |                                                                                                                                                     
tttGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCt                                                                                                                                                       <  1:5634561/150‑1 (MQ=255)
tttGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCt                                                                                                                                                       <  1:4819520/150‑1 (MQ=255)
 ttGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTg                                                                                                                                                      >  2:802023/1‑150 (MQ=255)
   gCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGAt                                                                                                                                                    <  2:2637157/150‑1 (MQ=255)
      tGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAg                                                                                                                                                 <  1:676335/150‑1 (MQ=255)
              gtTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCCTAGGCCGGATAAGGCGCACGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTtgt                                                                                                                                         <  2:238522/150‑1 (MQ=255)
               ttCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTtgtg                                                                                                                                        <  1:922566/150‑1 (MQ=255)
                 gctttataTTAGGTGTTCTCGGATGGACGACCCGTAGGCCTGATAAGGCTCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGcc                                                                                                                                      <  2:5815439/143‑1 (MQ=11)
                  cTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCg                                                                                                                                     >  1:1868876/1‑150 (MQ=255)
                   tGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGc                                                                                                                                    >  2:5417213/1‑150 (MQ=255)
                   tGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGc                                                                                                                                    >  2:2748470/1‑150 (MQ=255)
                    gTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCa                                                                                                                                   >  2:3266066/1‑150 (MQ=255)
                     tAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCAt                                                                                                                                  >  2:4047880/1‑150 (MQ=255)
                      aaaTTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATc                                                                                                                                 <  1:4628492/150‑1 (MQ=255)
                         ttCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCgg                                                                                                                              <  2:1390543/150‑1 (MQ=255)
                          tCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGc                                                                                                                             >  1:1815836/1‑150 (MQ=255)
                            ggTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGcaa                                                                                                                           >  2:3739668/1‑150 (MQ=255)
                             gtgtTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACCACTATTGCCGACCTGTAGGCCGGAGAAGGCACTTGTGCCGCATCCGGcact                                                                                                                          >  2:2545924/1‑148 (MQ=255)
                                  gtCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATg                                                                                                                     <  1:1372416/150‑1 (MQ=255)
                                   tCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGc                                                                                                                    <  2:3319537/150‑1 (MQ=255)
                                   tCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGc                                                                                                                    <  2:4739997/150‑1 (MQ=255)
                                   tCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTCATGCGACCCTGTCGCGACTAATCCGGCCTACACCTATTGCCGACCAGTAGGCCAGATAACGCCCTTCTGCCGCAACCGGCAAACAATGc                                                                                                                    >  1:5186402/1‑150 (MQ=14)
                                     ggATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCt                                                                                                                  >  1:4926217/1‑150 (MQ=255)
                                     ggATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCt                                                                                                                  <  2:3093024/150‑1 (MQ=255)
                                      gATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTg                                                                                                                 >  2:5736276/1‑150 (MQ=255)
                                       aTGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGa                                                                                                                >  1:206753/1‑150 (MQ=255)
                                           aCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCCATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCCCTTGTCCCGCATCCGGCCATCAATGCCTGAGGCg                                                                                                            >  2:5323206/1‑150 (MQ=14)
                                             gACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGAc                                                                                                          <  1:5410423/150‑1 (MQ=255)
                                              aCCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACg                                                                                                         <  1:2285982/150‑1 (MQ=255)
                                                  gTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCTATCATTGCCTGATGCGACGCTGTCGCGTATTATCAGGGCTAGAACTATTGCCGACCTGTAGGCCTGATTTGGCACTTGTGCCGCATCGGGCAATCAATGCCTGTTGCGACGCTGt                                                                                                     >  1:4554427/1‑150 (MQ=14)
                                                   tAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTc                                                                                                    <  1:5718895/150‑1 (MQ=39)
                                                    aGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg                                                                                                   <  2:5978464/150‑1 (MQ=39)
                                                      gCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAGCTATTGCCGACCTGTAGGCCTGATAAGGCACTTGGGCCGCGTCCGGCAATCAATGCCTGATGCGACGCGGTcgcg                                                                                                 >  2:3897575/1‑150 (MQ=255)
                                                      gCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgcg                                                                                                 <  1:5028610/150‑1 (MQ=39)
                                                        cGGATGCGGCGCGAGCGCCTTATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTc                                                                                               >  1:3461913/1‑150 (MQ=11)
                                                         ggATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCt                                                                                              <  1:1718306/150‑1 (MQ=39)
                                                             aaGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATc                                                                                          <  1:4450638/150‑1 (MQ=39)
                                                              aGGCGCTCGCGCCGCATCACGCAATCAATGCCTGATGCGAAGCTGTCGCGTCTTATCAGGCCTACAACGATTGCCGACCTGTAGGCCTGAGAAGGCCCTGGTGCCGCCTCCGGCGCTCTATGCCTGCTGCGACGCGGGCGCGTCTTagcg                                                                                         >  1:2922541/1‑147 (MQ=14)
                                                               ggCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAg                                                                                        <  2:5560179/150‑1 (MQ=39)
                                                                gcgcTCGCGCCGCATCCGGCACTCAATGCCGGATGCGACGCTGTCGCTTCTTCTCATGACGACAACTGTTCACGACCACTACGCCACCTCAGGCCCTAGAGCCGCATCCGGCATGCGATGCCAGATGCGACGCTCTCGCGTGTTATCacg                                                                                       >  2:4507446/1‑148 (MQ=255)
                                                                gcgcTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAgg                                                                                       <  2:1276389/150‑1 (MQ=38)
                                                                 cgcTCGCGCAGCATCCGGCAATCACTGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGc                                                                                      <  2:121610/150‑1 (MQ=255)
                                                                  gcTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGcc                                                                                     <  2:1815836/150‑1 (MQ=17)
                                                                   cTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCt                                                                                    <  2:854242/150‑1 (MQ=35)
                                                                        gccgcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAAc                                                                               >  2:2055369/1‑150 (MQ=32)
                                                                          cgcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTg                                                                             <  1:5530270/150‑1 (MQ=32)
                                                                            cATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACTACTGtt                                                                           <  2:3807974/150‑1 (MQ=17)
                                                                            cATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGtt                                                                           <  1:4678783/150‑1 (MQ=32)
                                                                             aTCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTg                                                                          <  1:5738500/150‑1 (MQ=32)
                                                                                 ggCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGa                                                                      >  1:3992606/1‑150 (MQ=32)
                                                                                 ggCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGa                                                                      >  1:1116785/1‑150 (MQ=32)
                                                                                 ggCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGa                                                                      <  1:5296636/150‑1 (MQ=32)
                                                                                 ggCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGa                                                                      >  2:5217079/1‑150 (MQ=32)
                                                                                      tcaatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGt                                                                 <  1:3926910/150‑1 (MQ=34)
                                                                                           gCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGcc                                                            <  1:1027750/150‑1 (MQ=34)
                                                                                            ccTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCg                                                           >  2:1490854/1‑150 (MQ=34)
                                                                                              tGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGa                                                         <  1:5817446/150‑1 (MQ=34)
                                                                                                aTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATa                                                       <  1:2371552/150‑1 (MQ=34)
                                                                                                  gCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAg                                                     <  1:94792/150‑1 (MQ=34)
                                                                                                  gCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAg                                                     <  2:5646366/150‑1 (MQ=34)
                                                                                                     aCGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCa                                                  <  1:5946822/150‑1 (MQ=35)
                                                                                                     aCGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCAGTAGGCCGGATAAGGCa                                                  >  2:4319688/1‑150 (MQ=17)
                                                                                                          gTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTtgt                                             <  1:3266066/150‑1 (MQ=37)
                                                                                                               gTCTTATCAGGCCTACAACGATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGc                                        <  1:280180/150‑1 (MQ=37)
                                                                                                                tCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATGCGGCACAAGTGcctta                                       >  1:410670/1‑147 (MQ=11)
                                                                                                                    aTCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGc                                                                         >  1:4488054/1‑112 (MQ=32)
                                                                                                                    aTCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATGCGGCACAAGTGCCTTATCCg                                   <  2:4627691/150‑1 (MQ=11)
                                                                                                                     tCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCgg                                  >  1:3638414/1‑150 (MQ=37)
                                                                                                                     tCAGGACGACAACTATTGGCGACCTGTAGGCCTGATAAGGCTCTTGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCgg                                  <  2:5068921/150‑1 (MQ=11)
                                                                                                                        ggCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaa                               <  1:2678892/150‑1 (MQ=37)
                                                                                                                        ggCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCAGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaa                               <  1:3066699/150‑1 (MQ=37)
                                                                                                                         gCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGTCGCATCCGGcaat                              >  2:4966956/1‑150 (MQ=37)
                                                                                                                         gCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaat                              <  2:2214701/150‑1 (MQ=37)
                                                                                                                          ccTACAACTCGTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatc                             <  2:3509999/150‑1 (MQ=21)
                                                                                                                            tACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCAGTAGGCCGGATAAGGCACGTGAGCCGCATCCGGcgatcaa                           >  2:5185522/1‑150 (MQ=255)
                                                                                                                             acaccgaTTGCCGACCAGTAGGCCTGATAAGGCTCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTCTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatcaat                          <  2:4569655/144‑1 (MQ=11)
                                                                                                                             aCAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatcaat                          <  1:3140702/150‑1 (MQ=37)
                                                                                                                             aCAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatcaat                          <  1:4296990/150‑1 (MQ=37)
                                                                                                                               aaCTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCt                                                                                                                  >  1:5383982/1‑60 (MQ=35)
                                                                                                                               aaCTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCCCTTGTGCCGCATCCGGCAATCAGTGc                        >  2:2899277/1‑150 (MQ=255)
                                                                                                                               aaCTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGc                        <  2:1163827/150‑1 (MQ=37)
                                                                                                                                  tATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGCTAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTg                     >  2:3419120/1‑150 (MQ=25)
                                                                                                                                    ttGCCGACCTGTAGGCCTGATAAGGCTCTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGGTGCCGACCTGTAGGCCGGATAAGGCACTTGTGACGCATCCGGCAATCAATGCCTGac                   <  1:807659/150‑2 (MQ=255)
                                                                                                                                       ccGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCGTCCGGCACTCAATGCCTGATGCg                >  2:704086/1‑150 (MQ=21)
                                                                                                                                       ccGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCg                >  2:1595366/1‑150 (MQ=34)
                                                                                                                                       ccGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCg                >  1:5906639/1‑150 (MQ=34)
                                                                                                                                       ccGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCg                >  2:2997465/1‑150 (MQ=34)
                                                                                                                                        cGACCTGTAGGCCTGATAAGGCACTTGTGACGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGa               >  2:3772776/1‑150 (MQ=21)
                                                                                                                                         gACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGAc              <  1:1175474/150‑1 (MQ=34)
                                                                                                                                          aCCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACg             >  2:4405194/1‑150 (MQ=34)
                                                                                                                                            cTGTAGGCCTGATAAGGCACTTGTGCCACATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCt           <  1:874561/150‑1 (MQ=21)
                                                                                                                                             tGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCAGTAGGCCGGATAAGGCACTTGAGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg          >  2:3600002/1‑150 (MQ=21)
                                                                                                                                                  gCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCAGTAGGCCGGATAAGGCACTAGAGCCGCATCCGGCAATCAATGCCAGATGCGACGCttaagcg     >  2:4184505/1‑144 (MQ=11)
                                                                                                                                                  gCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGACTACAACTGTTGCCGACCAGTAGGCCGGATACGGCCCTAGAGCCGCATCCGGCATTCAATTCCAGATGCGACGcacacggg     >  1:3749516/1‑143 (MQ=11)
                                                                                                                                                  gCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGAAGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgcg     >  1:2717818/1‑150 (MQ=21)
                                                                                                                                                  gCCTGATAAGGCACTTGTGACGCATCCGGCAATCAATGCCTGATGCTACGATGTCTCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGAACTTGTGCCGCATCAGGCAATCAATGCCTGATGCGACGCTGTagcg     >  1:1526298/1‑150 (MQ=11)
                                                                                                                                                    cTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTc   >  1:2152574/1‑150 (MQ=33)
                                                                                                                                                    cTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTc   >  1:3242037/1‑150 (MQ=33)
                                                                                                                                                     tGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCt  >  1:4221384/1‑150 (MQ=32)
                                                                                                                                                     tGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGGGCCGCATCCGGCACTCAATGCCTGATGCGACGCTGTCGCTTCt  >  2:1127181/1‑150 (MQ=12)
                                                                                                                                                     |                                                                                                                                                     
TTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCT  >  NC_000913/4094266‑4094564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: